Morphological and functional changes in rats incisor triggered by chronic exposure to fluoride

Autores
Fontanetti, Pablo Alejandro; Interlandi, Victoria; Ponce, Rubén Hugo; Gallará, Raquel Vivian; Centeno, Viviana Andrea; Tirao, Germán Alfredo; Rigalli, Alfredo
Año de publicación
2014
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Fil: Fontanetti, Pablo Alejandro. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Metodología de la Investigación en Ciencias de la Salud; Argentina
Fil: Interlandi, Victoria. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; Argentina
Fil: Ponce, Rubén Hugo. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; Argentina
Fil: Tirao, Germán Alfredo. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía, Física y Computación; Argentina
Fil: Rigalli, Alfredo. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina
Fil: Gallará, Raquel Vivian. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; Argentina
Fil: Centeno, Viviana Andrea. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; Argentina
It is well known that excessive intake of fluoride (F-) during dentinogenesis causes dental fluorosis. However the molecular mechanism underlying fluorosis remains unclear. Connexins (Cxs) are important to control growth and cell differentiation of dental tissues.The aim of the present study was to assess the impact of chronic exposure of F- on Cxs expression in dental pulp, and on morphometric parameters of adult rat mandible and incisors. Three groups of male Wistar rats (22 days-old) were given water containing: a) 0.3 mg/L (Control), b) 10 mg/L and c) 50 mg/L of NaF for eight weeks. Incisor pulp homogenates were prepared for determination of Cx32, Cx43 and Cx45 gene expression,
using semi-quantitative RT-PCR, and of ALP activity. Morphometric parameters of mandible and incisors were determined on radiographs. Histomorphometric parameters were evaluated with Image Pro Plus 6.1 software. Results were analyzed by ANOVA and Bonferroni´s test. Expression of Cx43 increased with exposure to F- in a dose-dependent manner. Cx32 mRNA levels were higher than controls in the 10 mg/L NaF group only; Cx45 mRNA levels were lower in groups given 10 and 50 mg/L of NaF than in controls. ALP activity was higher in both high-NaF dose groups compared to the control group (p<0.05). Lower incisor diameter was lower in the 50 mg/L NaF than in the control group (p<0.01). None of the mandibular growth or histomorphometric parameters were affected by NaF treatment. In conclusion, fluorotic alterations in rat incisor were associated with differential expression of Cxs and ALP activity in pulp tissue. The observed changes may have a stimulating effect on dentin mineralization.
Fil: Fontanetti, Pablo Alejandro. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Metodología de la Investigación en Ciencias de la Salud; Argentina
Fil: Interlandi, Victoria. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; Argentina
Fil: Ponce, Rubén Hugo. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; Argentina
Fil: Tirao, Germán Alfredo. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía, Física y Computación; Argentina
Fil: Rigalli, Alfredo. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina
Fil: Gallará, Raquel Vivian. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; Argentina
Fil: Centeno, Viviana Andrea. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; Argentina
Materia
Fluoruros
Pulpa dental
Odontoblastos
Conexinas
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Repositorio Digital Universitario (UNC)
Institución
Universidad Nacional de Córdoba
OAI Identificador
oai:rdu.unc.edu.ar:11086/20057
Acceder
id RDUUNC_698ee61fa6cb22bab3bb6ceaf6b7c621
oai_identifier_str oai:rdu.unc.edu.ar:11086/20057
network_acronym_str RDUUNC
repository_id_str 2572
network_name_str Repositorio Digital Universitario (UNC)
spelling Morphological and functional changes in rats incisor triggered by chronic exposure to fluorideFontanetti, Pablo AlejandroInterlandi, VictoriaPonce, Rubén HugoGallará, Raquel VivianCenteno, Viviana AndreaTirao, Germán AlfredoRigalli, AlfredoFluorurosPulpa dentalOdontoblastosConexinasFil: Fontanetti, Pablo Alejandro. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Metodología de la Investigación en Ciencias de la Salud; ArgentinaFil: Interlandi, Victoria. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; ArgentinaFil: Ponce, Rubén Hugo. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; ArgentinaFil: Tirao, Germán Alfredo. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía, Física y Computación; ArgentinaFil: Rigalli, Alfredo. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; ArgentinaFil: Gallará, Raquel Vivian. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; ArgentinaFil: Centeno, Viviana Andrea. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; ArgentinaIt is well known that excessive intake of fluoride (F-) during dentinogenesis causes dental fluorosis. However the molecular mechanism underlying fluorosis remains unclear. Connexins (Cxs) are important to control growth and cell differentiation of dental tissues.The aim of the present study was to assess the impact of chronic exposure of F- on Cxs expression in dental pulp, and on morphometric parameters of adult rat mandible and incisors. Three groups of male Wistar rats (22 days-old) were given water containing: a) 0.3 mg/L (Control), b) 10 mg/L and c) 50 mg/L of NaF for eight weeks. Incisor pulp homogenates were prepared for determination of Cx32, Cx43 and Cx45 gene expression,<br />using semi-quantitative RT-PCR, and of ALP activity. Morphometric parameters of mandible and incisors were determined on radiographs. Histomorphometric parameters were evaluated with Image Pro Plus 6.1 software. Results were analyzed by ANOVA and Bonferroni´s test. Expression of Cx43 increased with exposure to F- in a dose-dependent manner. Cx32 mRNA levels were higher than controls in the 10 mg/L NaF group only; Cx45 mRNA levels were lower in groups given 10 and 50 mg/L of NaF than in controls. ALP activity was higher in both high-NaF dose groups compared to the control group (p&lt;0.05). Lower incisor diameter was lower in the 50 mg/L NaF than in the control group (p&lt;0.01). None of the mandibular growth or histomorphometric parameters were affected by NaF treatment. In conclusion, fluorotic alterations in rat incisor were associated with differential expression of Cxs and ALP activity in pulp tissue. The observed changes may have a stimulating effect on dentin mineralization.Fil: Fontanetti, Pablo Alejandro. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Metodología de la Investigación en Ciencias de la Salud; ArgentinaFil: Interlandi, Victoria. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; ArgentinaFil: Ponce, Rubén Hugo. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; ArgentinaFil: Tirao, Germán Alfredo. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía, Física y Computación; ArgentinaFil: Rigalli, Alfredo. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; ArgentinaFil: Gallará, Raquel Vivian. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; ArgentinaFil: Centeno, Viviana Andrea. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; ArgentinaElsevier2014info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfhttp://hdl.handle.net/11086/20057enginfo:eu-repo/semantics/openAccessreponame:Repositorio Digital Universitario (UNC)instname:Universidad Nacional de Córdobainstacron:UNC2025-09-29T13:44:29Zoai:rdu.unc.edu.ar:11086/20057Institucionalhttps://rdu.unc.edu.ar/Universidad públicaNo correspondehttp://rdu.unc.edu.ar/oai/snrdoca.unc@gmail.comArgentinaNo correspondeNo correspondeNo correspondeopendoar:25722025-09-29 13:44:29.877Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdobafalse
dc.title.none.fl_str_mv Morphological and functional changes in rats incisor triggered by chronic exposure to fluoride
title Morphological and functional changes in rats incisor triggered by chronic exposure to fluoride
spellingShingle Morphological and functional changes in rats incisor triggered by chronic exposure to fluoride
Fontanetti, Pablo Alejandro
Fluoruros
Pulpa dental
Odontoblastos
Conexinas
title_short Morphological and functional changes in rats incisor triggered by chronic exposure to fluoride
title_full Morphological and functional changes in rats incisor triggered by chronic exposure to fluoride
title_fullStr Morphological and functional changes in rats incisor triggered by chronic exposure to fluoride
title_full_unstemmed Morphological and functional changes in rats incisor triggered by chronic exposure to fluoride
title_sort Morphological and functional changes in rats incisor triggered by chronic exposure to fluoride
dc.creator.none.fl_str_mv Fontanetti, Pablo Alejandro
Interlandi, Victoria
Ponce, Rubén Hugo
Gallará, Raquel Vivian
Centeno, Viviana Andrea
Tirao, Germán Alfredo
Rigalli, Alfredo
author Fontanetti, Pablo Alejandro
author_facet Fontanetti, Pablo Alejandro
Interlandi, Victoria
Ponce, Rubén Hugo
Gallará, Raquel Vivian
Centeno, Viviana Andrea
Tirao, Germán Alfredo
Rigalli, Alfredo
author_role author
author2 Interlandi, Victoria
Ponce, Rubén Hugo
Gallará, Raquel Vivian
Centeno, Viviana Andrea
Tirao, Germán Alfredo
Rigalli, Alfredo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Fluoruros
Pulpa dental
Odontoblastos
Conexinas
topic Fluoruros
Pulpa dental
Odontoblastos
Conexinas
dc.description.none.fl_txt_mv Fil: Fontanetti, Pablo Alejandro. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Metodología de la Investigación en Ciencias de la Salud; Argentina
Fil: Interlandi, Victoria. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; Argentina
Fil: Ponce, Rubén Hugo. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; Argentina
Fil: Tirao, Germán Alfredo. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía, Física y Computación; Argentina
Fil: Rigalli, Alfredo. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina
Fil: Gallará, Raquel Vivian. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; Argentina
Fil: Centeno, Viviana Andrea. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; Argentina
It is well known that excessive intake of fluoride (F-) during dentinogenesis causes dental fluorosis. However the molecular mechanism underlying fluorosis remains unclear. Connexins (Cxs) are important to control growth and cell differentiation of dental tissues.The aim of the present study was to assess the impact of chronic exposure of F- on Cxs expression in dental pulp, and on morphometric parameters of adult rat mandible and incisors. Three groups of male Wistar rats (22 days-old) were given water containing: a) 0.3 mg/L (Control), b) 10 mg/L and c) 50 mg/L of NaF for eight weeks. Incisor pulp homogenates were prepared for determination of Cx32, Cx43 and Cx45 gene expression,<br />using semi-quantitative RT-PCR, and of ALP activity. Morphometric parameters of mandible and incisors were determined on radiographs. Histomorphometric parameters were evaluated with Image Pro Plus 6.1 software. Results were analyzed by ANOVA and Bonferroni´s test. Expression of Cx43 increased with exposure to F- in a dose-dependent manner. Cx32 mRNA levels were higher than controls in the 10 mg/L NaF group only; Cx45 mRNA levels were lower in groups given 10 and 50 mg/L of NaF than in controls. ALP activity was higher in both high-NaF dose groups compared to the control group (p&lt;0.05). Lower incisor diameter was lower in the 50 mg/L NaF than in the control group (p&lt;0.01). None of the mandibular growth or histomorphometric parameters were affected by NaF treatment. In conclusion, fluorotic alterations in rat incisor were associated with differential expression of Cxs and ALP activity in pulp tissue. The observed changes may have a stimulating effect on dentin mineralization.
Fil: Fontanetti, Pablo Alejandro. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Metodología de la Investigación en Ciencias de la Salud; Argentina
Fil: Interlandi, Victoria. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; Argentina
Fil: Ponce, Rubén Hugo. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; Argentina
Fil: Tirao, Germán Alfredo. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía, Física y Computación; Argentina
Fil: Rigalli, Alfredo. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Ósea; Argentina
Fil: Gallará, Raquel Vivian. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; Argentina
Fil: Centeno, Viviana Andrea. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Química Biológica A; Argentina
description Fil: Fontanetti, Pablo Alejandro. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Metodología de la Investigación en Ciencias de la Salud; Argentina
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
format conferenceObject
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11086/20057
url http://hdl.handle.net/11086/20057
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositorio Digital Universitario (UNC)
instname:Universidad Nacional de Córdoba
instacron:UNC
reponame_str Repositorio Digital Universitario (UNC)
collection Repositorio Digital Universitario (UNC)
instname_str Universidad Nacional de Córdoba
instacron_str UNC
institution UNC
repository.name.fl_str_mv Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdoba
repository.mail.fl_str_mv oca.unc@gmail.com
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score 13.070432

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