Functional characterization of DcMYB11, an R2R3 MYB associated with the purple pigmentation of carrot petiole

Autores
D’Amelia, Vincenzo; Curaba, Julien; Abid, Muhammad Ali; Esposito, Salvatore; Cavagnaro, Pablo; Carputo, Domenico; Iorizzo, Massimo
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The accumulation of anthocyanin pigments can exhibit different patterns across plant tissues and crop varieties. This variability allowed the investigation of the molecular mechanisms behind the biosynthesis of these pigments in several plant species. Among crops, carrots have a well-defined anthocyanin pigmentation pattern depending on the genic background. In this work, we report on the discovery of DNA structural differences affecting the activity of an R2R3 MYB (encoded by DcMYB11) involved in anthocyanin regulation in carrot petiole. To this end, we first verified the function of DcMYB11 using heterologous systems and identified three different alleles which may explain differences in petiole pigmentation. Characterization of the DcMYB11 alleles at the 5' upstream sequence unveiled a sequence that functions as a putative enhancer. In conclusion, this study provides novel insight into the molecular mechanisms controlling anthocyanin accumulation in carrot. By these outcomes, we expanded our knowledge on the cis-regulatory sequences in plants.
EEA La Consulta
Fil: D’Amelia, Vincenzo. National Research Council. Institute of Bioscience and BioResources; Italia
Fil: D’Amelia, Vincenzo. University of Naples Federico II. Department of Agricultural Sciences; Italia
Fil: Curaba, Julien. North Carolina State University. Horticulture Science Department. Plants for Human Health Institute; Estados Unidos
Fil: Abid, Muhammad Ali. North Carolina State University. Horticulture Science Department. Plants for Human Health Institute; Estados Unidos
Fil: Esposito, Salvatore. CREA Research Centre for Cereal and Industrial Crops; Italia
Fil: Cavagnaro, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Cavagnaro, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina.
Fil: Carputo, Domenico. University of Naples Federico II. Department of Agricultural Sciences; Italia
Fil: Iorizzo, Massimo. North Carolina State University. Horticulture Science Department. Plants for Human Health Institute; Estados Unidos
Fuente
Planta 258 : Article number: 50 (July 2023)
Materia
Zanahoria
Pigmentación
Variedades
Daucus carota
Antocianinas
Carrots
Pigmentation
Varieties
Anthocyanins
Zanahoria Morada
Purple Carrots
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/14846
Acceder
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oai_identifier_str oai:localhost:20.500.12123/14846
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network_name_str INTA Digital (INTA)
spelling Functional characterization of DcMYB11, an R2R3 MYB associated with the purple pigmentation of carrot petioleD’Amelia, VincenzoCuraba, JulienAbid, Muhammad AliEsposito, SalvatoreCavagnaro, PabloCarputo, DomenicoIorizzo, MassimoZanahoriaPigmentaciónVariedadesDaucus carotaAntocianinasCarrotsPigmentationVarietiesAnthocyaninsZanahoria MoradaPurple CarrotsThe accumulation of anthocyanin pigments can exhibit different patterns across plant tissues and crop varieties. This variability allowed the investigation of the molecular mechanisms behind the biosynthesis of these pigments in several plant species. Among crops, carrots have a well-defined anthocyanin pigmentation pattern depending on the genic background. In this work, we report on the discovery of DNA structural differences affecting the activity of an R2R3 MYB (encoded by DcMYB11) involved in anthocyanin regulation in carrot petiole. To this end, we first verified the function of DcMYB11 using heterologous systems and identified three different alleles which may explain differences in petiole pigmentation. Characterization of the DcMYB11 alleles at the 5' upstream sequence unveiled a sequence that functions as a putative enhancer. In conclusion, this study provides novel insight into the molecular mechanisms controlling anthocyanin accumulation in carrot. By these outcomes, we expanded our knowledge on the cis-regulatory sequences in plants.EEA La ConsultaFil: D’Amelia, Vincenzo. National Research Council. Institute of Bioscience and BioResources; ItaliaFil: D’Amelia, Vincenzo. University of Naples Federico II. Department of Agricultural Sciences; ItaliaFil: Curaba, Julien. North Carolina State University. Horticulture Science Department. Plants for Human Health Institute; Estados UnidosFil: Abid, Muhammad Ali. North Carolina State University. Horticulture Science Department. Plants for Human Health Institute; Estados UnidosFil: Esposito, Salvatore. CREA Research Centre for Cereal and Industrial Crops; ItaliaFil: Cavagnaro, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Cavagnaro, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina.Fil: Carputo, Domenico. University of Naples Federico II. Department of Agricultural Sciences; ItaliaFil: Iorizzo, Massimo. North Carolina State University. Horticulture Science Department. Plants for Human Health Institute; Estados UnidosSpringer2023-08-01T11:26:52Z2023-08-01T11:26:52Z2023-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/14846https://link.springer.com/article/10.1007/s00425-023-04200-50032-09351432-2048https://doi.org/10.1007/s00425-023-04200-5Planta 258 : Article number: 50 (July 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-11T10:24:39Zoai:localhost:20.500.12123/14846instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-11 10:24:39.54INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Functional characterization of DcMYB11, an R2R3 MYB associated with the purple pigmentation of carrot petiole
title Functional characterization of DcMYB11, an R2R3 MYB associated with the purple pigmentation of carrot petiole
spellingShingle Functional characterization of DcMYB11, an R2R3 MYB associated with the purple pigmentation of carrot petiole
D’Amelia, Vincenzo
Zanahoria
Pigmentación
Variedades
Daucus carota
Antocianinas
Carrots
Pigmentation
Varieties
Anthocyanins
Zanahoria Morada
Purple Carrots
title_short Functional characterization of DcMYB11, an R2R3 MYB associated with the purple pigmentation of carrot petiole
title_full Functional characterization of DcMYB11, an R2R3 MYB associated with the purple pigmentation of carrot petiole
title_fullStr Functional characterization of DcMYB11, an R2R3 MYB associated with the purple pigmentation of carrot petiole
title_full_unstemmed Functional characterization of DcMYB11, an R2R3 MYB associated with the purple pigmentation of carrot petiole
title_sort Functional characterization of DcMYB11, an R2R3 MYB associated with the purple pigmentation of carrot petiole
dc.creator.none.fl_str_mv D’Amelia, Vincenzo
Curaba, Julien
Abid, Muhammad Ali
Esposito, Salvatore
Cavagnaro, Pablo
Carputo, Domenico
Iorizzo, Massimo
author D’Amelia, Vincenzo
author_facet D’Amelia, Vincenzo
Curaba, Julien
Abid, Muhammad Ali
Esposito, Salvatore
Cavagnaro, Pablo
Carputo, Domenico
Iorizzo, Massimo
author_role author
author2 Curaba, Julien
Abid, Muhammad Ali
Esposito, Salvatore
Cavagnaro, Pablo
Carputo, Domenico
Iorizzo, Massimo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Zanahoria
Pigmentación
Variedades
Daucus carota
Antocianinas
Carrots
Pigmentation
Varieties
Anthocyanins
Zanahoria Morada
Purple Carrots
topic Zanahoria
Pigmentación
Variedades
Daucus carota
Antocianinas
Carrots
Pigmentation
Varieties
Anthocyanins
Zanahoria Morada
Purple Carrots
dc.description.none.fl_txt_mv The accumulation of anthocyanin pigments can exhibit different patterns across plant tissues and crop varieties. This variability allowed the investigation of the molecular mechanisms behind the biosynthesis of these pigments in several plant species. Among crops, carrots have a well-defined anthocyanin pigmentation pattern depending on the genic background. In this work, we report on the discovery of DNA structural differences affecting the activity of an R2R3 MYB (encoded by DcMYB11) involved in anthocyanin regulation in carrot petiole. To this end, we first verified the function of DcMYB11 using heterologous systems and identified three different alleles which may explain differences in petiole pigmentation. Characterization of the DcMYB11 alleles at the 5' upstream sequence unveiled a sequence that functions as a putative enhancer. In conclusion, this study provides novel insight into the molecular mechanisms controlling anthocyanin accumulation in carrot. By these outcomes, we expanded our knowledge on the cis-regulatory sequences in plants.
EEA La Consulta
Fil: D’Amelia, Vincenzo. National Research Council. Institute of Bioscience and BioResources; Italia
Fil: D’Amelia, Vincenzo. University of Naples Federico II. Department of Agricultural Sciences; Italia
Fil: Curaba, Julien. North Carolina State University. Horticulture Science Department. Plants for Human Health Institute; Estados Unidos
Fil: Abid, Muhammad Ali. North Carolina State University. Horticulture Science Department. Plants for Human Health Institute; Estados Unidos
Fil: Esposito, Salvatore. CREA Research Centre for Cereal and Industrial Crops; Italia
Fil: Cavagnaro, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Cavagnaro, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina.
Fil: Carputo, Domenico. University of Naples Federico II. Department of Agricultural Sciences; Italia
Fil: Iorizzo, Massimo. North Carolina State University. Horticulture Science Department. Plants for Human Health Institute; Estados Unidos
description The accumulation of anthocyanin pigments can exhibit different patterns across plant tissues and crop varieties. This variability allowed the investigation of the molecular mechanisms behind the biosynthesis of these pigments in several plant species. Among crops, carrots have a well-defined anthocyanin pigmentation pattern depending on the genic background. In this work, we report on the discovery of DNA structural differences affecting the activity of an R2R3 MYB (encoded by DcMYB11) involved in anthocyanin regulation in carrot petiole. To this end, we first verified the function of DcMYB11 using heterologous systems and identified three different alleles which may explain differences in petiole pigmentation. Characterization of the DcMYB11 alleles at the 5' upstream sequence unveiled a sequence that functions as a putative enhancer. In conclusion, this study provides novel insight into the molecular mechanisms controlling anthocyanin accumulation in carrot. By these outcomes, we expanded our knowledge on the cis-regulatory sequences in plants.
publishDate 2023
dc.date.none.fl_str_mv 2023-08-01T11:26:52Z
2023-08-01T11:26:52Z
2023-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/14846
https://link.springer.com/article/10.1007/s00425-023-04200-5
0032-0935
1432-2048
https://doi.org/10.1007/s00425-023-04200-5
url http://hdl.handle.net/20.500.12123/14846
https://link.springer.com/article/10.1007/s00425-023-04200-5
https://doi.org/10.1007/s00425-023-04200-5
identifier_str_mv 0032-0935
1432-2048
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Planta 258 : Article number: 50 (July 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1842975518004084736
score 12.993085

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