Discovery of antigens and cellular mechanisms in the protozoan parasite sarcocystis aucheniae using immunoproteomics

Autores
Wieser, Sara Nathaly; Decker Franco, Cecilia; De Alba, Paloma; Romero, Sandra Raquel; Ferrari, Alejandro; Schnittger, Leonhard; Florin-Christensen, Monica
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sarcocystis aucheniae is a coccidian parasite that produces macroscopic sarcocysts in South American camelid (SAC) muscles and causes a disease known as SAC sarcocystosis. This parasitosis hampers the commercialization of llama and alpaca meat, a vital economic activity in the Andean regions. No control or prevention methods are available, and diagnosis is based on postmortem visual inspection of carcasses. The aim of this study was to identify S. aucheniae B-cell epitopes suitable for the development of diagnostic methods for SAC sarcocystosis. To this end, sarcocyst immunoreactive protein bands were analyzed via mass spectrometry, and proteins in each band were identified in silico by searching in the parasite transcriptome. Five highly antigenic, hydrophilic B-cell epitopes, predicted not to cross-react with antibodies against other coccidia, were selected for future development of peptide-based serological tests. In addition, conserved domains present in the identified proteins allowed us to unravel metabolic pathways and mechanisms active in the parasitic stages present in sarcocysts, including aerobic respiration, antioxidant activity, signal transduction, protein synthesis and processing, and host–pathogen interactions. This study provides novel information on the biology of S. aucheniae, as well as new protein sequences that can be used for the development of diagnostic tests and chemotherapeutic approaches for SAC sarcocystosis.
Instituto de Patobiología
Fil: Wieser, Sarah Nathaly. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Wieser, Sarah Nathaly. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Decker Franco, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Decker Franco, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: De Alba, Paloma. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: De Alba, Paloma. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romero, Sandra Raquel. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación y Desarrollo Tecnológico para la Agricultura Familiar (CIPAF). Instituto de Investigación y Desarrollo Tecnológico para la Agricultura Familiar; Argentina
Fil: Ferrari, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ferrari, Alejandro. Instituto Nacional del Agua; Argentina
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Florin-Christensen, Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Parasitologia 3 (4) : 349–363 (Diciembre 2023)
Materia
Camelids
Sarcocystis
Sarcocystosis
Llamas
Alpacas
Diagnostic Techniques
Chemotherapeutants
Antigens
Camélidos
Sarcocistosis
Llama
Alpaca
Técnicas de Diagnosis
Productos Quimioterapeúticos
Antígenos
Sarcocystis aucheniae
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/16510
Acceder
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oai_identifier_str oai:localhost:20.500.12123/16510
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Discovery of antigens and cellular mechanisms in the protozoan parasite sarcocystis aucheniae using immunoproteomicsWieser, Sara NathalyDecker Franco, CeciliaDe Alba, PalomaRomero, Sandra RaquelFerrari, AlejandroSchnittger, LeonhardFlorin-Christensen, MonicaCamelidsSarcocystisSarcocystosisLlamasAlpacasDiagnostic TechniquesChemotherapeutantsAntigensCamélidosSarcocistosisLlamaAlpacaTécnicas de DiagnosisProductos QuimioterapeúticosAntígenosSarcocystis aucheniaeSarcocystis aucheniae is a coccidian parasite that produces macroscopic sarcocysts in South American camelid (SAC) muscles and causes a disease known as SAC sarcocystosis. This parasitosis hampers the commercialization of llama and alpaca meat, a vital economic activity in the Andean regions. No control or prevention methods are available, and diagnosis is based on postmortem visual inspection of carcasses. The aim of this study was to identify S. aucheniae B-cell epitopes suitable for the development of diagnostic methods for SAC sarcocystosis. To this end, sarcocyst immunoreactive protein bands were analyzed via mass spectrometry, and proteins in each band were identified in silico by searching in the parasite transcriptome. Five highly antigenic, hydrophilic B-cell epitopes, predicted not to cross-react with antibodies against other coccidia, were selected for future development of peptide-based serological tests. In addition, conserved domains present in the identified proteins allowed us to unravel metabolic pathways and mechanisms active in the parasitic stages present in sarcocysts, including aerobic respiration, antioxidant activity, signal transduction, protein synthesis and processing, and host–pathogen interactions. This study provides novel information on the biology of S. aucheniae, as well as new protein sequences that can be used for the development of diagnostic tests and chemotherapeutic approaches for SAC sarcocystosis.Instituto de PatobiologíaFil: Wieser, Sarah Nathaly. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: Wieser, Sarah Nathaly. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Decker Franco, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: Decker Franco, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: De Alba, Paloma. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: De Alba, Paloma. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Romero, Sandra Raquel. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación y Desarrollo Tecnológico para la Agricultura Familiar (CIPAF). Instituto de Investigación y Desarrollo Tecnológico para la Agricultura Familiar; ArgentinaFil: Ferrari, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ferrari, Alejandro. Instituto Nacional del Agua; ArgentinaFil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: Florin-Christensen, Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaMDPI2024-01-10T14:35:16Z2024-01-10T14:35:16Z2023-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/16510https://www.mdpi.com/2673-6772/3/4/342673-6772https://doi.org/10.3390/parasitologia3040034Parasitologia 3 (4) : 349–363 (Diciembre 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L01-I113, Herramientas de estudio de la Patogenia e Inmunidad en agentes infecciosos y tóxicos que aporten a la sustentabilidad de la producción pecuaria en el marco de Una Saludinfo:eu-repograntAgreement/INTA/2023-PD-L06-I114, Desarrollo y aplicación de métodos diagnósticos y epidemiológicos para la producción pecuaria sustentable y agroalimentaria en humanos con foco en Una Saludinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:50:10Zoai:localhost:20.500.12123/16510instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:50:11.752INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Discovery of antigens and cellular mechanisms in the protozoan parasite sarcocystis aucheniae using immunoproteomics
title Discovery of antigens and cellular mechanisms in the protozoan parasite sarcocystis aucheniae using immunoproteomics
spellingShingle Discovery of antigens and cellular mechanisms in the protozoan parasite sarcocystis aucheniae using immunoproteomics
Wieser, Sara Nathaly
Camelids
Sarcocystis
Sarcocystosis
Llamas
Alpacas
Diagnostic Techniques
Chemotherapeutants
Antigens
Camélidos
Sarcocistosis
Llama
Alpaca
Técnicas de Diagnosis
Productos Quimioterapeúticos
Antígenos
Sarcocystis aucheniae
title_short Discovery of antigens and cellular mechanisms in the protozoan parasite sarcocystis aucheniae using immunoproteomics
title_full Discovery of antigens and cellular mechanisms in the protozoan parasite sarcocystis aucheniae using immunoproteomics
title_fullStr Discovery of antigens and cellular mechanisms in the protozoan parasite sarcocystis aucheniae using immunoproteomics
title_full_unstemmed Discovery of antigens and cellular mechanisms in the protozoan parasite sarcocystis aucheniae using immunoproteomics
title_sort Discovery of antigens and cellular mechanisms in the protozoan parasite sarcocystis aucheniae using immunoproteomics
dc.creator.none.fl_str_mv Wieser, Sara Nathaly
Decker Franco, Cecilia
De Alba, Paloma
Romero, Sandra Raquel
Ferrari, Alejandro
Schnittger, Leonhard
Florin-Christensen, Monica
author Wieser, Sara Nathaly
author_facet Wieser, Sara Nathaly
Decker Franco, Cecilia
De Alba, Paloma
Romero, Sandra Raquel
Ferrari, Alejandro
Schnittger, Leonhard
Florin-Christensen, Monica
author_role author
author2 Decker Franco, Cecilia
De Alba, Paloma
Romero, Sandra Raquel
Ferrari, Alejandro
Schnittger, Leonhard
Florin-Christensen, Monica
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Camelids
Sarcocystis
Sarcocystosis
Llamas
Alpacas
Diagnostic Techniques
Chemotherapeutants
Antigens
Camélidos
Sarcocistosis
Llama
Alpaca
Técnicas de Diagnosis
Productos Quimioterapeúticos
Antígenos
Sarcocystis aucheniae
topic Camelids
Sarcocystis
Sarcocystosis
Llamas
Alpacas
Diagnostic Techniques
Chemotherapeutants
Antigens
Camélidos
Sarcocistosis
Llama
Alpaca
Técnicas de Diagnosis
Productos Quimioterapeúticos
Antígenos
Sarcocystis aucheniae
dc.description.none.fl_txt_mv Sarcocystis aucheniae is a coccidian parasite that produces macroscopic sarcocysts in South American camelid (SAC) muscles and causes a disease known as SAC sarcocystosis. This parasitosis hampers the commercialization of llama and alpaca meat, a vital economic activity in the Andean regions. No control or prevention methods are available, and diagnosis is based on postmortem visual inspection of carcasses. The aim of this study was to identify S. aucheniae B-cell epitopes suitable for the development of diagnostic methods for SAC sarcocystosis. To this end, sarcocyst immunoreactive protein bands were analyzed via mass spectrometry, and proteins in each band were identified in silico by searching in the parasite transcriptome. Five highly antigenic, hydrophilic B-cell epitopes, predicted not to cross-react with antibodies against other coccidia, were selected for future development of peptide-based serological tests. In addition, conserved domains present in the identified proteins allowed us to unravel metabolic pathways and mechanisms active in the parasitic stages present in sarcocysts, including aerobic respiration, antioxidant activity, signal transduction, protein synthesis and processing, and host–pathogen interactions. This study provides novel information on the biology of S. aucheniae, as well as new protein sequences that can be used for the development of diagnostic tests and chemotherapeutic approaches for SAC sarcocystosis.
Instituto de Patobiología
Fil: Wieser, Sarah Nathaly. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Wieser, Sarah Nathaly. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Decker Franco, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Decker Franco, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: De Alba, Paloma. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: De Alba, Paloma. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romero, Sandra Raquel. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación y Desarrollo Tecnológico para la Agricultura Familiar (CIPAF). Instituto de Investigación y Desarrollo Tecnológico para la Agricultura Familiar; Argentina
Fil: Ferrari, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ferrari, Alejandro. Instituto Nacional del Agua; Argentina
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Florin-Christensen, Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Sarcocystis aucheniae is a coccidian parasite that produces macroscopic sarcocysts in South American camelid (SAC) muscles and causes a disease known as SAC sarcocystosis. This parasitosis hampers the commercialization of llama and alpaca meat, a vital economic activity in the Andean regions. No control or prevention methods are available, and diagnosis is based on postmortem visual inspection of carcasses. The aim of this study was to identify S. aucheniae B-cell epitopes suitable for the development of diagnostic methods for SAC sarcocystosis. To this end, sarcocyst immunoreactive protein bands were analyzed via mass spectrometry, and proteins in each band were identified in silico by searching in the parasite transcriptome. Five highly antigenic, hydrophilic B-cell epitopes, predicted not to cross-react with antibodies against other coccidia, were selected for future development of peptide-based serological tests. In addition, conserved domains present in the identified proteins allowed us to unravel metabolic pathways and mechanisms active in the parasitic stages present in sarcocysts, including aerobic respiration, antioxidant activity, signal transduction, protein synthesis and processing, and host–pathogen interactions. This study provides novel information on the biology of S. aucheniae, as well as new protein sequences that can be used for the development of diagnostic tests and chemotherapeutic approaches for SAC sarcocystosis.
publishDate 2023
dc.date.none.fl_str_mv 2023-12
2024-01-10T14:35:16Z
2024-01-10T14:35:16Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/16510
https://www.mdpi.com/2673-6772/3/4/34
2673-6772
https://doi.org/10.3390/parasitologia3040034
url http://hdl.handle.net/20.500.12123/16510
https://www.mdpi.com/2673-6772/3/4/34
https://doi.org/10.3390/parasitologia3040034
identifier_str_mv 2673-6772
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L01-I113, Herramientas de estudio de la Patogenia e Inmunidad en agentes infecciosos y tóxicos que aporten a la sustentabilidad de la producción pecuaria en el marco de Una Salud
info:eu-repograntAgreement/INTA/2023-PD-L06-I114, Desarrollo y aplicación de métodos diagnósticos y epidemiológicos para la producción pecuaria sustentable y agroalimentaria en humanos con foco en Una Salud
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Parasitologia 3 (4) : 349–363 (Diciembre 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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