Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods

Autores
Soria, M C; Soria, Marcelo; Bueno, Dante J; Godano, E I; Gómez, S C; ViaButron, I A; Padin, Valeria; Rogé, Ariel
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Soria, M C. Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260, Concepción del Uruguay, Entre Ríos; Argentina.
Fil: Soria, Marcelo. Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260, Concepción del Uruguay, Entre Ríos; Argentina.
Fil: Bueno, Dante J. Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260, Concepción del Uruguay, Entre Ríos; Argentina.
Fil: Godano, E I. Tecnovo S.A., Parque Industrial C.C. 17, 3116, Crespo, Entre Ríos; Argentina.
Fil: Gómez, S C. Fundación ArgenINTA, Ruta 11, Km 12.5, 3101, Oro Verde, Paraná, Entre Ríos; Argentina.
Fil: ViaButron, I A Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260, Concepción del Uruguay, Entre Ríos; Argentina.
Fil: Padin, Valeria. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio antígenos y antisueros; Argentina.
Fil: Rogé, Ariel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio antígenos y antisueros; Argentina.
The performance of detection methods (culture methods and polymerase chain reaction assay) and plating media used in the same type of samples were determined as well as the specificity of PCR primers to detected Salmonella spp. contamination in layer hen farms. Also, the association of farm characteristics with Salmonella presence was evaluated. Environmental samples (feces, feed, drinking water, air, boot-swabs) and eggs were taken from 40 layer hen houses. Salmonella spp. was most detected in boot-swabs taken around the houses (30% and 35% by isolation and PCR, respectively) follow by fecal samples (15.2% and 13.6% by isolation and PCR, respectively). Eggs, drinking water, and air samples were negative for Salmonella detection. Salmonella Schwarzengrund and S. Enteritidis were the most isolated serotypes. For plating media, relative specificity was 1, and the relative sensitivity was greater for EF-18 agar than XLDT agar in feed and fecal samples. However, relative sensitivity was greater in XLDT agar than EF-18 agar for boot-swab samples. Agreement was between fair to good depending on the sample, and it was good between isolation and PCR (feces and boot-swabs), without agreement for feed samples. Salmonella spp. PCR was positive for all strains, while S. Typhimurium PCR was negative. Salmonella Enteritidis PCR used was not specific. Based in the multiple logistic regression analyses, categorization by counties was significant for Salmonella spp. presence (P-value = 0.010). This study shows the importance of considering different types of samples, plating media and detection methods during a Salmonella spp. monitoring study. In addition, it is important to incorporate the sampling of floors around the layer hen houses to learn if biosecurity measures should be strengthened to minimize the entry and spread of Salmonella in the houses. Also, the performance of some PCR methods and S. Enteritidis PCR should be improved, and biosecurity measures in hen farms must be reinforced in the region of more concentrated layer hen houses to reduce the probability of Salmonella spp. presence.
Fuente
Poultry Science 2017; 96(8):2820-2830.
Materia
Salmonella
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:123456789/2156

id SGCANLIS_e9c02afd188a791e3d71d05abe5616a7
oai_identifier_str oai:sgc.anlis.gob.ar:123456789/2156
network_acronym_str SGCANLIS
repository_id_str a
network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methodsSoria, M CSoria, MarceloBueno, Dante JGodano, E IGómez, S CViaButron, I APadin, ValeriaRogé, ArielSalmonellaFil: Soria, M C. Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260, Concepción del Uruguay, Entre Ríos; Argentina.Fil: Soria, Marcelo. Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260, Concepción del Uruguay, Entre Ríos; Argentina.Fil: Bueno, Dante J. Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260, Concepción del Uruguay, Entre Ríos; Argentina.Fil: Godano, E I. Tecnovo S.A., Parque Industrial C.C. 17, 3116, Crespo, Entre Ríos; Argentina.Fil: Gómez, S C. Fundación ArgenINTA, Ruta 11, Km 12.5, 3101, Oro Verde, Paraná, Entre Ríos; Argentina.Fil: ViaButron, I A Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260, Concepción del Uruguay, Entre Ríos; Argentina.Fil: Padin, Valeria. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio antígenos y antisueros; Argentina.Fil: Rogé, Ariel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio antígenos y antisueros; Argentina.The performance of detection methods (culture methods and polymerase chain reaction assay) and plating media used in the same type of samples were determined as well as the specificity of PCR primers to detected Salmonella spp. contamination in layer hen farms. Also, the association of farm characteristics with Salmonella presence was evaluated. Environmental samples (feces, feed, drinking water, air, boot-swabs) and eggs were taken from 40 layer hen houses. Salmonella spp. was most detected in boot-swabs taken around the houses (30% and 35% by isolation and PCR, respectively) follow by fecal samples (15.2% and 13.6% by isolation and PCR, respectively). Eggs, drinking water, and air samples were negative for Salmonella detection. Salmonella Schwarzengrund and S. Enteritidis were the most isolated serotypes. For plating media, relative specificity was 1, and the relative sensitivity was greater for EF-18 agar than XLDT agar in feed and fecal samples. However, relative sensitivity was greater in XLDT agar than EF-18 agar for boot-swab samples. Agreement was between fair to good depending on the sample, and it was good between isolation and PCR (feces and boot-swabs), without agreement for feed samples. Salmonella spp. PCR was positive for all strains, while S. Typhimurium PCR was negative. Salmonella Enteritidis PCR used was not specific. Based in the multiple logistic regression analyses, categorization by counties was significant for Salmonella spp. presence (P-value = 0.010). This study shows the importance of considering different types of samples, plating media and detection methods during a Salmonella spp. monitoring study. In addition, it is important to incorporate the sampling of floors around the layer hen houses to learn if biosecurity measures should be strengthened to minimize the entry and spread of Salmonella in the houses. Also, the performance of some PCR methods and S. Enteritidis PCR should be improved, and biosecurity measures in hen farms must be reinforced in the region of more concentrated layer hen houses to reduce the probability of Salmonella spp. presence.Elsevier2017-08-01info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1525-3171https://www.sciencedirect.com/science/article/pii/S0032579119314816?via%3Dihubhttp://sgc.anlis.gob.ar/handle/123456789/215610.3382/ps/pex053Poultry Science 2017; 96(8):2820-2830.reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISPoultry scienceenginfo:eu-repo/semantics/openAccess2025-09-29T14:30:41Zoai:sgc.anlis.gob.ar:123456789/2156Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-29 14:30:41.796Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods
title Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods
spellingShingle Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods
Soria, M C
Salmonella
title_short Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods
title_full Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods
title_fullStr Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods
title_full_unstemmed Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods
title_sort Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods
dc.creator.none.fl_str_mv Soria, M C
Soria, Marcelo
Bueno, Dante J
Godano, E I
Gómez, S C
ViaButron, I A
Padin, Valeria
Rogé, Ariel
author Soria, M C
author_facet Soria, M C
Soria, Marcelo
Bueno, Dante J
Godano, E I
Gómez, S C
ViaButron, I A
Padin, Valeria
Rogé, Ariel
author_role author
author2 Soria, Marcelo
Bueno, Dante J
Godano, E I
Gómez, S C
ViaButron, I A
Padin, Valeria
Rogé, Ariel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Salmonella
topic Salmonella
dc.description.none.fl_txt_mv Fil: Soria, M C. Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260, Concepción del Uruguay, Entre Ríos; Argentina.
Fil: Soria, Marcelo. Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260, Concepción del Uruguay, Entre Ríos; Argentina.
Fil: Bueno, Dante J. Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260, Concepción del Uruguay, Entre Ríos; Argentina.
Fil: Godano, E I. Tecnovo S.A., Parque Industrial C.C. 17, 3116, Crespo, Entre Ríos; Argentina.
Fil: Gómez, S C. Fundación ArgenINTA, Ruta 11, Km 12.5, 3101, Oro Verde, Paraná, Entre Ríos; Argentina.
Fil: ViaButron, I A Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260, Concepción del Uruguay, Entre Ríos; Argentina.
Fil: Padin, Valeria. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio antígenos y antisueros; Argentina.
Fil: Rogé, Ariel. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos. Servicio antígenos y antisueros; Argentina.
The performance of detection methods (culture methods and polymerase chain reaction assay) and plating media used in the same type of samples were determined as well as the specificity of PCR primers to detected Salmonella spp. contamination in layer hen farms. Also, the association of farm characteristics with Salmonella presence was evaluated. Environmental samples (feces, feed, drinking water, air, boot-swabs) and eggs were taken from 40 layer hen houses. Salmonella spp. was most detected in boot-swabs taken around the houses (30% and 35% by isolation and PCR, respectively) follow by fecal samples (15.2% and 13.6% by isolation and PCR, respectively). Eggs, drinking water, and air samples were negative for Salmonella detection. Salmonella Schwarzengrund and S. Enteritidis were the most isolated serotypes. For plating media, relative specificity was 1, and the relative sensitivity was greater for EF-18 agar than XLDT agar in feed and fecal samples. However, relative sensitivity was greater in XLDT agar than EF-18 agar for boot-swab samples. Agreement was between fair to good depending on the sample, and it was good between isolation and PCR (feces and boot-swabs), without agreement for feed samples. Salmonella spp. PCR was positive for all strains, while S. Typhimurium PCR was negative. Salmonella Enteritidis PCR used was not specific. Based in the multiple logistic regression analyses, categorization by counties was significant for Salmonella spp. presence (P-value = 0.010). This study shows the importance of considering different types of samples, plating media and detection methods during a Salmonella spp. monitoring study. In addition, it is important to incorporate the sampling of floors around the layer hen houses to learn if biosecurity measures should be strengthened to minimize the entry and spread of Salmonella in the houses. Also, the performance of some PCR methods and S. Enteritidis PCR should be improved, and biosecurity measures in hen farms must be reinforced in the region of more concentrated layer hen houses to reduce the probability of Salmonella spp. presence.
description Fil: Soria, M C. Instituto Nacional de Tecnología Agropecuaria EEA Concepción del Uruguay, Ruta Provincial 39 Km 143,5, 3260, Concepción del Uruguay, Entre Ríos; Argentina.
publishDate 2017
dc.date.none.fl_str_mv 2017-08-01
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 1525-3171
https://www.sciencedirect.com/science/article/pii/S0032579119314816?via%3Dihub
http://sgc.anlis.gob.ar/handle/123456789/2156
10.3382/ps/pex053
identifier_str_mv 1525-3171
10.3382/ps/pex053
url https://www.sciencedirect.com/science/article/pii/S0032579119314816?via%3Dihub
http://sgc.anlis.gob.ar/handle/123456789/2156
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Poultry science
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Poultry Science 2017; 96(8):2820-2830.
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
_version_ 1844621859240280064
score 12.559606