Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods
- Autores
- Soria, M C; Soria, Marcelo; Bueno, Dante J; Godano, E I; Gómez, S C; ViaButron, I A; Padin, Valeria; Rogé, Ariel
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fil: Soria, M C. Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260, Concepción del Uruguay, Entre Ríos; Argentina.
Fil: Soria, Marcelo. Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260, Concepción del Uruguay, Entre Ríos; Argentina.
Fil: Bueno, Dante J. Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260, Concepción del Uruguay, Entre Ríos; Argentina.
Fil: Godano, E I. Tecnovo S.A., Parque Industrial C.C. 17, 3116, Crespo, Entre Ríos; Argentina.
Fil: Gómez, S C. Fundación ArgenINTA, Ruta 11, Km 12.5, 3101, Oro Verde, Paraná, Entre Ríos; Argentina.
Fil: ViaButron, I A Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260, Concepción del Uruguay, Entre Ríos; Argentina.
Fil: Padin, Valeria. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio antígenos y antisueros; Argentina.
Fil: Rogé, Ariel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio antígenos y antisueros; Argentina.
The performance of detection methods (culture methods and polymerase chain reaction assay) and plating media used in the same type of samples were determined as well as the specificity of PCR primers to detected Salmonella spp. contamination in layer hen farms. Also, the association of farm characteristics with Salmonella presence was evaluated. Environmental samples (feces, feed, drinking water, air, boot-swabs) and eggs were taken from 40 layer hen houses. Salmonella spp. was most detected in boot-swabs taken around the houses (30% and 35% by isolation and PCR, respectively) follow by fecal samples (15.2% and 13.6% by isolation and PCR, respectively). Eggs, drinking water, and air samples were negative for Salmonella detection. Salmonella Schwarzengrund and S. Enteritidis were the most isolated serotypes. For plating media, relative specificity was 1, and the relative sensitivity was greater for EF-18 agar than XLDT agar in feed and fecal samples. However, relative sensitivity was greater in XLDT agar than EF-18 agar for boot-swab samples. Agreement was between fair to good depending on the sample, and it was good between isolation and PCR (feces and boot-swabs), without agreement for feed samples. Salmonella spp. PCR was positive for all strains, while S. Typhimurium PCR was negative. Salmonella Enteritidis PCR used was not specific. Based in the multiple logistic regression analyses, categorization by counties was significant for Salmonella spp. presence (P-value = 0.010). This study shows the importance of considering different types of samples, plating media and detection methods during a Salmonella spp. monitoring study. In addition, it is important to incorporate the sampling of floors around the layer hen houses to learn if biosecurity measures should be strengthened to minimize the entry and spread of Salmonella in the houses. Also, the performance of some PCR methods and S. Enteritidis PCR should be improved, and biosecurity measures in hen farms must be reinforced in the region of more concentrated layer hen houses to reduce the probability of Salmonella spp. presence. - Fuente
- Poultry Science 2017; 96(8):2820-2830.
- Materia
- Salmonella
- Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- Repositorio
- Institución
- Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
- OAI Identificador
- oai:sgc.anlis.gob.ar:123456789/2156
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Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methodsSoria, M CSoria, MarceloBueno, Dante JGodano, E IGómez, S CViaButron, I APadin, ValeriaRogé, ArielSalmonellaFil: Soria, M C. Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260, Concepción del Uruguay, Entre Ríos; Argentina.Fil: Soria, Marcelo. Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260, Concepción del Uruguay, Entre Ríos; Argentina.Fil: Bueno, Dante J. Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260, Concepción del Uruguay, Entre Ríos; Argentina.Fil: Godano, E I. Tecnovo S.A., Parque Industrial C.C. 17, 3116, Crespo, Entre Ríos; Argentina.Fil: Gómez, S C. Fundación ArgenINTA, Ruta 11, Km 12.5, 3101, Oro Verde, Paraná, Entre Ríos; Argentina.Fil: ViaButron, I A Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260, Concepción del Uruguay, Entre Ríos; Argentina.Fil: Padin, Valeria. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio antígenos y antisueros; Argentina.Fil: Rogé, Ariel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio antígenos y antisueros; Argentina.The performance of detection methods (culture methods and polymerase chain reaction assay) and plating media used in the same type of samples were determined as well as the specificity of PCR primers to detected Salmonella spp. contamination in layer hen farms. Also, the association of farm characteristics with Salmonella presence was evaluated. Environmental samples (feces, feed, drinking water, air, boot-swabs) and eggs were taken from 40 layer hen houses. Salmonella spp. was most detected in boot-swabs taken around the houses (30% and 35% by isolation and PCR, respectively) follow by fecal samples (15.2% and 13.6% by isolation and PCR, respectively). Eggs, drinking water, and air samples were negative for Salmonella detection. Salmonella Schwarzengrund and S. Enteritidis were the most isolated serotypes. For plating media, relative specificity was 1, and the relative sensitivity was greater for EF-18 agar than XLDT agar in feed and fecal samples. However, relative sensitivity was greater in XLDT agar than EF-18 agar for boot-swab samples. Agreement was between fair to good depending on the sample, and it was good between isolation and PCR (feces and boot-swabs), without agreement for feed samples. Salmonella spp. PCR was positive for all strains, while S. Typhimurium PCR was negative. Salmonella Enteritidis PCR used was not specific. Based in the multiple logistic regression analyses, categorization by counties was significant for Salmonella spp. presence (P-value = 0.010). This study shows the importance of considering different types of samples, plating media and detection methods during a Salmonella spp. monitoring study. In addition, it is important to incorporate the sampling of floors around the layer hen houses to learn if biosecurity measures should be strengthened to minimize the entry and spread of Salmonella in the houses. Also, the performance of some PCR methods and S. Enteritidis PCR should be improved, and biosecurity measures in hen farms must be reinforced in the region of more concentrated layer hen houses to reduce the probability of Salmonella spp. presence.Elsevier2017-08-01info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1525-3171https://www.sciencedirect.com/science/article/pii/S0032579119314816?via%3Dihubhttp://sgc.anlis.gob.ar/handle/123456789/215610.3382/ps/pex053Poultry Science 2017; 96(8):2820-2830.reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISPoultry scienceenginfo:eu-repo/semantics/openAccess2025-09-29T14:30:41Zoai:sgc.anlis.gob.ar:123456789/2156Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-29 14:30:41.796Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false |
dc.title.none.fl_str_mv |
Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods |
title |
Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods |
spellingShingle |
Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods Soria, M C Salmonella |
title_short |
Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods |
title_full |
Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods |
title_fullStr |
Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods |
title_full_unstemmed |
Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods |
title_sort |
Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods |
dc.creator.none.fl_str_mv |
Soria, M C Soria, Marcelo Bueno, Dante J Godano, E I Gómez, S C ViaButron, I A Padin, Valeria Rogé, Ariel |
author |
Soria, M C |
author_facet |
Soria, M C Soria, Marcelo Bueno, Dante J Godano, E I Gómez, S C ViaButron, I A Padin, Valeria Rogé, Ariel |
author_role |
author |
author2 |
Soria, Marcelo Bueno, Dante J Godano, E I Gómez, S C ViaButron, I A Padin, Valeria Rogé, Ariel |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
Salmonella |
topic |
Salmonella |
dc.description.none.fl_txt_mv |
Fil: Soria, M C. Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260, Concepción del Uruguay, Entre Ríos; Argentina. Fil: Soria, Marcelo. Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260, Concepción del Uruguay, Entre Ríos; Argentina. Fil: Bueno, Dante J. Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260, Concepción del Uruguay, Entre Ríos; Argentina. Fil: Godano, E I. Tecnovo S.A., Parque Industrial C.C. 17, 3116, Crespo, Entre Ríos; Argentina. Fil: Gómez, S C. Fundación ArgenINTA, Ruta 11, Km 12.5, 3101, Oro Verde, Paraná, Entre Ríos; Argentina. Fil: ViaButron, I A Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260, Concepción del Uruguay, Entre Ríos; Argentina. Fil: Padin, Valeria. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio antígenos y antisueros; Argentina. Fil: Rogé, Ariel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio antígenos y antisueros; Argentina. The performance of detection methods (culture methods and polymerase chain reaction assay) and plating media used in the same type of samples were determined as well as the specificity of PCR primers to detected Salmonella spp. contamination in layer hen farms. Also, the association of farm characteristics with Salmonella presence was evaluated. Environmental samples (feces, feed, drinking water, air, boot-swabs) and eggs were taken from 40 layer hen houses. Salmonella spp. was most detected in boot-swabs taken around the houses (30% and 35% by isolation and PCR, respectively) follow by fecal samples (15.2% and 13.6% by isolation and PCR, respectively). Eggs, drinking water, and air samples were negative for Salmonella detection. Salmonella Schwarzengrund and S. Enteritidis were the most isolated serotypes. For plating media, relative specificity was 1, and the relative sensitivity was greater for EF-18 agar than XLDT agar in feed and fecal samples. However, relative sensitivity was greater in XLDT agar than EF-18 agar for boot-swab samples. Agreement was between fair to good depending on the sample, and it was good between isolation and PCR (feces and boot-swabs), without agreement for feed samples. Salmonella spp. PCR was positive for all strains, while S. Typhimurium PCR was negative. Salmonella Enteritidis PCR used was not specific. Based in the multiple logistic regression analyses, categorization by counties was significant for Salmonella spp. presence (P-value = 0.010). This study shows the importance of considering different types of samples, plating media and detection methods during a Salmonella spp. monitoring study. In addition, it is important to incorporate the sampling of floors around the layer hen houses to learn if biosecurity measures should be strengthened to minimize the entry and spread of Salmonella in the houses. Also, the performance of some PCR methods and S. Enteritidis PCR should be improved, and biosecurity measures in hen farms must be reinforced in the region of more concentrated layer hen houses to reduce the probability of Salmonella spp. presence. |
description |
Fil: Soria, M C. Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260, Concepción del Uruguay, Entre Ríos; Argentina. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-08-01 |
dc.type.none.fl_str_mv |
info:ar-repo/semantics/articulo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
1525-3171 https://www.sciencedirect.com/science/article/pii/S0032579119314816?via%3Dihub http://sgc.anlis.gob.ar/handle/123456789/2156 10.3382/ps/pex053 |
identifier_str_mv |
1525-3171 10.3382/ps/pex053 |
url |
https://www.sciencedirect.com/science/article/pii/S0032579119314816?via%3Dihub http://sgc.anlis.gob.ar/handle/123456789/2156 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Poultry science |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
Poultry Science 2017; 96(8):2820-2830. reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" instacron:ANLIS |
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Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
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Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
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Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" |
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ANLIS |
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ANLIS |
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Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" |
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biblioteca@anlis.gov.ar |
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12.559606 |