Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods
- Autores
- Soria, Maria Cecilia; Soria, Mario; Bueno, Dante Javier; Godano, Eduardo Ignacio; Gómez, S.C.; ViaButron, I.A.; Padin, V.M.; Rogé, Ariel Diego
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The performance of detection methods (culture methods and polymerase chain reaction assay) and plating media used in the same type of samples were determined as well as the specificity of PCR primers to detected Salmonella spp. contamination in layer hen farms. Also, the association of farm characteristics with Salmonella presence was evaluated. Environmental samples (feces, feed, drinking water, air, boot-swabs) and eggs were taken from 40 layer hen houses. Salmonella spp. was most detected in boot-swabs taken around the houses (30% and 35% by isolation and PCR, respectively) follow by fecal samples (15.2% and 13.6% by isolation and PCR, respectively). Eggs, drinking water, and air samples were negative for Salmonella detection. Salmonella Schwarzengrund and S. Enteritidis were the most isolated serotypes. For plating media, relative specificity was 1, and the relative sensitivity was greater for EF-18 agar than XLDT agar in feed and fecal samples. However, relative sensitivity was greater in XLDT agar than EF-18 agar for boot-swab samples. Agreement was between fair to good depending on the sample, and it was good between isolation and PCR (feces and boot-swabs), without agreement for feed samples. Salmonella spp. PCR was positive for all strains, while S. Typhimurium PCR was negative. Salmonella Enteritidis PCR used was not specific. Based in the multiple logistic regression analyses, categorization by counties was significant for Salmonella spp. presence (P-value = 0.010). This study shows the importance of considering different types of samples, plating media and detection methods during a Salmonella spp. monitoring study. In addition, it is important to incorporate the sampling of floors around the layer hen houses to learn if biosecurity measures should be strengthened to minimize the entry and spread of Salmonella in the houses. Also, the performance of some PCR methods and S. Enteritidis PCR should be improved, and biosecurity measures in hen farms must be reinforced in the region of more concentrated layer hen houses to reduce the probability of Salmonella spp. presence.
EEA Concepción del Uruguay
Fil: Soria, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina.
Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Godano, E.I. Tecnovo S.A.; Argentina
Fil: Gómez, S.C. Fundación ArgenINTA, Paraná; Argentina. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología, Sede Concepción del Uruguay; Argentina
Fil: ViaButron, I.A.. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina.
Fil: Padin, V.M. Instituto Nacional de Producción de Biológicos (INPB) - ANLIS “Dr Carlos G. Malbrán". Servicio de Antígenos y Antisueros; Argentina
Fil: Rogé, Ariel D. Instituto Nacional de Producción de Biológicos (INPB) - ANLIS “Dr Carlos G. Malbrán". Servicio de Antígenos y Antisueros; Argentina - Fuente
- Poultry Science 96 (8) : 2820–2830 (August 2017)
- Materia
-
Gallina Ponedora
Enfermedades de los Animales
Salmonella
Técnicas de Cultivo
PCR
Layer Chickens
Animal Diseases
Culture Techniques - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/6472
Ver los metadatos del registro completo
id |
INTADig_f173063a91f0c9c07c4575e58dfe86c6 |
---|---|
oai_identifier_str |
oai:localhost:20.500.12123/6472 |
network_acronym_str |
INTADig |
repository_id_str |
l |
network_name_str |
INTA Digital (INTA) |
spelling |
Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methodsSoria, Maria CeciliaSoria, MarioBueno, Dante JavierGodano, Eduardo IgnacioGómez, S.C.ViaButron, I.A.Padin, V.M.Rogé, Ariel DiegoGallina PonedoraEnfermedades de los AnimalesSalmonellaTécnicas de CultivoPCRLayer ChickensAnimal DiseasesCulture TechniquesThe performance of detection methods (culture methods and polymerase chain reaction assay) and plating media used in the same type of samples were determined as well as the specificity of PCR primers to detected Salmonella spp. contamination in layer hen farms. Also, the association of farm characteristics with Salmonella presence was evaluated. Environmental samples (feces, feed, drinking water, air, boot-swabs) and eggs were taken from 40 layer hen houses. Salmonella spp. was most detected in boot-swabs taken around the houses (30% and 35% by isolation and PCR, respectively) follow by fecal samples (15.2% and 13.6% by isolation and PCR, respectively). Eggs, drinking water, and air samples were negative for Salmonella detection. Salmonella Schwarzengrund and S. Enteritidis were the most isolated serotypes. For plating media, relative specificity was 1, and the relative sensitivity was greater for EF-18 agar than XLDT agar in feed and fecal samples. However, relative sensitivity was greater in XLDT agar than EF-18 agar for boot-swab samples. Agreement was between fair to good depending on the sample, and it was good between isolation and PCR (feces and boot-swabs), without agreement for feed samples. Salmonella spp. PCR was positive for all strains, while S. Typhimurium PCR was negative. Salmonella Enteritidis PCR used was not specific. Based in the multiple logistic regression analyses, categorization by counties was significant for Salmonella spp. presence (P-value = 0.010). This study shows the importance of considering different types of samples, plating media and detection methods during a Salmonella spp. monitoring study. In addition, it is important to incorporate the sampling of floors around the layer hen houses to learn if biosecurity measures should be strengthened to minimize the entry and spread of Salmonella in the houses. Also, the performance of some PCR methods and S. Enteritidis PCR should be improved, and biosecurity measures in hen farms must be reinforced in the region of more concentrated layer hen houses to reduce the probability of Salmonella spp. presence.EEA Concepción del UruguayFil: Soria, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina.Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Godano, E.I. Tecnovo S.A.; ArgentinaFil: Gómez, S.C. Fundación ArgenINTA, Paraná; Argentina. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología, Sede Concepción del Uruguay; ArgentinaFil: ViaButron, I.A.. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina.Fil: Padin, V.M. Instituto Nacional de Producción de Biológicos (INPB) - ANLIS “Dr Carlos G. Malbrán". Servicio de Antígenos y Antisueros; ArgentinaFil: Rogé, Ariel D. Instituto Nacional de Producción de Biológicos (INPB) - ANLIS “Dr Carlos G. Malbrán". Servicio de Antígenos y Antisueros; ArgentinaOxford Academic Press2019-12-09T12:24:49Z2019-12-09T12:24:49Z2017-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/6472https://academic.oup.com/ps/article/96/8/2820/30968940032-57911525-3171https://doi.org/10.3382/ps/pex053Poultry Science 96 (8) : 2820–2830 (August 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:50Zoai:localhost:20.500.12123/6472instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:50.854INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods |
title |
Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods |
spellingShingle |
Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods Soria, Maria Cecilia Gallina Ponedora Enfermedades de los Animales Salmonella Técnicas de Cultivo PCR Layer Chickens Animal Diseases Culture Techniques |
title_short |
Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods |
title_full |
Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods |
title_fullStr |
Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods |
title_full_unstemmed |
Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods |
title_sort |
Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods |
dc.creator.none.fl_str_mv |
Soria, Maria Cecilia Soria, Mario Bueno, Dante Javier Godano, Eduardo Ignacio Gómez, S.C. ViaButron, I.A. Padin, V.M. Rogé, Ariel Diego |
author |
Soria, Maria Cecilia |
author_facet |
Soria, Maria Cecilia Soria, Mario Bueno, Dante Javier Godano, Eduardo Ignacio Gómez, S.C. ViaButron, I.A. Padin, V.M. Rogé, Ariel Diego |
author_role |
author |
author2 |
Soria, Mario Bueno, Dante Javier Godano, Eduardo Ignacio Gómez, S.C. ViaButron, I.A. Padin, V.M. Rogé, Ariel Diego |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
Gallina Ponedora Enfermedades de los Animales Salmonella Técnicas de Cultivo PCR Layer Chickens Animal Diseases Culture Techniques |
topic |
Gallina Ponedora Enfermedades de los Animales Salmonella Técnicas de Cultivo PCR Layer Chickens Animal Diseases Culture Techniques |
dc.description.none.fl_txt_mv |
The performance of detection methods (culture methods and polymerase chain reaction assay) and plating media used in the same type of samples were determined as well as the specificity of PCR primers to detected Salmonella spp. contamination in layer hen farms. Also, the association of farm characteristics with Salmonella presence was evaluated. Environmental samples (feces, feed, drinking water, air, boot-swabs) and eggs were taken from 40 layer hen houses. Salmonella spp. was most detected in boot-swabs taken around the houses (30% and 35% by isolation and PCR, respectively) follow by fecal samples (15.2% and 13.6% by isolation and PCR, respectively). Eggs, drinking water, and air samples were negative for Salmonella detection. Salmonella Schwarzengrund and S. Enteritidis were the most isolated serotypes. For plating media, relative specificity was 1, and the relative sensitivity was greater for EF-18 agar than XLDT agar in feed and fecal samples. However, relative sensitivity was greater in XLDT agar than EF-18 agar for boot-swab samples. Agreement was between fair to good depending on the sample, and it was good between isolation and PCR (feces and boot-swabs), without agreement for feed samples. Salmonella spp. PCR was positive for all strains, while S. Typhimurium PCR was negative. Salmonella Enteritidis PCR used was not specific. Based in the multiple logistic regression analyses, categorization by counties was significant for Salmonella spp. presence (P-value = 0.010). This study shows the importance of considering different types of samples, plating media and detection methods during a Salmonella spp. monitoring study. In addition, it is important to incorporate the sampling of floors around the layer hen houses to learn if biosecurity measures should be strengthened to minimize the entry and spread of Salmonella in the houses. Also, the performance of some PCR methods and S. Enteritidis PCR should be improved, and biosecurity measures in hen farms must be reinforced in the region of more concentrated layer hen houses to reduce the probability of Salmonella spp. presence. EEA Concepción del Uruguay Fil: Soria, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Godano, E.I. Tecnovo S.A.; Argentina Fil: Gómez, S.C. Fundación ArgenINTA, Paraná; Argentina. Universidad Autónoma de Entre Ríos. Facultad de Ciencia y Tecnología, Sede Concepción del Uruguay; Argentina Fil: ViaButron, I.A.. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Fil: Padin, V.M. Instituto Nacional de Producción de Biológicos (INPB) - ANLIS “Dr Carlos G. Malbrán". Servicio de Antígenos y Antisueros; Argentina Fil: Rogé, Ariel D. Instituto Nacional de Producción de Biológicos (INPB) - ANLIS “Dr Carlos G. Malbrán". Servicio de Antígenos y Antisueros; Argentina |
description |
The performance of detection methods (culture methods and polymerase chain reaction assay) and plating media used in the same type of samples were determined as well as the specificity of PCR primers to detected Salmonella spp. contamination in layer hen farms. Also, the association of farm characteristics with Salmonella presence was evaluated. Environmental samples (feces, feed, drinking water, air, boot-swabs) and eggs were taken from 40 layer hen houses. Salmonella spp. was most detected in boot-swabs taken around the houses (30% and 35% by isolation and PCR, respectively) follow by fecal samples (15.2% and 13.6% by isolation and PCR, respectively). Eggs, drinking water, and air samples were negative for Salmonella detection. Salmonella Schwarzengrund and S. Enteritidis were the most isolated serotypes. For plating media, relative specificity was 1, and the relative sensitivity was greater for EF-18 agar than XLDT agar in feed and fecal samples. However, relative sensitivity was greater in XLDT agar than EF-18 agar for boot-swab samples. Agreement was between fair to good depending on the sample, and it was good between isolation and PCR (feces and boot-swabs), without agreement for feed samples. Salmonella spp. PCR was positive for all strains, while S. Typhimurium PCR was negative. Salmonella Enteritidis PCR used was not specific. Based in the multiple logistic regression analyses, categorization by counties was significant for Salmonella spp. presence (P-value = 0.010). This study shows the importance of considering different types of samples, plating media and detection methods during a Salmonella spp. monitoring study. In addition, it is important to incorporate the sampling of floors around the layer hen houses to learn if biosecurity measures should be strengthened to minimize the entry and spread of Salmonella in the houses. Also, the performance of some PCR methods and S. Enteritidis PCR should be improved, and biosecurity measures in hen farms must be reinforced in the region of more concentrated layer hen houses to reduce the probability of Salmonella spp. presence. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-08 2019-12-09T12:24:49Z 2019-12-09T12:24:49Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/6472 https://academic.oup.com/ps/article/96/8/2820/3096894 0032-5791 1525-3171 https://doi.org/10.3382/ps/pex053 |
url |
http://hdl.handle.net/20.500.12123/6472 https://academic.oup.com/ps/article/96/8/2820/3096894 https://doi.org/10.3382/ps/pex053 |
identifier_str_mv |
0032-5791 1525-3171 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Oxford Academic Press |
publisher.none.fl_str_mv |
Oxford Academic Press |
dc.source.none.fl_str_mv |
Poultry Science 96 (8) : 2820–2830 (August 2017) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
_version_ |
1844619140436852736 |
score |
12.559606 |