Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples

Autores
Fernandez, Jorge Gastón; Fernández Baldo, Martín Alejandro; Muñoz, Claudio Javier; Salinas, Eloy; Raba, Julio; Sanz Ferramola, Maria Isabel
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: To detect Botrytis cinerea (B. cinerea) latent infections on apples before storage, which is essential for effective control strategies in the fruit postharvest industry. Methods: In the present study, a polymerase chain reaction detection method, based on primers designed on B. cinerea transposable elements (boty and flipper) and intergenic spacer region as internal control, were utilized to reveal the presence of symptomless infections on apple fruits. This molecular method proved to be highly specific and sensitive in detecting latent infections. It revealed the presence of the pathogen in 83% of the samples from infected apples with 104 conidia/ mL, whereas those infected with 106 conidia/mL detected 94% as compared to the traditional method that revealed the pathogen in 40% and 66% of the samples inoculated with 104 and 106 conidia/mL respectively. Furthermore, the method characterized B. cinerea as subpopulation transposa-type by the presence of the transposable elements boty and flipper Results: The results obtained from DNA quantification method were compared with enzymelinked immunosorbent assay and these studies showed good correlation. Therefore our method has important advantages compared with others detection methods for B. cinerea, because the proposed methodology allowed distinguishes between its two subpopulations (vacuma and transposa) and this would allow establish possible appropriate control strategies. Conclusions: Finally, the method can be an interesting alternative for its possible application in the phytosanitary programs of the fruit industry worldwide.
EEA Mendoza
Fil: Fernández, Jorge Gastón. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; Argentina
Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentina
Fil: Muñoz, Claudio Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Salinas, Eloy. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentina
Fil: Sanz Ferramola, Maria Isabel. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentina
Fuente
Journal of Coastal Life Medicine 2 (2) : 125-131 (2014)
Materia
Botrytis Cinerea
Manzana
Hongos
Enfermedades de las Plantas
Enfermedades Postcosecha
Infecciones Latentes
Apples
Fungi
Plant Diseases
Postharvest Decay
Latent Infections
Enfermedades durante Almacenamiento
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4297
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4297
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless applesFernandez, Jorge GastónFernández Baldo, Martín AlejandroMuñoz, Claudio JavierSalinas, EloyRaba, JulioSanz Ferramola, Maria IsabelBotrytis CinereaManzanaHongosEnfermedades de las PlantasEnfermedades PostcosechaInfecciones LatentesApplesFungiPlant DiseasesPostharvest DecayLatent InfectionsEnfermedades durante AlmacenamientoObjective: To detect Botrytis cinerea (B. cinerea) latent infections on apples before storage, which is essential for effective control strategies in the fruit postharvest industry. Methods: In the present study, a polymerase chain reaction detection method, based on primers designed on B. cinerea transposable elements (boty and flipper) and intergenic spacer region as internal control, were utilized to reveal the presence of symptomless infections on apple fruits. This molecular method proved to be highly specific and sensitive in detecting latent infections. It revealed the presence of the pathogen in 83% of the samples from infected apples with 104 conidia/ mL, whereas those infected with 106 conidia/mL detected 94% as compared to the traditional method that revealed the pathogen in 40% and 66% of the samples inoculated with 104 and 106 conidia/mL respectively. Furthermore, the method characterized B. cinerea as subpopulation transposa-type by the presence of the transposable elements boty and flipper Results: The results obtained from DNA quantification method were compared with enzymelinked immunosorbent assay and these studies showed good correlation. Therefore our method has important advantages compared with others detection methods for B. cinerea, because the proposed methodology allowed distinguishes between its two subpopulations (vacuma and transposa) and this would allow establish possible appropriate control strategies. Conclusions: Finally, the method can be an interesting alternative for its possible application in the phytosanitary programs of the fruit industry worldwide.EEA MendozaFil: Fernández, Jorge Gastón. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; ArgentinaFil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; ArgentinaFil: Muñoz, Claudio Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Salinas, Eloy. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; ArgentinaFil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; ArgentinaFil: Sanz Ferramola, Maria Isabel. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; ArgentinaHainan Medical University2019-01-21T11:45:10Z2019-01-21T11:45:10Z2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://oaji.net/articles/2015/2154-1445415270.pdfhttp://hdl.handle.net/20.500.12123/42972309-52882309-6152https://doi.org/10.12980/JCLM.2.2014JCLM-2013-0005Journal of Coastal Life Medicine 2 (2) : 125-131 (2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:47:46Zoai:localhost:20.500.12123/4297instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:46.926INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples
title Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples
spellingShingle Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples
Fernandez, Jorge Gastón
Botrytis Cinerea
Manzana
Hongos
Enfermedades de las Plantas
Enfermedades Postcosecha
Infecciones Latentes
Apples
Fungi
Plant Diseases
Postharvest Decay
Latent Infections
Enfermedades durante Almacenamiento
title_short Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples
title_full Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples
title_fullStr Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples
title_full_unstemmed Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples
title_sort Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples
dc.creator.none.fl_str_mv Fernandez, Jorge Gastón
Fernández Baldo, Martín Alejandro
Muñoz, Claudio Javier
Salinas, Eloy
Raba, Julio
Sanz Ferramola, Maria Isabel
author Fernandez, Jorge Gastón
author_facet Fernandez, Jorge Gastón
Fernández Baldo, Martín Alejandro
Muñoz, Claudio Javier
Salinas, Eloy
Raba, Julio
Sanz Ferramola, Maria Isabel
author_role author
author2 Fernández Baldo, Martín Alejandro
Muñoz, Claudio Javier
Salinas, Eloy
Raba, Julio
Sanz Ferramola, Maria Isabel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Botrytis Cinerea
Manzana
Hongos
Enfermedades de las Plantas
Enfermedades Postcosecha
Infecciones Latentes
Apples
Fungi
Plant Diseases
Postharvest Decay
Latent Infections
Enfermedades durante Almacenamiento
topic Botrytis Cinerea
Manzana
Hongos
Enfermedades de las Plantas
Enfermedades Postcosecha
Infecciones Latentes
Apples
Fungi
Plant Diseases
Postharvest Decay
Latent Infections
Enfermedades durante Almacenamiento
dc.description.none.fl_txt_mv Objective: To detect Botrytis cinerea (B. cinerea) latent infections on apples before storage, which is essential for effective control strategies in the fruit postharvest industry. Methods: In the present study, a polymerase chain reaction detection method, based on primers designed on B. cinerea transposable elements (boty and flipper) and intergenic spacer region as internal control, were utilized to reveal the presence of symptomless infections on apple fruits. This molecular method proved to be highly specific and sensitive in detecting latent infections. It revealed the presence of the pathogen in 83% of the samples from infected apples with 104 conidia/ mL, whereas those infected with 106 conidia/mL detected 94% as compared to the traditional method that revealed the pathogen in 40% and 66% of the samples inoculated with 104 and 106 conidia/mL respectively. Furthermore, the method characterized B. cinerea as subpopulation transposa-type by the presence of the transposable elements boty and flipper Results: The results obtained from DNA quantification method were compared with enzymelinked immunosorbent assay and these studies showed good correlation. Therefore our method has important advantages compared with others detection methods for B. cinerea, because the proposed methodology allowed distinguishes between its two subpopulations (vacuma and transposa) and this would allow establish possible appropriate control strategies. Conclusions: Finally, the method can be an interesting alternative for its possible application in the phytosanitary programs of the fruit industry worldwide.
EEA Mendoza
Fil: Fernández, Jorge Gastón. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; Argentina
Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentina
Fil: Muñoz, Claudio Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Salinas, Eloy. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentina
Fil: Sanz Ferramola, Maria Isabel. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentina
description Objective: To detect Botrytis cinerea (B. cinerea) latent infections on apples before storage, which is essential for effective control strategies in the fruit postharvest industry. Methods: In the present study, a polymerase chain reaction detection method, based on primers designed on B. cinerea transposable elements (boty and flipper) and intergenic spacer region as internal control, were utilized to reveal the presence of symptomless infections on apple fruits. This molecular method proved to be highly specific and sensitive in detecting latent infections. It revealed the presence of the pathogen in 83% of the samples from infected apples with 104 conidia/ mL, whereas those infected with 106 conidia/mL detected 94% as compared to the traditional method that revealed the pathogen in 40% and 66% of the samples inoculated with 104 and 106 conidia/mL respectively. Furthermore, the method characterized B. cinerea as subpopulation transposa-type by the presence of the transposable elements boty and flipper Results: The results obtained from DNA quantification method were compared with enzymelinked immunosorbent assay and these studies showed good correlation. Therefore our method has important advantages compared with others detection methods for B. cinerea, because the proposed methodology allowed distinguishes between its two subpopulations (vacuma and transposa) and this would allow establish possible appropriate control strategies. Conclusions: Finally, the method can be an interesting alternative for its possible application in the phytosanitary programs of the fruit industry worldwide.
publishDate 2014
dc.date.none.fl_str_mv 2014
2019-01-21T11:45:10Z
2019-01-21T11:45:10Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://oaji.net/articles/2015/2154-1445415270.pdf
http://hdl.handle.net/20.500.12123/4297
2309-5288
2309-6152
https://doi.org/10.12980/JCLM.2.2014JCLM-2013-0005
url http://oaji.net/articles/2015/2154-1445415270.pdf
http://hdl.handle.net/20.500.12123/4297
https://doi.org/10.12980/JCLM.2.2014JCLM-2013-0005
identifier_str_mv 2309-5288
2309-6152
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hainan Medical University
publisher.none.fl_str_mv Hainan Medical University
dc.source.none.fl_str_mv Journal of Coastal Life Medicine 2 (2) : 125-131 (2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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