A viral infection reshapes Arabidopsis water management via root hydraulics, 1 aquaporin downregulation and osmotic adjustment

Autores
Manacorda, Carlos Augusto; Cáceres, Pablo D.; Sutka, Moira; Amodeo, Gabriela; Asurmendi, Sebastian; Baroli, Irene
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
The effect of plant viruses on root water relations and on how roots and shoots coordinate under infection remains poorly understood. Using a hydroponic Arabidopsis thaliana–Turnip mosaic virus (TuMV) pathosystem, we integrated biometric, anatomical, hydraulic, and gas-exchange measurements to dissect how viral infection reshapes root–shoot water relations. TuMV impaired root development, as reflected by an early plateau of primary root elongation. At the functional level, infected plants exhibited a decrease in root hydraulic conductance per unit root mass, concomitant with transcriptional downregulation of root aquaporin genes. Despite this, the relative contribution of aquaporin-mediated water transport, assessed via sodium azide inhibition, remained unchanged, indicating that the virus downregulates total hydraulic capacity without altering the apoplastic–symplastic partitioning of water flow. Gas-exchange analysis revealed a virus-induced decoupling between stomatal conductance and net CO₂ assimilation, resulting in a non-adaptive increase in intrinsic water-use efficiency. This loss of photosynthetic plasticity, combined with shoot-localized osmotic adjustment (more negative leaf osmotic potential and higher relative water content), points to a constrained, suboptimal physiological state. Multivariate analysis confirmed that variation in physiological traits largely drives phenotypic divergence between treatments. Together, these coordinated alterations, reduced root hydraulics, rigid gas-exchange relationships and passive hydraulic matching to a stunted shoot, depict plants locked into a low-performance equilibrium, poorly equipped to compete for water and carbon. This work reveals a systemic hydraulic–photosynthetic reconfiguration that could account for compromises in plant resilience and resource competitiveness. Highlight TuMV infection induces a coordinated whole-plant hydraulic reconfiguration characterized by premature growth arrest, reduced root hydraulic conductance, and decoupling of stomatal conductance from photosynthesis, resulting in a constrained physiological state.
Instituto de Biotecnología
Fil: Manacorda, Carlos Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Manacorda, Carlos Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Cáceres, Pablo D. Universidad de Buenos Aires (UBA). Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad, Biología Experimental y Aplicada. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Cáceres, Pablo D. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Sutka, Moira. Universidad de Buenos Aires (UBA). Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad, Biología Experimental y Aplicada. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Sutka, Moira. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Amodeo, Gabriela. Universidad de Buenos Aires (UBA). Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad, Biología Experimental y Aplicada. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Amodeo, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Baroli, Irene. Universidad de Buenos Aires (UBA). Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad, Biología Experimental y Aplicada. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Baroli, Irene. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fuente
BioRxiv : the preprint server for biology (December 16, 2025)
Materia
Arabidopsis thaliana
Conductancia Hidráulica de Raíces
Transpiración
Virus de las Plantas
Root Hydraulic conductivity
Transpiration
Plant Viruses
Aquaporin
Turnip Mosaic Virus
Acuaporina
Virus del Mosaico del Nabo
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/25036
Acceder
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oai_identifier_str oai:localhost:20.500.12123/25036
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling A viral infection reshapes Arabidopsis water management via root hydraulics, 1 aquaporin downregulation and osmotic adjustmentManacorda, Carlos AugustoCáceres, Pablo D.Sutka, MoiraAmodeo, GabrielaAsurmendi, SebastianBaroli, IreneArabidopsis thalianaConductancia Hidráulica de RaícesTranspiraciónVirus de las PlantasRoot Hydraulic conductivityTranspirationPlant VirusesAquaporinTurnip Mosaic VirusAcuaporinaVirus del Mosaico del NaboThe effect of plant viruses on root water relations and on how roots and shoots coordinate under infection remains poorly understood. Using a hydroponic Arabidopsis thaliana–Turnip mosaic virus (TuMV) pathosystem, we integrated biometric, anatomical, hydraulic, and gas-exchange measurements to dissect how viral infection reshapes root–shoot water relations. TuMV impaired root development, as reflected by an early plateau of primary root elongation. At the functional level, infected plants exhibited a decrease in root hydraulic conductance per unit root mass, concomitant with transcriptional downregulation of root aquaporin genes. Despite this, the relative contribution of aquaporin-mediated water transport, assessed via sodium azide inhibition, remained unchanged, indicating that the virus downregulates total hydraulic capacity without altering the apoplastic–symplastic partitioning of water flow. Gas-exchange analysis revealed a virus-induced decoupling between stomatal conductance and net CO₂ assimilation, resulting in a non-adaptive increase in intrinsic water-use efficiency. This loss of photosynthetic plasticity, combined with shoot-localized osmotic adjustment (more negative leaf osmotic potential and higher relative water content), points to a constrained, suboptimal physiological state. Multivariate analysis confirmed that variation in physiological traits largely drives phenotypic divergence between treatments. Together, these coordinated alterations, reduced root hydraulics, rigid gas-exchange relationships and passive hydraulic matching to a stunted shoot, depict plants locked into a low-performance equilibrium, poorly equipped to compete for water and carbon. This work reveals a systemic hydraulic–photosynthetic reconfiguration that could account for compromises in plant resilience and resource competitiveness. Highlight TuMV infection induces a coordinated whole-plant hydraulic reconfiguration characterized by premature growth arrest, reduced root hydraulic conductance, and decoupling of stomatal conductance from photosynthesis, resulting in a constrained physiological state.Instituto de BiotecnologíaFil: Manacorda, Carlos Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Manacorda, Carlos Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Cáceres, Pablo D. Universidad de Buenos Aires (UBA). Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad, Biología Experimental y Aplicada. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Cáceres, Pablo D. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Sutka, Moira. Universidad de Buenos Aires (UBA). Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad, Biología Experimental y Aplicada. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Sutka, Moira. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Amodeo, Gabriela. Universidad de Buenos Aires (UBA). Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad, Biología Experimental y Aplicada. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Amodeo, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Baroli, Irene. Universidad de Buenos Aires (UBA). Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad, Biología Experimental y Aplicada. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Baroli, Irene. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaBioRxiv2026-01-23T13:35:47Z2026-01-23T13:35:47Z2025-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/25036https://www.biorxiv.org/content/10.64898/2025.12.15.694488v1https://doi.org/10.64898/2025.12.15.694488BioRxiv : the preprint server for biology (December 16, 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L03-I084, Estreses bióticos y abióticos en plantas. Estudios fisiológicos y patológicos para el diseño de estrategias de mejoramiento y manejoinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-02-26T11:47:39Zoai:localhost:20.500.12123/25036instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-02-26 11:47:39.857INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv A viral infection reshapes Arabidopsis water management via root hydraulics, 1 aquaporin downregulation and osmotic adjustment
title A viral infection reshapes Arabidopsis water management via root hydraulics, 1 aquaporin downregulation and osmotic adjustment
spellingShingle A viral infection reshapes Arabidopsis water management via root hydraulics, 1 aquaporin downregulation and osmotic adjustment
Manacorda, Carlos Augusto
Arabidopsis thaliana
Conductancia Hidráulica de Raíces
Transpiración
Virus de las Plantas
Root Hydraulic conductivity
Transpiration
Plant Viruses
Aquaporin
Turnip Mosaic Virus
Acuaporina
Virus del Mosaico del Nabo
title_short A viral infection reshapes Arabidopsis water management via root hydraulics, 1 aquaporin downregulation and osmotic adjustment
title_full A viral infection reshapes Arabidopsis water management via root hydraulics, 1 aquaporin downregulation and osmotic adjustment
title_fullStr A viral infection reshapes Arabidopsis water management via root hydraulics, 1 aquaporin downregulation and osmotic adjustment
title_full_unstemmed A viral infection reshapes Arabidopsis water management via root hydraulics, 1 aquaporin downregulation and osmotic adjustment
title_sort A viral infection reshapes Arabidopsis water management via root hydraulics, 1 aquaporin downregulation and osmotic adjustment
dc.creator.none.fl_str_mv Manacorda, Carlos Augusto
Cáceres, Pablo D.
Sutka, Moira
Amodeo, Gabriela
Asurmendi, Sebastian
Baroli, Irene
author Manacorda, Carlos Augusto
author_facet Manacorda, Carlos Augusto
Cáceres, Pablo D.
Sutka, Moira
Amodeo, Gabriela
Asurmendi, Sebastian
Baroli, Irene
author_role author
author2 Cáceres, Pablo D.
Sutka, Moira
Amodeo, Gabriela
Asurmendi, Sebastian
Baroli, Irene
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Arabidopsis thaliana
Conductancia Hidráulica de Raíces
Transpiración
Virus de las Plantas
Root Hydraulic conductivity
Transpiration
Plant Viruses
Aquaporin
Turnip Mosaic Virus
Acuaporina
Virus del Mosaico del Nabo
topic Arabidopsis thaliana
Conductancia Hidráulica de Raíces
Transpiración
Virus de las Plantas
Root Hydraulic conductivity
Transpiration
Plant Viruses
Aquaporin
Turnip Mosaic Virus
Acuaporina
Virus del Mosaico del Nabo
dc.description.none.fl_txt_mv The effect of plant viruses on root water relations and on how roots and shoots coordinate under infection remains poorly understood. Using a hydroponic Arabidopsis thaliana–Turnip mosaic virus (TuMV) pathosystem, we integrated biometric, anatomical, hydraulic, and gas-exchange measurements to dissect how viral infection reshapes root–shoot water relations. TuMV impaired root development, as reflected by an early plateau of primary root elongation. At the functional level, infected plants exhibited a decrease in root hydraulic conductance per unit root mass, concomitant with transcriptional downregulation of root aquaporin genes. Despite this, the relative contribution of aquaporin-mediated water transport, assessed via sodium azide inhibition, remained unchanged, indicating that the virus downregulates total hydraulic capacity without altering the apoplastic–symplastic partitioning of water flow. Gas-exchange analysis revealed a virus-induced decoupling between stomatal conductance and net CO₂ assimilation, resulting in a non-adaptive increase in intrinsic water-use efficiency. This loss of photosynthetic plasticity, combined with shoot-localized osmotic adjustment (more negative leaf osmotic potential and higher relative water content), points to a constrained, suboptimal physiological state. Multivariate analysis confirmed that variation in physiological traits largely drives phenotypic divergence between treatments. Together, these coordinated alterations, reduced root hydraulics, rigid gas-exchange relationships and passive hydraulic matching to a stunted shoot, depict plants locked into a low-performance equilibrium, poorly equipped to compete for water and carbon. This work reveals a systemic hydraulic–photosynthetic reconfiguration that could account for compromises in plant resilience and resource competitiveness. Highlight TuMV infection induces a coordinated whole-plant hydraulic reconfiguration characterized by premature growth arrest, reduced root hydraulic conductance, and decoupling of stomatal conductance from photosynthesis, resulting in a constrained physiological state.
Instituto de Biotecnología
Fil: Manacorda, Carlos Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Manacorda, Carlos Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Cáceres, Pablo D. Universidad de Buenos Aires (UBA). Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad, Biología Experimental y Aplicada. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Cáceres, Pablo D. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Sutka, Moira. Universidad de Buenos Aires (UBA). Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad, Biología Experimental y Aplicada. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Sutka, Moira. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Amodeo, Gabriela. Universidad de Buenos Aires (UBA). Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad, Biología Experimental y Aplicada. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Amodeo, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Baroli, Irene. Universidad de Buenos Aires (UBA). Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad, Biología Experimental y Aplicada. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Baroli, Irene. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
description The effect of plant viruses on root water relations and on how roots and shoots coordinate under infection remains poorly understood. Using a hydroponic Arabidopsis thaliana–Turnip mosaic virus (TuMV) pathosystem, we integrated biometric, anatomical, hydraulic, and gas-exchange measurements to dissect how viral infection reshapes root–shoot water relations. TuMV impaired root development, as reflected by an early plateau of primary root elongation. At the functional level, infected plants exhibited a decrease in root hydraulic conductance per unit root mass, concomitant with transcriptional downregulation of root aquaporin genes. Despite this, the relative contribution of aquaporin-mediated water transport, assessed via sodium azide inhibition, remained unchanged, indicating that the virus downregulates total hydraulic capacity without altering the apoplastic–symplastic partitioning of water flow. Gas-exchange analysis revealed a virus-induced decoupling between stomatal conductance and net CO₂ assimilation, resulting in a non-adaptive increase in intrinsic water-use efficiency. This loss of photosynthetic plasticity, combined with shoot-localized osmotic adjustment (more negative leaf osmotic potential and higher relative water content), points to a constrained, suboptimal physiological state. Multivariate analysis confirmed that variation in physiological traits largely drives phenotypic divergence between treatments. Together, these coordinated alterations, reduced root hydraulics, rigid gas-exchange relationships and passive hydraulic matching to a stunted shoot, depict plants locked into a low-performance equilibrium, poorly equipped to compete for water and carbon. This work reveals a systemic hydraulic–photosynthetic reconfiguration that could account for compromises in plant resilience and resource competitiveness. Highlight TuMV infection induces a coordinated whole-plant hydraulic reconfiguration characterized by premature growth arrest, reduced root hydraulic conductance, and decoupling of stomatal conductance from photosynthesis, resulting in a constrained physiological state.
publishDate 2025
dc.date.none.fl_str_mv 2025-12
2026-01-23T13:35:47Z
2026-01-23T13:35:47Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/25036
https://www.biorxiv.org/content/10.64898/2025.12.15.694488v1
https://doi.org/10.64898/2025.12.15.694488
url http://hdl.handle.net/20.500.12123/25036
https://www.biorxiv.org/content/10.64898/2025.12.15.694488v1
https://doi.org/10.64898/2025.12.15.694488
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L03-I084, Estreses bióticos y abióticos en plantas. Estudios fisiológicos y patológicos para el diseño de estrategias de mejoramiento y manejo
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioRxiv
publisher.none.fl_str_mv BioRxiv
dc.source.none.fl_str_mv BioRxiv : the preprint server for biology (December 16, 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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