Expression and mapping of anthocyanin biosynthesis genes in carrot

• Autores: Yildiz, Mehtap; Willis, David K.; Cavagnaro, Pablo; Iorizzo, Massimo; Abak, Kazim; Simon, Philipp W.
• Año de publicación: 2013
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Theoretical and Applied Genetics 126 (7) : 1689–1702 (July 2013)
• Materia: Zanahoria; Antocianinas; Mapas Genéticos; Expresión Génica; Carrots; Anthocyanins; Genetic Maps; Gene Expression
• Institución: Instituto Nacional de Tecnología Agropecuaria

Anthocyanin gene expression has been extensively studied in leaves, fruits and flowers of numerous plants. Little, however, is known about anthocyanin accumulation in roots of carrots or other species. We quantified expression of six anthocyanin biosynthetic genes [phenylalanine ammonia-lyase (PAL3), chalcone synthase (CHS1), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR1), leucoanthocyanidin dioxygenase (LDOX2), and UDP-glucose:flavonoid 3-O-glucosyltransferase (UFGT)] in three carrot inbreds with contrasting root color: solid purple (phloem and xylem); purple outer phloem/orange xylem; and orange phloem and xylem. Transcripts for five of these genes (CHS1, DFR1, F3H, LDOX2, PAL3) accumulated at high levels in solid purple carrots, less in purple-orange carrot, and low or no transcript in orange carrots. Gene expression coincided with anthocyanin accumulation. In contrast, UFGT expression was comparable in purple and orange carrots and relatively unchanged during root development. In addition, five anthocyanin biosynthesis genes [FLS1 (flavonol synthase), F3H, LDOX2, PAL3, and UFGT] and three anthocyanin transcription factors (DcEFR1, DcMYB3 and DcMYB5) were mapped in a population segregating for the P 1 locus that conditions purple root color. P 1 mapped to chromosome 3 and of the eight anthocyanin biosynthesis genes, only F3H and FLS1 were linked to P 1. The gene expression and mapping data suggest a coordinated regulatory control of anthocyanin expression in carrot root and establish a framework for studying the anthocyanin pathway in carrots, and they also suggest that none of the genes evaluated is a candidate for P 1.
EEA La Consulta
Fil: Yildiz, Mehtap. University of Wisconsin. Department of Horticulture; Estados Unidos. Yuzuncu Yil University. Faculty of Agriculture. Department of Agricultural Biotechnology; Turquía
Fil: Willis, David K.. University of Wisconsin-Madison. Department of Plant Pathology ; Estados Unidos. United States Department of Agriculture–Agricultural Research Service. Vegetable Crops Research Unit; Estados Unidos
Fil: Cavagnaro, Pablo Federico. University of Wisconsin. Department of Horticulture; Estados Unidos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Iorizzo, Massimo. University of Wisconsin. Department of Horticulture; Estados Unidos
Fil: Abak, Kazim. Lefke European University. Faculty of Agricultural Science and Technology. Department of Horticulture Production and Marketing; Turquía
Fil: Simon, Philipp W. University of Wisconsin. Department of Horticulture; Estados Unidos. United States Department of Agriculture–Agricultural Research Service. Vegetable Crops Research Unit; Estados Unidos