Exploring sugar allocation and metabolic shifts in cassava plants infected with Cassava common mosaic virus (CsCMV) under long-day photoperiod: diel changes in source and sink leav...

Autores
Zanini, Andrea Alejandra; Dominguez Muñoz, Martin Ceferino; Rodriguez, Marianela
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cassava common mosaic virus (CsCMV) is a potexvirus that impairs chloroplast and metabolism, causing significant yield losses to cassava crops. Crop yield depends on diel rhythms, influencing carbon allocation and growth, and sugar signaling also impacting light–dark rhythms. This study aimed to elucidate the early impact of CsCMV infection on diel carbon allocation, metabolism, and defense mechanisms in both source and sink cassava leaves before storage root bulking. Soluble sugar and starch concentrations were examined over a 24-h cycle (16:8 photoperiod) in CsCMV-infected plants. The expression of an array of genes—carbohydrate metabolism, SnRK1 activity marker, defense, circadian marker—was analyzed at ZT6, ZT16 and ZT24/ZT0. In CsCMV-infected source leaves, at ZT6, sucrose increased whereas glucose, fructose and sucrose rose at night. An increase in Suc:hexose ratio and upregulation of SnRK1 activity marker genes and PR1 transcripts were found in infected leaves, suggesting a combination of altered carbon metabolism and defense response mechanisms against the viral infection. GIGANTEA, a clock-controlled gene, showed a reduced expression in infected leaves at ZT6 and ZT24/ ZT0, suggesting a circadian phase shift compared with uninfected control plants. Additionally, starch mobilization transcripts were downregulated at ZT24/ZT0, though starch content remained unchanged during the 24-h cycle. In sink leaves, a transient peak of maltose (ZT6) was observed. Our findings suggest that CsCMV disrupts the plant's natural rhythms of sugar metabolism and allocation. Spikes in sucrose levels may serve as infection signals in the internal daily clock of the plant, influencing plant responses during the cassava-CsCMV interaction.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Zanini, Andrea Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Unidad de Estudios Agropecuarios; Argentina
Fil: Zanini, Andrea Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Unidad de Estudios Agropecuarios; Argentina
Fil: Zanini, Andrea Alejandra. Donald Danforth Plant Science Center; Estados Unidos
Fil: Dominguez Muñoz, Martin Ceferino. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cerro Azul; Argentina
Fil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Unidad de Estudios Agropecuarios; Argentina
Fil: Rodriguez, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Unidad de Estudios Agropecuarios; Argentina
Fil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fuente
Journal of Plant Research 138 : 131–145 (2025)
Materia
Mandioca
Relaciones Fuente Sumidero
Fotoperiodismo
Cassava
Source-sink Relations
Photoperiodicity
CsCMV
Plant-virus interaction
Sugar Allocation
Sugar Signal
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/21051
Acceder
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oai_identifier_str oai:localhost:20.500.12123/21051
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Exploring sugar allocation and metabolic shifts in cassava plants infected with Cassava common mosaic virus (CsCMV) under long-day photoperiod: diel changes in source and sink leavesZanini, Andrea AlejandraDominguez Muñoz, Martin CeferinoRodriguez, MarianelaMandiocaRelaciones Fuente SumideroFotoperiodismoCassavaSource-sink RelationsPhotoperiodicityCsCMVPlant-virus interactionSugar AllocationSugar SignalCassava common mosaic virus (CsCMV) is a potexvirus that impairs chloroplast and metabolism, causing significant yield losses to cassava crops. Crop yield depends on diel rhythms, influencing carbon allocation and growth, and sugar signaling also impacting light–dark rhythms. This study aimed to elucidate the early impact of CsCMV infection on diel carbon allocation, metabolism, and defense mechanisms in both source and sink cassava leaves before storage root bulking. Soluble sugar and starch concentrations were examined over a 24-h cycle (16:8 photoperiod) in CsCMV-infected plants. The expression of an array of genes—carbohydrate metabolism, SnRK1 activity marker, defense, circadian marker—was analyzed at ZT6, ZT16 and ZT24/ZT0. In CsCMV-infected source leaves, at ZT6, sucrose increased whereas glucose, fructose and sucrose rose at night. An increase in Suc:hexose ratio and upregulation of SnRK1 activity marker genes and PR1 transcripts were found in infected leaves, suggesting a combination of altered carbon metabolism and defense response mechanisms against the viral infection. GIGANTEA, a clock-controlled gene, showed a reduced expression in infected leaves at ZT6 and ZT24/ ZT0, suggesting a circadian phase shift compared with uninfected control plants. Additionally, starch mobilization transcripts were downregulated at ZT24/ZT0, though starch content remained unchanged during the 24-h cycle. In sink leaves, a transient peak of maltose (ZT6) was observed. Our findings suggest that CsCMV disrupts the plant's natural rhythms of sugar metabolism and allocation. Spikes in sucrose levels may serve as infection signals in the internal daily clock of the plant, influencing plant responses during the cassava-CsCMV interaction.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Zanini, Andrea Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Unidad de Estudios Agropecuarios; ArgentinaFil: Zanini, Andrea Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Unidad de Estudios Agropecuarios; ArgentinaFil: Zanini, Andrea Alejandra. Donald Danforth Plant Science Center; Estados UnidosFil: Dominguez Muñoz, Martin Ceferino. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cerro Azul; ArgentinaFil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Unidad de Estudios Agropecuarios; ArgentinaFil: Rodriguez, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Unidad de Estudios Agropecuarios; ArgentinaFil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaSpringer2025-01-22T14:33:40Z2025-01-22T14:33:40Z2025-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21051https://link.springer.com/article/10.1007/s10265-024-01595-40918-94401618-0860 (online)https://doi.org/10.1007/s10265-024-01595-4Journal of Plant Research 138 : 131–145 (2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E4-I085-001/2019-PD-E4-I085-001/AR./Determinación de los mecanismos de resistencia a enfermedades mediante la caracterización de las interacciones moleculares en sistemas planta-patógeno.info:eu-repograntAgreement/INTA/2023-PD-L03-I084, Estreses bióticos y abióticos en plantas. Estudios fisiológicos y patológicos para el diseño de estrategias de mejoramiento y manejoinfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-30T11:25:08Zoai:localhost:20.500.12123/21051instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-30 11:25:08.71INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Exploring sugar allocation and metabolic shifts in cassava plants infected with Cassava common mosaic virus (CsCMV) under long-day photoperiod: diel changes in source and sink leaves
title Exploring sugar allocation and metabolic shifts in cassava plants infected with Cassava common mosaic virus (CsCMV) under long-day photoperiod: diel changes in source and sink leaves
spellingShingle Exploring sugar allocation and metabolic shifts in cassava plants infected with Cassava common mosaic virus (CsCMV) under long-day photoperiod: diel changes in source and sink leaves
Zanini, Andrea Alejandra
Mandioca
Relaciones Fuente Sumidero
Fotoperiodismo
Cassava
Source-sink Relations
Photoperiodicity
CsCMV
Plant-virus interaction
Sugar Allocation
Sugar Signal
title_short Exploring sugar allocation and metabolic shifts in cassava plants infected with Cassava common mosaic virus (CsCMV) under long-day photoperiod: diel changes in source and sink leaves
title_full Exploring sugar allocation and metabolic shifts in cassava plants infected with Cassava common mosaic virus (CsCMV) under long-day photoperiod: diel changes in source and sink leaves
title_fullStr Exploring sugar allocation and metabolic shifts in cassava plants infected with Cassava common mosaic virus (CsCMV) under long-day photoperiod: diel changes in source and sink leaves
title_full_unstemmed Exploring sugar allocation and metabolic shifts in cassava plants infected with Cassava common mosaic virus (CsCMV) under long-day photoperiod: diel changes in source and sink leaves
title_sort Exploring sugar allocation and metabolic shifts in cassava plants infected with Cassava common mosaic virus (CsCMV) under long-day photoperiod: diel changes in source and sink leaves
dc.creator.none.fl_str_mv Zanini, Andrea Alejandra
Dominguez Muñoz, Martin Ceferino
Rodriguez, Marianela
author Zanini, Andrea Alejandra
author_facet Zanini, Andrea Alejandra
Dominguez Muñoz, Martin Ceferino
Rodriguez, Marianela
author_role author
author2 Dominguez Muñoz, Martin Ceferino
Rodriguez, Marianela
author2_role author
author
dc.subject.none.fl_str_mv Mandioca
Relaciones Fuente Sumidero
Fotoperiodismo
Cassava
Source-sink Relations
Photoperiodicity
CsCMV
Plant-virus interaction
Sugar Allocation
Sugar Signal
topic Mandioca
Relaciones Fuente Sumidero
Fotoperiodismo
Cassava
Source-sink Relations
Photoperiodicity
CsCMV
Plant-virus interaction
Sugar Allocation
Sugar Signal
dc.description.none.fl_txt_mv Cassava common mosaic virus (CsCMV) is a potexvirus that impairs chloroplast and metabolism, causing significant yield losses to cassava crops. Crop yield depends on diel rhythms, influencing carbon allocation and growth, and sugar signaling also impacting light–dark rhythms. This study aimed to elucidate the early impact of CsCMV infection on diel carbon allocation, metabolism, and defense mechanisms in both source and sink cassava leaves before storage root bulking. Soluble sugar and starch concentrations were examined over a 24-h cycle (16:8 photoperiod) in CsCMV-infected plants. The expression of an array of genes—carbohydrate metabolism, SnRK1 activity marker, defense, circadian marker—was analyzed at ZT6, ZT16 and ZT24/ZT0. In CsCMV-infected source leaves, at ZT6, sucrose increased whereas glucose, fructose and sucrose rose at night. An increase in Suc:hexose ratio and upregulation of SnRK1 activity marker genes and PR1 transcripts were found in infected leaves, suggesting a combination of altered carbon metabolism and defense response mechanisms against the viral infection. GIGANTEA, a clock-controlled gene, showed a reduced expression in infected leaves at ZT6 and ZT24/ ZT0, suggesting a circadian phase shift compared with uninfected control plants. Additionally, starch mobilization transcripts were downregulated at ZT24/ZT0, though starch content remained unchanged during the 24-h cycle. In sink leaves, a transient peak of maltose (ZT6) was observed. Our findings suggest that CsCMV disrupts the plant's natural rhythms of sugar metabolism and allocation. Spikes in sucrose levels may serve as infection signals in the internal daily clock of the plant, influencing plant responses during the cassava-CsCMV interaction.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Zanini, Andrea Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Unidad de Estudios Agropecuarios; Argentina
Fil: Zanini, Andrea Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Unidad de Estudios Agropecuarios; Argentina
Fil: Zanini, Andrea Alejandra. Donald Danforth Plant Science Center; Estados Unidos
Fil: Dominguez Muñoz, Martin Ceferino. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cerro Azul; Argentina
Fil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Unidad de Estudios Agropecuarios; Argentina
Fil: Rodriguez, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Unidad de Estudios Agropecuarios; Argentina
Fil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
description Cassava common mosaic virus (CsCMV) is a potexvirus that impairs chloroplast and metabolism, causing significant yield losses to cassava crops. Crop yield depends on diel rhythms, influencing carbon allocation and growth, and sugar signaling also impacting light–dark rhythms. This study aimed to elucidate the early impact of CsCMV infection on diel carbon allocation, metabolism, and defense mechanisms in both source and sink cassava leaves before storage root bulking. Soluble sugar and starch concentrations were examined over a 24-h cycle (16:8 photoperiod) in CsCMV-infected plants. The expression of an array of genes—carbohydrate metabolism, SnRK1 activity marker, defense, circadian marker—was analyzed at ZT6, ZT16 and ZT24/ZT0. In CsCMV-infected source leaves, at ZT6, sucrose increased whereas glucose, fructose and sucrose rose at night. An increase in Suc:hexose ratio and upregulation of SnRK1 activity marker genes and PR1 transcripts were found in infected leaves, suggesting a combination of altered carbon metabolism and defense response mechanisms against the viral infection. GIGANTEA, a clock-controlled gene, showed a reduced expression in infected leaves at ZT6 and ZT24/ ZT0, suggesting a circadian phase shift compared with uninfected control plants. Additionally, starch mobilization transcripts were downregulated at ZT24/ZT0, though starch content remained unchanged during the 24-h cycle. In sink leaves, a transient peak of maltose (ZT6) was observed. Our findings suggest that CsCMV disrupts the plant's natural rhythms of sugar metabolism and allocation. Spikes in sucrose levels may serve as infection signals in the internal daily clock of the plant, influencing plant responses during the cassava-CsCMV interaction.
publishDate 2025
dc.date.none.fl_str_mv 2025-01-22T14:33:40Z
2025-01-22T14:33:40Z
2025-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/21051
https://link.springer.com/article/10.1007/s10265-024-01595-4
0918-9440
1618-0860 (online)
https://doi.org/10.1007/s10265-024-01595-4
url http://hdl.handle.net/20.500.12123/21051
https://link.springer.com/article/10.1007/s10265-024-01595-4
https://doi.org/10.1007/s10265-024-01595-4
identifier_str_mv 0918-9440
1618-0860 (online)
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E4-I085-001/2019-PD-E4-I085-001/AR./Determinación de los mecanismos de resistencia a enfermedades mediante la caracterización de las interacciones moleculares en sistemas planta-patógeno.
info:eu-repograntAgreement/INTA/2023-PD-L03-I084, Estreses bióticos y abióticos en plantas. Estudios fisiológicos y patológicos para el diseño de estrategias de mejoramiento y manejo
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Journal of Plant Research 138 : 131–145 (2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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