Relationships among Gene Expression and Anthocyanin Composition of Malbec Grapevine Clones

Autores
Muñoz, Claudio Javier; Gomez Talquenca, Gonzalo; Chialva, Constanza Soledad; Ibáñez, Javier; Martínez-Zapater, José M.; Peña Neira, Alvaro; Lijavetzky, Diego
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Anthocyanin profiles are commonly used for grapevine cultivar identification because it is currently accepted that this trait is closely related to their genetic characteristics. Nevertheless, the extent of the variation for the anthocyanin profiles among clones of the same cultivar has not yet been studied in depth. The relative concentration of anthocyanins of 131 Malbec clones grown in the same vineyard was investigated by HPLC-DAD and the use of comprehensive statistic procedures. Complementarily, the expression level of structural and regulatory genes was studied via real time polymerase chain reaction. Significant variation was identified among the profiles of the clones, mainly due to variations in the amounts of malvidin derivatives. Finally, the differential expression in F3′5′H, OMT1 and AM2 genes seems to be related to the malvidin content variation. This work shows the existence of variation for the anthocyanin profiles among clones from the same grapevine cultivar and the putative involvement of genes related to hydroxylation, methylation, and transport of anthocyanins on the basis of such variation.
EEA Mendoza
Fil: Muñoz, Claudio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Gomez Talquenca, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitovirología; Argentina
Fil: Chialva, Constanza Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Ibáñez, Javier. Consejo Superior de Investigaciones Científicas (CSIC). Centro Nacional de Biotecnología. Departamento de Genética Molecular de Plantas; España. Consejo Superior de Investigaciones Científicas, Universidad de La Rioja, Gobierno de La Rioja. Instituto de Ciencias de la Vid y del Vino; España
Fil: Peña Neira, Álvaro. Universidad de Chile. Facultad de Ciencias Agronómicas. Departamento de Agroindustria y Enologı́a; Chile
Fil: Lijavetzky, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fuente
Journal of agricuitural and food chemistry 62 (28) : 6716–6725. (2014)
Materia
Vid
Variedades
Clones
Expresión Génica
Antocianinas
Grapevines
Varieties
Gene Expression
Anthocyanins
Malbec
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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Acceder
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oai_identifier_str oai:localhost:20.500.12123/2480
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spelling Relationships among Gene Expression and Anthocyanin Composition of Malbec Grapevine ClonesMuñoz, Claudio JavierGomez Talquenca, GonzaloChialva, Constanza SoledadIbáñez, JavierMartínez-Zapater, José M.Peña Neira, AlvaroLijavetzky, DiegoVidVariedadesClonesExpresión GénicaAntocianinasGrapevinesVarietiesGene ExpressionAnthocyaninsMalbecAnthocyanin profiles are commonly used for grapevine cultivar identification because it is currently accepted that this trait is closely related to their genetic characteristics. Nevertheless, the extent of the variation for the anthocyanin profiles among clones of the same cultivar has not yet been studied in depth. The relative concentration of anthocyanins of 131 Malbec clones grown in the same vineyard was investigated by HPLC-DAD and the use of comprehensive statistic procedures. Complementarily, the expression level of structural and regulatory genes was studied via real time polymerase chain reaction. Significant variation was identified among the profiles of the clones, mainly due to variations in the amounts of malvidin derivatives. Finally, the differential expression in F3′5′H, OMT1 and AM2 genes seems to be related to the malvidin content variation. This work shows the existence of variation for the anthocyanin profiles among clones from the same grapevine cultivar and the putative involvement of genes related to hydroxylation, methylation, and transport of anthocyanins on the basis of such variation.EEA MendozaFil: Muñoz, Claudio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Gomez Talquenca, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitovirología; ArgentinaFil: Chialva, Constanza Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Ibáñez, Javier. Consejo Superior de Investigaciones Científicas (CSIC). Centro Nacional de Biotecnología. Departamento de Genética Molecular de Plantas; España. Consejo Superior de Investigaciones Científicas, Universidad de La Rioja, Gobierno de La Rioja. Instituto de Ciencias de la Vid y del Vino; EspañaFil: Peña Neira, Álvaro. Universidad de Chile. Facultad de Ciencias Agronómicas. Departamento de Agroindustria y Enologı́a; ChileFil: Lijavetzky, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina2018-05-24T14:06:12Z2018-05-24T14:06:12Z2014-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://pubs.acs.org/doi/abs/10.1021/jf501575mhttp://hdl.handle.net/20.500.12123/24800021-85611520-5118http://dx.doi.org/10.1021/jf501575mJournal of agricuitural and food chemistry 62 (28) : 6716–6725. (2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:18Zoai:localhost:20.500.12123/2480instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:19.188INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Relationships among Gene Expression and Anthocyanin Composition of Malbec Grapevine Clones
title Relationships among Gene Expression and Anthocyanin Composition of Malbec Grapevine Clones
spellingShingle Relationships among Gene Expression and Anthocyanin Composition of Malbec Grapevine Clones
Muñoz, Claudio Javier
Vid
Variedades
Clones
Expresión Génica
Antocianinas
Grapevines
Varieties
Gene Expression
Anthocyanins
Malbec
title_short Relationships among Gene Expression and Anthocyanin Composition of Malbec Grapevine Clones
title_full Relationships among Gene Expression and Anthocyanin Composition of Malbec Grapevine Clones
title_fullStr Relationships among Gene Expression and Anthocyanin Composition of Malbec Grapevine Clones
title_full_unstemmed Relationships among Gene Expression and Anthocyanin Composition of Malbec Grapevine Clones
title_sort Relationships among Gene Expression and Anthocyanin Composition of Malbec Grapevine Clones
dc.creator.none.fl_str_mv Muñoz, Claudio Javier
Gomez Talquenca, Gonzalo
Chialva, Constanza Soledad
Ibáñez, Javier
Martínez-Zapater, José M.
Peña Neira, Alvaro
Lijavetzky, Diego
author Muñoz, Claudio Javier
author_facet Muñoz, Claudio Javier
Gomez Talquenca, Gonzalo
Chialva, Constanza Soledad
Ibáñez, Javier
Martínez-Zapater, José M.
Peña Neira, Alvaro
Lijavetzky, Diego
author_role author
author2 Gomez Talquenca, Gonzalo
Chialva, Constanza Soledad
Ibáñez, Javier
Martínez-Zapater, José M.
Peña Neira, Alvaro
Lijavetzky, Diego
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Vid
Variedades
Clones
Expresión Génica
Antocianinas
Grapevines
Varieties
Gene Expression
Anthocyanins
Malbec
topic Vid
Variedades
Clones
Expresión Génica
Antocianinas
Grapevines
Varieties
Gene Expression
Anthocyanins
Malbec
dc.description.none.fl_txt_mv Anthocyanin profiles are commonly used for grapevine cultivar identification because it is currently accepted that this trait is closely related to their genetic characteristics. Nevertheless, the extent of the variation for the anthocyanin profiles among clones of the same cultivar has not yet been studied in depth. The relative concentration of anthocyanins of 131 Malbec clones grown in the same vineyard was investigated by HPLC-DAD and the use of comprehensive statistic procedures. Complementarily, the expression level of structural and regulatory genes was studied via real time polymerase chain reaction. Significant variation was identified among the profiles of the clones, mainly due to variations in the amounts of malvidin derivatives. Finally, the differential expression in F3′5′H, OMT1 and AM2 genes seems to be related to the malvidin content variation. This work shows the existence of variation for the anthocyanin profiles among clones from the same grapevine cultivar and the putative involvement of genes related to hydroxylation, methylation, and transport of anthocyanins on the basis of such variation.
EEA Mendoza
Fil: Muñoz, Claudio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Gomez Talquenca, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Laboratorio de Fitovirología; Argentina
Fil: Chialva, Constanza Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Ibáñez, Javier. Consejo Superior de Investigaciones Científicas (CSIC). Centro Nacional de Biotecnología. Departamento de Genética Molecular de Plantas; España. Consejo Superior de Investigaciones Científicas, Universidad de La Rioja, Gobierno de La Rioja. Instituto de Ciencias de la Vid y del Vino; España
Fil: Peña Neira, Álvaro. Universidad de Chile. Facultad de Ciencias Agronómicas. Departamento de Agroindustria y Enologı́a; Chile
Fil: Lijavetzky, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
description Anthocyanin profiles are commonly used for grapevine cultivar identification because it is currently accepted that this trait is closely related to their genetic characteristics. Nevertheless, the extent of the variation for the anthocyanin profiles among clones of the same cultivar has not yet been studied in depth. The relative concentration of anthocyanins of 131 Malbec clones grown in the same vineyard was investigated by HPLC-DAD and the use of comprehensive statistic procedures. Complementarily, the expression level of structural and regulatory genes was studied via real time polymerase chain reaction. Significant variation was identified among the profiles of the clones, mainly due to variations in the amounts of malvidin derivatives. Finally, the differential expression in F3′5′H, OMT1 and AM2 genes seems to be related to the malvidin content variation. This work shows the existence of variation for the anthocyanin profiles among clones from the same grapevine cultivar and the putative involvement of genes related to hydroxylation, methylation, and transport of anthocyanins on the basis of such variation.
publishDate 2014
dc.date.none.fl_str_mv 2014-07
2018-05-24T14:06:12Z
2018-05-24T14:06:12Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://pubs.acs.org/doi/abs/10.1021/jf501575m
http://hdl.handle.net/20.500.12123/2480
0021-8561
1520-5118
http://dx.doi.org/10.1021/jf501575m
url https://pubs.acs.org/doi/abs/10.1021/jf501575m
http://hdl.handle.net/20.500.12123/2480
http://dx.doi.org/10.1021/jf501575m
identifier_str_mv 0021-8561
1520-5118
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Journal of agricuitural and food chemistry 62 (28) : 6716–6725. (2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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