Discovery of Antigens and Cellular Mechanisms in the Protozoan Parasite Sarcocystis aucheniae Using Immunoproteomics

Autores
Wieser, Sarah Nathaly; Decker Franco, Cecilia; de Alba, Paloma; Romero, Sandra; Ferrari, Alejandro; Schnittger, Leonhard; Jacobsen, Monica Ofelia
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sarcocystis aucheniae is a coccidian parasite that produces macroscopic sarcocysts in South American camelid (SAC) muscles and causes a disease known as SAC sarcocystosis. This parasitosis hampers the commercialization of llama and alpaca meat, a vital economic activity in the Andean regions. No control or prevention methods are available, and diagnosis is based on postmortem visual inspection of carcasses. The aim of this study was to identify S. aucheniae B-cell epitopes suitable for the development of diagnostic methods for SAC sarcocystosis. To this end, sarcocyst immunoreactive protein bands were analyzed via mass spectrometry, and proteins in each band were identified in silico by searching in the parasite transcriptome. Five highly antigenic, hydrophilic B-cell epitopes, predicted not to cross-react with antibodies against other coccidia, were selected for future development of peptide-based serological tests. In addition, conserved domains present in the identified proteins allowed us to unravel metabolic pathways and mechanisms active in the parasitic stages present in sarcocysts, including aerobic respiration, antioxidant activity, signal transduction, protein synthesis and processing, and host–pathogen interactions. This study provides novel information on the biology of S. aucheniae, as well as new protein sequences that can be used for the development of diagnostic tests and chemotherapeutic approaches for SAC sarcocystosis.
Fil: Wieser, Sarah Nathaly. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; Argentina
Fil: Decker Franco, Cecilia. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; Argentina
Fil: de Alba, Paloma. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; Argentina
Fil: Romero, Sandra. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion y Desarrollo Tecnologico Para la Agricultura Familiar. Instituto de Investigacion y Desarrollo Tecnologico Para la Agricultura Familiar Region Noa.; Argentina
Fil: Ferrari, Alejandro. Instituto Nacional del Agua; Argentina
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; Argentina
Fil: Jacobsen, Monica Ofelia. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; Argentina
Materia
ALPACAS
B-CELL EPITOPES
CHEMOTHERAPEUTIC TARGETS
DIAGNOSTICS
LLAMAS
MACROSCOPIC SARCOCYSTS
SARCOCYSTOSIS
SOUTH AMERICAN CAMELIDS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/229100

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oai_identifier_str oai:ri.conicet.gov.ar:11336/229100
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Discovery of Antigens and Cellular Mechanisms in the Protozoan Parasite Sarcocystis aucheniae Using ImmunoproteomicsWieser, Sarah NathalyDecker Franco, Ceciliade Alba, PalomaRomero, SandraFerrari, AlejandroSchnittger, LeonhardJacobsen, Monica OfeliaALPACASB-CELL EPITOPESCHEMOTHERAPEUTIC TARGETSDIAGNOSTICSLLAMASMACROSCOPIC SARCOCYSTSSARCOCYSTOSISSOUTH AMERICAN CAMELIDShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Sarcocystis aucheniae is a coccidian parasite that produces macroscopic sarcocysts in South American camelid (SAC) muscles and causes a disease known as SAC sarcocystosis. This parasitosis hampers the commercialization of llama and alpaca meat, a vital economic activity in the Andean regions. No control or prevention methods are available, and diagnosis is based on postmortem visual inspection of carcasses. The aim of this study was to identify S. aucheniae B-cell epitopes suitable for the development of diagnostic methods for SAC sarcocystosis. To this end, sarcocyst immunoreactive protein bands were analyzed via mass spectrometry, and proteins in each band were identified in silico by searching in the parasite transcriptome. Five highly antigenic, hydrophilic B-cell epitopes, predicted not to cross-react with antibodies against other coccidia, were selected for future development of peptide-based serological tests. In addition, conserved domains present in the identified proteins allowed us to unravel metabolic pathways and mechanisms active in the parasitic stages present in sarcocysts, including aerobic respiration, antioxidant activity, signal transduction, protein synthesis and processing, and host–pathogen interactions. This study provides novel information on the biology of S. aucheniae, as well as new protein sequences that can be used for the development of diagnostic tests and chemotherapeutic approaches for SAC sarcocystosis.Fil: Wieser, Sarah Nathaly. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; ArgentinaFil: Decker Franco, Cecilia. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; ArgentinaFil: de Alba, Paloma. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; ArgentinaFil: Romero, Sandra. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion y Desarrollo Tecnologico Para la Agricultura Familiar. Instituto de Investigacion y Desarrollo Tecnologico Para la Agricultura Familiar Region Noa.; ArgentinaFil: Ferrari, Alejandro. Instituto Nacional del Agua; ArgentinaFil: Schnittger, Leonhard. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; ArgentinaFil: Jacobsen, Monica Ofelia. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; ArgentinaMDPI2023-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/229100Wieser, Sarah Nathaly; Decker Franco, Cecilia; de Alba, Paloma; Romero, Sandra; Ferrari, Alejandro; et al.; Discovery of Antigens and Cellular Mechanisms in the Protozoan Parasite Sarcocystis aucheniae Using Immunoproteomics; MDPI; Parasitologia; 3; 4; 11-2023; 349-3632673-6772CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2673-6772/3/4/34info:eu-repo/semantics/altIdentifier/doi/10.3390/parasitologia3040034info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:22Zoai:ri.conicet.gov.ar:11336/229100instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:23.213CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Discovery of Antigens and Cellular Mechanisms in the Protozoan Parasite Sarcocystis aucheniae Using Immunoproteomics
title Discovery of Antigens and Cellular Mechanisms in the Protozoan Parasite Sarcocystis aucheniae Using Immunoproteomics
spellingShingle Discovery of Antigens and Cellular Mechanisms in the Protozoan Parasite Sarcocystis aucheniae Using Immunoproteomics
Wieser, Sarah Nathaly
ALPACAS
B-CELL EPITOPES
CHEMOTHERAPEUTIC TARGETS
DIAGNOSTICS
LLAMAS
MACROSCOPIC SARCOCYSTS
SARCOCYSTOSIS
SOUTH AMERICAN CAMELIDS
title_short Discovery of Antigens and Cellular Mechanisms in the Protozoan Parasite Sarcocystis aucheniae Using Immunoproteomics
title_full Discovery of Antigens and Cellular Mechanisms in the Protozoan Parasite Sarcocystis aucheniae Using Immunoproteomics
title_fullStr Discovery of Antigens and Cellular Mechanisms in the Protozoan Parasite Sarcocystis aucheniae Using Immunoproteomics
title_full_unstemmed Discovery of Antigens and Cellular Mechanisms in the Protozoan Parasite Sarcocystis aucheniae Using Immunoproteomics
title_sort Discovery of Antigens and Cellular Mechanisms in the Protozoan Parasite Sarcocystis aucheniae Using Immunoproteomics
dc.creator.none.fl_str_mv Wieser, Sarah Nathaly
Decker Franco, Cecilia
de Alba, Paloma
Romero, Sandra
Ferrari, Alejandro
Schnittger, Leonhard
Jacobsen, Monica Ofelia
author Wieser, Sarah Nathaly
author_facet Wieser, Sarah Nathaly
Decker Franco, Cecilia
de Alba, Paloma
Romero, Sandra
Ferrari, Alejandro
Schnittger, Leonhard
Jacobsen, Monica Ofelia
author_role author
author2 Decker Franco, Cecilia
de Alba, Paloma
Romero, Sandra
Ferrari, Alejandro
Schnittger, Leonhard
Jacobsen, Monica Ofelia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ALPACAS
B-CELL EPITOPES
CHEMOTHERAPEUTIC TARGETS
DIAGNOSTICS
LLAMAS
MACROSCOPIC SARCOCYSTS
SARCOCYSTOSIS
SOUTH AMERICAN CAMELIDS
topic ALPACAS
B-CELL EPITOPES
CHEMOTHERAPEUTIC TARGETS
DIAGNOSTICS
LLAMAS
MACROSCOPIC SARCOCYSTS
SARCOCYSTOSIS
SOUTH AMERICAN CAMELIDS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Sarcocystis aucheniae is a coccidian parasite that produces macroscopic sarcocysts in South American camelid (SAC) muscles and causes a disease known as SAC sarcocystosis. This parasitosis hampers the commercialization of llama and alpaca meat, a vital economic activity in the Andean regions. No control or prevention methods are available, and diagnosis is based on postmortem visual inspection of carcasses. The aim of this study was to identify S. aucheniae B-cell epitopes suitable for the development of diagnostic methods for SAC sarcocystosis. To this end, sarcocyst immunoreactive protein bands were analyzed via mass spectrometry, and proteins in each band were identified in silico by searching in the parasite transcriptome. Five highly antigenic, hydrophilic B-cell epitopes, predicted not to cross-react with antibodies against other coccidia, were selected for future development of peptide-based serological tests. In addition, conserved domains present in the identified proteins allowed us to unravel metabolic pathways and mechanisms active in the parasitic stages present in sarcocysts, including aerobic respiration, antioxidant activity, signal transduction, protein synthesis and processing, and host–pathogen interactions. This study provides novel information on the biology of S. aucheniae, as well as new protein sequences that can be used for the development of diagnostic tests and chemotherapeutic approaches for SAC sarcocystosis.
Fil: Wieser, Sarah Nathaly. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; Argentina
Fil: Decker Franco, Cecilia. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; Argentina
Fil: de Alba, Paloma. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; Argentina
Fil: Romero, Sandra. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion y Desarrollo Tecnologico Para la Agricultura Familiar. Instituto de Investigacion y Desarrollo Tecnologico Para la Agricultura Familiar Region Noa.; Argentina
Fil: Ferrari, Alejandro. Instituto Nacional del Agua; Argentina
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; Argentina
Fil: Jacobsen, Monica Ofelia. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Patobiologia Veterinaria. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Patobiologia Veterinaria.; Argentina
description Sarcocystis aucheniae is a coccidian parasite that produces macroscopic sarcocysts in South American camelid (SAC) muscles and causes a disease known as SAC sarcocystosis. This parasitosis hampers the commercialization of llama and alpaca meat, a vital economic activity in the Andean regions. No control or prevention methods are available, and diagnosis is based on postmortem visual inspection of carcasses. The aim of this study was to identify S. aucheniae B-cell epitopes suitable for the development of diagnostic methods for SAC sarcocystosis. To this end, sarcocyst immunoreactive protein bands were analyzed via mass spectrometry, and proteins in each band were identified in silico by searching in the parasite transcriptome. Five highly antigenic, hydrophilic B-cell epitopes, predicted not to cross-react with antibodies against other coccidia, were selected for future development of peptide-based serological tests. In addition, conserved domains present in the identified proteins allowed us to unravel metabolic pathways and mechanisms active in the parasitic stages present in sarcocysts, including aerobic respiration, antioxidant activity, signal transduction, protein synthesis and processing, and host–pathogen interactions. This study provides novel information on the biology of S. aucheniae, as well as new protein sequences that can be used for the development of diagnostic tests and chemotherapeutic approaches for SAC sarcocystosis.
publishDate 2023
dc.date.none.fl_str_mv 2023-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/229100
Wieser, Sarah Nathaly; Decker Franco, Cecilia; de Alba, Paloma; Romero, Sandra; Ferrari, Alejandro; et al.; Discovery of Antigens and Cellular Mechanisms in the Protozoan Parasite Sarcocystis aucheniae Using Immunoproteomics; MDPI; Parasitologia; 3; 4; 11-2023; 349-363
2673-6772
CONICET Digital
CONICET
url http://hdl.handle.net/11336/229100
identifier_str_mv Wieser, Sarah Nathaly; Decker Franco, Cecilia; de Alba, Paloma; Romero, Sandra; Ferrari, Alejandro; et al.; Discovery of Antigens and Cellular Mechanisms in the Protozoan Parasite Sarcocystis aucheniae Using Immunoproteomics; MDPI; Parasitologia; 3; 4; 11-2023; 349-363
2673-6772
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2673-6772/3/4/34
info:eu-repo/semantics/altIdentifier/doi/10.3390/parasitologia3040034
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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