Simultaneous carriage of mcr-1 and other antimicrobial resistance determinants in Escherichia coli from poultry

Autores
Dominguez, Johanna Elizabeth; Redondo, Leandro Martín; Figueroa Espinosa, Roque A.; Cejas, Daniela; Gutkind, Gabriel Omar; Chacana, Pablo; Di Conza, José Alejandro; Fernandez Miyakawa, Mariano Enrique
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The use of antimicrobial growth promoters (AGPs) in sub-therapeutic doses for long periods promotes the selection of resistant microorganisms and the subsequent risk of spreading this resistance to the human population and the environment. Global concern about antimicrobial resistance development and transference of resistance genes from animal to human has been rising. The goal of our research was to evaluate the susceptibility pattern to different classes of antimicrobials of colistin-resistant Escherichia coli from poultry production systems that use AGPs, and characterize the resistance determinants associated to transferable platforms. E. coli strains (n = 41) were obtained from fecal samples collected from typical Argentine commercial broiler farms and susceptibility for 23 antimicrobials, relevant for human or veterinary medicine, was determined. Isolates were tested by PCR for the presence of mcr-1, extended spectrum β-lactamase encoding genes and plasmid-mediated quinolone resistance (PMQR) coding genes. Conjugation and susceptibility patterns of the transconjugant studies were performed. ERIC-PCR and REP-PCR analysis showed a high diversity of the isolates. Resistance to several antimicrobials was determined and all colistin-resistant isolates harbored the mcr-1 gene. CTX-M-2 cefotaximase was the main mechanism responsible for third generation cephalosporins resistance, and PMQR determinants were also identified. In addition, co-transference of the qnrB determinant on the mcr-1-positive transconjugants was corroborated, which suggests that these resistance genes are likely to be located in the same plasmid. In this work a wide range of antimicrobial resistance mechanisms were identified in E. coli strains isolated from the environment of healthy chickens highlighting the risk of antimicrobial abuse/misuse in animals under intensive production systems and its consequences for public health.
Instituto de Patobiología
Fil: Dominguez, Johana Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Redondo, Leandro Martí­n. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Figueroa Espinosa, Roque A. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Resistencia Bacteriana. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cejas, Daniela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Resistencia Bacteriana. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gutkind, Gabriel O. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Resistencia Bacteriana. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Di Conza, José A. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Resistencia Bacteriana. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernandez Miyakawa, Mariano Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Frontiers in Microbiology 25 (9): 1679 (Julio 2018)
Materia
Colistin
Foodborne Diseases
Drug Resistance
Poultry
Colistina
Enfermedades Transmitidas por Alimentos
Escherichia coli
Resistencia a Medicamentos
Aves de Corral
Antimicrobial Resistance
Resistencia Antimicrobiana
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3774
Acceder
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network_name_str INTA Digital (INTA)
spelling Simultaneous carriage of mcr-1 and other antimicrobial resistance determinants in Escherichia coli from poultryDominguez, Johanna ElizabethRedondo, Leandro MartínFigueroa Espinosa, Roque A.Cejas, DanielaGutkind, Gabriel OmarChacana, PabloDi Conza, José AlejandroFernandez Miyakawa, Mariano EnriqueColistinFoodborne DiseasesDrug ResistancePoultryColistinaEnfermedades Transmitidas por AlimentosEscherichia coliResistencia a MedicamentosAves de CorralAntimicrobial ResistanceResistencia AntimicrobianaThe use of antimicrobial growth promoters (AGPs) in sub-therapeutic doses for long periods promotes the selection of resistant microorganisms and the subsequent risk of spreading this resistance to the human population and the environment. Global concern about antimicrobial resistance development and transference of resistance genes from animal to human has been rising. The goal of our research was to evaluate the susceptibility pattern to different classes of antimicrobials of colistin-resistant Escherichia coli from poultry production systems that use AGPs, and characterize the resistance determinants associated to transferable platforms. E. coli strains (n = 41) were obtained from fecal samples collected from typical Argentine commercial broiler farms and susceptibility for 23 antimicrobials, relevant for human or veterinary medicine, was determined. Isolates were tested by PCR for the presence of mcr-1, extended spectrum β-lactamase encoding genes and plasmid-mediated quinolone resistance (PMQR) coding genes. Conjugation and susceptibility patterns of the transconjugant studies were performed. ERIC-PCR and REP-PCR analysis showed a high diversity of the isolates. Resistance to several antimicrobials was determined and all colistin-resistant isolates harbored the mcr-1 gene. CTX-M-2 cefotaximase was the main mechanism responsible for third generation cephalosporins resistance, and PMQR determinants were also identified. In addition, co-transference of the qnrB determinant on the mcr-1-positive transconjugants was corroborated, which suggests that these resistance genes are likely to be located in the same plasmid. In this work a wide range of antimicrobial resistance mechanisms were identified in E. coli strains isolated from the environment of healthy chickens highlighting the risk of antimicrobial abuse/misuse in animals under intensive production systems and its consequences for public health.Instituto de PatobiologíaFil: Dominguez, Johana Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Redondo, Leandro Martí­n. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Figueroa Espinosa, Roque A. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Resistencia Bacteriana. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cejas, Daniela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Resistencia Bacteriana. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gutkind, Gabriel O. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Resistencia Bacteriana. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Di Conza, José A. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Resistencia Bacteriana. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fernandez Miyakawa, Mariano Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFrontiers Media2018-11-02T17:01:55Z2018-11-02T17:01:55Z2018-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/3774https://www.frontiersin.org/articles/10.3389/fmicb.2018.01679/full1664-302Xhttps://doi.org/10.3389/fmicb.2018.01679Frontiers in Microbiology 25 (9): 1679 (Julio 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNSA/1115056/AR./Enfermedades infecciosas de las aves.info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:47:38Zoai:localhost:20.500.12123/3774instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:39.717INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Simultaneous carriage of mcr-1 and other antimicrobial resistance determinants in Escherichia coli from poultry
title Simultaneous carriage of mcr-1 and other antimicrobial resistance determinants in Escherichia coli from poultry
spellingShingle Simultaneous carriage of mcr-1 and other antimicrobial resistance determinants in Escherichia coli from poultry
Dominguez, Johanna Elizabeth
Colistin
Foodborne Diseases
Drug Resistance
Poultry
Colistina
Enfermedades Transmitidas por Alimentos
Escherichia coli
Resistencia a Medicamentos
Aves de Corral
Antimicrobial Resistance
Resistencia Antimicrobiana
title_short Simultaneous carriage of mcr-1 and other antimicrobial resistance determinants in Escherichia coli from poultry
title_full Simultaneous carriage of mcr-1 and other antimicrobial resistance determinants in Escherichia coli from poultry
title_fullStr Simultaneous carriage of mcr-1 and other antimicrobial resistance determinants in Escherichia coli from poultry
title_full_unstemmed Simultaneous carriage of mcr-1 and other antimicrobial resistance determinants in Escherichia coli from poultry
title_sort Simultaneous carriage of mcr-1 and other antimicrobial resistance determinants in Escherichia coli from poultry
dc.creator.none.fl_str_mv Dominguez, Johanna Elizabeth
Redondo, Leandro Martín
Figueroa Espinosa, Roque A.
Cejas, Daniela
Gutkind, Gabriel Omar
Chacana, Pablo
Di Conza, José Alejandro
Fernandez Miyakawa, Mariano Enrique
author Dominguez, Johanna Elizabeth
author_facet Dominguez, Johanna Elizabeth
Redondo, Leandro Martín
Figueroa Espinosa, Roque A.
Cejas, Daniela
Gutkind, Gabriel Omar
Chacana, Pablo
Di Conza, José Alejandro
Fernandez Miyakawa, Mariano Enrique
author_role author
author2 Redondo, Leandro Martín
Figueroa Espinosa, Roque A.
Cejas, Daniela
Gutkind, Gabriel Omar
Chacana, Pablo
Di Conza, José Alejandro
Fernandez Miyakawa, Mariano Enrique
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Colistin
Foodborne Diseases
Drug Resistance
Poultry
Colistina
Enfermedades Transmitidas por Alimentos
Escherichia coli
Resistencia a Medicamentos
Aves de Corral
Antimicrobial Resistance
Resistencia Antimicrobiana
topic Colistin
Foodborne Diseases
Drug Resistance
Poultry
Colistina
Enfermedades Transmitidas por Alimentos
Escherichia coli
Resistencia a Medicamentos
Aves de Corral
Antimicrobial Resistance
Resistencia Antimicrobiana
dc.description.none.fl_txt_mv The use of antimicrobial growth promoters (AGPs) in sub-therapeutic doses for long periods promotes the selection of resistant microorganisms and the subsequent risk of spreading this resistance to the human population and the environment. Global concern about antimicrobial resistance development and transference of resistance genes from animal to human has been rising. The goal of our research was to evaluate the susceptibility pattern to different classes of antimicrobials of colistin-resistant Escherichia coli from poultry production systems that use AGPs, and characterize the resistance determinants associated to transferable platforms. E. coli strains (n = 41) were obtained from fecal samples collected from typical Argentine commercial broiler farms and susceptibility for 23 antimicrobials, relevant for human or veterinary medicine, was determined. Isolates were tested by PCR for the presence of mcr-1, extended spectrum β-lactamase encoding genes and plasmid-mediated quinolone resistance (PMQR) coding genes. Conjugation and susceptibility patterns of the transconjugant studies were performed. ERIC-PCR and REP-PCR analysis showed a high diversity of the isolates. Resistance to several antimicrobials was determined and all colistin-resistant isolates harbored the mcr-1 gene. CTX-M-2 cefotaximase was the main mechanism responsible for third generation cephalosporins resistance, and PMQR determinants were also identified. In addition, co-transference of the qnrB determinant on the mcr-1-positive transconjugants was corroborated, which suggests that these resistance genes are likely to be located in the same plasmid. In this work a wide range of antimicrobial resistance mechanisms were identified in E. coli strains isolated from the environment of healthy chickens highlighting the risk of antimicrobial abuse/misuse in animals under intensive production systems and its consequences for public health.
Instituto de Patobiología
Fil: Dominguez, Johana Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Redondo, Leandro Martí­n. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Figueroa Espinosa, Roque A. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Resistencia Bacteriana. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cejas, Daniela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Resistencia Bacteriana. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gutkind, Gabriel O. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Resistencia Bacteriana. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Di Conza, José A. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Resistencia Bacteriana. Cátedra de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernandez Miyakawa, Mariano Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The use of antimicrobial growth promoters (AGPs) in sub-therapeutic doses for long periods promotes the selection of resistant microorganisms and the subsequent risk of spreading this resistance to the human population and the environment. Global concern about antimicrobial resistance development and transference of resistance genes from animal to human has been rising. The goal of our research was to evaluate the susceptibility pattern to different classes of antimicrobials of colistin-resistant Escherichia coli from poultry production systems that use AGPs, and characterize the resistance determinants associated to transferable platforms. E. coli strains (n = 41) were obtained from fecal samples collected from typical Argentine commercial broiler farms and susceptibility for 23 antimicrobials, relevant for human or veterinary medicine, was determined. Isolates were tested by PCR for the presence of mcr-1, extended spectrum β-lactamase encoding genes and plasmid-mediated quinolone resistance (PMQR) coding genes. Conjugation and susceptibility patterns of the transconjugant studies were performed. ERIC-PCR and REP-PCR analysis showed a high diversity of the isolates. Resistance to several antimicrobials was determined and all colistin-resistant isolates harbored the mcr-1 gene. CTX-M-2 cefotaximase was the main mechanism responsible for third generation cephalosporins resistance, and PMQR determinants were also identified. In addition, co-transference of the qnrB determinant on the mcr-1-positive transconjugants was corroborated, which suggests that these resistance genes are likely to be located in the same plasmid. In this work a wide range of antimicrobial resistance mechanisms were identified in E. coli strains isolated from the environment of healthy chickens highlighting the risk of antimicrobial abuse/misuse in animals under intensive production systems and its consequences for public health.
publishDate 2018
dc.date.none.fl_str_mv 2018-11-02T17:01:55Z
2018-11-02T17:01:55Z
2018-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/3774
https://www.frontiersin.org/articles/10.3389/fmicb.2018.01679/full
1664-302X
https://doi.org/10.3389/fmicb.2018.01679
url http://hdl.handle.net/20.500.12123/3774
https://www.frontiersin.org/articles/10.3389/fmicb.2018.01679/full
https://doi.org/10.3389/fmicb.2018.01679
identifier_str_mv 1664-302X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNSA/1115056/AR./Enfermedades infecciosas de las aves.
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Frontiers in Microbiology 25 (9): 1679 (Julio 2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
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instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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