The ammonium channel NOD26 is the evolutionary innovation that drives the emergence, consolidation, and dissemination of nitrogen-fixing symbiosis in angiosperms
- Autores
- Frare, Romina Alejandra; Ayub, Nicolás Daniel; Alleva, Karina Edith; Soto, Gabriela Cinthia
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Increasing evidence indicates that N-fixing symbiosis has evolved several times in the N-fixing clade of angiosperms and that this evolution is driven by a single evolutionary innovation. However, the genetics of this ancestral predisposition to N-fixing symbiosis remains unclear. A natural candidate for such molecular innovation is the ammonium channel NOD26, the main protein component of the symbiosome membrane, which facilitates the plant uptake of the nitrogen fixed by symbiotic bacteria. Here, in concordance with the emergence of N-fixing symbiosis in angiosperms but not in ancestral plants, phylogenetic analysis showed that NOD26 belongs to an angiosperm-exclusive subgroup of aquaporins. Integrated genomic, phylogenetic, and gene expression analyses supported NOD26 occurrence in the N-fixing clade, the increase in the NOD26 copy number by block and tandem duplications in legumes, and the low-copy number or even the loss of NOD26 in non-legume species of the N-fixing clade, which correlated with the possibility to lose N-fixing symbiosis in legume and non-legume lineages. Metabolic reconstructions showed that retention of NOD26 in N-fixing precursor could represent an adaptive mechanism to bypass energy crisis during anaerobic stress by ammonium detoxification. Finally, we discuss the potential use of NOD26 to transfer N-fixation to non-N-fixing crops as cereals.
Instituto de Genética
Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alleva, Karina Edith. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina
Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Fuente
- Journal of Molecular Evolution 86 (8) : 554-565 (Octubre 2018)
- Materia
-
Ammonium
Anaerobic Treatment
Symbiosis
Nitrogen Fixation
Angiosperms
Evolution
Amonio
Tratamiento Anaeróbico
Simbiosis
Fijación del Nitrógeno
Angiospermas
Evolución - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/6703
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The ammonium channel NOD26 is the evolutionary innovation that drives the emergence, consolidation, and dissemination of nitrogen-fixing symbiosis in angiospermsFrare, Romina AlejandraAyub, Nicolás DanielAlleva, Karina EdithSoto, Gabriela CinthiaAmmoniumAnaerobic TreatmentSymbiosisNitrogen FixationAngiospermsEvolutionAmonioTratamiento AnaeróbicoSimbiosisFijación del NitrógenoAngiospermasEvoluciónIncreasing evidence indicates that N-fixing symbiosis has evolved several times in the N-fixing clade of angiosperms and that this evolution is driven by a single evolutionary innovation. However, the genetics of this ancestral predisposition to N-fixing symbiosis remains unclear. A natural candidate for such molecular innovation is the ammonium channel NOD26, the main protein component of the symbiosome membrane, which facilitates the plant uptake of the nitrogen fixed by symbiotic bacteria. Here, in concordance with the emergence of N-fixing symbiosis in angiosperms but not in ancestral plants, phylogenetic analysis showed that NOD26 belongs to an angiosperm-exclusive subgroup of aquaporins. Integrated genomic, phylogenetic, and gene expression analyses supported NOD26 occurrence in the N-fixing clade, the increase in the NOD26 copy number by block and tandem duplications in legumes, and the low-copy number or even the loss of NOD26 in non-legume species of the N-fixing clade, which correlated with the possibility to lose N-fixing symbiosis in legume and non-legume lineages. Metabolic reconstructions showed that retention of NOD26 in N-fixing precursor could represent an adaptive mechanism to bypass energy crisis during anaerobic stress by ammonium detoxification. Finally, we discuss the potential use of NOD26 to transfer N-fixation to non-N-fixing crops as cereals.Instituto de GenéticaFil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alleva, Karina Edith. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; ArgentinaFil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer2020-02-10T13:44:08Z2020-02-10T13:44:08Z2018-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/6703https://link.springer.com/article/10.1007%2Fs00239-018-9867-31432-1432https://doi.org/10.1007/s00239-018-9867-3Journal of Molecular Evolution 86 (8) : 554-565 (Octubre 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:52Zoai:localhost:20.500.12123/6703instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:52.491INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
The ammonium channel NOD26 is the evolutionary innovation that drives the emergence, consolidation, and dissemination of nitrogen-fixing symbiosis in angiosperms |
title |
The ammonium channel NOD26 is the evolutionary innovation that drives the emergence, consolidation, and dissemination of nitrogen-fixing symbiosis in angiosperms |
spellingShingle |
The ammonium channel NOD26 is the evolutionary innovation that drives the emergence, consolidation, and dissemination of nitrogen-fixing symbiosis in angiosperms Frare, Romina Alejandra Ammonium Anaerobic Treatment Symbiosis Nitrogen Fixation Angiosperms Evolution Amonio Tratamiento Anaeróbico Simbiosis Fijación del Nitrógeno Angiospermas Evolución |
title_short |
The ammonium channel NOD26 is the evolutionary innovation that drives the emergence, consolidation, and dissemination of nitrogen-fixing symbiosis in angiosperms |
title_full |
The ammonium channel NOD26 is the evolutionary innovation that drives the emergence, consolidation, and dissemination of nitrogen-fixing symbiosis in angiosperms |
title_fullStr |
The ammonium channel NOD26 is the evolutionary innovation that drives the emergence, consolidation, and dissemination of nitrogen-fixing symbiosis in angiosperms |
title_full_unstemmed |
The ammonium channel NOD26 is the evolutionary innovation that drives the emergence, consolidation, and dissemination of nitrogen-fixing symbiosis in angiosperms |
title_sort |
The ammonium channel NOD26 is the evolutionary innovation that drives the emergence, consolidation, and dissemination of nitrogen-fixing symbiosis in angiosperms |
dc.creator.none.fl_str_mv |
Frare, Romina Alejandra Ayub, Nicolás Daniel Alleva, Karina Edith Soto, Gabriela Cinthia |
author |
Frare, Romina Alejandra |
author_facet |
Frare, Romina Alejandra Ayub, Nicolás Daniel Alleva, Karina Edith Soto, Gabriela Cinthia |
author_role |
author |
author2 |
Ayub, Nicolás Daniel Alleva, Karina Edith Soto, Gabriela Cinthia |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Ammonium Anaerobic Treatment Symbiosis Nitrogen Fixation Angiosperms Evolution Amonio Tratamiento Anaeróbico Simbiosis Fijación del Nitrógeno Angiospermas Evolución |
topic |
Ammonium Anaerobic Treatment Symbiosis Nitrogen Fixation Angiosperms Evolution Amonio Tratamiento Anaeróbico Simbiosis Fijación del Nitrógeno Angiospermas Evolución |
dc.description.none.fl_txt_mv |
Increasing evidence indicates that N-fixing symbiosis has evolved several times in the N-fixing clade of angiosperms and that this evolution is driven by a single evolutionary innovation. However, the genetics of this ancestral predisposition to N-fixing symbiosis remains unclear. A natural candidate for such molecular innovation is the ammonium channel NOD26, the main protein component of the symbiosome membrane, which facilitates the plant uptake of the nitrogen fixed by symbiotic bacteria. Here, in concordance with the emergence of N-fixing symbiosis in angiosperms but not in ancestral plants, phylogenetic analysis showed that NOD26 belongs to an angiosperm-exclusive subgroup of aquaporins. Integrated genomic, phylogenetic, and gene expression analyses supported NOD26 occurrence in the N-fixing clade, the increase in the NOD26 copy number by block and tandem duplications in legumes, and the low-copy number or even the loss of NOD26 in non-legume species of the N-fixing clade, which correlated with the possibility to lose N-fixing symbiosis in legume and non-legume lineages. Metabolic reconstructions showed that retention of NOD26 in N-fixing precursor could represent an adaptive mechanism to bypass energy crisis during anaerobic stress by ammonium detoxification. Finally, we discuss the potential use of NOD26 to transfer N-fixation to non-N-fixing crops as cereals. Instituto de Genética Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Alleva, Karina Edith. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
Increasing evidence indicates that N-fixing symbiosis has evolved several times in the N-fixing clade of angiosperms and that this evolution is driven by a single evolutionary innovation. However, the genetics of this ancestral predisposition to N-fixing symbiosis remains unclear. A natural candidate for such molecular innovation is the ammonium channel NOD26, the main protein component of the symbiosome membrane, which facilitates the plant uptake of the nitrogen fixed by symbiotic bacteria. Here, in concordance with the emergence of N-fixing symbiosis in angiosperms but not in ancestral plants, phylogenetic analysis showed that NOD26 belongs to an angiosperm-exclusive subgroup of aquaporins. Integrated genomic, phylogenetic, and gene expression analyses supported NOD26 occurrence in the N-fixing clade, the increase in the NOD26 copy number by block and tandem duplications in legumes, and the low-copy number or even the loss of NOD26 in non-legume species of the N-fixing clade, which correlated with the possibility to lose N-fixing symbiosis in legume and non-legume lineages. Metabolic reconstructions showed that retention of NOD26 in N-fixing precursor could represent an adaptive mechanism to bypass energy crisis during anaerobic stress by ammonium detoxification. Finally, we discuss the potential use of NOD26 to transfer N-fixation to non-N-fixing crops as cereals. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-10 2020-02-10T13:44:08Z 2020-02-10T13:44:08Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/6703 https://link.springer.com/article/10.1007%2Fs00239-018-9867-3 1432-1432 https://doi.org/10.1007/s00239-018-9867-3 |
url |
http://hdl.handle.net/20.500.12123/6703 https://link.springer.com/article/10.1007%2Fs00239-018-9867-3 https://doi.org/10.1007/s00239-018-9867-3 |
identifier_str_mv |
1432-1432 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Journal of Molecular Evolution 86 (8) : 554-565 (Octubre 2018) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
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INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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1844619141733941248 |
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12.559606 |