Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function, and carbohydrate metabolism of cassava plants

Autores
Zanini, Andrea Alejandra; Di Feo, Liliana del Valle; Luna, Dario Fernando; Paccioretti, Pablo Ariel; Collavino, Agostina Antonella; Rodriguez, Marianela Soledad
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cassava common mosaic virus (CsCMV) is a potexvirus that causes systemic infections in cassava plants, leading to chlorotic mosaic and producing significant yield losses. To date, the physiological alterations and the mechanism underlying biotic stress during the cassava–CsCMV compatible interaction remains unknown. In this study, we found that CsCMV infection adversely modified chloroplast structure and had functional effects on chloroplasts in source leaves during the course of viral infection. Extrusion of the chloroplast membrane with amoeboid-shaped appearance and disorganized grana stacks were observed in infected mesophyll cells. These alterations were associated with up to 35% reduction of relative chlorophyll content, and a decline of CO2 fixation (13.5% and 24.2% at 90 and 210 days after planting, respectively). The effects of CsCMV infection on the performance index on absorption basis dropped up to 37%. The analysis of chlorophyll a fluorescence showed a progressive loss of both oxygen evolving complex activity and “connectivity” within the tripartite system (core antenna-LHCII-reaction centre). Here, we report the latter phenomenon for the first time in a viral infection. The oxidative stress process was observed in CsCMV-infected plants (20.8% reduction of antioxidant capacity with respect to noninfected plants). Other effects of the pathogen included reduction of starch and maltose content in source leaves, and a significant increase (24.7%) of the sucrose:starch ratio, which indicates an altered pattern of carbon allocation. Our results suggest that CsCMV induces chloroplast distortion associated with progressive chloroplast function loss and diversion of carbon flux in source leaf tissue, leading to the loss of cassava tuber yield.
Fil: Zanini, Andrea Alejandra. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Estudios Agropecuarios.; Argentina
Fil: Di Feo, Liliana del Valle. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatología y Modelización Agrícola - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Fitopatología y Modelización Agrícola; Argentina
Fil: Luna, Dario Fernando. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Estudios Agropecuarios.; Argentina
Fil: Paccioretti, Pablo Ariel. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. Grupo Vinculado Catedra de Estadistica y Biometria de la Facultad de Ciencias Agropecuarias de la Universidad Nacional de Cordoba Al Ufyma | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola. Grupo Vinculado Catedra de Estadistica y Biometria de la Facultad de Ciencias Agropecuarias de la Universidad Nacional de Cordoba Al Ufyma.; Argentina
Fil: Collavino, Agostina Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Formosa. Provincia de Formosa. Centro de Investigaciones y Transferencia de Formosa. Universidad Nacional de Formosa. Centro de Investigaciones y Transferencia de Formosa; Argentina
Fil: Rodriguez, Marianela Soledad. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Estudios Agropecuarios.; Argentina
Materia
BIOTIC STRESS
CASSAVA
CHLOROPLAST ALTERATION
CSCMV
OJIP TEST
POTEXVIRUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/131408
Acceder
id CONICETDig_acef22a5c3447bf96ea981baffa359ff
oai_identifier_str oai:ri.conicet.gov.ar:11336/131408
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function, and carbohydrate metabolism of cassava plantsZanini, Andrea AlejandraDi Feo, Liliana del ValleLuna, Dario FernandoPaccioretti, Pablo ArielCollavino, Agostina AntonellaRodriguez, Marianela SoledadBIOTIC STRESSCASSAVACHLOROPLAST ALTERATIONCSCMVOJIP TESTPOTEXVIRUShttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Cassava common mosaic virus (CsCMV) is a potexvirus that causes systemic infections in cassava plants, leading to chlorotic mosaic and producing significant yield losses. To date, the physiological alterations and the mechanism underlying biotic stress during the cassava–CsCMV compatible interaction remains unknown. In this study, we found that CsCMV infection adversely modified chloroplast structure and had functional effects on chloroplasts in source leaves during the course of viral infection. Extrusion of the chloroplast membrane with amoeboid-shaped appearance and disorganized grana stacks were observed in infected mesophyll cells. These alterations were associated with up to 35% reduction of relative chlorophyll content, and a decline of CO2 fixation (13.5% and 24.2% at 90 and 210 days after planting, respectively). The effects of CsCMV infection on the performance index on absorption basis dropped up to 37%. The analysis of chlorophyll a fluorescence showed a progressive loss of both oxygen evolving complex activity and “connectivity” within the tripartite system (core antenna-LHCII-reaction centre). Here, we report the latter phenomenon for the first time in a viral infection. The oxidative stress process was observed in CsCMV-infected plants (20.8% reduction of antioxidant capacity with respect to noninfected plants). Other effects of the pathogen included reduction of starch and maltose content in source leaves, and a significant increase (24.7%) of the sucrose:starch ratio, which indicates an altered pattern of carbon allocation. Our results suggest that CsCMV induces chloroplast distortion associated with progressive chloroplast function loss and diversion of carbon flux in source leaf tissue, leading to the loss of cassava tuber yield.Fil: Zanini, Andrea Alejandra. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Estudios Agropecuarios.; ArgentinaFil: Di Feo, Liliana del Valle. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatología y Modelización Agrícola - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Fitopatología y Modelización Agrícola; ArgentinaFil: Luna, Dario Fernando. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Estudios Agropecuarios.; ArgentinaFil: Paccioretti, Pablo Ariel. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. Grupo Vinculado Catedra de Estadistica y Biometria de la Facultad de Ciencias Agropecuarias de la Universidad Nacional de Cordoba Al Ufyma | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola. Grupo Vinculado Catedra de Estadistica y Biometria de la Facultad de Ciencias Agropecuarias de la Universidad Nacional de Cordoba Al Ufyma.; ArgentinaFil: Collavino, Agostina Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Formosa. Provincia de Formosa. Centro de Investigaciones y Transferencia de Formosa. Universidad Nacional de Formosa. Centro de Investigaciones y Transferencia de Formosa; ArgentinaFil: Rodriguez, Marianela Soledad. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Estudios Agropecuarios.; ArgentinaWiley Blackwell Publishing, Inc2021-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/131408Zanini, Andrea Alejandra; Di Feo, Liliana del Valle; Luna, Dario Fernando; Paccioretti, Pablo Ariel; Collavino, Agostina Antonella; et al.; Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function, and carbohydrate metabolism of cassava plants; Wiley Blackwell Publishing, Inc; Plant Pathology; 70; 1; 1-2021; 195-2050032-0862CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/ppa.13272info:eu-repo/semantics/altIdentifier/doi/10.1111/ppa.13272info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:32:32Zoai:ri.conicet.gov.ar:11336/131408instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:32:32.362CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function, and carbohydrate metabolism of cassava plants
title Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function, and carbohydrate metabolism of cassava plants
spellingShingle Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function, and carbohydrate metabolism of cassava plants
Zanini, Andrea Alejandra
BIOTIC STRESS
CASSAVA
CHLOROPLAST ALTERATION
CSCMV
OJIP TEST
POTEXVIRUS
title_short Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function, and carbohydrate metabolism of cassava plants
title_full Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function, and carbohydrate metabolism of cassava plants
title_fullStr Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function, and carbohydrate metabolism of cassava plants
title_full_unstemmed Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function, and carbohydrate metabolism of cassava plants
title_sort Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function, and carbohydrate metabolism of cassava plants
dc.creator.none.fl_str_mv Zanini, Andrea Alejandra
Di Feo, Liliana del Valle
Luna, Dario Fernando
Paccioretti, Pablo Ariel
Collavino, Agostina Antonella
Rodriguez, Marianela Soledad
author Zanini, Andrea Alejandra
author_facet Zanini, Andrea Alejandra
Di Feo, Liliana del Valle
Luna, Dario Fernando
Paccioretti, Pablo Ariel
Collavino, Agostina Antonella
Rodriguez, Marianela Soledad
author_role author
author2 Di Feo, Liliana del Valle
Luna, Dario Fernando
Paccioretti, Pablo Ariel
Collavino, Agostina Antonella
Rodriguez, Marianela Soledad
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BIOTIC STRESS
CASSAVA
CHLOROPLAST ALTERATION
CSCMV
OJIP TEST
POTEXVIRUS
topic BIOTIC STRESS
CASSAVA
CHLOROPLAST ALTERATION
CSCMV
OJIP TEST
POTEXVIRUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Cassava common mosaic virus (CsCMV) is a potexvirus that causes systemic infections in cassava plants, leading to chlorotic mosaic and producing significant yield losses. To date, the physiological alterations and the mechanism underlying biotic stress during the cassava–CsCMV compatible interaction remains unknown. In this study, we found that CsCMV infection adversely modified chloroplast structure and had functional effects on chloroplasts in source leaves during the course of viral infection. Extrusion of the chloroplast membrane with amoeboid-shaped appearance and disorganized grana stacks were observed in infected mesophyll cells. These alterations were associated with up to 35% reduction of relative chlorophyll content, and a decline of CO2 fixation (13.5% and 24.2% at 90 and 210 days after planting, respectively). The effects of CsCMV infection on the performance index on absorption basis dropped up to 37%. The analysis of chlorophyll a fluorescence showed a progressive loss of both oxygen evolving complex activity and “connectivity” within the tripartite system (core antenna-LHCII-reaction centre). Here, we report the latter phenomenon for the first time in a viral infection. The oxidative stress process was observed in CsCMV-infected plants (20.8% reduction of antioxidant capacity with respect to noninfected plants). Other effects of the pathogen included reduction of starch and maltose content in source leaves, and a significant increase (24.7%) of the sucrose:starch ratio, which indicates an altered pattern of carbon allocation. Our results suggest that CsCMV induces chloroplast distortion associated with progressive chloroplast function loss and diversion of carbon flux in source leaf tissue, leading to the loss of cassava tuber yield.
Fil: Zanini, Andrea Alejandra. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Estudios Agropecuarios.; Argentina
Fil: Di Feo, Liliana del Valle. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatología y Modelización Agrícola - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Fitopatología y Modelización Agrícola; Argentina
Fil: Luna, Dario Fernando. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Estudios Agropecuarios.; Argentina
Fil: Paccioretti, Pablo Ariel. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Fitopatologia y Modelizacion Agricola. Grupo Vinculado Catedra de Estadistica y Biometria de la Facultad de Ciencias Agropecuarias de la Universidad Nacional de Cordoba Al Ufyma | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Fitopatologia y Modelizacion Agricola. Grupo Vinculado Catedra de Estadistica y Biometria de la Facultad de Ciencias Agropecuarias de la Universidad Nacional de Cordoba Al Ufyma.; Argentina
Fil: Collavino, Agostina Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Formosa. Provincia de Formosa. Centro de Investigaciones y Transferencia de Formosa. Universidad Nacional de Formosa. Centro de Investigaciones y Transferencia de Formosa; Argentina
Fil: Rodriguez, Marianela Soledad. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Estudios Agropecuarios.; Argentina
description Cassava common mosaic virus (CsCMV) is a potexvirus that causes systemic infections in cassava plants, leading to chlorotic mosaic and producing significant yield losses. To date, the physiological alterations and the mechanism underlying biotic stress during the cassava–CsCMV compatible interaction remains unknown. In this study, we found that CsCMV infection adversely modified chloroplast structure and had functional effects on chloroplasts in source leaves during the course of viral infection. Extrusion of the chloroplast membrane with amoeboid-shaped appearance and disorganized grana stacks were observed in infected mesophyll cells. These alterations were associated with up to 35% reduction of relative chlorophyll content, and a decline of CO2 fixation (13.5% and 24.2% at 90 and 210 days after planting, respectively). The effects of CsCMV infection on the performance index on absorption basis dropped up to 37%. The analysis of chlorophyll a fluorescence showed a progressive loss of both oxygen evolving complex activity and “connectivity” within the tripartite system (core antenna-LHCII-reaction centre). Here, we report the latter phenomenon for the first time in a viral infection. The oxidative stress process was observed in CsCMV-infected plants (20.8% reduction of antioxidant capacity with respect to noninfected plants). Other effects of the pathogen included reduction of starch and maltose content in source leaves, and a significant increase (24.7%) of the sucrose:starch ratio, which indicates an altered pattern of carbon allocation. Our results suggest that CsCMV induces chloroplast distortion associated with progressive chloroplast function loss and diversion of carbon flux in source leaf tissue, leading to the loss of cassava tuber yield.
publishDate 2021
dc.date.none.fl_str_mv 2021-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/131408
Zanini, Andrea Alejandra; Di Feo, Liliana del Valle; Luna, Dario Fernando; Paccioretti, Pablo Ariel; Collavino, Agostina Antonella; et al.; Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function, and carbohydrate metabolism of cassava plants; Wiley Blackwell Publishing, Inc; Plant Pathology; 70; 1; 1-2021; 195-205
0032-0862
CONICET Digital
CONICET
url http://hdl.handle.net/11336/131408
identifier_str_mv Zanini, Andrea Alejandra; Di Feo, Liliana del Valle; Luna, Dario Fernando; Paccioretti, Pablo Ariel; Collavino, Agostina Antonella; et al.; Cassava common mosaic virus infection causes alterations in chloroplast ultrastructure, function, and carbohydrate metabolism of cassava plants; Wiley Blackwell Publishing, Inc; Plant Pathology; 70; 1; 1-2021; 195-205
0032-0862
CONICET Digital
CONICET
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language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1111/ppa.13272
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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