Octopamine is required for successful reproduction in the classical insect model, Rhodnius prolixus

Autores
Leyria, Jimena; Orchard, Ian; Lange, Angela B.
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Impact Factor (IF) 2023 (2024 update): 2.9. Revisado por pares
Fil: Leyria, Jimena. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina.
Fil: Leyria, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina.
Fil: Orchard, Ian. University of Toronto Mississauga, Department of Biology; Canada.
Fil: Lange, Angela B. University of Toronto Mississauga, Department of Biology; Canada.
In insects, biogenic amines function as neurotransmitters, neuromodulators, and neurohormones, influencing various behaviors, including those related to reproduction such as response to sex pheromones, oogenesis, oviposition, courtship, and mating. Octopamine (OA), an analog of the vertebrate norepinephrine, is synthesized from the biogenic amine tyramine by the enzyme tyramine β-hydroxylase (TβH). Here, we investigate the mechanisms and target genes underlying the role of OA in successful reproduction in females of Rhodnius prolixus, a vector of Chagas disease, by downregulating TβH mRNA expression (thereby reducing OA content) using RNA interference (RNAi), and in vivo and ex vivo application of OA. Injection of females with dsTβH impairs successful reproduction at least in part, by decreasing the transcript expression of enzymes involved in juvenile hormone biosynthesis, the primary hormone for oogenesis in R. prolixus, thereby interfering with oogenesis, ovulation and oviposition. This study offers valuable insights into the involvement of OA for successful reproduction in R. prolixus females. Understanding the reproductive biology of R. prolixus is crucial in a medical context for controlling the spread of the disease.
info:eu-repo/semantics/publishedVersion
Fil: Leyria, Jimena. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina.
Fil: Leyria, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina.
Fil: Orchard, Ian. University of Toronto Mississauga, Department of Biology; Canada.
Fil: Lange, Angela B. University of Toronto Mississauga, Department of Biology; Canada.
Materia
Biogenic amines
Reproduction
Octopamine (OA)
RNA interference (RNAi)
Rhodnius prolixus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Repositorio Digital Universitario (UNC)
Institución
Universidad Nacional de Córdoba
OAI Identificador
oai:rdu.unc.edu.ar:11086/552763
Acceder
id RDUUNC_2ff930fe44f0993fa000d487420d3230
oai_identifier_str oai:rdu.unc.edu.ar:11086/552763
network_acronym_str RDUUNC
repository_id_str 2572
network_name_str Repositorio Digital Universitario (UNC)
spelling Octopamine is required for successful reproduction in the classical insect model, Rhodnius prolixusLeyria, JimenaOrchard, IanLange, Angela B.Biogenic aminesReproductionOctopamine (OA)RNA interference (RNAi)Rhodnius prolixusImpact Factor (IF) 2023 (2024 update): 2.9. Revisado por paresFil: Leyria, Jimena. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina.Fil: Leyria, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina.Fil: Orchard, Ian. University of Toronto Mississauga, Department of Biology; Canada.Fil: Lange, Angela B. University of Toronto Mississauga, Department of Biology; Canada.In insects, biogenic amines function as neurotransmitters, neuromodulators, and neurohormones, influencing various behaviors, including those related to reproduction such as response to sex pheromones, oogenesis, oviposition, courtship, and mating. Octopamine (OA), an analog of the vertebrate norepinephrine, is synthesized from the biogenic amine tyramine by the enzyme tyramine β-hydroxylase (TβH). Here, we investigate the mechanisms and target genes underlying the role of OA in successful reproduction in females of Rhodnius prolixus, a vector of Chagas disease, by downregulating TβH mRNA expression (thereby reducing OA content) using RNA interference (RNAi), and in vivo and ex vivo application of OA. Injection of females with dsTβH impairs successful reproduction at least in part, by decreasing the transcript expression of enzymes involved in juvenile hormone biosynthesis, the primary hormone for oogenesis in R. prolixus, thereby interfering with oogenesis, ovulation and oviposition. This study offers valuable insights into the involvement of OA for successful reproduction in R. prolixus females. Understanding the reproductive biology of R. prolixus is crucial in a medical context for controlling the spread of the disease.info:eu-repo/semantics/publishedVersionFil: Leyria, Jimena. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina.Fil: Leyria, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina.Fil: Orchard, Ian. University of Toronto Mississauga, Department of Biology; Canada.Fil: Lange, Angela B. University of Toronto Mississauga, Department of Biology; Canada.Wolfgang Blenau, University of Leipzig. Faculty of Life Sciences: Universitat Leipzig Fakultat fur Lebenswissenschaften, GERMANYhttps://orcid.org/0000-0002-9145-5523https://orcid.org/0000-0002-4491-7924https://orcid.org/0000-0003-2195-43162024-07-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfLeyria J, Orchard I, Lange AB (2024) Octopamine is required for successful reproduction in the classical insect model, Rhodnius prolixus. PLoS ONE 19(7): e0306611. https://doi.org/ 10.1371/journal.pone.0306611http://hdl.handle.net/11086/5527631932-6203https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0306611https://pubmed.ncbi.nlm.nih.gov/38995904/http://doi.org/10.1371/journal.pone.0306611enginfo:eu-repo/semantics/openAccessreponame:Repositorio Digital Universitario (UNC)instname:Universidad Nacional de Córdobainstacron:UNC2025-09-04T12:32:17Zoai:rdu.unc.edu.ar:11086/552763Institucionalhttps://rdu.unc.edu.ar/Universidad públicaNo correspondehttp://rdu.unc.edu.ar/oai/snrdoca.unc@gmail.comArgentinaNo correspondeNo correspondeNo correspondeopendoar:25722025-09-04 12:32:17.838Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdobafalse
dc.title.none.fl_str_mv Octopamine is required for successful reproduction in the classical insect model, Rhodnius prolixus
title Octopamine is required for successful reproduction in the classical insect model, Rhodnius prolixus
spellingShingle Octopamine is required for successful reproduction in the classical insect model, Rhodnius prolixus
Leyria, Jimena
Biogenic amines
Reproduction
Octopamine (OA)
RNA interference (RNAi)
Rhodnius prolixus
title_short Octopamine is required for successful reproduction in the classical insect model, Rhodnius prolixus
title_full Octopamine is required for successful reproduction in the classical insect model, Rhodnius prolixus
title_fullStr Octopamine is required for successful reproduction in the classical insect model, Rhodnius prolixus
title_full_unstemmed Octopamine is required for successful reproduction in the classical insect model, Rhodnius prolixus
title_sort Octopamine is required for successful reproduction in the classical insect model, Rhodnius prolixus
dc.creator.none.fl_str_mv Leyria, Jimena
Orchard, Ian
Lange, Angela B.
author Leyria, Jimena
author_facet Leyria, Jimena
Orchard, Ian
Lange, Angela B.
author_role author
author2 Orchard, Ian
Lange, Angela B.
author2_role author
author
dc.contributor.none.fl_str_mv https://orcid.org/0000-0002-9145-5523
https://orcid.org/0000-0002-4491-7924
https://orcid.org/0000-0003-2195-4316
dc.subject.none.fl_str_mv Biogenic amines
Reproduction
Octopamine (OA)
RNA interference (RNAi)
Rhodnius prolixus
topic Biogenic amines
Reproduction
Octopamine (OA)
RNA interference (RNAi)
Rhodnius prolixus
dc.description.none.fl_txt_mv Impact Factor (IF) 2023 (2024 update): 2.9. Revisado por pares
Fil: Leyria, Jimena. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina.
Fil: Leyria, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina.
Fil: Orchard, Ian. University of Toronto Mississauga, Department of Biology; Canada.
Fil: Lange, Angela B. University of Toronto Mississauga, Department of Biology; Canada.
In insects, biogenic amines function as neurotransmitters, neuromodulators, and neurohormones, influencing various behaviors, including those related to reproduction such as response to sex pheromones, oogenesis, oviposition, courtship, and mating. Octopamine (OA), an analog of the vertebrate norepinephrine, is synthesized from the biogenic amine tyramine by the enzyme tyramine β-hydroxylase (TβH). Here, we investigate the mechanisms and target genes underlying the role of OA in successful reproduction in females of Rhodnius prolixus, a vector of Chagas disease, by downregulating TβH mRNA expression (thereby reducing OA content) using RNA interference (RNAi), and in vivo and ex vivo application of OA. Injection of females with dsTβH impairs successful reproduction at least in part, by decreasing the transcript expression of enzymes involved in juvenile hormone biosynthesis, the primary hormone for oogenesis in R. prolixus, thereby interfering with oogenesis, ovulation and oviposition. This study offers valuable insights into the involvement of OA for successful reproduction in R. prolixus females. Understanding the reproductive biology of R. prolixus is crucial in a medical context for controlling the spread of the disease.
info:eu-repo/semantics/publishedVersion
Fil: Leyria, Jimena. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina.
Fil: Leyria, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina.
Fil: Orchard, Ian. University of Toronto Mississauga, Department of Biology; Canada.
Fil: Lange, Angela B. University of Toronto Mississauga, Department of Biology; Canada.
description Impact Factor (IF) 2023 (2024 update): 2.9. Revisado por pares
publishDate 2024
dc.date.none.fl_str_mv 2024-07-12
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
status_str publishedVersion
format article
dc.identifier.none.fl_str_mv Leyria J, Orchard I, Lange AB (2024) Octopamine is required for successful reproduction in the classical insect model, Rhodnius prolixus. PLoS ONE 19(7): e0306611. https://doi.org/ 10.1371/journal.pone.0306611
http://hdl.handle.net/11086/552763
1932-6203
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0306611
https://pubmed.ncbi.nlm.nih.gov/38995904/
http://doi.org/10.1371/journal.pone.0306611
identifier_str_mv Leyria J, Orchard I, Lange AB (2024) Octopamine is required for successful reproduction in the classical insect model, Rhodnius prolixus. PLoS ONE 19(7): e0306611. https://doi.org/ 10.1371/journal.pone.0306611
1932-6203
url http://hdl.handle.net/11086/552763
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0306611
https://pubmed.ncbi.nlm.nih.gov/38995904/
http://doi.org/10.1371/journal.pone.0306611
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wolfgang Blenau, University of Leipzig. Faculty of Life Sciences: Universitat Leipzig Fakultat fur Lebenswissenschaften, GERMANY
publisher.none.fl_str_mv Wolfgang Blenau, University of Leipzig. Faculty of Life Sciences: Universitat Leipzig Fakultat fur Lebenswissenschaften, GERMANY
dc.source.none.fl_str_mv reponame:Repositorio Digital Universitario (UNC)
instname:Universidad Nacional de Córdoba
instacron:UNC
reponame_str Repositorio Digital Universitario (UNC)
collection Repositorio Digital Universitario (UNC)
instname_str Universidad Nacional de Córdoba
instacron_str UNC
institution UNC
repository.name.fl_str_mv Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdoba
repository.mail.fl_str_mv oca.unc@gmail.com
_version_ 1842349634572255232
score 13.13397

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