Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods

Autores
Soria, Maria Cecilia; Soria, Mario; Bueno, Dante Javier; Godano, Eduardo Ignacio; Gómez, S.C.; ViaButron, I.A.; Padin, V.M.; Rogé, Ariel Diego
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The performance of detection methods (culture methods and polymerase chain reaction assay) and plating media used in the same type of samples were determined as well as the specificity of PCR primers to detected Salmonella spp. contamination in layer hen farms. Also, the association of farm characteristics with Salmonella presence was evaluated. Environmental samples (feces, feed, drinking water, air, boot-swabs) and eggs were taken from 40 layer hen houses. Salmonella spp. was most detected in boot-swabs taken around the houses (30% and 35% by isolation and PCR, respectively) follow by fecal samples (15.2% and 13.6% by isolation and PCR, respectively). Eggs, drinking water, and air samples were negative for Salmonella detection. Salmonella Schwarzengrund and S. Enteritidis were the most isolated serotypes. For plating media, relative specificity was 1, and the relative sensitivity was greater for EF-18 agar than XLDT agar in feed and fecal samples. However, relative sensitivity was greater in XLDT agar than EF-18 agar for boot-swab samples. Agreement was between fair to good depending on the sample, and it was good between isolation and PCR (feces and boot-swabs), without agreement for feed samples. Salmonella spp. PCR was positive for all strains, while S. Typhimurium PCR was negative. Salmonella Enteritidis PCR used was not specific. Based in the multiple logistic regression analyses, categorization by counties was significant for Salmonella spp. presence (P-value = 0.010). This study shows the importance of considering different types of samples, plating media and detection methods during a Salmonella spp. monitoring study. In addition, it is important to incorporate the sampling of floors around the layer hen houses to learn if biosecurity measures should be strengthened to minimize the entry and spread of Salmonella in the houses. Also, the performance of some PCR methods and S. Enteritidis PCR should be improved, and biosecurity measures in hen farms must be reinforced in the region of more concentrated layer hen houses to reduce the probability of Salmonella spp. presence.
EEA Concepción del Uruguay
Fil: Soria, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina.
Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Godano, E.I. Tecnovo S.A.; Argentina
Fil: Gómez, S.C. Fundación ArgenINTA, Paraná; Argentina. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología, Sede Concepción del Uruguay; Argentina
Fil: ViaButron, I.A.. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina.
Fil: Padin, V.M. Instituto Nacional de Producción de Biológicos (INPB) - ANLIS “Dr Carlos G. Malbrán". Servicio de Antígenos y Antisueros; Argentina
Fil: Rogé, Ariel D. Instituto Nacional de Producción de Biológicos (INPB) - ANLIS “Dr Carlos G. Malbrán". Servicio de Antígenos y Antisueros; Argentina
Fuente
Poultry Science 96 (8) : 2820–2830 (August 2017)
Materia
Gallina Ponedora
Enfermedades de los Animales
Salmonella
Técnicas de Cultivo
PCR
Layer Chickens
Animal Diseases
Culture Techniques
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/6472
Acceder
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oai_identifier_str oai:localhost:20.500.12123/6472
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methodsSoria, Maria CeciliaSoria, MarioBueno, Dante JavierGodano, Eduardo IgnacioGómez, S.C.ViaButron, I.A.Padin, V.M.Rogé, Ariel DiegoGallina PonedoraEnfermedades de los AnimalesSalmonellaTécnicas de CultivoPCRLayer ChickensAnimal DiseasesCulture TechniquesThe performance of detection methods (culture methods and polymerase chain reaction assay) and plating media used in the same type of samples were determined as well as the specificity of PCR primers to detected Salmonella spp. contamination in layer hen farms. Also, the association of farm characteristics with Salmonella presence was evaluated. Environmental samples (feces, feed, drinking water, air, boot-swabs) and eggs were taken from 40 layer hen houses. Salmonella spp. was most detected in boot-swabs taken around the houses (30% and 35% by isolation and PCR, respectively) follow by fecal samples (15.2% and 13.6% by isolation and PCR, respectively). Eggs, drinking water, and air samples were negative for Salmonella detection. Salmonella Schwarzengrund and S. Enteritidis were the most isolated serotypes. For plating media, relative specificity was 1, and the relative sensitivity was greater for EF-18 agar than XLDT agar in feed and fecal samples. However, relative sensitivity was greater in XLDT agar than EF-18 agar for boot-swab samples. Agreement was between fair to good depending on the sample, and it was good between isolation and PCR (feces and boot-swabs), without agreement for feed samples. Salmonella spp. PCR was positive for all strains, while S. Typhimurium PCR was negative. Salmonella Enteritidis PCR used was not specific. Based in the multiple logistic regression analyses, categorization by counties was significant for Salmonella spp. presence (P-value = 0.010). This study shows the importance of considering different types of samples, plating media and detection methods during a Salmonella spp. monitoring study. In addition, it is important to incorporate the sampling of floors around the layer hen houses to learn if biosecurity measures should be strengthened to minimize the entry and spread of Salmonella in the houses. Also, the performance of some PCR methods and S. Enteritidis PCR should be improved, and biosecurity measures in hen farms must be reinforced in the region of more concentrated layer hen houses to reduce the probability of Salmonella spp. presence.EEA Concepción del UruguayFil: Soria, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina.Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Godano, E.I. Tecnovo S.A.; ArgentinaFil: Gómez, S.C. Fundación ArgenINTA, Paraná; Argentina. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología, Sede Concepción del Uruguay; ArgentinaFil: ViaButron, I.A.. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina.Fil: Padin, V.M. Instituto Nacional de Producción de Biológicos (INPB) - ANLIS “Dr Carlos G. Malbrán". Servicio de Antígenos y Antisueros; ArgentinaFil: Rogé, Ariel D. Instituto Nacional de Producción de Biológicos (INPB) - ANLIS “Dr Carlos G. Malbrán". Servicio de Antígenos y Antisueros; ArgentinaOxford Academic Press2019-12-09T12:24:49Z2019-12-09T12:24:49Z2017-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/6472https://academic.oup.com/ps/article/96/8/2820/30968940032-57911525-3171https://doi.org/10.3382/ps/pex053Poultry Science 96 (8) : 2820–2830 (August 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:29:42Zoai:localhost:20.500.12123/6472instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:42.848INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods
title Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods
spellingShingle Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods
Soria, Maria Cecilia
Gallina Ponedora
Enfermedades de los Animales
Salmonella
Técnicas de Cultivo
PCR
Layer Chickens
Animal Diseases
Culture Techniques
title_short Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods
title_full Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods
title_fullStr Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods
title_full_unstemmed Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods
title_sort Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods
dc.creator.none.fl_str_mv Soria, Maria Cecilia
Soria, Mario
Bueno, Dante Javier
Godano, Eduardo Ignacio
Gómez, S.C.
ViaButron, I.A.
Padin, V.M.
Rogé, Ariel Diego
author Soria, Maria Cecilia
author_facet Soria, Maria Cecilia
Soria, Mario
Bueno, Dante Javier
Godano, Eduardo Ignacio
Gómez, S.C.
ViaButron, I.A.
Padin, V.M.
Rogé, Ariel Diego
author_role author
author2 Soria, Mario
Bueno, Dante Javier
Godano, Eduardo Ignacio
Gómez, S.C.
ViaButron, I.A.
Padin, V.M.
Rogé, Ariel Diego
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Gallina Ponedora
Enfermedades de los Animales
Salmonella
Técnicas de Cultivo
PCR
Layer Chickens
Animal Diseases
Culture Techniques
topic Gallina Ponedora
Enfermedades de los Animales
Salmonella
Técnicas de Cultivo
PCR
Layer Chickens
Animal Diseases
Culture Techniques
dc.description.none.fl_txt_mv The performance of detection methods (culture methods and polymerase chain reaction assay) and plating media used in the same type of samples were determined as well as the specificity of PCR primers to detected Salmonella spp. contamination in layer hen farms. Also, the association of farm characteristics with Salmonella presence was evaluated. Environmental samples (feces, feed, drinking water, air, boot-swabs) and eggs were taken from 40 layer hen houses. Salmonella spp. was most detected in boot-swabs taken around the houses (30% and 35% by isolation and PCR, respectively) follow by fecal samples (15.2% and 13.6% by isolation and PCR, respectively). Eggs, drinking water, and air samples were negative for Salmonella detection. Salmonella Schwarzengrund and S. Enteritidis were the most isolated serotypes. For plating media, relative specificity was 1, and the relative sensitivity was greater for EF-18 agar than XLDT agar in feed and fecal samples. However, relative sensitivity was greater in XLDT agar than EF-18 agar for boot-swab samples. Agreement was between fair to good depending on the sample, and it was good between isolation and PCR (feces and boot-swabs), without agreement for feed samples. Salmonella spp. PCR was positive for all strains, while S. Typhimurium PCR was negative. Salmonella Enteritidis PCR used was not specific. Based in the multiple logistic regression analyses, categorization by counties was significant for Salmonella spp. presence (P-value = 0.010). This study shows the importance of considering different types of samples, plating media and detection methods during a Salmonella spp. monitoring study. In addition, it is important to incorporate the sampling of floors around the layer hen houses to learn if biosecurity measures should be strengthened to minimize the entry and spread of Salmonella in the houses. Also, the performance of some PCR methods and S. Enteritidis PCR should be improved, and biosecurity measures in hen farms must be reinforced in the region of more concentrated layer hen houses to reduce the probability of Salmonella spp. presence.
EEA Concepción del Uruguay
Fil: Soria, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina.
Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Godano, E.I. Tecnovo S.A.; Argentina
Fil: Gómez, S.C. Fundación ArgenINTA, Paraná; Argentina. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología, Sede Concepción del Uruguay; Argentina
Fil: ViaButron, I.A.. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina.
Fil: Padin, V.M. Instituto Nacional de Producción de Biológicos (INPB) - ANLIS “Dr Carlos G. Malbrán". Servicio de Antígenos y Antisueros; Argentina
Fil: Rogé, Ariel D. Instituto Nacional de Producción de Biológicos (INPB) - ANLIS “Dr Carlos G. Malbrán". Servicio de Antígenos y Antisueros; Argentina
description The performance of detection methods (culture methods and polymerase chain reaction assay) and plating media used in the same type of samples were determined as well as the specificity of PCR primers to detected Salmonella spp. contamination in layer hen farms. Also, the association of farm characteristics with Salmonella presence was evaluated. Environmental samples (feces, feed, drinking water, air, boot-swabs) and eggs were taken from 40 layer hen houses. Salmonella spp. was most detected in boot-swabs taken around the houses (30% and 35% by isolation and PCR, respectively) follow by fecal samples (15.2% and 13.6% by isolation and PCR, respectively). Eggs, drinking water, and air samples were negative for Salmonella detection. Salmonella Schwarzengrund and S. Enteritidis were the most isolated serotypes. For plating media, relative specificity was 1, and the relative sensitivity was greater for EF-18 agar than XLDT agar in feed and fecal samples. However, relative sensitivity was greater in XLDT agar than EF-18 agar for boot-swab samples. Agreement was between fair to good depending on the sample, and it was good between isolation and PCR (feces and boot-swabs), without agreement for feed samples. Salmonella spp. PCR was positive for all strains, while S. Typhimurium PCR was negative. Salmonella Enteritidis PCR used was not specific. Based in the multiple logistic regression analyses, categorization by counties was significant for Salmonella spp. presence (P-value = 0.010). This study shows the importance of considering different types of samples, plating media and detection methods during a Salmonella spp. monitoring study. In addition, it is important to incorporate the sampling of floors around the layer hen houses to learn if biosecurity measures should be strengthened to minimize the entry and spread of Salmonella in the houses. Also, the performance of some PCR methods and S. Enteritidis PCR should be improved, and biosecurity measures in hen farms must be reinforced in the region of more concentrated layer hen houses to reduce the probability of Salmonella spp. presence.
publishDate 2017
dc.date.none.fl_str_mv 2017-08
2019-12-09T12:24:49Z
2019-12-09T12:24:49Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/6472
https://academic.oup.com/ps/article/96/8/2820/3096894
0032-5791
1525-3171
https://doi.org/10.3382/ps/pex053
url http://hdl.handle.net/20.500.12123/6472
https://academic.oup.com/ps/article/96/8/2820/3096894
https://doi.org/10.3382/ps/pex053
identifier_str_mv 0032-5791
1525-3171
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford Academic Press
publisher.none.fl_str_mv Oxford Academic Press
dc.source.none.fl_str_mv Poultry Science 96 (8) : 2820–2830 (August 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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