Molecular characterization and antimicrobial resistance profiles of Salmonella Heidelberg isolates from poultry

Autores
Dominguez, Johanna Elizabeth; Viñas, María Rosa; Herrera, Mariana; Moroni, Mirian; Gutkind, Gabriel Omar; Mercado, Elsa Cristina; Di Conza, José Alejandro; Chacana, Pablo
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the last 10 years, Salmonella Heidelberg has been extensively isolated from poultry in several countries. In this context, molecular characterization is essential to understand whether the strains have entered the farms from a single or several sources. Thus, the aim of this study was to determine the genetic relationship and antimicrobial susceptibility of S. Heidelberg strains isolated between 2011 and 2012 from broiler farms belonging to three integrated poultry companies located in Argentina. The genetic relatedness of the S. Heidelberg isolates was determined by pulsed-field gel electrophoresis (PFGE), and resistance to 21 antimicrobials was determined by the disc diffusion method. The isolates were assigned to four PFGE patterns. Most of the strains showed 100% similarity and belonged to the same integrated poultry company. This PFGE pattern was also prevalent in S. Heidelberg strains isolated from humans in several provinces of Argentina, which suggests an epidemiological association between human and poultry strains. All the isolates were classified as multidrug-resistant (MDR), and no clear relationship was observed between PFGE and resistance patterns. S. Heidelberg strains may circulate among farms from the same integrated company due to common sources of contamination. To guarantee the safety of the poultry product for the consumers, holistic approaches including surveillance of Salmonella throughout the production chain together with control measures are crucial.
Instituto de Patobiología
Fil: Dominguez, Johana Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Dominguez, Johana Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Viñas, María Rosa. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán (ANLIS-Malbrán). Servicios de Enterobacterias; Argentina
Fil: Herrera, Mariana. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA). Dirección General de Laboratorios y Control Técnico; Argentina
Fil: Moroni, Mirian. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán (ANLIS-Malbrán). Servicios de Enterobacterias; Argentina
Fil: Gutkind, Gabriel O. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; Argentina
Fil: Gutkind, Gabriel O. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mercado, Elsa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Di Conza, José A. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; Argentina
Fil: Di Conza, José A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Chacana, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Zoonoses and Public Health 68 (4) : 309-315 (June 2021)
Materia
Aves de Corral
Salmonella
Genética Molecular
Resistencia a los Antimicrobianos
Poultry
Molecular Genetics
Antimicrobial Resistance
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10883
Acceder
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oai_identifier_str oai:localhost:20.500.12123/10883
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Molecular characterization and antimicrobial resistance profiles of Salmonella Heidelberg isolates from poultryDominguez, Johanna ElizabethViñas, María RosaHerrera, MarianaMoroni, MirianGutkind, Gabriel OmarMercado, Elsa CristinaDi Conza, José AlejandroChacana, PabloAves de CorralSalmonellaGenética MolecularResistencia a los AntimicrobianosPoultryMolecular GeneticsAntimicrobial ResistanceIn the last 10 years, Salmonella Heidelberg has been extensively isolated from poultry in several countries. In this context, molecular characterization is essential to understand whether the strains have entered the farms from a single or several sources. Thus, the aim of this study was to determine the genetic relationship and antimicrobial susceptibility of S. Heidelberg strains isolated between 2011 and 2012 from broiler farms belonging to three integrated poultry companies located in Argentina. The genetic relatedness of the S. Heidelberg isolates was determined by pulsed-field gel electrophoresis (PFGE), and resistance to 21 antimicrobials was determined by the disc diffusion method. The isolates were assigned to four PFGE patterns. Most of the strains showed 100% similarity and belonged to the same integrated poultry company. This PFGE pattern was also prevalent in S. Heidelberg strains isolated from humans in several provinces of Argentina, which suggests an epidemiological association between human and poultry strains. All the isolates were classified as multidrug-resistant (MDR), and no clear relationship was observed between PFGE and resistance patterns. S. Heidelberg strains may circulate among farms from the same integrated company due to common sources of contamination. To guarantee the safety of the poultry product for the consumers, holistic approaches including surveillance of Salmonella throughout the production chain together with control measures are crucial.Instituto de PatobiologíaFil: Dominguez, Johana Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.Fil: Dominguez, Johana Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Viñas, María Rosa. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán (ANLIS-Malbrán). Servicios de Enterobacterias; ArgentinaFil: Herrera, Mariana. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA). Dirección General de Laboratorios y Control Técnico; ArgentinaFil: Moroni, Mirian. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán (ANLIS-Malbrán). Servicios de Enterobacterias; ArgentinaFil: Gutkind, Gabriel O. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; ArgentinaFil: Gutkind, Gabriel O. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mercado, Elsa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Di Conza, José A. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; ArgentinaFil: Di Conza, José A. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Chacana, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley2021-12-10T14:17:42Z2021-12-10T14:17:42Z2021-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10883https://onlinelibrary.wiley.com/doi/10.1111/zph.128191863-2378https://doi.org/10.1111/zph.12819Zoonoses and Public Health 68 (4) : 309-315 (June 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:49:12Zoai:localhost:20.500.12123/10883instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:12.89INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Molecular characterization and antimicrobial resistance profiles of Salmonella Heidelberg isolates from poultry
title Molecular characterization and antimicrobial resistance profiles of Salmonella Heidelberg isolates from poultry
spellingShingle Molecular characterization and antimicrobial resistance profiles of Salmonella Heidelberg isolates from poultry
Dominguez, Johanna Elizabeth
Aves de Corral
Salmonella
Genética Molecular
Resistencia a los Antimicrobianos
Poultry
Molecular Genetics
Antimicrobial Resistance
title_short Molecular characterization and antimicrobial resistance profiles of Salmonella Heidelberg isolates from poultry
title_full Molecular characterization and antimicrobial resistance profiles of Salmonella Heidelberg isolates from poultry
title_fullStr Molecular characterization and antimicrobial resistance profiles of Salmonella Heidelberg isolates from poultry
title_full_unstemmed Molecular characterization and antimicrobial resistance profiles of Salmonella Heidelberg isolates from poultry
title_sort Molecular characterization and antimicrobial resistance profiles of Salmonella Heidelberg isolates from poultry
dc.creator.none.fl_str_mv Dominguez, Johanna Elizabeth
Viñas, María Rosa
Herrera, Mariana
Moroni, Mirian
Gutkind, Gabriel Omar
Mercado, Elsa Cristina
Di Conza, José Alejandro
Chacana, Pablo
author Dominguez, Johanna Elizabeth
author_facet Dominguez, Johanna Elizabeth
Viñas, María Rosa
Herrera, Mariana
Moroni, Mirian
Gutkind, Gabriel Omar
Mercado, Elsa Cristina
Di Conza, José Alejandro
Chacana, Pablo
author_role author
author2 Viñas, María Rosa
Herrera, Mariana
Moroni, Mirian
Gutkind, Gabriel Omar
Mercado, Elsa Cristina
Di Conza, José Alejandro
Chacana, Pablo
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Aves de Corral
Salmonella
Genética Molecular
Resistencia a los Antimicrobianos
Poultry
Molecular Genetics
Antimicrobial Resistance
topic Aves de Corral
Salmonella
Genética Molecular
Resistencia a los Antimicrobianos
Poultry
Molecular Genetics
Antimicrobial Resistance
dc.description.none.fl_txt_mv In the last 10 years, Salmonella Heidelberg has been extensively isolated from poultry in several countries. In this context, molecular characterization is essential to understand whether the strains have entered the farms from a single or several sources. Thus, the aim of this study was to determine the genetic relationship and antimicrobial susceptibility of S. Heidelberg strains isolated between 2011 and 2012 from broiler farms belonging to three integrated poultry companies located in Argentina. The genetic relatedness of the S. Heidelberg isolates was determined by pulsed-field gel electrophoresis (PFGE), and resistance to 21 antimicrobials was determined by the disc diffusion method. The isolates were assigned to four PFGE patterns. Most of the strains showed 100% similarity and belonged to the same integrated poultry company. This PFGE pattern was also prevalent in S. Heidelberg strains isolated from humans in several provinces of Argentina, which suggests an epidemiological association between human and poultry strains. All the isolates were classified as multidrug-resistant (MDR), and no clear relationship was observed between PFGE and resistance patterns. S. Heidelberg strains may circulate among farms from the same integrated company due to common sources of contamination. To guarantee the safety of the poultry product for the consumers, holistic approaches including surveillance of Salmonella throughout the production chain together with control measures are crucial.
Instituto de Patobiología
Fil: Dominguez, Johana Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Dominguez, Johana Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Viñas, María Rosa. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán (ANLIS-Malbrán). Servicios de Enterobacterias; Argentina
Fil: Herrera, Mariana. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA). Dirección General de Laboratorios y Control Técnico; Argentina
Fil: Moroni, Mirian. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos G. Malbrán (ANLIS-Malbrán). Servicios de Enterobacterias; Argentina
Fil: Gutkind, Gabriel O. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; Argentina
Fil: Gutkind, Gabriel O. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mercado, Elsa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Di Conza, José A. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones en Bacteriología y Virología Molecular; Argentina
Fil: Di Conza, José A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Chacana, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description In the last 10 years, Salmonella Heidelberg has been extensively isolated from poultry in several countries. In this context, molecular characterization is essential to understand whether the strains have entered the farms from a single or several sources. Thus, the aim of this study was to determine the genetic relationship and antimicrobial susceptibility of S. Heidelberg strains isolated between 2011 and 2012 from broiler farms belonging to three integrated poultry companies located in Argentina. The genetic relatedness of the S. Heidelberg isolates was determined by pulsed-field gel electrophoresis (PFGE), and resistance to 21 antimicrobials was determined by the disc diffusion method. The isolates were assigned to four PFGE patterns. Most of the strains showed 100% similarity and belonged to the same integrated poultry company. This PFGE pattern was also prevalent in S. Heidelberg strains isolated from humans in several provinces of Argentina, which suggests an epidemiological association between human and poultry strains. All the isolates were classified as multidrug-resistant (MDR), and no clear relationship was observed between PFGE and resistance patterns. S. Heidelberg strains may circulate among farms from the same integrated company due to common sources of contamination. To guarantee the safety of the poultry product for the consumers, holistic approaches including surveillance of Salmonella throughout the production chain together with control measures are crucial.
publishDate 2021
dc.date.none.fl_str_mv 2021-12-10T14:17:42Z
2021-12-10T14:17:42Z
2021-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10883
https://onlinelibrary.wiley.com/doi/10.1111/zph.12819
1863-2378
https://doi.org/10.1111/zph.12819
url http://hdl.handle.net/20.500.12123/10883
https://onlinelibrary.wiley.com/doi/10.1111/zph.12819
https://doi.org/10.1111/zph.12819
identifier_str_mv 1863-2378
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Zoonoses and Public Health 68 (4) : 309-315 (June 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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