Proteomic Analysis of Climatic Keratopathy Droplets

Autores
Menegay, Michelle; DeMia, Lee; Tabbara, Khalid F.; Cafaro, Thamara A.; Urrets Zavalía, Julio; Serra, Horacio M.; Bhattacharya, Sanjoy K.
Año de publicación
2008
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
PURPOSE. To identify the proteins in the corneal droplets of climatic droplet keratopathy (CDK), a disease that results in the formation of droplets on the cornea. Progressive accumulation of droplets in CDK leads to visual loss. METHODS. Proteomic mass spectrometry of the CDK specimens was performed after fractionation of proteins in 4% to 20% SDS-polyacrylamide gels. Droplets were derived from two human donors. Immunohistochemistry with antibodies was performed to confirm the presence of identified proteins on donor tissues from patients with CDK and control subjects. RESULTS. Proteomic analyses revealed identification of 105 proteins in CDK specimens. Immunohistochemical analyses confirmed localization of annexin A2 and glyceraldehyde 3-dehydrogenase (GAPDH), proteins identified by proteomic analyses in CDK specimens. The proteins were subjected to analyses with the Kyoto Encyclopedia of Genes and Genomes (KEGG) Database which showed that a few biochemical pathways were more frequent for the identified proteins. CONCLUSIONS. Approximately 105 proteins were identified in CDK specimens, and a subset of them was confirmed by immunohistochemistry. Several of these may play a role in fibril or deposit formation.
Fil: Menegay, Michelle. Bascom Palmer Eye Institute, University of Miami, Miami, Florida
Fil: DeMia, Lee. Bascom Palmer Eye Institute, University of Miami, Miami, Florida
Fil: Tabbara, Khalid F. The Eye Center and The Eye Foundation for Research in Ophthalmology, Riyadh, Saudi Arabia
Fil: Cafaro, Thamara A. CIBICI (Centro de Investigaciones en Bioquimica Clinica e Inmunologia), Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba
Fil: Urrets Zavalía, Julio. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina
Fil: Serra, Horacio M. CIBICI (Centro de Investigaciones en Bioquimica Clinica e Inmunologia), Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba
Fil: Bhattacharya, Sanjoy K. Bascom Palmer Eye Institute, University of Miami, Miami, Florida
Fuente
Menegay, Michelle, DeMia, Lee, Tabbara, Khalid F., Cafaro, Thamara A., Urrets Zavalía, Julio ORCID: https://orcid.org/0000-0003-2267-2783 , Serra, Horacio M. and Bhattacharya, Sanjoy K. (2008) Proteomic Analysis of Climatic Keratopathy Droplets. Investigative Ophthalmology & Visual Science, 49 (7). ISSN 2829-2837
Materia
RE Oftalmología
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
Repositorio
Producción Académica (UCC)
Institución
Universidad Católica de Córdoba
OAI Identificador
oai:pa.bibdigital.uccor.edu.ar:4806
Acceder
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oai_identifier_str oai:pa.bibdigital.uccor.edu.ar:4806
network_acronym_str PAUCC
repository_id_str 2718
network_name_str Producción Académica (UCC)
spelling Proteomic Analysis of Climatic Keratopathy DropletsMenegay, MichelleDeMia, LeeTabbara, Khalid F.Cafaro, Thamara A.Urrets Zavalía, JulioSerra, Horacio M.Bhattacharya, Sanjoy K.RE OftalmologíaPURPOSE. To identify the proteins in the corneal droplets of climatic droplet keratopathy (CDK), a disease that results in the formation of droplets on the cornea. Progressive accumulation of droplets in CDK leads to visual loss. METHODS. Proteomic mass spectrometry of the CDK specimens was performed after fractionation of proteins in 4% to 20% SDS-polyacrylamide gels. Droplets were derived from two human donors. Immunohistochemistry with antibodies was performed to confirm the presence of identified proteins on donor tissues from patients with CDK and control subjects. RESULTS. Proteomic analyses revealed identification of 105 proteins in CDK specimens. Immunohistochemical analyses confirmed localization of annexin A2 and glyceraldehyde 3-dehydrogenase (GAPDH), proteins identified by proteomic analyses in CDK specimens. The proteins were subjected to analyses with the Kyoto Encyclopedia of Genes and Genomes (KEGG) Database which showed that a few biochemical pathways were more frequent for the identified proteins. CONCLUSIONS. Approximately 105 proteins were identified in CDK specimens, and a subset of them was confirmed by immunohistochemistry. Several of these may play a role in fibril or deposit formation.Fil: Menegay, Michelle. Bascom Palmer Eye Institute, University of Miami, Miami, FloridaFil: DeMia, Lee. Bascom Palmer Eye Institute, University of Miami, Miami, FloridaFil: Tabbara, Khalid F. The Eye Center and The Eye Foundation for Research in Ophthalmology, Riyadh, Saudi ArabiaFil: Cafaro, Thamara A. CIBICI (Centro de Investigaciones en Bioquimica Clinica e Inmunologia), Facultad de Ciencias Quimicas, Universidad Nacional de CordobaFil: Urrets Zavalía, Julio. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; ArgentinaFil: Serra, Horacio M. CIBICI (Centro de Investigaciones en Bioquimica Clinica e Inmunologia), Facultad de Ciencias Quimicas, Universidad Nacional de CordobaFil: Bhattacharya, Sanjoy K. Bascom Palmer Eye Institute, University of Miami, Miami, Florida2008-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://pa.bibdigital.ucc.edu.ar/4806/1/A_Menegay.pdf Menegay, Michelle, DeMia, Lee, Tabbara, Khalid F., Cafaro, Thamara A., Urrets Zavalía, Julio ORCID: https://orcid.org/0000-0003-2267-2783 <https://orcid.org/0000-0003-2267-2783>, Serra, Horacio M. and Bhattacharya, Sanjoy K. (2008) Proteomic Analysis of Climatic Keratopathy Droplets. Investigative Ophthalmology & Visual Science, 49 (7). ISSN 2829-2837 reponame:Producción Académica (UCC)instname:Universidad Católica de Córdobaspahttp://pa.bibdigital.ucc.edu.ar/4806/https://iovs.arvojournals.org/article.aspx?articleid=2125493info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1167/iovs.07-1438info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es2025-09-29T14:29:57Zoai:pa.bibdigital.uccor.edu.ar:4806instacron:UCCInstitucionalhttp://pa.bibdigital.uccor.edu.ar/Universidad privadaNo correspondehttp://pa.bibdigital.uccor.edu.ar/cgi/oai2bibdir@uccor.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:27182025-09-29 14:29:57.662Producción Académica (UCC) - Universidad Católica de Córdobafalse
dc.title.none.fl_str_mv Proteomic Analysis of Climatic Keratopathy Droplets
title Proteomic Analysis of Climatic Keratopathy Droplets
spellingShingle Proteomic Analysis of Climatic Keratopathy Droplets
Menegay, Michelle
RE Oftalmología
title_short Proteomic Analysis of Climatic Keratopathy Droplets
title_full Proteomic Analysis of Climatic Keratopathy Droplets
title_fullStr Proteomic Analysis of Climatic Keratopathy Droplets
title_full_unstemmed Proteomic Analysis of Climatic Keratopathy Droplets
title_sort Proteomic Analysis of Climatic Keratopathy Droplets
dc.creator.none.fl_str_mv Menegay, Michelle
DeMia, Lee
Tabbara, Khalid F.
Cafaro, Thamara A.
Urrets Zavalía, Julio
Serra, Horacio M.
Bhattacharya, Sanjoy K.
author Menegay, Michelle
author_facet Menegay, Michelle
DeMia, Lee
Tabbara, Khalid F.
Cafaro, Thamara A.
Urrets Zavalía, Julio
Serra, Horacio M.
Bhattacharya, Sanjoy K.
author_role author
author2 DeMia, Lee
Tabbara, Khalid F.
Cafaro, Thamara A.
Urrets Zavalía, Julio
Serra, Horacio M.
Bhattacharya, Sanjoy K.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv RE Oftalmología
topic RE Oftalmología
dc.description.none.fl_txt_mv PURPOSE. To identify the proteins in the corneal droplets of climatic droplet keratopathy (CDK), a disease that results in the formation of droplets on the cornea. Progressive accumulation of droplets in CDK leads to visual loss. METHODS. Proteomic mass spectrometry of the CDK specimens was performed after fractionation of proteins in 4% to 20% SDS-polyacrylamide gels. Droplets were derived from two human donors. Immunohistochemistry with antibodies was performed to confirm the presence of identified proteins on donor tissues from patients with CDK and control subjects. RESULTS. Proteomic analyses revealed identification of 105 proteins in CDK specimens. Immunohistochemical analyses confirmed localization of annexin A2 and glyceraldehyde 3-dehydrogenase (GAPDH), proteins identified by proteomic analyses in CDK specimens. The proteins were subjected to analyses with the Kyoto Encyclopedia of Genes and Genomes (KEGG) Database which showed that a few biochemical pathways were more frequent for the identified proteins. CONCLUSIONS. Approximately 105 proteins were identified in CDK specimens, and a subset of them was confirmed by immunohistochemistry. Several of these may play a role in fibril or deposit formation.
Fil: Menegay, Michelle. Bascom Palmer Eye Institute, University of Miami, Miami, Florida
Fil: DeMia, Lee. Bascom Palmer Eye Institute, University of Miami, Miami, Florida
Fil: Tabbara, Khalid F. The Eye Center and The Eye Foundation for Research in Ophthalmology, Riyadh, Saudi Arabia
Fil: Cafaro, Thamara A. CIBICI (Centro de Investigaciones en Bioquimica Clinica e Inmunologia), Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba
Fil: Urrets Zavalía, Julio. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina
Fil: Serra, Horacio M. CIBICI (Centro de Investigaciones en Bioquimica Clinica e Inmunologia), Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba
Fil: Bhattacharya, Sanjoy K. Bascom Palmer Eye Institute, University of Miami, Miami, Florida
description PURPOSE. To identify the proteins in the corneal droplets of climatic droplet keratopathy (CDK), a disease that results in the formation of droplets on the cornea. Progressive accumulation of droplets in CDK leads to visual loss. METHODS. Proteomic mass spectrometry of the CDK specimens was performed after fractionation of proteins in 4% to 20% SDS-polyacrylamide gels. Droplets were derived from two human donors. Immunohistochemistry with antibodies was performed to confirm the presence of identified proteins on donor tissues from patients with CDK and control subjects. RESULTS. Proteomic analyses revealed identification of 105 proteins in CDK specimens. Immunohistochemical analyses confirmed localization of annexin A2 and glyceraldehyde 3-dehydrogenase (GAPDH), proteins identified by proteomic analyses in CDK specimens. The proteins were subjected to analyses with the Kyoto Encyclopedia of Genes and Genomes (KEGG) Database which showed that a few biochemical pathways were more frequent for the identified proteins. CONCLUSIONS. Approximately 105 proteins were identified in CDK specimens, and a subset of them was confirmed by immunohistochemistry. Several of these may play a role in fibril or deposit formation.
publishDate 2008
dc.date.none.fl_str_mv 2008-12-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://pa.bibdigital.ucc.edu.ar/4806/1/A_Menegay.pdf
url http://pa.bibdigital.ucc.edu.ar/4806/1/A_Menegay.pdf
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv http://pa.bibdigital.ucc.edu.ar/4806/
https://iovs.arvojournals.org/article.aspx?articleid=2125493
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1167/iovs.07-1438
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Menegay, Michelle, DeMia, Lee, Tabbara, Khalid F., Cafaro, Thamara A., Urrets Zavalía, Julio ORCID: https://orcid.org/0000-0003-2267-2783 <https://orcid.org/0000-0003-2267-2783>, Serra, Horacio M. and Bhattacharya, Sanjoy K. (2008) Proteomic Analysis of Climatic Keratopathy Droplets. Investigative Ophthalmology & Visual Science, 49 (7). ISSN 2829-2837
reponame:Producción Académica (UCC)
instname:Universidad Católica de Córdoba
reponame_str Producción Académica (UCC)
collection Producción Académica (UCC)
instname_str Universidad Católica de Córdoba
repository.name.fl_str_mv Producción Académica (UCC) - Universidad Católica de Córdoba
repository.mail.fl_str_mv bibdir@uccor.edu.ar
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