Fungal-derived extracts induce resistance against Botrytis cinerea in Arabidopsis thaliana
- Autores
- Hael Conrad, Veronica; Tomas-Grau, Rodrigo H.; Moschen, Sebastian Nicolas; Requena-Serra, F.J.; Díaz Ricci, Juan Carlos; Salazar, Sergio Miguel
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Development of a sustainable agriculture is a current trend searching for alternative and more environmentally friendly strategies to control crop diseases. The necrotrophic fungus Botrytis cinerea, the causal agent of grey mould, affects crops pre- and post-harvest and cause huge economical losses. Microorganisms and microbially-derived elicitors are known to induce plant defences. Here, we study two fungal extracts derived from Colletotrichum acutatum (isolate M11). The crude extract, called CF (culture filtrate) and the semi-purified fraction ACF (axenic culture filtrate) consist in a blend of fungal-derived elicitors. Their activities were evaluated in the plant-pathogen system Arabidopsis thaliana-B. cinerea. Both CF and ACF induced local and systemic resistance, rendering A. thaliana resistant against grey mould disease. The extracts showed no antifungal activity since no inhibition of mycelium growth, changes in hyphal ultrastructure or damage in cell membrane permeability were observed. These findings would indicate that the protective effect was indirect and it was found that CF and ACF induced up-regulation of the defence-related genes PR1, WRKY70 and ERF6, as well as giving local callose deposition in treated leaves. Both fungal extracts have the potential to be used as a main component of a biologically based disease control product with activity against grey mould, with the advantage that the purification process of CF is simpler than for ACF.
EEA Famaillá
Fil: Hael-Conrad, Verónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Tomas-Grau, R.H. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Moschen, Sebastian Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina.
Fil: Requena-Serra, F.J. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Diaz Ricci, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina.
Fil: Salazar, Sergio Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina - Fuente
- European Journal of Plant Pathology 158 : 45–58 (2020)
- Materia
-
Enfermedades de las Plantas
Control de Enfermedades
Botrytis cinerea
Arabidopsis thaliana
Extractos
Plant Diseases
Diseases Control
Extracts - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
.jpg)
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/7759
Ver los metadatos del registro completo
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Fungal-derived extracts induce resistance against Botrytis cinerea in Arabidopsis thalianaHael Conrad, VeronicaTomas-Grau, Rodrigo H.Moschen, Sebastian NicolasRequena-Serra, F.J.Díaz Ricci, Juan CarlosSalazar, Sergio MiguelEnfermedades de las PlantasControl de EnfermedadesBotrytis cinereaArabidopsis thalianaExtractosPlant DiseasesDiseases ControlExtractsDevelopment of a sustainable agriculture is a current trend searching for alternative and more environmentally friendly strategies to control crop diseases. The necrotrophic fungus Botrytis cinerea, the causal agent of grey mould, affects crops pre- and post-harvest and cause huge economical losses. Microorganisms and microbially-derived elicitors are known to induce plant defences. Here, we study two fungal extracts derived from Colletotrichum acutatum (isolate M11). The crude extract, called CF (culture filtrate) and the semi-purified fraction ACF (axenic culture filtrate) consist in a blend of fungal-derived elicitors. Their activities were evaluated in the plant-pathogen system Arabidopsis thaliana-B. cinerea. Both CF and ACF induced local and systemic resistance, rendering A. thaliana resistant against grey mould disease. The extracts showed no antifungal activity since no inhibition of mycelium growth, changes in hyphal ultrastructure or damage in cell membrane permeability were observed. These findings would indicate that the protective effect was indirect and it was found that CF and ACF induced up-regulation of the defence-related genes PR1, WRKY70 and ERF6, as well as giving local callose deposition in treated leaves. Both fungal extracts have the potential to be used as a main component of a biologically based disease control product with activity against grey mould, with the advantage that the purification process of CF is simpler than for ACF.EEA FamailláFil: Hael-Conrad, Verónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Tomas-Grau, R.H. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Moschen, Sebastian Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina.Fil: Requena-Serra, F.J. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Diaz Ricci, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina.Fil: Salazar, Sergio Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; ArgentinaSpringer2020-08-24T13:07:40Z2020-08-24T13:07:40Z2020-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7759https://link.springer.com/article/10.1007/s10658-020-02054-10929-18731573-8469https://doi.org/10.1007/s10658-020-02054-1European Journal of Plant Pathology 158 : 45–58 (2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:48:36Zoai:localhost:20.500.12123/7759instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:37.019INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
| dc.title.none.fl_str_mv |
Fungal-derived extracts induce resistance against Botrytis cinerea in Arabidopsis thaliana |
| title |
Fungal-derived extracts induce resistance against Botrytis cinerea in Arabidopsis thaliana |
| spellingShingle |
Fungal-derived extracts induce resistance against Botrytis cinerea in Arabidopsis thaliana Hael Conrad, Veronica Enfermedades de las Plantas Control de Enfermedades Botrytis cinerea Arabidopsis thaliana Extractos Plant Diseases Diseases Control Extracts |
| title_short |
Fungal-derived extracts induce resistance against Botrytis cinerea in Arabidopsis thaliana |
| title_full |
Fungal-derived extracts induce resistance against Botrytis cinerea in Arabidopsis thaliana |
| title_fullStr |
Fungal-derived extracts induce resistance against Botrytis cinerea in Arabidopsis thaliana |
| title_full_unstemmed |
Fungal-derived extracts induce resistance against Botrytis cinerea in Arabidopsis thaliana |
| title_sort |
Fungal-derived extracts induce resistance against Botrytis cinerea in Arabidopsis thaliana |
| dc.creator.none.fl_str_mv |
Hael Conrad, Veronica Tomas-Grau, Rodrigo H. Moschen, Sebastian Nicolas Requena-Serra, F.J. Díaz Ricci, Juan Carlos Salazar, Sergio Miguel |
| author |
Hael Conrad, Veronica |
| author_facet |
Hael Conrad, Veronica Tomas-Grau, Rodrigo H. Moschen, Sebastian Nicolas Requena-Serra, F.J. Díaz Ricci, Juan Carlos Salazar, Sergio Miguel |
| author_role |
author |
| author2 |
Tomas-Grau, Rodrigo H. Moschen, Sebastian Nicolas Requena-Serra, F.J. Díaz Ricci, Juan Carlos Salazar, Sergio Miguel |
| author2_role |
author author author author author |
| dc.subject.none.fl_str_mv |
Enfermedades de las Plantas Control de Enfermedades Botrytis cinerea Arabidopsis thaliana Extractos Plant Diseases Diseases Control Extracts |
| topic |
Enfermedades de las Plantas Control de Enfermedades Botrytis cinerea Arabidopsis thaliana Extractos Plant Diseases Diseases Control Extracts |
| dc.description.none.fl_txt_mv |
Development of a sustainable agriculture is a current trend searching for alternative and more environmentally friendly strategies to control crop diseases. The necrotrophic fungus Botrytis cinerea, the causal agent of grey mould, affects crops pre- and post-harvest and cause huge economical losses. Microorganisms and microbially-derived elicitors are known to induce plant defences. Here, we study two fungal extracts derived from Colletotrichum acutatum (isolate M11). The crude extract, called CF (culture filtrate) and the semi-purified fraction ACF (axenic culture filtrate) consist in a blend of fungal-derived elicitors. Their activities were evaluated in the plant-pathogen system Arabidopsis thaliana-B. cinerea. Both CF and ACF induced local and systemic resistance, rendering A. thaliana resistant against grey mould disease. The extracts showed no antifungal activity since no inhibition of mycelium growth, changes in hyphal ultrastructure or damage in cell membrane permeability were observed. These findings would indicate that the protective effect was indirect and it was found that CF and ACF induced up-regulation of the defence-related genes PR1, WRKY70 and ERF6, as well as giving local callose deposition in treated leaves. Both fungal extracts have the potential to be used as a main component of a biologically based disease control product with activity against grey mould, with the advantage that the purification process of CF is simpler than for ACF. EEA Famaillá Fil: Hael-Conrad, Verónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Tomas-Grau, R.H. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina Fil: Moschen, Sebastian Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina. Fil: Requena-Serra, F.J. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina Fil: Diaz Ricci, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Fil: Salazar, Sergio Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina |
| description |
Development of a sustainable agriculture is a current trend searching for alternative and more environmentally friendly strategies to control crop diseases. The necrotrophic fungus Botrytis cinerea, the causal agent of grey mould, affects crops pre- and post-harvest and cause huge economical losses. Microorganisms and microbially-derived elicitors are known to induce plant defences. Here, we study two fungal extracts derived from Colletotrichum acutatum (isolate M11). The crude extract, called CF (culture filtrate) and the semi-purified fraction ACF (axenic culture filtrate) consist in a blend of fungal-derived elicitors. Their activities were evaluated in the plant-pathogen system Arabidopsis thaliana-B. cinerea. Both CF and ACF induced local and systemic resistance, rendering A. thaliana resistant against grey mould disease. The extracts showed no antifungal activity since no inhibition of mycelium growth, changes in hyphal ultrastructure or damage in cell membrane permeability were observed. These findings would indicate that the protective effect was indirect and it was found that CF and ACF induced up-regulation of the defence-related genes PR1, WRKY70 and ERF6, as well as giving local callose deposition in treated leaves. Both fungal extracts have the potential to be used as a main component of a biologically based disease control product with activity against grey mould, with the advantage that the purification process of CF is simpler than for ACF. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-08-24T13:07:40Z 2020-08-24T13:07:40Z 2020-06 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/20.500.12123/7759 https://link.springer.com/article/10.1007/s10658-020-02054-1 0929-1873 1573-8469 https://doi.org/10.1007/s10658-020-02054-1 |
| url |
http://hdl.handle.net/20.500.12123/7759 https://link.springer.com/article/10.1007/s10658-020-02054-1 https://doi.org/10.1007/s10658-020-02054-1 |
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0929-1873 1573-8469 |
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eng |
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eng |
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Springer |
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Springer |
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European Journal of Plant Pathology 158 : 45–58 (2020) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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tripaldi.nicolas@inta.gob.ar |
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