Molecular identification of Sarcocystis aucheniae as the macrocyst-forming parasite of llamas
- Autores
- Carletti, Tamara; Martin, Mara; Romero, Sandra Raquel; Morrison, David A.; Marcoppido, Gisela Ariana; Florin-Christensen, Monica; Schnittger, Leonhard
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The domestic South American camelids (SACs), llama (Lama glama) and alpaca (Lama paco), are frequently found to be infected with Sarcocystis parasites. Infections give rise in skeletal muscle to macroscopic cysts (1–5 mm long) that resemble rice seeds, each containing several million living bradyzoites. The finding of cysts prevents commercialization of SAC meat, an important source of income for rural families in the Andean flatlands. Thus, development of diagnostic methods to facilitate the control of these infections is highly desirable, and the first step to this end is the unequivocal species identification of the causative agent. Based on the cyst form and size, the infecting parasite has been described as Sarcocystis aucheniae; however, this traditional approach is not reliable as similar cysts may contain different species. To date, molecular identification has been done for a single isolate of S. aucheniae from an alpaca in Australia. In order to verify the identity of the species present in SACs of South America, the complete 18S rRNA gene was PCR-amplified and sequenced from macrocyst DNA obtained from three llamas of the Andean flatlands. A phylogenetic Bayesian analysis was carried out using the analyzed and available 18S rRNA sequences of Sarcocystis spp. In the constructed tree, all of the new 18S rRNA gene sequences segregated in a single clade together with the 18S rRNA gene sequence reported from an alpaca in Australia, demonstrating that the isolated parasite is S. aucheniae, and that this parasite indiscriminately infects both domestic SACs. This work represents the first molecular identification of the causative agent of SAC sarcocystiosis in South America, and can contribute to the development of control methods for this neglected parasitosis.
Instituto de Patobiología
Fil: Carletti, Tamara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Martin, Mara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Romero, Sandra Raquel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Abra Pampa; Argentina
Fil: Morrison, David A.. Swedish University of Agricultural Sciences. Department of Biochemical Sciences and Veterinary Public Health; Suecia
Fil: Marcoppido, Gisela Ariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Fuente
- Veterinary Parasitology 198 (3–4) : 396-400 (December 2013)
- Materia
-
Sarcocystis
Llama
Enfermedades de los Animales
Llamas
Animal Diseases
Parasites
Parásitos
Sarcocystis aucheniae - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
.jpg)
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/2019
Ver los metadatos del registro completo
| id |
INTADig_45bf80f68375fad9eb303e9026cf70c2 |
|---|---|
| oai_identifier_str |
oai:localhost:20.500.12123/2019 |
| network_acronym_str |
INTADig |
| repository_id_str |
l |
| network_name_str |
INTA Digital (INTA) |
| spelling |
Molecular identification of Sarcocystis aucheniae as the macrocyst-forming parasite of llamasCarletti, TamaraMartin, MaraRomero, Sandra RaquelMorrison, David A.Marcoppido, Gisela ArianaFlorin-Christensen, MonicaSchnittger, LeonhardSarcocystisLlamaEnfermedades de los AnimalesLlamasAnimal DiseasesParasitesParásitosSarcocystis aucheniaeThe domestic South American camelids (SACs), llama (Lama glama) and alpaca (Lama paco), are frequently found to be infected with Sarcocystis parasites. Infections give rise in skeletal muscle to macroscopic cysts (1–5 mm long) that resemble rice seeds, each containing several million living bradyzoites. The finding of cysts prevents commercialization of SAC meat, an important source of income for rural families in the Andean flatlands. Thus, development of diagnostic methods to facilitate the control of these infections is highly desirable, and the first step to this end is the unequivocal species identification of the causative agent. Based on the cyst form and size, the infecting parasite has been described as Sarcocystis aucheniae; however, this traditional approach is not reliable as similar cysts may contain different species. To date, molecular identification has been done for a single isolate of S. aucheniae from an alpaca in Australia. In order to verify the identity of the species present in SACs of South America, the complete 18S rRNA gene was PCR-amplified and sequenced from macrocyst DNA obtained from three llamas of the Andean flatlands. A phylogenetic Bayesian analysis was carried out using the analyzed and available 18S rRNA sequences of Sarcocystis spp. In the constructed tree, all of the new 18S rRNA gene sequences segregated in a single clade together with the 18S rRNA gene sequence reported from an alpaca in Australia, demonstrating that the isolated parasite is S. aucheniae, and that this parasite indiscriminately infects both domestic SACs. This work represents the first molecular identification of the causative agent of SAC sarcocystiosis in South America, and can contribute to the development of control methods for this neglected parasitosis.Instituto de PatobiologíaFil: Carletti, Tamara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Martin, Mara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Romero, Sandra Raquel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Abra Pampa; ArgentinaFil: Morrison, David A.. Swedish University of Agricultural Sciences. Department of Biochemical Sciences and Veterinary Public Health; SueciaFil: Marcoppido, Gisela Ariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina2018-03-14T12:17:07Z2018-03-14T12:17:07Z2013-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/2019https://www.sciencedirect.com/science/article/pii/S0304401713005013#!0304-4017https://doi.org/10.1016/j.vetpar.2013.09.007Veterinary Parasitology 198 (3–4) : 396-400 (December 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:16Zoai:localhost:20.500.12123/2019instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:16.485INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
| dc.title.none.fl_str_mv |
Molecular identification of Sarcocystis aucheniae as the macrocyst-forming parasite of llamas |
| title |
Molecular identification of Sarcocystis aucheniae as the macrocyst-forming parasite of llamas |
| spellingShingle |
Molecular identification of Sarcocystis aucheniae as the macrocyst-forming parasite of llamas Carletti, Tamara Sarcocystis Llama Enfermedades de los Animales Llamas Animal Diseases Parasites Parásitos Sarcocystis aucheniae |
| title_short |
Molecular identification of Sarcocystis aucheniae as the macrocyst-forming parasite of llamas |
| title_full |
Molecular identification of Sarcocystis aucheniae as the macrocyst-forming parasite of llamas |
| title_fullStr |
Molecular identification of Sarcocystis aucheniae as the macrocyst-forming parasite of llamas |
| title_full_unstemmed |
Molecular identification of Sarcocystis aucheniae as the macrocyst-forming parasite of llamas |
| title_sort |
Molecular identification of Sarcocystis aucheniae as the macrocyst-forming parasite of llamas |
| dc.creator.none.fl_str_mv |
Carletti, Tamara Martin, Mara Romero, Sandra Raquel Morrison, David A. Marcoppido, Gisela Ariana Florin-Christensen, Monica Schnittger, Leonhard |
| author |
Carletti, Tamara |
| author_facet |
Carletti, Tamara Martin, Mara Romero, Sandra Raquel Morrison, David A. Marcoppido, Gisela Ariana Florin-Christensen, Monica Schnittger, Leonhard |
| author_role |
author |
| author2 |
Martin, Mara Romero, Sandra Raquel Morrison, David A. Marcoppido, Gisela Ariana Florin-Christensen, Monica Schnittger, Leonhard |
| author2_role |
author author author author author author |
| dc.subject.none.fl_str_mv |
Sarcocystis Llama Enfermedades de los Animales Llamas Animal Diseases Parasites Parásitos Sarcocystis aucheniae |
| topic |
Sarcocystis Llama Enfermedades de los Animales Llamas Animal Diseases Parasites Parásitos Sarcocystis aucheniae |
| dc.description.none.fl_txt_mv |
The domestic South American camelids (SACs), llama (Lama glama) and alpaca (Lama paco), are frequently found to be infected with Sarcocystis parasites. Infections give rise in skeletal muscle to macroscopic cysts (1–5 mm long) that resemble rice seeds, each containing several million living bradyzoites. The finding of cysts prevents commercialization of SAC meat, an important source of income for rural families in the Andean flatlands. Thus, development of diagnostic methods to facilitate the control of these infections is highly desirable, and the first step to this end is the unequivocal species identification of the causative agent. Based on the cyst form and size, the infecting parasite has been described as Sarcocystis aucheniae; however, this traditional approach is not reliable as similar cysts may contain different species. To date, molecular identification has been done for a single isolate of S. aucheniae from an alpaca in Australia. In order to verify the identity of the species present in SACs of South America, the complete 18S rRNA gene was PCR-amplified and sequenced from macrocyst DNA obtained from three llamas of the Andean flatlands. A phylogenetic Bayesian analysis was carried out using the analyzed and available 18S rRNA sequences of Sarcocystis spp. In the constructed tree, all of the new 18S rRNA gene sequences segregated in a single clade together with the 18S rRNA gene sequence reported from an alpaca in Australia, demonstrating that the isolated parasite is S. aucheniae, and that this parasite indiscriminately infects both domestic SACs. This work represents the first molecular identification of the causative agent of SAC sarcocystiosis in South America, and can contribute to the development of control methods for this neglected parasitosis. Instituto de Patobiología Fil: Carletti, Tamara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Martin, Mara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Romero, Sandra Raquel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Abra Pampa; Argentina Fil: Morrison, David A.. Swedish University of Agricultural Sciences. Department of Biochemical Sciences and Veterinary Public Health; Suecia Fil: Marcoppido, Gisela Ariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| description |
The domestic South American camelids (SACs), llama (Lama glama) and alpaca (Lama paco), are frequently found to be infected with Sarcocystis parasites. Infections give rise in skeletal muscle to macroscopic cysts (1–5 mm long) that resemble rice seeds, each containing several million living bradyzoites. The finding of cysts prevents commercialization of SAC meat, an important source of income for rural families in the Andean flatlands. Thus, development of diagnostic methods to facilitate the control of these infections is highly desirable, and the first step to this end is the unequivocal species identification of the causative agent. Based on the cyst form and size, the infecting parasite has been described as Sarcocystis aucheniae; however, this traditional approach is not reliable as similar cysts may contain different species. To date, molecular identification has been done for a single isolate of S. aucheniae from an alpaca in Australia. In order to verify the identity of the species present in SACs of South America, the complete 18S rRNA gene was PCR-amplified and sequenced from macrocyst DNA obtained from three llamas of the Andean flatlands. A phylogenetic Bayesian analysis was carried out using the analyzed and available 18S rRNA sequences of Sarcocystis spp. In the constructed tree, all of the new 18S rRNA gene sequences segregated in a single clade together with the 18S rRNA gene sequence reported from an alpaca in Australia, demonstrating that the isolated parasite is S. aucheniae, and that this parasite indiscriminately infects both domestic SACs. This work represents the first molecular identification of the causative agent of SAC sarcocystiosis in South America, and can contribute to the development of control methods for this neglected parasitosis. |
| publishDate |
2013 |
| dc.date.none.fl_str_mv |
2013-12 2018-03-14T12:17:07Z 2018-03-14T12:17:07Z |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/2019 https://www.sciencedirect.com/science/article/pii/S0304401713005013#! 0304-4017 https://doi.org/10.1016/j.vetpar.2013.09.007 |
| url |
http://hdl.handle.net/20.500.12123/2019 https://www.sciencedirect.com/science/article/pii/S0304401713005013#! https://doi.org/10.1016/j.vetpar.2013.09.007 |
| identifier_str_mv |
0304-4017 |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
| eu_rights_str_mv |
restrictedAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.source.none.fl_str_mv |
Veterinary Parasitology 198 (3–4) : 396-400 (December 2013) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
| reponame_str |
INTA Digital (INTA) |
| collection |
INTA Digital (INTA) |
| instname_str |
Instituto Nacional de Tecnología Agropecuaria |
| repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
| repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
| _version_ |
1844619120589406208 |
| score |
12.559606 |