Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods

Autores
Soria, María Cecilia; Soria, María A.; Bueno, Dante Javier; Godano, E. I.; Gómez, S.C.; ViaButron, I. A.; Padin, V. M.; Rogé, A. D.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The performance of detection methods (culture methods and polymerase chain reaction assay) and plating media used in the same type of samples were determined as well as the specificity of PCR primers to detected Salmonella spp. contamination in layer hen farms. Also, the association of farm characteristics with Salmonella presence was evaluated. Environmental samples (feces, feed, drinking water, air, bootswabs) and eggs were taken from 40 layer hen houses. Salmonella spp. was most detected in boot-swabs taken around the houses (30% and 35% by isolation and PCR, respectively) follow by fecal samples (15.2% and 13.6% by isolation and PCR, respectively). Eggs, drinking water, and air samples were negative for Salmonella detection. Salmonella Schwarzengrund and S. Enteritidis were the most isolated serotypes. For plating media, relative specificity was 1, and the relative sensitivity was greater for EF-18 agar than XLDT agar in feed and fecal samples. However, relative sensitivity was greater in XLDT agar than EF-18 agar for boot-swab samples. Agreement was between fair to good depending on the sample, and it was good between isolation and PCR (feces and boot-swabs), without agreement for feed samples. Salmonella spp. PCR was positive for all strains, while S. Typhimurium PCR was negative. Salmonella Enteritidis PCR used was not specific. Based in the multiple logistic regression analyses, categorization by counties was significant for Salmonella spp. presence (P-value = 0.010). This study shows the importance of considering different types of samples, plating media and detection methods during a Salmonella spp. monitoring study. In addition, it is important to incorporate the sampling of floors around the layer hen houses to learn if biosecurity measures should be strengthened to minimize the entry and spread of Salmonella in the houses. Also, the performance of some PCR methods and S. Enteritidis PCR should be improved, and biosecurity measures in hen farms must be reinforced in the region of more concentrated layer hen houses to reduce the probability of Salmonella spp. presence.
Fil: Soria, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Entre Ríos. Universidad Nacional de Entre Ríos. Centro de Investigaciones y Transferencia de Entre Ríos; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; Argentina
Fil: Soria, María A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; Argentina
Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; Argentina
Fil: Godano, E. I.. Tecnovo S.A.; Argentina
Fil: Gómez, S.C.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; Argentina
Fil: ViaButron, I. A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; Argentina
Fil: Padin, V. M.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Rogé, A. D.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Materia
DETECTION
LAYER HEN FARM
SALMONELLA
SAMPLING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/63598
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/63598
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methodsSoria, María CeciliaSoria, María A.Bueno, Dante JavierGodano, E. I.Gómez, S.C.ViaButron, I. A.Padin, V. M.Rogé, A. D.DETECTIONLAYER HEN FARMSALMONELLASAMPLINGhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The performance of detection methods (culture methods and polymerase chain reaction assay) and plating media used in the same type of samples were determined as well as the specificity of PCR primers to detected Salmonella spp. contamination in layer hen farms. Also, the association of farm characteristics with Salmonella presence was evaluated. Environmental samples (feces, feed, drinking water, air, bootswabs) and eggs were taken from 40 layer hen houses. Salmonella spp. was most detected in boot-swabs taken around the houses (30% and 35% by isolation and PCR, respectively) follow by fecal samples (15.2% and 13.6% by isolation and PCR, respectively). Eggs, drinking water, and air samples were negative for Salmonella detection. Salmonella Schwarzengrund and S. Enteritidis were the most isolated serotypes. For plating media, relative specificity was 1, and the relative sensitivity was greater for EF-18 agar than XLDT agar in feed and fecal samples. However, relative sensitivity was greater in XLDT agar than EF-18 agar for boot-swab samples. Agreement was between fair to good depending on the sample, and it was good between isolation and PCR (feces and boot-swabs), without agreement for feed samples. Salmonella spp. PCR was positive for all strains, while S. Typhimurium PCR was negative. Salmonella Enteritidis PCR used was not specific. Based in the multiple logistic regression analyses, categorization by counties was significant for Salmonella spp. presence (P-value = 0.010). This study shows the importance of considering different types of samples, plating media and detection methods during a Salmonella spp. monitoring study. In addition, it is important to incorporate the sampling of floors around the layer hen houses to learn if biosecurity measures should be strengthened to minimize the entry and spread of Salmonella in the houses. Also, the performance of some PCR methods and S. Enteritidis PCR should be improved, and biosecurity measures in hen farms must be reinforced in the region of more concentrated layer hen houses to reduce the probability of Salmonella spp. presence.Fil: Soria, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Entre Ríos. Universidad Nacional de Entre Ríos. Centro de Investigaciones y Transferencia de Entre Ríos; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; ArgentinaFil: Soria, María A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; ArgentinaFil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; ArgentinaFil: Godano, E. I.. Tecnovo S.A.; ArgentinaFil: Gómez, S.C.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; ArgentinaFil: ViaButron, I. A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; ArgentinaFil: Padin, V. M.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; ArgentinaFil: Rogé, A. D.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; ArgentinaPoultry Science Association2017-08-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/63598Soria, María Cecilia; Soria, María A.; Bueno, Dante Javier; Godano, E. I.; Gómez, S.C.; et al.; Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods; Poultry Science Association; Poultry Science; 96; 8; 31-8-2017; 2820-28300032-5791CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3382/ps/pex053info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/ps/article/96/8/2820/3096894info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:45:12Zoai:ri.conicet.gov.ar:11336/63598instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:45:12.899CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods
title Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods
spellingShingle Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods
Soria, María Cecilia
DETECTION
LAYER HEN FARM
SALMONELLA
SAMPLING
title_short Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods
title_full Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods
title_fullStr Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods
title_full_unstemmed Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods
title_sort Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods
dc.creator.none.fl_str_mv Soria, María Cecilia
Soria, María A.
Bueno, Dante Javier
Godano, E. I.
Gómez, S.C.
ViaButron, I. A.
Padin, V. M.
Rogé, A. D.
author Soria, María Cecilia
author_facet Soria, María Cecilia
Soria, María A.
Bueno, Dante Javier
Godano, E. I.
Gómez, S.C.
ViaButron, I. A.
Padin, V. M.
Rogé, A. D.
author_role author
author2 Soria, María A.
Bueno, Dante Javier
Godano, E. I.
Gómez, S.C.
ViaButron, I. A.
Padin, V. M.
Rogé, A. D.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DETECTION
LAYER HEN FARM
SALMONELLA
SAMPLING
topic DETECTION
LAYER HEN FARM
SALMONELLA
SAMPLING
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The performance of detection methods (culture methods and polymerase chain reaction assay) and plating media used in the same type of samples were determined as well as the specificity of PCR primers to detected Salmonella spp. contamination in layer hen farms. Also, the association of farm characteristics with Salmonella presence was evaluated. Environmental samples (feces, feed, drinking water, air, bootswabs) and eggs were taken from 40 layer hen houses. Salmonella spp. was most detected in boot-swabs taken around the houses (30% and 35% by isolation and PCR, respectively) follow by fecal samples (15.2% and 13.6% by isolation and PCR, respectively). Eggs, drinking water, and air samples were negative for Salmonella detection. Salmonella Schwarzengrund and S. Enteritidis were the most isolated serotypes. For plating media, relative specificity was 1, and the relative sensitivity was greater for EF-18 agar than XLDT agar in feed and fecal samples. However, relative sensitivity was greater in XLDT agar than EF-18 agar for boot-swab samples. Agreement was between fair to good depending on the sample, and it was good between isolation and PCR (feces and boot-swabs), without agreement for feed samples. Salmonella spp. PCR was positive for all strains, while S. Typhimurium PCR was negative. Salmonella Enteritidis PCR used was not specific. Based in the multiple logistic regression analyses, categorization by counties was significant for Salmonella spp. presence (P-value = 0.010). This study shows the importance of considering different types of samples, plating media and detection methods during a Salmonella spp. monitoring study. In addition, it is important to incorporate the sampling of floors around the layer hen houses to learn if biosecurity measures should be strengthened to minimize the entry and spread of Salmonella in the houses. Also, the performance of some PCR methods and S. Enteritidis PCR should be improved, and biosecurity measures in hen farms must be reinforced in the region of more concentrated layer hen houses to reduce the probability of Salmonella spp. presence.
Fil: Soria, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Entre Ríos. Universidad Nacional de Entre Ríos. Centro de Investigaciones y Transferencia de Entre Ríos; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; Argentina
Fil: Soria, María A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; Argentina
Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; Argentina
Fil: Godano, E. I.. Tecnovo S.A.; Argentina
Fil: Gómez, S.C.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; Argentina
Fil: ViaButron, I. A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; Argentina
Fil: Padin, V. M.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Rogé, A. D.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
description The performance of detection methods (culture methods and polymerase chain reaction assay) and plating media used in the same type of samples were determined as well as the specificity of PCR primers to detected Salmonella spp. contamination in layer hen farms. Also, the association of farm characteristics with Salmonella presence was evaluated. Environmental samples (feces, feed, drinking water, air, bootswabs) and eggs were taken from 40 layer hen houses. Salmonella spp. was most detected in boot-swabs taken around the houses (30% and 35% by isolation and PCR, respectively) follow by fecal samples (15.2% and 13.6% by isolation and PCR, respectively). Eggs, drinking water, and air samples were negative for Salmonella detection. Salmonella Schwarzengrund and S. Enteritidis were the most isolated serotypes. For plating media, relative specificity was 1, and the relative sensitivity was greater for EF-18 agar than XLDT agar in feed and fecal samples. However, relative sensitivity was greater in XLDT agar than EF-18 agar for boot-swab samples. Agreement was between fair to good depending on the sample, and it was good between isolation and PCR (feces and boot-swabs), without agreement for feed samples. Salmonella spp. PCR was positive for all strains, while S. Typhimurium PCR was negative. Salmonella Enteritidis PCR used was not specific. Based in the multiple logistic regression analyses, categorization by counties was significant for Salmonella spp. presence (P-value = 0.010). This study shows the importance of considering different types of samples, plating media and detection methods during a Salmonella spp. monitoring study. In addition, it is important to incorporate the sampling of floors around the layer hen houses to learn if biosecurity measures should be strengthened to minimize the entry and spread of Salmonella in the houses. Also, the performance of some PCR methods and S. Enteritidis PCR should be improved, and biosecurity measures in hen farms must be reinforced in the region of more concentrated layer hen houses to reduce the probability of Salmonella spp. presence.
publishDate 2017
dc.date.none.fl_str_mv 2017-08-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/63598
Soria, María Cecilia; Soria, María A.; Bueno, Dante Javier; Godano, E. I.; Gómez, S.C.; et al.; Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods; Poultry Science Association; Poultry Science; 96; 8; 31-8-2017; 2820-2830
0032-5791
CONICET Digital
CONICET
url http://hdl.handle.net/11336/63598
identifier_str_mv Soria, María Cecilia; Soria, María A.; Bueno, Dante Javier; Godano, E. I.; Gómez, S.C.; et al.; Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods; Poultry Science Association; Poultry Science; 96; 8; 31-8-2017; 2820-2830
0032-5791
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3382/ps/pex053
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/ps/article/96/8/2820/3096894
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Poultry Science Association
publisher.none.fl_str_mv Poultry Science Association
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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