Genomic study of Salmonella ser. Enteritidis from poultry farms in Argentina: Epidemiology and antimicrobial resistance in twenty strains

Autores
Hoffmann, Teresa Magali; Soria, Mario; Bueno, Dante Javier; Huberman, Yosef Daniel; Ishida, María; Sauders, Brian; Deiulio, Gregory; Dickey, Alyssa Wiedenmayer; Medina-Santana, José Luis; Vinueza-Burgos, Christian
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The escalating issue of antimicrobial resistance in pathogens transmitted through the food chain poses a significant threat to public health worldwide. Salmonella ser. Enteritidis (SE) remains a predominant cause of foodborne illness. This research aims to elucidate the genetic and antimicrobial resistance profiles of SE strains isolated from Argentine poultry farms. This study used whole genome sequencing to assess the genetic diversity and resistance patterns of 20 SE strains collected from poultry farms in Entre Ríos, Argentina, between January 2020 and February 2021. Phenotypic assays were used to detect resistance to 50 antibiotics across 14 antimicrobial classes. Genes and mutations linked to antimicrobial resistance were pinpointed on both plasmids and chromosomes of the strains. All investigated strains exhibited resistance to nitrofurantoin, and a high percentage showed resistance or intermediate sensitivity to fluoroquinolones. Genomic analysis showed the presence of resistance genes associated with aminoglycosides, β-lactams, and quinolones. The study distinctly categorized the strains into those isolated from broilers and laying hens, highlighting a complex interplay of resistance and diversity within the poultry industry. This shows antimicrobial resistance within foodborne pathogens like SE and the importance of continuous surveillance systems to track the evolution and spread of resistance. The genomic insights from this study not only enhance understanding of the resistance mechanisms but also facilitate tracking the transmission dynamics of SE, which is crucial for developing targeted interventions.
EEA Concepción del Uruguay
Fil: Hoffmann, Teresa Magali. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hoffmann, Teresa Magali. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Hoffmann, Teresa Magali. Universidad Autónoma de Entre Ríos. Sede Basavilbaso. Facultad de Ciencia y Tecnología; Argentina
Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Bueno, Dante Javier. Universidad Autónoma de Entre Ríos. Sede Basavilbaso. Facultad de Ciencia y Tecnología; Argentina
Fil: Huberman, Yosef Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.
Fil: Huberman, Yosef Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
Fil: Ishida, María. New York State Department of Agriculture and Markets. Division of Food Laboratory; Estados Unidos
Fil: Sauders, Brian. New York State Department of Agriculture and Markets. Division of Food Laboratory; Estados Unidos
Fil: Deiulio, Gregory. New York State Department of Agriculture and Markets. Division of Food Laboratory; Estados Unidos
Fil: Dickey, Alyssa Wiedenmayer. New York State Department of Agriculture and Markets. Division of Food Laboratory; Estados Unidos
Fil: Medina-Santana, José Luis. Universidad Central del Ecuador. Facultad de Medicina Veterinaria y Zootecnia. Unidad de Investigación de Enfermedades Transmitidas por Alimentos y Resistencia a los Antimicrobianos (UNIETAR); Ecuador
Fil: Vinueza-Burgos, Christian. Universidad Central del Ecuador. Facultad de Medicina Veterinaria y Zootecnia. Unidad de Investigación de Enfermedades Transmitidas por Alimentos y Resistencia a los Antimicrobianos (UNIETAR); Ecuador
Fuente
Veterinary Microbiology 304 : 110488. (May 2025)
Materia
Salmonella
Salmonella enteritidis
Aviculture
Genomics
Epidemiology
Antimicrobial Resistance
Avicultura
Genómica
Epidemiología
Resistencia a los Antimicrobianos
Argentina
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/22032
Acceder
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oai_identifier_str oai:localhost:20.500.12123/22032
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Genomic study of Salmonella ser. Enteritidis from poultry farms in Argentina: Epidemiology and antimicrobial resistance in twenty strainsHoffmann, Teresa MagaliSoria, MarioBueno, Dante JavierHuberman, Yosef DanielIshida, MaríaSauders, BrianDeiulio, GregoryDickey, Alyssa WiedenmayerMedina-Santana, José LuisVinueza-Burgos, ChristianSalmonellaSalmonella enteritidisAvicultureGenomicsEpidemiologyAntimicrobial ResistanceAviculturaGenómicaEpidemiologíaResistencia a los AntimicrobianosArgentinaThe escalating issue of antimicrobial resistance in pathogens transmitted through the food chain poses a significant threat to public health worldwide. Salmonella ser. Enteritidis (SE) remains a predominant cause of foodborne illness. This research aims to elucidate the genetic and antimicrobial resistance profiles of SE strains isolated from Argentine poultry farms. This study used whole genome sequencing to assess the genetic diversity and resistance patterns of 20 SE strains collected from poultry farms in Entre Ríos, Argentina, between January 2020 and February 2021. Phenotypic assays were used to detect resistance to 50 antibiotics across 14 antimicrobial classes. Genes and mutations linked to antimicrobial resistance were pinpointed on both plasmids and chromosomes of the strains. All investigated strains exhibited resistance to nitrofurantoin, and a high percentage showed resistance or intermediate sensitivity to fluoroquinolones. Genomic analysis showed the presence of resistance genes associated with aminoglycosides, β-lactams, and quinolones. The study distinctly categorized the strains into those isolated from broilers and laying hens, highlighting a complex interplay of resistance and diversity within the poultry industry. This shows antimicrobial resistance within foodborne pathogens like SE and the importance of continuous surveillance systems to track the evolution and spread of resistance. The genomic insights from this study not only enhance understanding of the resistance mechanisms but also facilitate tracking the transmission dynamics of SE, which is crucial for developing targeted interventions.EEA Concepción del UruguayFil: Hoffmann, Teresa Magali. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hoffmann, Teresa Magali. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Hoffmann, Teresa Magali. Universidad Autónoma de Entre Ríos. Sede Basavilbaso. Facultad de Ciencia y Tecnología; ArgentinaFil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; ArgentinaFil: Bueno, Dante Javier. Universidad Autónoma de Entre Ríos. Sede Basavilbaso. Facultad de Ciencia y Tecnología; ArgentinaFil: Huberman, Yosef Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.Fil: Huberman, Yosef Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; ArgentinaFil: Ishida, María. New York State Department of Agriculture and Markets. Division of Food Laboratory; Estados UnidosFil: Sauders, Brian. New York State Department of Agriculture and Markets. Division of Food Laboratory; Estados UnidosFil: Deiulio, Gregory. New York State Department of Agriculture and Markets. Division of Food Laboratory; Estados UnidosFil: Dickey, Alyssa Wiedenmayer. New York State Department of Agriculture and Markets. Division of Food Laboratory; Estados UnidosFil: Medina-Santana, José Luis. Universidad Central del Ecuador. Facultad de Medicina Veterinaria y Zootecnia. Unidad de Investigación de Enfermedades Transmitidas por Alimentos y Resistencia a los Antimicrobianos (UNIETAR); EcuadorFil: Vinueza-Burgos, Christian. Universidad Central del Ecuador. Facultad de Medicina Veterinaria y Zootecnia. Unidad de Investigación de Enfermedades Transmitidas por Alimentos y Resistencia a los Antimicrobianos (UNIETAR); EcuadorElsevier2025-04-24T11:15:23Z2025-04-24T11:15:23Z2025-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/22032https://www.sciencedirect.com/science/article/abs/pii/S03781135250012330378-11351873-2542https://doi.org/10.1016/j.vetmic.2025.110488Veterinary Microbiology 304 : 110488. (May 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L06-I114, Desarrollo y aplicación de métodos diagnósticos y epidemiológicos para la producción pecuaria sustentable y agroalimentaria en humanos con foco en Una Saludinfo:eu-repograntAgreement/INTA/2023-PD-L06-I115, Resistencia Antimicrobiana y desarrollo de alternativas que minimicen el uso de antibióticos y antiparasitarios para una produccion animal mas sustentableinfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:51:01Zoai:localhost:20.500.12123/22032instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:51:01.688INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Genomic study of Salmonella ser. Enteritidis from poultry farms in Argentina: Epidemiology and antimicrobial resistance in twenty strains
title Genomic study of Salmonella ser. Enteritidis from poultry farms in Argentina: Epidemiology and antimicrobial resistance in twenty strains
spellingShingle Genomic study of Salmonella ser. Enteritidis from poultry farms in Argentina: Epidemiology and antimicrobial resistance in twenty strains
Hoffmann, Teresa Magali
Salmonella
Salmonella enteritidis
Aviculture
Genomics
Epidemiology
Antimicrobial Resistance
Avicultura
Genómica
Epidemiología
Resistencia a los Antimicrobianos
Argentina
title_short Genomic study of Salmonella ser. Enteritidis from poultry farms in Argentina: Epidemiology and antimicrobial resistance in twenty strains
title_full Genomic study of Salmonella ser. Enteritidis from poultry farms in Argentina: Epidemiology and antimicrobial resistance in twenty strains
title_fullStr Genomic study of Salmonella ser. Enteritidis from poultry farms in Argentina: Epidemiology and antimicrobial resistance in twenty strains
title_full_unstemmed Genomic study of Salmonella ser. Enteritidis from poultry farms in Argentina: Epidemiology and antimicrobial resistance in twenty strains
title_sort Genomic study of Salmonella ser. Enteritidis from poultry farms in Argentina: Epidemiology and antimicrobial resistance in twenty strains
dc.creator.none.fl_str_mv Hoffmann, Teresa Magali
Soria, Mario
Bueno, Dante Javier
Huberman, Yosef Daniel
Ishida, María
Sauders, Brian
Deiulio, Gregory
Dickey, Alyssa Wiedenmayer
Medina-Santana, José Luis
Vinueza-Burgos, Christian
author Hoffmann, Teresa Magali
author_facet Hoffmann, Teresa Magali
Soria, Mario
Bueno, Dante Javier
Huberman, Yosef Daniel
Ishida, María
Sauders, Brian
Deiulio, Gregory
Dickey, Alyssa Wiedenmayer
Medina-Santana, José Luis
Vinueza-Burgos, Christian
author_role author
author2 Soria, Mario
Bueno, Dante Javier
Huberman, Yosef Daniel
Ishida, María
Sauders, Brian
Deiulio, Gregory
Dickey, Alyssa Wiedenmayer
Medina-Santana, José Luis
Vinueza-Burgos, Christian
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Salmonella
Salmonella enteritidis
Aviculture
Genomics
Epidemiology
Antimicrobial Resistance
Avicultura
Genómica
Epidemiología
Resistencia a los Antimicrobianos
Argentina
topic Salmonella
Salmonella enteritidis
Aviculture
Genomics
Epidemiology
Antimicrobial Resistance
Avicultura
Genómica
Epidemiología
Resistencia a los Antimicrobianos
Argentina
dc.description.none.fl_txt_mv The escalating issue of antimicrobial resistance in pathogens transmitted through the food chain poses a significant threat to public health worldwide. Salmonella ser. Enteritidis (SE) remains a predominant cause of foodborne illness. This research aims to elucidate the genetic and antimicrobial resistance profiles of SE strains isolated from Argentine poultry farms. This study used whole genome sequencing to assess the genetic diversity and resistance patterns of 20 SE strains collected from poultry farms in Entre Ríos, Argentina, between January 2020 and February 2021. Phenotypic assays were used to detect resistance to 50 antibiotics across 14 antimicrobial classes. Genes and mutations linked to antimicrobial resistance were pinpointed on both plasmids and chromosomes of the strains. All investigated strains exhibited resistance to nitrofurantoin, and a high percentage showed resistance or intermediate sensitivity to fluoroquinolones. Genomic analysis showed the presence of resistance genes associated with aminoglycosides, β-lactams, and quinolones. The study distinctly categorized the strains into those isolated from broilers and laying hens, highlighting a complex interplay of resistance and diversity within the poultry industry. This shows antimicrobial resistance within foodborne pathogens like SE and the importance of continuous surveillance systems to track the evolution and spread of resistance. The genomic insights from this study not only enhance understanding of the resistance mechanisms but also facilitate tracking the transmission dynamics of SE, which is crucial for developing targeted interventions.
EEA Concepción del Uruguay
Fil: Hoffmann, Teresa Magali. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hoffmann, Teresa Magali. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Hoffmann, Teresa Magali. Universidad Autónoma de Entre Ríos. Sede Basavilbaso. Facultad de Ciencia y Tecnología; Argentina
Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina
Fil: Bueno, Dante Javier. Universidad Autónoma de Entre Ríos. Sede Basavilbaso. Facultad de Ciencia y Tecnología; Argentina
Fil: Huberman, Yosef Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.
Fil: Huberman, Yosef Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
Fil: Ishida, María. New York State Department of Agriculture and Markets. Division of Food Laboratory; Estados Unidos
Fil: Sauders, Brian. New York State Department of Agriculture and Markets. Division of Food Laboratory; Estados Unidos
Fil: Deiulio, Gregory. New York State Department of Agriculture and Markets. Division of Food Laboratory; Estados Unidos
Fil: Dickey, Alyssa Wiedenmayer. New York State Department of Agriculture and Markets. Division of Food Laboratory; Estados Unidos
Fil: Medina-Santana, José Luis. Universidad Central del Ecuador. Facultad de Medicina Veterinaria y Zootecnia. Unidad de Investigación de Enfermedades Transmitidas por Alimentos y Resistencia a los Antimicrobianos (UNIETAR); Ecuador
Fil: Vinueza-Burgos, Christian. Universidad Central del Ecuador. Facultad de Medicina Veterinaria y Zootecnia. Unidad de Investigación de Enfermedades Transmitidas por Alimentos y Resistencia a los Antimicrobianos (UNIETAR); Ecuador
description The escalating issue of antimicrobial resistance in pathogens transmitted through the food chain poses a significant threat to public health worldwide. Salmonella ser. Enteritidis (SE) remains a predominant cause of foodborne illness. This research aims to elucidate the genetic and antimicrobial resistance profiles of SE strains isolated from Argentine poultry farms. This study used whole genome sequencing to assess the genetic diversity and resistance patterns of 20 SE strains collected from poultry farms in Entre Ríos, Argentina, between January 2020 and February 2021. Phenotypic assays were used to detect resistance to 50 antibiotics across 14 antimicrobial classes. Genes and mutations linked to antimicrobial resistance were pinpointed on both plasmids and chromosomes of the strains. All investigated strains exhibited resistance to nitrofurantoin, and a high percentage showed resistance or intermediate sensitivity to fluoroquinolones. Genomic analysis showed the presence of resistance genes associated with aminoglycosides, β-lactams, and quinolones. The study distinctly categorized the strains into those isolated from broilers and laying hens, highlighting a complex interplay of resistance and diversity within the poultry industry. This shows antimicrobial resistance within foodborne pathogens like SE and the importance of continuous surveillance systems to track the evolution and spread of resistance. The genomic insights from this study not only enhance understanding of the resistance mechanisms but also facilitate tracking the transmission dynamics of SE, which is crucial for developing targeted interventions.
publishDate 2025
dc.date.none.fl_str_mv 2025-04-24T11:15:23Z
2025-04-24T11:15:23Z
2025-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/22032
https://www.sciencedirect.com/science/article/abs/pii/S0378113525001233
0378-1135
1873-2542
https://doi.org/10.1016/j.vetmic.2025.110488
url http://hdl.handle.net/20.500.12123/22032
https://www.sciencedirect.com/science/article/abs/pii/S0378113525001233
https://doi.org/10.1016/j.vetmic.2025.110488
identifier_str_mv 0378-1135
1873-2542
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L06-I114, Desarrollo y aplicación de métodos diagnósticos y epidemiológicos para la producción pecuaria sustentable y agroalimentaria en humanos con foco en Una Salud
info:eu-repograntAgreement/INTA/2023-PD-L06-I115, Resistencia Antimicrobiana y desarrollo de alternativas que minimicen el uso de antibióticos y antiparasitarios para una produccion animal mas sustentable
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary Microbiology 304 : 110488. (May 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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