Target-site and non-target-site resistance mechanisms confer multiple resistance to glyphosate and 2,4-D in Carduus acanthoides

Autores
Palma Bautista, Candelario; Belluccini, Pablo Alberto; Vázquez García, José Guadalupe; Alcántara de la Cruz, Ricardo; Barro, Francisco; Portugal, João; de Prado, Rafael
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Carduus acanthoides L. is mainly a range-land weed, but in the 2010s has begun to invade GM crop production systems in Córdoba (Argentina), where glyphosate and 2,4-D have been commonly applied. In 2020, C. acanthoides was found with multiple resistance to these two herbicides. In this study, the mechanisms that confer multiple resistance to glyphosate and 2,4-D, were characterized in one resistant (R) population of C. acanthoides in comparison to a susceptible (S) population. No differences in 14C-herbicide absorption and translocation were observed between R and S populations. In addition, 14C-glyphosate was well translocated to the shoots (∼30%) and roots (∼16%) in both R and S plants, while most of 14C-2,4-D remained restricted in the treated leaf. Glyphosate metabolism did not contribute to resistance of the R population; however, as corroborated by malathion pretreatment, the mechanism of resistance to 2,4-D was enhanced metabolism (63% of the herbicide) mediated by cytochrome P450 (Cyt-P450). No differences were found in baseline EPSPS activity, copy number, and/or gene expression between the R and S populations, but a Pro-106-Ser mutation in EPSPS was present in the R population. Multiple resistances in the R population of C. acanthoides from Argentina were governed by target site resistance (a Pro-106 mutation for glyphosate) and non-target site resistance (Cyt-P450-based metabolic resistance for 2,4-D) mechanisms. This is the first case of resistance to glyphosate and 2,4-D confirmed for this weed in the world.
EEA Marcos Juárez
Fil: Palma Bautista, Candelario. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España
Fil: Belluccini, Pablo Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Vázquez García, José G. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España
Fil: Alcántara de la Cruz, Ricardo. Universidade Federal de São Carlos. Centro de Ciências da Natureza; Brasil
Fil: Barro, Francisco. CSIC. Institute for Sustainable Agriculture; España
Fil: Portugal, João. Polytechnic Institute of Beja. Biosciences Department; Portugal
Fil: Portugal, João. Polytechnic Institute of Portalegre. VALORIZA-Research Centre for Endogenous Resource Valorization; Portugal
Fil: de Prado, Rafael. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España
Fuente
Pesticide Biochemistry and Physiology 191 : 105371 (April 2023)
Materia
Carduus acanthoides
Glifosato
Resistencia a los Herbicidas
Malezas
Glyphosate
2,4-D
Herbicide Resistance
Weeds
2,4-dichlorophenoxyacetic Acid
Ácido 2,4-diclorofenoxiacético
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/14182
Acceder
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oai_identifier_str oai:localhost:20.500.12123/14182
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network_name_str INTA Digital (INTA)
spelling Target-site and non-target-site resistance mechanisms confer multiple resistance to glyphosate and 2,4-D in Carduus acanthoidesPalma Bautista, CandelarioBelluccini, Pablo AlbertoVázquez García, José GuadalupeAlcántara de la Cruz, RicardoBarro, FranciscoPortugal, Joãode Prado, RafaelCarduus acanthoidesGlifosatoResistencia a los HerbicidasMalezasGlyphosate2,4-DHerbicide ResistanceWeeds2,4-dichlorophenoxyacetic AcidÁcido 2,4-diclorofenoxiacéticoCarduus acanthoides L. is mainly a range-land weed, but in the 2010s has begun to invade GM crop production systems in Córdoba (Argentina), where glyphosate and 2,4-D have been commonly applied. In 2020, C. acanthoides was found with multiple resistance to these two herbicides. In this study, the mechanisms that confer multiple resistance to glyphosate and 2,4-D, were characterized in one resistant (R) population of C. acanthoides in comparison to a susceptible (S) population. No differences in 14C-herbicide absorption and translocation were observed between R and S populations. In addition, 14C-glyphosate was well translocated to the shoots (∼30%) and roots (∼16%) in both R and S plants, while most of 14C-2,4-D remained restricted in the treated leaf. Glyphosate metabolism did not contribute to resistance of the R population; however, as corroborated by malathion pretreatment, the mechanism of resistance to 2,4-D was enhanced metabolism (63% of the herbicide) mediated by cytochrome P450 (Cyt-P450). No differences were found in baseline EPSPS activity, copy number, and/or gene expression between the R and S populations, but a Pro-106-Ser mutation in EPSPS was present in the R population. Multiple resistances in the R population of C. acanthoides from Argentina were governed by target site resistance (a Pro-106 mutation for glyphosate) and non-target site resistance (Cyt-P450-based metabolic resistance for 2,4-D) mechanisms. This is the first case of resistance to glyphosate and 2,4-D confirmed for this weed in the world.EEA Marcos JuárezFil: Palma Bautista, Candelario. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; EspañaFil: Belluccini, Pablo Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Vázquez García, José G. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; EspañaFil: Alcántara de la Cruz, Ricardo. Universidade Federal de São Carlos. Centro de Ciências da Natureza; BrasilFil: Barro, Francisco. CSIC. Institute for Sustainable Agriculture; EspañaFil: Portugal, João. Polytechnic Institute of Beja. Biosciences Department; PortugalFil: Portugal, João. Polytechnic Institute of Portalegre. VALORIZA-Research Centre for Endogenous Resource Valorization; PortugalFil: de Prado, Rafael. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; EspañaElsevier2023-03-08T14:13:54Z2023-03-08T14:13:54Z2023-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/14182https://www.sciencedirect.com/science/article/abs/pii/S00483575230003661095-99390048-3575https://doi.org/10.1016/j.pestbp.2023.105371Pesticide Biochemistry and Physiology 191 : 105371 (April 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:55Zoai:localhost:20.500.12123/14182instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:55.709INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Target-site and non-target-site resistance mechanisms confer multiple resistance to glyphosate and 2,4-D in Carduus acanthoides
title Target-site and non-target-site resistance mechanisms confer multiple resistance to glyphosate and 2,4-D in Carduus acanthoides
spellingShingle Target-site and non-target-site resistance mechanisms confer multiple resistance to glyphosate and 2,4-D in Carduus acanthoides
Palma Bautista, Candelario
Carduus acanthoides
Glifosato
Resistencia a los Herbicidas
Malezas
Glyphosate
2,4-D
Herbicide Resistance
Weeds
2,4-dichlorophenoxyacetic Acid
Ácido 2,4-diclorofenoxiacético
title_short Target-site and non-target-site resistance mechanisms confer multiple resistance to glyphosate and 2,4-D in Carduus acanthoides
title_full Target-site and non-target-site resistance mechanisms confer multiple resistance to glyphosate and 2,4-D in Carduus acanthoides
title_fullStr Target-site and non-target-site resistance mechanisms confer multiple resistance to glyphosate and 2,4-D in Carduus acanthoides
title_full_unstemmed Target-site and non-target-site resistance mechanisms confer multiple resistance to glyphosate and 2,4-D in Carduus acanthoides
title_sort Target-site and non-target-site resistance mechanisms confer multiple resistance to glyphosate and 2,4-D in Carduus acanthoides
dc.creator.none.fl_str_mv Palma Bautista, Candelario
Belluccini, Pablo Alberto
Vázquez García, José Guadalupe
Alcántara de la Cruz, Ricardo
Barro, Francisco
Portugal, João
de Prado, Rafael
author Palma Bautista, Candelario
author_facet Palma Bautista, Candelario
Belluccini, Pablo Alberto
Vázquez García, José Guadalupe
Alcántara de la Cruz, Ricardo
Barro, Francisco
Portugal, João
de Prado, Rafael
author_role author
author2 Belluccini, Pablo Alberto
Vázquez García, José Guadalupe
Alcántara de la Cruz, Ricardo
Barro, Francisco
Portugal, João
de Prado, Rafael
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Carduus acanthoides
Glifosato
Resistencia a los Herbicidas
Malezas
Glyphosate
2,4-D
Herbicide Resistance
Weeds
2,4-dichlorophenoxyacetic Acid
Ácido 2,4-diclorofenoxiacético
topic Carduus acanthoides
Glifosato
Resistencia a los Herbicidas
Malezas
Glyphosate
2,4-D
Herbicide Resistance
Weeds
2,4-dichlorophenoxyacetic Acid
Ácido 2,4-diclorofenoxiacético
dc.description.none.fl_txt_mv Carduus acanthoides L. is mainly a range-land weed, but in the 2010s has begun to invade GM crop production systems in Córdoba (Argentina), where glyphosate and 2,4-D have been commonly applied. In 2020, C. acanthoides was found with multiple resistance to these two herbicides. In this study, the mechanisms that confer multiple resistance to glyphosate and 2,4-D, were characterized in one resistant (R) population of C. acanthoides in comparison to a susceptible (S) population. No differences in 14C-herbicide absorption and translocation were observed between R and S populations. In addition, 14C-glyphosate was well translocated to the shoots (∼30%) and roots (∼16%) in both R and S plants, while most of 14C-2,4-D remained restricted in the treated leaf. Glyphosate metabolism did not contribute to resistance of the R population; however, as corroborated by malathion pretreatment, the mechanism of resistance to 2,4-D was enhanced metabolism (63% of the herbicide) mediated by cytochrome P450 (Cyt-P450). No differences were found in baseline EPSPS activity, copy number, and/or gene expression between the R and S populations, but a Pro-106-Ser mutation in EPSPS was present in the R population. Multiple resistances in the R population of C. acanthoides from Argentina were governed by target site resistance (a Pro-106 mutation for glyphosate) and non-target site resistance (Cyt-P450-based metabolic resistance for 2,4-D) mechanisms. This is the first case of resistance to glyphosate and 2,4-D confirmed for this weed in the world.
EEA Marcos Juárez
Fil: Palma Bautista, Candelario. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España
Fil: Belluccini, Pablo Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Vázquez García, José G. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España
Fil: Alcántara de la Cruz, Ricardo. Universidade Federal de São Carlos. Centro de Ciências da Natureza; Brasil
Fil: Barro, Francisco. CSIC. Institute for Sustainable Agriculture; España
Fil: Portugal, João. Polytechnic Institute of Beja. Biosciences Department; Portugal
Fil: Portugal, João. Polytechnic Institute of Portalegre. VALORIZA-Research Centre for Endogenous Resource Valorization; Portugal
Fil: de Prado, Rafael. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España
description Carduus acanthoides L. is mainly a range-land weed, but in the 2010s has begun to invade GM crop production systems in Córdoba (Argentina), where glyphosate and 2,4-D have been commonly applied. In 2020, C. acanthoides was found with multiple resistance to these two herbicides. In this study, the mechanisms that confer multiple resistance to glyphosate and 2,4-D, were characterized in one resistant (R) population of C. acanthoides in comparison to a susceptible (S) population. No differences in 14C-herbicide absorption and translocation were observed between R and S populations. In addition, 14C-glyphosate was well translocated to the shoots (∼30%) and roots (∼16%) in both R and S plants, while most of 14C-2,4-D remained restricted in the treated leaf. Glyphosate metabolism did not contribute to resistance of the R population; however, as corroborated by malathion pretreatment, the mechanism of resistance to 2,4-D was enhanced metabolism (63% of the herbicide) mediated by cytochrome P450 (Cyt-P450). No differences were found in baseline EPSPS activity, copy number, and/or gene expression between the R and S populations, but a Pro-106-Ser mutation in EPSPS was present in the R population. Multiple resistances in the R population of C. acanthoides from Argentina were governed by target site resistance (a Pro-106 mutation for glyphosate) and non-target site resistance (Cyt-P450-based metabolic resistance for 2,4-D) mechanisms. This is the first case of resistance to glyphosate and 2,4-D confirmed for this weed in the world.
publishDate 2023
dc.date.none.fl_str_mv 2023-03-08T14:13:54Z
2023-03-08T14:13:54Z
2023-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/14182
https://www.sciencedirect.com/science/article/abs/pii/S0048357523000366
1095-9939
0048-3575
https://doi.org/10.1016/j.pestbp.2023.105371
url http://hdl.handle.net/20.500.12123/14182
https://www.sciencedirect.com/science/article/abs/pii/S0048357523000366
https://doi.org/10.1016/j.pestbp.2023.105371
identifier_str_mv 1095-9939
0048-3575
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Pesticide Biochemistry and Physiology 191 : 105371 (April 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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