Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples

Autores
Fernández, Jorge Gastón; Fernández Baldo, Martín Alejandro; Muñoz, Claudio Javier; Salinas, Eloy; Raba, Julio; Sanz Ferramola, Maria Isabel
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Detection of Botrytis cinerea latent infections on apples before storage is essential for effective control strategies in the fruit postharvest industry. In the present study a PCR detection method, based on primers designed on B. cinerea transposable elements (boty and flipper) and intergenic spacer (IGS) region as internal control were utilized to reveal the presence of symptomless infections on apple fruits. The method molecular proved to be highly specific and sensitive in detecting latent infections, revealed the presence of the pathogen in 83% of the samples from infected apples with 104 conidia/mL, whereas those infected with 106 conidia/mL detected 94% as compared to the traditional method that revealed the pathogen in 40 and 66% of the samples inoculated with 104 and 106 conidia/mL respectively. Furthermore, the method characterized B. cinerea as subpopulation transposa-type by the presence of the transposable elements boty and flipper The results obtained of DNA quantification method were compared with ELISA assay and these studies showed good correlation. Therefore our method has important advantages compared with others detection methods for B. cinerea, because the proposed methodology allowed distinguishes between its two subpopulations (vacuma and transposa) and this would allow establish possible control appropriate strategies. Finally, the method can be an interesting alternative for its possible application in the phytosanitary programs of the fruit industry worldwide.
Fil: Fernández, Jorge Gastón. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; Argentina
Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Muñoz, Claudio Javier. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Cuyo Mendoza - San Juan. Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Salinas, Eloy. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Sanz Ferramola, Maria Isabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Materia
BOTRYTIS CINEREA
LATENT INFECTION
TRANSPOSABLE ELEMENTS
SYMPTOMLESS APPLES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/5828
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/5828
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless applesFernández, Jorge GastónFernández Baldo, Martín AlejandroMuñoz, Claudio JavierSalinas, EloyRaba, JulioSanz Ferramola, Maria IsabelBOTRYTIS CINEREALATENT INFECTIONTRANSPOSABLE ELEMENTSSYMPTOMLESS APPLEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Detection of Botrytis cinerea latent infections on apples before storage is essential for effective control strategies in the fruit postharvest industry. In the present study a PCR detection method, based on primers designed on B. cinerea transposable elements (boty and flipper) and intergenic spacer (IGS) region as internal control were utilized to reveal the presence of symptomless infections on apple fruits. The method molecular proved to be highly specific and sensitive in detecting latent infections, revealed the presence of the pathogen in 83% of the samples from infected apples with 104 conidia/mL, whereas those infected with 106 conidia/mL detected 94% as compared to the traditional method that revealed the pathogen in 40 and 66% of the samples inoculated with 104 and 106 conidia/mL respectively. Furthermore, the method characterized B. cinerea as subpopulation transposa-type by the presence of the transposable elements boty and flipper The results obtained of DNA quantification method were compared with ELISA assay and these studies showed good correlation. Therefore our method has important advantages compared with others detection methods for B. cinerea, because the proposed methodology allowed distinguishes between its two subpopulations (vacuma and transposa) and this would allow establish possible control appropriate strategies. Finally, the method can be an interesting alternative for its possible application in the phytosanitary programs of the fruit industry worldwide.Fil: Fernández, Jorge Gastón. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; ArgentinaFil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Muñoz, Claudio Javier. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Cuyo Mendoza - San Juan. Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Salinas, Eloy. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Sanz Ferramola, Maria Isabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaHainan Medical University2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/5828Fernández, Jorge Gastón; Fernández Baldo, Martín Alejandro; Muñoz, Claudio Javier; Salinas, Eloy; Raba, Julio; et al.; Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples; Hainan Medical University; Journal of Coastal Life Medicine; 2; 2; 2-2014; 125-1312309-61522309-5288enginfo:eu-repo/semantics/altIdentifier/ark/http://www.jclmm.com/qk/20142/9.pdfinfo:eu-repo/semantics/altIdentifier/doi/10.12980/JCLM.2.2014JCLM-2013-0005info:eu-repo/semantics/altIdentifier/url/10.12980/JCLM.2.2014JCLM-2013-0005info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:00Zoai:ri.conicet.gov.ar:11336/5828instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:00.319CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples
title Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples
spellingShingle Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples
Fernández, Jorge Gastón
BOTRYTIS CINEREA
LATENT INFECTION
TRANSPOSABLE ELEMENTS
SYMPTOMLESS APPLES
title_short Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples
title_full Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples
title_fullStr Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples
title_full_unstemmed Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples
title_sort Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples
dc.creator.none.fl_str_mv Fernández, Jorge Gastón
Fernández Baldo, Martín Alejandro
Muñoz, Claudio Javier
Salinas, Eloy
Raba, Julio
Sanz Ferramola, Maria Isabel
author Fernández, Jorge Gastón
author_facet Fernández, Jorge Gastón
Fernández Baldo, Martín Alejandro
Muñoz, Claudio Javier
Salinas, Eloy
Raba, Julio
Sanz Ferramola, Maria Isabel
author_role author
author2 Fernández Baldo, Martín Alejandro
Muñoz, Claudio Javier
Salinas, Eloy
Raba, Julio
Sanz Ferramola, Maria Isabel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BOTRYTIS CINEREA
LATENT INFECTION
TRANSPOSABLE ELEMENTS
SYMPTOMLESS APPLES
topic BOTRYTIS CINEREA
LATENT INFECTION
TRANSPOSABLE ELEMENTS
SYMPTOMLESS APPLES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Detection of Botrytis cinerea latent infections on apples before storage is essential for effective control strategies in the fruit postharvest industry. In the present study a PCR detection method, based on primers designed on B. cinerea transposable elements (boty and flipper) and intergenic spacer (IGS) region as internal control were utilized to reveal the presence of symptomless infections on apple fruits. The method molecular proved to be highly specific and sensitive in detecting latent infections, revealed the presence of the pathogen in 83% of the samples from infected apples with 104 conidia/mL, whereas those infected with 106 conidia/mL detected 94% as compared to the traditional method that revealed the pathogen in 40 and 66% of the samples inoculated with 104 and 106 conidia/mL respectively. Furthermore, the method characterized B. cinerea as subpopulation transposa-type by the presence of the transposable elements boty and flipper The results obtained of DNA quantification method were compared with ELISA assay and these studies showed good correlation. Therefore our method has important advantages compared with others detection methods for B. cinerea, because the proposed methodology allowed distinguishes between its two subpopulations (vacuma and transposa) and this would allow establish possible control appropriate strategies. Finally, the method can be an interesting alternative for its possible application in the phytosanitary programs of the fruit industry worldwide.
Fil: Fernández, Jorge Gastón. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; Argentina
Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Muñoz, Claudio Javier. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Cuyo Mendoza - San Juan. Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Salinas, Eloy. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Sanz Ferramola, Maria Isabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
description Detection of Botrytis cinerea latent infections on apples before storage is essential for effective control strategies in the fruit postharvest industry. In the present study a PCR detection method, based on primers designed on B. cinerea transposable elements (boty and flipper) and intergenic spacer (IGS) region as internal control were utilized to reveal the presence of symptomless infections on apple fruits. The method molecular proved to be highly specific and sensitive in detecting latent infections, revealed the presence of the pathogen in 83% of the samples from infected apples with 104 conidia/mL, whereas those infected with 106 conidia/mL detected 94% as compared to the traditional method that revealed the pathogen in 40 and 66% of the samples inoculated with 104 and 106 conidia/mL respectively. Furthermore, the method characterized B. cinerea as subpopulation transposa-type by the presence of the transposable elements boty and flipper The results obtained of DNA quantification method were compared with ELISA assay and these studies showed good correlation. Therefore our method has important advantages compared with others detection methods for B. cinerea, because the proposed methodology allowed distinguishes between its two subpopulations (vacuma and transposa) and this would allow establish possible control appropriate strategies. Finally, the method can be an interesting alternative for its possible application in the phytosanitary programs of the fruit industry worldwide.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/5828
Fernández, Jorge Gastón; Fernández Baldo, Martín Alejandro; Muñoz, Claudio Javier; Salinas, Eloy; Raba, Julio; et al.; Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples; Hainan Medical University; Journal of Coastal Life Medicine; 2; 2; 2-2014; 125-131
2309-6152
2309-5288
url http://hdl.handle.net/11336/5828
identifier_str_mv Fernández, Jorge Gastón; Fernández Baldo, Martín Alejandro; Muñoz, Claudio Javier; Salinas, Eloy; Raba, Julio; et al.; Detection transposable elements in Botrytis cinerea in latent infection stage from symptomless apples; Hainan Medical University; Journal of Coastal Life Medicine; 2; 2; 2-2014; 125-131
2309-6152
2309-5288
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/ark/http://www.jclmm.com/qk/20142/9.pdf
info:eu-repo/semantics/altIdentifier/doi/10.12980/JCLM.2.2014JCLM-2013-0005
info:eu-repo/semantics/altIdentifier/url/10.12980/JCLM.2.2014JCLM-2013-0005
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hainan Medical University
publisher.none.fl_str_mv Hainan Medical University
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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