Metabolic engineering of ammonium release for nitrogen-fixing multispecies microbial cell-factories

Autores
Ortiz Marquez, Juan César Federico; Do Nascimento, Mauro; Curatti, Leonardo
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The biological nitrogen fixation carried out by some Bacteria and Archaea is one of the most attractive alternatives to synthetic nitrogen fertilizers. In this study we compared the effect of controlling the maximum activation state of the Azotobacter vinelandii glutamine synthase by a point mutation at the active site (D49S mutation) and impairing the ammonium-dependent homeostatic control of nitrogen- fixation genes expression by the ΔnifL mutation on ammonium release by the cells. Strains bearing the single D49S mutation were more efficient ammonium producers under carbon/energy limiting conditions and sustained microalgae growth at the expense of atmospheric N2 in synthetic microalgae– bacteria consortia. Ammonium delivery by the different strains had implications for the microalga's cell-size distribution. It was uncovered an extensive cross regulation between nitrogen fixation and assimilation that extends current knowledge on this key metabolic pathway and might represent valuable hints for further improvements of versatile N2-fixing microbial-cell factories.
Fil: Ortiz Marquez, Juan César Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones En Biodiversidad y Biotecnología; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina
Fil: Do Nascimento, Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones En Biodiversidad y Biotecnología; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina
Fil: Curatti, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones En Biodiversidad y Biotecnología; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina
Materia
Nitrogen Fixation
Nitrogen Assimilation
Ammonium Excretion
Fertilizer
Microalgae Biomass
Synthetic Consortia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10755
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Metabolic engineering of ammonium release for nitrogen-fixing multispecies microbial cell-factoriesOrtiz Marquez, Juan César FedericoDo Nascimento, MauroCuratti, LeonardoNitrogen FixationNitrogen AssimilationAmmonium ExcretionFertilizerMicroalgae BiomassSynthetic Consortiahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The biological nitrogen fixation carried out by some Bacteria and Archaea is one of the most attractive alternatives to synthetic nitrogen fertilizers. In this study we compared the effect of controlling the maximum activation state of the Azotobacter vinelandii glutamine synthase by a point mutation at the active site (D49S mutation) and impairing the ammonium-dependent homeostatic control of nitrogen- fixation genes expression by the ΔnifL mutation on ammonium release by the cells. Strains bearing the single D49S mutation were more efficient ammonium producers under carbon/energy limiting conditions and sustained microalgae growth at the expense of atmospheric N2 in synthetic microalgae– bacteria consortia. Ammonium delivery by the different strains had implications for the microalga's cell-size distribution. It was uncovered an extensive cross regulation between nitrogen fixation and assimilation that extends current knowledge on this key metabolic pathway and might represent valuable hints for further improvements of versatile N2-fixing microbial-cell factories.Fil: Ortiz Marquez, Juan César Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones En Biodiversidad y Biotecnología; Argentina. Fundación para Investigaciones Biológicas Aplicadas; ArgentinaFil: Do Nascimento, Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones En Biodiversidad y Biotecnología; Argentina. Fundación para Investigaciones Biológicas Aplicadas; ArgentinaFil: Curatti, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones En Biodiversidad y Biotecnología; Argentina. Fundación para Investigaciones Biológicas Aplicadas; ArgentinaElsevier2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10755Ortiz Marquez, Juan César Federico; Do Nascimento, Mauro; Curatti, Leonardo; Metabolic engineering of ammonium release for nitrogen-fixing multispecies microbial cell-factories; Elsevier; Metabolic Engineering; 23; 3-2014; 154-1641096-7176enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ymben.2014.03.002info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1096717614000366info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:14Zoai:ri.conicet.gov.ar:11336/10755instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:15.218CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Metabolic engineering of ammonium release for nitrogen-fixing multispecies microbial cell-factories
title Metabolic engineering of ammonium release for nitrogen-fixing multispecies microbial cell-factories
spellingShingle Metabolic engineering of ammonium release for nitrogen-fixing multispecies microbial cell-factories
Ortiz Marquez, Juan César Federico
Nitrogen Fixation
Nitrogen Assimilation
Ammonium Excretion
Fertilizer
Microalgae Biomass
Synthetic Consortia
title_short Metabolic engineering of ammonium release for nitrogen-fixing multispecies microbial cell-factories
title_full Metabolic engineering of ammonium release for nitrogen-fixing multispecies microbial cell-factories
title_fullStr Metabolic engineering of ammonium release for nitrogen-fixing multispecies microbial cell-factories
title_full_unstemmed Metabolic engineering of ammonium release for nitrogen-fixing multispecies microbial cell-factories
title_sort Metabolic engineering of ammonium release for nitrogen-fixing multispecies microbial cell-factories
dc.creator.none.fl_str_mv Ortiz Marquez, Juan César Federico
Do Nascimento, Mauro
Curatti, Leonardo
author Ortiz Marquez, Juan César Federico
author_facet Ortiz Marquez, Juan César Federico
Do Nascimento, Mauro
Curatti, Leonardo
author_role author
author2 Do Nascimento, Mauro
Curatti, Leonardo
author2_role author
author
dc.subject.none.fl_str_mv Nitrogen Fixation
Nitrogen Assimilation
Ammonium Excretion
Fertilizer
Microalgae Biomass
Synthetic Consortia
topic Nitrogen Fixation
Nitrogen Assimilation
Ammonium Excretion
Fertilizer
Microalgae Biomass
Synthetic Consortia
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The biological nitrogen fixation carried out by some Bacteria and Archaea is one of the most attractive alternatives to synthetic nitrogen fertilizers. In this study we compared the effect of controlling the maximum activation state of the Azotobacter vinelandii glutamine synthase by a point mutation at the active site (D49S mutation) and impairing the ammonium-dependent homeostatic control of nitrogen- fixation genes expression by the ΔnifL mutation on ammonium release by the cells. Strains bearing the single D49S mutation were more efficient ammonium producers under carbon/energy limiting conditions and sustained microalgae growth at the expense of atmospheric N2 in synthetic microalgae– bacteria consortia. Ammonium delivery by the different strains had implications for the microalga's cell-size distribution. It was uncovered an extensive cross regulation between nitrogen fixation and assimilation that extends current knowledge on this key metabolic pathway and might represent valuable hints for further improvements of versatile N2-fixing microbial-cell factories.
Fil: Ortiz Marquez, Juan César Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones En Biodiversidad y Biotecnología; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina
Fil: Do Nascimento, Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones En Biodiversidad y Biotecnología; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina
Fil: Curatti, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones En Biodiversidad y Biotecnología; Argentina. Fundación para Investigaciones Biológicas Aplicadas; Argentina
description The biological nitrogen fixation carried out by some Bacteria and Archaea is one of the most attractive alternatives to synthetic nitrogen fertilizers. In this study we compared the effect of controlling the maximum activation state of the Azotobacter vinelandii glutamine synthase by a point mutation at the active site (D49S mutation) and impairing the ammonium-dependent homeostatic control of nitrogen- fixation genes expression by the ΔnifL mutation on ammonium release by the cells. Strains bearing the single D49S mutation were more efficient ammonium producers under carbon/energy limiting conditions and sustained microalgae growth at the expense of atmospheric N2 in synthetic microalgae– bacteria consortia. Ammonium delivery by the different strains had implications for the microalga's cell-size distribution. It was uncovered an extensive cross regulation between nitrogen fixation and assimilation that extends current knowledge on this key metabolic pathway and might represent valuable hints for further improvements of versatile N2-fixing microbial-cell factories.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10755
Ortiz Marquez, Juan César Federico; Do Nascimento, Mauro; Curatti, Leonardo; Metabolic engineering of ammonium release for nitrogen-fixing multispecies microbial cell-factories; Elsevier; Metabolic Engineering; 23; 3-2014; 154-164
1096-7176
url http://hdl.handle.net/11336/10755
identifier_str_mv Ortiz Marquez, Juan César Federico; Do Nascimento, Mauro; Curatti, Leonardo; Metabolic engineering of ammonium release for nitrogen-fixing multispecies microbial cell-factories; Elsevier; Metabolic Engineering; 23; 3-2014; 154-164
1096-7176
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ymben.2014.03.002
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1096717614000366
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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