Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods
- Autores
- Soria, María Cecilia; Soria, María A.; Bueno, Dante Javier; Godano, E. I.; Gómez, S.C.; ViaButron, I. A.; Padin, V. M.; Rogé, A. D.
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The performance of detection methods (culture methods and polymerase chain reaction assay) and plating media used in the same type of samples were determined as well as the specificity of PCR primers to detected Salmonella spp. contamination in layer hen farms. Also, the association of farm characteristics with Salmonella presence was evaluated. Environmental samples (feces, feed, drinking water, air, bootswabs) and eggs were taken from 40 layer hen houses. Salmonella spp. was most detected in boot-swabs taken around the houses (30% and 35% by isolation and PCR, respectively) follow by fecal samples (15.2% and 13.6% by isolation and PCR, respectively). Eggs, drinking water, and air samples were negative for Salmonella detection. Salmonella Schwarzengrund and S. Enteritidis were the most isolated serotypes. For plating media, relative specificity was 1, and the relative sensitivity was greater for EF-18 agar than XLDT agar in feed and fecal samples. However, relative sensitivity was greater in XLDT agar than EF-18 agar for boot-swab samples. Agreement was between fair to good depending on the sample, and it was good between isolation and PCR (feces and boot-swabs), without agreement for feed samples. Salmonella spp. PCR was positive for all strains, while S. Typhimurium PCR was negative. Salmonella Enteritidis PCR used was not specific. Based in the multiple logistic regression analyses, categorization by counties was significant for Salmonella spp. presence (P-value = 0.010). This study shows the importance of considering different types of samples, plating media and detection methods during a Salmonella spp. monitoring study. In addition, it is important to incorporate the sampling of floors around the layer hen houses to learn if biosecurity measures should be strengthened to minimize the entry and spread of Salmonella in the houses. Also, the performance of some PCR methods and S. Enteritidis PCR should be improved, and biosecurity measures in hen farms must be reinforced in the region of more concentrated layer hen houses to reduce the probability of Salmonella spp. presence.
Fil: Soria, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Entre Ríos. Universidad Nacional de Entre Ríos. Centro de Investigaciones y Transferencia de Entre Ríos; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; Argentina
Fil: Soria, María A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; Argentina
Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; Argentina
Fil: Godano, E. I.. Tecnovo S.A.; Argentina
Fil: Gómez, S.C.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; Argentina
Fil: ViaButron, I. A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; Argentina
Fil: Padin, V. M.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Rogé, A. D.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina - Materia
-
DETECTION
LAYER HEN FARM
SALMONELLA
SAMPLING - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/63598
Ver los metadatos del registro completo
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CONICET Digital (CONICET) |
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Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methodsSoria, María CeciliaSoria, María A.Bueno, Dante JavierGodano, E. I.Gómez, S.C.ViaButron, I. A.Padin, V. M.Rogé, A. D.DETECTIONLAYER HEN FARMSALMONELLASAMPLINGhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The performance of detection methods (culture methods and polymerase chain reaction assay) and plating media used in the same type of samples were determined as well as the specificity of PCR primers to detected Salmonella spp. contamination in layer hen farms. Also, the association of farm characteristics with Salmonella presence was evaluated. Environmental samples (feces, feed, drinking water, air, bootswabs) and eggs were taken from 40 layer hen houses. Salmonella spp. was most detected in boot-swabs taken around the houses (30% and 35% by isolation and PCR, respectively) follow by fecal samples (15.2% and 13.6% by isolation and PCR, respectively). Eggs, drinking water, and air samples were negative for Salmonella detection. Salmonella Schwarzengrund and S. Enteritidis were the most isolated serotypes. For plating media, relative specificity was 1, and the relative sensitivity was greater for EF-18 agar than XLDT agar in feed and fecal samples. However, relative sensitivity was greater in XLDT agar than EF-18 agar for boot-swab samples. Agreement was between fair to good depending on the sample, and it was good between isolation and PCR (feces and boot-swabs), without agreement for feed samples. Salmonella spp. PCR was positive for all strains, while S. Typhimurium PCR was negative. Salmonella Enteritidis PCR used was not specific. Based in the multiple logistic regression analyses, categorization by counties was significant for Salmonella spp. presence (P-value = 0.010). This study shows the importance of considering different types of samples, plating media and detection methods during a Salmonella spp. monitoring study. In addition, it is important to incorporate the sampling of floors around the layer hen houses to learn if biosecurity measures should be strengthened to minimize the entry and spread of Salmonella in the houses. Also, the performance of some PCR methods and S. Enteritidis PCR should be improved, and biosecurity measures in hen farms must be reinforced in the region of more concentrated layer hen houses to reduce the probability of Salmonella spp. presence.Fil: Soria, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Entre Ríos. Universidad Nacional de Entre Ríos. Centro de Investigaciones y Transferencia de Entre Ríos; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; ArgentinaFil: Soria, María A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; ArgentinaFil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; ArgentinaFil: Godano, E. I.. Tecnovo S.A.; ArgentinaFil: Gómez, S.C.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; ArgentinaFil: ViaButron, I. A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; ArgentinaFil: Padin, V. M.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; ArgentinaFil: Rogé, A. D.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; ArgentinaPoultry Science Association2017-08-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/63598Soria, María Cecilia; Soria, María A.; Bueno, Dante Javier; Godano, E. I.; Gómez, S.C.; et al.; Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods; Poultry Science Association; Poultry Science; 96; 8; 31-8-2017; 2820-28300032-5791CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3382/ps/pex053info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/ps/article/96/8/2820/3096894info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:14:56Zoai:ri.conicet.gov.ar:11336/63598instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:14:56.532CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods |
title |
Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods |
spellingShingle |
Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods Soria, María Cecilia DETECTION LAYER HEN FARM SALMONELLA SAMPLING |
title_short |
Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods |
title_full |
Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods |
title_fullStr |
Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods |
title_full_unstemmed |
Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods |
title_sort |
Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods |
dc.creator.none.fl_str_mv |
Soria, María Cecilia Soria, María A. Bueno, Dante Javier Godano, E. I. Gómez, S.C. ViaButron, I. A. Padin, V. M. Rogé, A. D. |
author |
Soria, María Cecilia |
author_facet |
Soria, María Cecilia Soria, María A. Bueno, Dante Javier Godano, E. I. Gómez, S.C. ViaButron, I. A. Padin, V. M. Rogé, A. D. |
author_role |
author |
author2 |
Soria, María A. Bueno, Dante Javier Godano, E. I. Gómez, S.C. ViaButron, I. A. Padin, V. M. Rogé, A. D. |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
DETECTION LAYER HEN FARM SALMONELLA SAMPLING |
topic |
DETECTION LAYER HEN FARM SALMONELLA SAMPLING |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 https://purl.org/becyt/ford/4.3 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
The performance of detection methods (culture methods and polymerase chain reaction assay) and plating media used in the same type of samples were determined as well as the specificity of PCR primers to detected Salmonella spp. contamination in layer hen farms. Also, the association of farm characteristics with Salmonella presence was evaluated. Environmental samples (feces, feed, drinking water, air, bootswabs) and eggs were taken from 40 layer hen houses. Salmonella spp. was most detected in boot-swabs taken around the houses (30% and 35% by isolation and PCR, respectively) follow by fecal samples (15.2% and 13.6% by isolation and PCR, respectively). Eggs, drinking water, and air samples were negative for Salmonella detection. Salmonella Schwarzengrund and S. Enteritidis were the most isolated serotypes. For plating media, relative specificity was 1, and the relative sensitivity was greater for EF-18 agar than XLDT agar in feed and fecal samples. However, relative sensitivity was greater in XLDT agar than EF-18 agar for boot-swab samples. Agreement was between fair to good depending on the sample, and it was good between isolation and PCR (feces and boot-swabs), without agreement for feed samples. Salmonella spp. PCR was positive for all strains, while S. Typhimurium PCR was negative. Salmonella Enteritidis PCR used was not specific. Based in the multiple logistic regression analyses, categorization by counties was significant for Salmonella spp. presence (P-value = 0.010). This study shows the importance of considering different types of samples, plating media and detection methods during a Salmonella spp. monitoring study. In addition, it is important to incorporate the sampling of floors around the layer hen houses to learn if biosecurity measures should be strengthened to minimize the entry and spread of Salmonella in the houses. Also, the performance of some PCR methods and S. Enteritidis PCR should be improved, and biosecurity measures in hen farms must be reinforced in the region of more concentrated layer hen houses to reduce the probability of Salmonella spp. presence. Fil: Soria, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Entre Ríos. Universidad Nacional de Entre Ríos. Centro de Investigaciones y Transferencia de Entre Ríos; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; Argentina Fil: Soria, María A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; Argentina Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; Argentina Fil: Godano, E. I.. Tecnovo S.A.; Argentina Fil: Gómez, S.C.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; Argentina Fil: ViaButron, I. A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios; Argentina Fil: Padin, V. M.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina Fil: Rogé, A. D.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina |
description |
The performance of detection methods (culture methods and polymerase chain reaction assay) and plating media used in the same type of samples were determined as well as the specificity of PCR primers to detected Salmonella spp. contamination in layer hen farms. Also, the association of farm characteristics with Salmonella presence was evaluated. Environmental samples (feces, feed, drinking water, air, bootswabs) and eggs were taken from 40 layer hen houses. Salmonella spp. was most detected in boot-swabs taken around the houses (30% and 35% by isolation and PCR, respectively) follow by fecal samples (15.2% and 13.6% by isolation and PCR, respectively). Eggs, drinking water, and air samples were negative for Salmonella detection. Salmonella Schwarzengrund and S. Enteritidis were the most isolated serotypes. For plating media, relative specificity was 1, and the relative sensitivity was greater for EF-18 agar than XLDT agar in feed and fecal samples. However, relative sensitivity was greater in XLDT agar than EF-18 agar for boot-swab samples. Agreement was between fair to good depending on the sample, and it was good between isolation and PCR (feces and boot-swabs), without agreement for feed samples. Salmonella spp. PCR was positive for all strains, while S. Typhimurium PCR was negative. Salmonella Enteritidis PCR used was not specific. Based in the multiple logistic regression analyses, categorization by counties was significant for Salmonella spp. presence (P-value = 0.010). This study shows the importance of considering different types of samples, plating media and detection methods during a Salmonella spp. monitoring study. In addition, it is important to incorporate the sampling of floors around the layer hen houses to learn if biosecurity measures should be strengthened to minimize the entry and spread of Salmonella in the houses. Also, the performance of some PCR methods and S. Enteritidis PCR should be improved, and biosecurity measures in hen farms must be reinforced in the region of more concentrated layer hen houses to reduce the probability of Salmonella spp. presence. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-08-31 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/63598 Soria, María Cecilia; Soria, María A.; Bueno, Dante Javier; Godano, E. I.; Gómez, S.C.; et al.; Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods; Poultry Science Association; Poultry Science; 96; 8; 31-8-2017; 2820-2830 0032-5791 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/63598 |
identifier_str_mv |
Soria, María Cecilia; Soria, María A.; Bueno, Dante Javier; Godano, E. I.; Gómez, S.C.; et al.; Salmonella spp. contamination in commercial layer hen farms using different types of samples and detection methods; Poultry Science Association; Poultry Science; 96; 8; 31-8-2017; 2820-2830 0032-5791 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.3382/ps/pex053 info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/ps/article/96/8/2820/3096894 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Poultry Science Association |
publisher.none.fl_str_mv |
Poultry Science Association |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614082281340928 |
score |
13.070432 |