Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function and carbohydrate metabolism of cassava plants

Autores
Zanini, Andrea Alejandra; Di Feo, Liliana Del Valle; Luna, Dario Fernando; Paccioretti, Pablo; Collavino, Agostina; Rodriguez, Marianela
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cassava common mosaic virus (CsCMV) is a potexvirus that causes systemic infections in cassava plants, leading to chlorotic mosaic and producing significant yield losses. To date, the physiological alterations and the mechanism underlying biotic stress during the cassava–CsCMV compatible interaction remains unknown. In this study, we found that CsCMV infection adversely modified chloroplast structure and had functional effects on chloroplasts in source leaves during the course of viral infection. Extrusion of the chloroplast membrane with amoeboid-shaped appearance and disorganized grana stacks were observed in infected mesophyll cells. These alterations were associated with up to 35% reduction of relative chlorophyll content, and a decline of CO2 fixation (13.5% and 24.2% at 90 and 210 days after planting, respectively). The effects of CsCMV infection on the performance index on absorption basis dropped up to 37%. The analysis of chlorophyll a fluorescence showed a progressive loss of both oxygen evolving complex activity and “connectivity” within the tripartite system (core antenna-LHCII-reaction centre). Here, we report the latter phenomenon for the first time in a viral infection. The oxidative stress process was observed in CsCMV-infected plants (20.8% reduction of antioxidant capacity with respect to noninfected plants). Other effects of the pathogen included reduction of starch and maltose content in source leaves, and a significant increase (24.7%) of the sucrose:starch ratio, which indicates an altered pattern of carbon allocation. Our results suggest that CsCMV induces chloroplast distortion associated with progressive chloroplast function loss and diversion of carbon flux in source leaf tissue, leading to the loss of cassava tuber yield.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Zanini, Andrea Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Zanini, Andrea Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Di Feo, Liliana Del Valle. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Di Feo, Liliana Del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Luna, Dario Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Luna, Dario Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Paccioretti, Pablo. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra de Estadística y Biometría; Argentina
Fil: Collavino, Agostina. Instituto Universitario de Formosa. Facultad de la Producción y del Medio Ambiente. Centro de Investigación y Transferencia de Formosa; Argentina
Fil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Rodriguez, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fuente
Plant Pathology 70 (1) : 195-205 (January 2021)
Materia
Biotic Stress
Cassava
Potexviruses
Estrés Biótico
Mandioca
Cloroplasto
Potexvirus
Chloroplast Alteration
CsCMV
OJIP Test
Potexvirus
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/9664
Acceder
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oai_identifier_str oai:localhost:20.500.12123/9664
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function and carbohydrate metabolism of cassava plantsZanini, Andrea AlejandraDi Feo, Liliana Del ValleLuna, Dario FernandoPaccioretti, PabloCollavino, AgostinaRodriguez, MarianelaBiotic StressCassavaPotexvirusesEstrés BióticoMandiocaCloroplastoPotexvirusChloroplast AlterationCsCMVOJIP TestPotexvirusCassava common mosaic virus (CsCMV) is a potexvirus that causes systemic infections in cassava plants, leading to chlorotic mosaic and producing significant yield losses. To date, the physiological alterations and the mechanism underlying biotic stress during the cassava–CsCMV compatible interaction remains unknown. In this study, we found that CsCMV infection adversely modified chloroplast structure and had functional effects on chloroplasts in source leaves during the course of viral infection. Extrusion of the chloroplast membrane with amoeboid-shaped appearance and disorganized grana stacks were observed in infected mesophyll cells. These alterations were associated with up to 35% reduction of relative chlorophyll content, and a decline of CO2 fixation (13.5% and 24.2% at 90 and 210 days after planting, respectively). The effects of CsCMV infection on the performance index on absorption basis dropped up to 37%. The analysis of chlorophyll a fluorescence showed a progressive loss of both oxygen evolving complex activity and “connectivity” within the tripartite system (core antenna-LHCII-reaction centre). Here, we report the latter phenomenon for the first time in a viral infection. The oxidative stress process was observed in CsCMV-infected plants (20.8% reduction of antioxidant capacity with respect to noninfected plants). Other effects of the pathogen included reduction of starch and maltose content in source leaves, and a significant increase (24.7%) of the sucrose:starch ratio, which indicates an altered pattern of carbon allocation. Our results suggest that CsCMV induces chloroplast distortion associated with progressive chloroplast function loss and diversion of carbon flux in source leaf tissue, leading to the loss of cassava tuber yield.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Zanini, Andrea Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); ArgentinaFil: Zanini, Andrea Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Di Feo, Liliana Del Valle. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Di Feo, Liliana Del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Luna, Dario Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Luna, Dario Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); ArgentinaFil: Paccioretti, Pablo. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra de Estadística y Biometría; ArgentinaFil: Collavino, Agostina. Instituto Universitario de Formosa. Facultad de la Producción y del Medio Ambiente. Centro de Investigación y Transferencia de Formosa; ArgentinaFil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Rodriguez, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); ArgentinaWiley2021-06-25T16:16:52Z2021-06-25T16:16:52Z2021-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9664https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/ppa.132720032-08621365-3059 (online)https://doi.org/10.1111/ppa.13272Plant Pathology 70 (1) : 195-205 (January 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E4-I085-001/2019-PD-E4-I085-001/AR./Determinación de los mecanismos de resistencia a enfermedades mediante la caracterización de las interacciones moleculares en sistemas planta-patógeno.info:eu-repo/semantics/restrictedAccess2025-09-29T13:45:15Zoai:localhost:20.500.12123/9664instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:15.934INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function and carbohydrate metabolism of cassava plants
title Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function and carbohydrate metabolism of cassava plants
spellingShingle Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function and carbohydrate metabolism of cassava plants
Zanini, Andrea Alejandra
Biotic Stress
Cassava
Potexviruses
Estrés Biótico
Mandioca
Cloroplasto
Potexvirus
Chloroplast Alteration
CsCMV
OJIP Test
Potexvirus
title_short Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function and carbohydrate metabolism of cassava plants
title_full Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function and carbohydrate metabolism of cassava plants
title_fullStr Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function and carbohydrate metabolism of cassava plants
title_full_unstemmed Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function and carbohydrate metabolism of cassava plants
title_sort Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function and carbohydrate metabolism of cassava plants
dc.creator.none.fl_str_mv Zanini, Andrea Alejandra
Di Feo, Liliana Del Valle
Luna, Dario Fernando
Paccioretti, Pablo
Collavino, Agostina
Rodriguez, Marianela
author Zanini, Andrea Alejandra
author_facet Zanini, Andrea Alejandra
Di Feo, Liliana Del Valle
Luna, Dario Fernando
Paccioretti, Pablo
Collavino, Agostina
Rodriguez, Marianela
author_role author
author2 Di Feo, Liliana Del Valle
Luna, Dario Fernando
Paccioretti, Pablo
Collavino, Agostina
Rodriguez, Marianela
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Biotic Stress
Cassava
Potexviruses
Estrés Biótico
Mandioca
Cloroplasto
Potexvirus
Chloroplast Alteration
CsCMV
OJIP Test
Potexvirus
topic Biotic Stress
Cassava
Potexviruses
Estrés Biótico
Mandioca
Cloroplasto
Potexvirus
Chloroplast Alteration
CsCMV
OJIP Test
Potexvirus
dc.description.none.fl_txt_mv Cassava common mosaic virus (CsCMV) is a potexvirus that causes systemic infections in cassava plants, leading to chlorotic mosaic and producing significant yield losses. To date, the physiological alterations and the mechanism underlying biotic stress during the cassava–CsCMV compatible interaction remains unknown. In this study, we found that CsCMV infection adversely modified chloroplast structure and had functional effects on chloroplasts in source leaves during the course of viral infection. Extrusion of the chloroplast membrane with amoeboid-shaped appearance and disorganized grana stacks were observed in infected mesophyll cells. These alterations were associated with up to 35% reduction of relative chlorophyll content, and a decline of CO2 fixation (13.5% and 24.2% at 90 and 210 days after planting, respectively). The effects of CsCMV infection on the performance index on absorption basis dropped up to 37%. The analysis of chlorophyll a fluorescence showed a progressive loss of both oxygen evolving complex activity and “connectivity” within the tripartite system (core antenna-LHCII-reaction centre). Here, we report the latter phenomenon for the first time in a viral infection. The oxidative stress process was observed in CsCMV-infected plants (20.8% reduction of antioxidant capacity with respect to noninfected plants). Other effects of the pathogen included reduction of starch and maltose content in source leaves, and a significant increase (24.7%) of the sucrose:starch ratio, which indicates an altered pattern of carbon allocation. Our results suggest that CsCMV induces chloroplast distortion associated with progressive chloroplast function loss and diversion of carbon flux in source leaf tissue, leading to the loss of cassava tuber yield.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Zanini, Andrea Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Zanini, Andrea Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Di Feo, Liliana Del Valle. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Di Feo, Liliana Del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Luna, Dario Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Luna, Dario Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Paccioretti, Pablo. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra de Estadística y Biometría; Argentina
Fil: Collavino, Agostina. Instituto Universitario de Formosa. Facultad de la Producción y del Medio Ambiente. Centro de Investigación y Transferencia de Formosa; Argentina
Fil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Rodriguez, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
description Cassava common mosaic virus (CsCMV) is a potexvirus that causes systemic infections in cassava plants, leading to chlorotic mosaic and producing significant yield losses. To date, the physiological alterations and the mechanism underlying biotic stress during the cassava–CsCMV compatible interaction remains unknown. In this study, we found that CsCMV infection adversely modified chloroplast structure and had functional effects on chloroplasts in source leaves during the course of viral infection. Extrusion of the chloroplast membrane with amoeboid-shaped appearance and disorganized grana stacks were observed in infected mesophyll cells. These alterations were associated with up to 35% reduction of relative chlorophyll content, and a decline of CO2 fixation (13.5% and 24.2% at 90 and 210 days after planting, respectively). The effects of CsCMV infection on the performance index on absorption basis dropped up to 37%. The analysis of chlorophyll a fluorescence showed a progressive loss of both oxygen evolving complex activity and “connectivity” within the tripartite system (core antenna-LHCII-reaction centre). Here, we report the latter phenomenon for the first time in a viral infection. The oxidative stress process was observed in CsCMV-infected plants (20.8% reduction of antioxidant capacity with respect to noninfected plants). Other effects of the pathogen included reduction of starch and maltose content in source leaves, and a significant increase (24.7%) of the sucrose:starch ratio, which indicates an altered pattern of carbon allocation. Our results suggest that CsCMV induces chloroplast distortion associated with progressive chloroplast function loss and diversion of carbon flux in source leaf tissue, leading to the loss of cassava tuber yield.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T16:16:52Z
2021-06-25T16:16:52Z
2021-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/9664
https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/ppa.13272
0032-0862
1365-3059 (online)
https://doi.org/10.1111/ppa.13272
url http://hdl.handle.net/20.500.12123/9664
https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/ppa.13272
https://doi.org/10.1111/ppa.13272
identifier_str_mv 0032-0862
1365-3059 (online)
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E4-I085-001/2019-PD-E4-I085-001/AR./Determinación de los mecanismos de resistencia a enfermedades mediante la caracterización de las interacciones moleculares en sistemas planta-patógeno.
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Plant Pathology 70 (1) : 195-205 (January 2021)
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instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
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instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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