Salicylic acid determines differential senescence produced by two turnip mosaic virus strains involving reactive oxygen species and early transcriptomic changes

Autores
Manacorda, Carlos Augusto; Mansilla, Carmen; Debat, Humberto Julio; Zavallo, Diego; Sánchez, Flora; Ponz, Fernando; Asurmendi, Sebastian
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Losses produced by virus diseases depend mostly on symptom severity. Turnip mosaic virus (TuMV) is one of the most damaging and widespread potyvirus infecting members of the family Brassicaceae, including Arabidopsis thaliana. We used JPN1 and UK1 TuMV strains to characterize viral infections regarding symptom development, senescence progression, antioxidant response, reactive oxygen species (ROS) accumulation, and transcriptional profiling. Both isolates, despite accumulating similar viral titers, induced different symptomatology and strong differences in oxidative status. Early differences in several senescenceassociated genes linked to the ORE1 and ORS1 regulatory networks as well as persistent divergence in key ROS production and scavenging systems of the plant were detected. However, at a later stage, both strains induced nutrient competition, indicating that senescence rates are influenced by different mechanisms upon viral infections. Analyses of ORE1 and ORS1 levels in infected Brassica juncea plants showed a similar pattern, suggesting a conserved differential response to both strains in Brassicaceae spp. Transcriptional analysis of the ORE1 and ORS1 regulons showed similarities between salicylic acid (SA) response and the early induction triggered by UK1, the most severe strain. By means of SA-defective NahG transgenic plants, we found that differential senescence progression and ROS accumulation between strains rely on an intact SA pathway.
Fil: Manacorda, Carlos Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Mansilla, Carmen. Universidad Politécnica de Madrid. Centro de Biotecnología y Genómica de Plantas; España
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sánchez, Flora. Universidad Politécnica de Madrid. Centro de Biotecnología y Genómica de Plantas; España
Fil: Ponz, Fernando. Universidad Politécnica de Madrid. Centro de Biotecnología y Genómica de Plantas; España
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Molecular plant-microbe interactions 26 (12) : 1486-1498. (2013)
Materia
Acido Salicílico
Envejecimiento
Nabo
Brassica Juncea
Virus de las Plantas
Plant Viruses
Turnips
Aging
Salicylic Acids
Virus del Mosaico de Nabo
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2184
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2184
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Salicylic acid determines differential senescence produced by two turnip mosaic virus strains involving reactive oxygen species and early transcriptomic changesManacorda, Carlos AugustoMansilla, CarmenDebat, Humberto JulioZavallo, DiegoSánchez, FloraPonz, FernandoAsurmendi, SebastianAcido SalicílicoEnvejecimientoNaboBrassica JunceaVirus de las PlantasPlant VirusesTurnipsAgingSalicylic AcidsVirus del Mosaico de NaboLosses produced by virus diseases depend mostly on symptom severity. Turnip mosaic virus (TuMV) is one of the most damaging and widespread potyvirus infecting members of the family Brassicaceae, including Arabidopsis thaliana. We used JPN1 and UK1 TuMV strains to characterize viral infections regarding symptom development, senescence progression, antioxidant response, reactive oxygen species (ROS) accumulation, and transcriptional profiling. Both isolates, despite accumulating similar viral titers, induced different symptomatology and strong differences in oxidative status. Early differences in several senescenceassociated genes linked to the ORE1 and ORS1 regulatory networks as well as persistent divergence in key ROS production and scavenging systems of the plant were detected. However, at a later stage, both strains induced nutrient competition, indicating that senescence rates are influenced by different mechanisms upon viral infections. Analyses of ORE1 and ORS1 levels in infected Brassica juncea plants showed a similar pattern, suggesting a conserved differential response to both strains in Brassicaceae spp. Transcriptional analysis of the ORE1 and ORS1 regulons showed similarities between salicylic acid (SA) response and the early induction triggered by UK1, the most severe strain. By means of SA-defective NahG transgenic plants, we found that differential senescence progression and ROS accumulation between strains rely on an intact SA pathway.Fil: Manacorda, Carlos Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Mansilla, Carmen. Universidad Politécnica de Madrid. Centro de Biotecnología y Genómica de Plantas; EspañaFil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sánchez, Flora. Universidad Politécnica de Madrid. Centro de Biotecnología y Genómica de Plantas; EspañaFil: Ponz, Fernando. Universidad Politécnica de Madrid. Centro de Biotecnología y Genómica de Plantas; EspañaFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina2018-04-05T18:11:25Z2018-04-05T18:11:25Z2013-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/2184https://apsjournals.apsnet.org/doi/pdf/10.1094/MPMI-07-13-0190-R0894-02821943-7706 (online version)http://dx.doi.org/10.1094/MPMI-07-13-0190-RMolecular plant-microbe interactions 26 (12) : 1486-1498. (2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:16:29Zoai:localhost:20.500.12123/2184instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:16:30.076INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Salicylic acid determines differential senescence produced by two turnip mosaic virus strains involving reactive oxygen species and early transcriptomic changes
title Salicylic acid determines differential senescence produced by two turnip mosaic virus strains involving reactive oxygen species and early transcriptomic changes
spellingShingle Salicylic acid determines differential senescence produced by two turnip mosaic virus strains involving reactive oxygen species and early transcriptomic changes
Manacorda, Carlos Augusto
Acido Salicílico
Envejecimiento
Nabo
Brassica Juncea
Virus de las Plantas
Plant Viruses
Turnips
Aging
Salicylic Acids
Virus del Mosaico de Nabo
title_short Salicylic acid determines differential senescence produced by two turnip mosaic virus strains involving reactive oxygen species and early transcriptomic changes
title_full Salicylic acid determines differential senescence produced by two turnip mosaic virus strains involving reactive oxygen species and early transcriptomic changes
title_fullStr Salicylic acid determines differential senescence produced by two turnip mosaic virus strains involving reactive oxygen species and early transcriptomic changes
title_full_unstemmed Salicylic acid determines differential senescence produced by two turnip mosaic virus strains involving reactive oxygen species and early transcriptomic changes
title_sort Salicylic acid determines differential senescence produced by two turnip mosaic virus strains involving reactive oxygen species and early transcriptomic changes
dc.creator.none.fl_str_mv Manacorda, Carlos Augusto
Mansilla, Carmen
Debat, Humberto Julio
Zavallo, Diego
Sánchez, Flora
Ponz, Fernando
Asurmendi, Sebastian
author Manacorda, Carlos Augusto
author_facet Manacorda, Carlos Augusto
Mansilla, Carmen
Debat, Humberto Julio
Zavallo, Diego
Sánchez, Flora
Ponz, Fernando
Asurmendi, Sebastian
author_role author
author2 Mansilla, Carmen
Debat, Humberto Julio
Zavallo, Diego
Sánchez, Flora
Ponz, Fernando
Asurmendi, Sebastian
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Acido Salicílico
Envejecimiento
Nabo
Brassica Juncea
Virus de las Plantas
Plant Viruses
Turnips
Aging
Salicylic Acids
Virus del Mosaico de Nabo
topic Acido Salicílico
Envejecimiento
Nabo
Brassica Juncea
Virus de las Plantas
Plant Viruses
Turnips
Aging
Salicylic Acids
Virus del Mosaico de Nabo
dc.description.none.fl_txt_mv Losses produced by virus diseases depend mostly on symptom severity. Turnip mosaic virus (TuMV) is one of the most damaging and widespread potyvirus infecting members of the family Brassicaceae, including Arabidopsis thaliana. We used JPN1 and UK1 TuMV strains to characterize viral infections regarding symptom development, senescence progression, antioxidant response, reactive oxygen species (ROS) accumulation, and transcriptional profiling. Both isolates, despite accumulating similar viral titers, induced different symptomatology and strong differences in oxidative status. Early differences in several senescenceassociated genes linked to the ORE1 and ORS1 regulatory networks as well as persistent divergence in key ROS production and scavenging systems of the plant were detected. However, at a later stage, both strains induced nutrient competition, indicating that senescence rates are influenced by different mechanisms upon viral infections. Analyses of ORE1 and ORS1 levels in infected Brassica juncea plants showed a similar pattern, suggesting a conserved differential response to both strains in Brassicaceae spp. Transcriptional analysis of the ORE1 and ORS1 regulons showed similarities between salicylic acid (SA) response and the early induction triggered by UK1, the most severe strain. By means of SA-defective NahG transgenic plants, we found that differential senescence progression and ROS accumulation between strains rely on an intact SA pathway.
Fil: Manacorda, Carlos Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Mansilla, Carmen. Universidad Politécnica de Madrid. Centro de Biotecnología y Genómica de Plantas; España
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sánchez, Flora. Universidad Politécnica de Madrid. Centro de Biotecnología y Genómica de Plantas; España
Fil: Ponz, Fernando. Universidad Politécnica de Madrid. Centro de Biotecnología y Genómica de Plantas; España
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Losses produced by virus diseases depend mostly on symptom severity. Turnip mosaic virus (TuMV) is one of the most damaging and widespread potyvirus infecting members of the family Brassicaceae, including Arabidopsis thaliana. We used JPN1 and UK1 TuMV strains to characterize viral infections regarding symptom development, senescence progression, antioxidant response, reactive oxygen species (ROS) accumulation, and transcriptional profiling. Both isolates, despite accumulating similar viral titers, induced different symptomatology and strong differences in oxidative status. Early differences in several senescenceassociated genes linked to the ORE1 and ORS1 regulatory networks as well as persistent divergence in key ROS production and scavenging systems of the plant were detected. However, at a later stage, both strains induced nutrient competition, indicating that senescence rates are influenced by different mechanisms upon viral infections. Analyses of ORE1 and ORS1 levels in infected Brassica juncea plants showed a similar pattern, suggesting a conserved differential response to both strains in Brassicaceae spp. Transcriptional analysis of the ORE1 and ORS1 regulons showed similarities between salicylic acid (SA) response and the early induction triggered by UK1, the most severe strain. By means of SA-defective NahG transgenic plants, we found that differential senescence progression and ROS accumulation between strains rely on an intact SA pathway.
publishDate 2013
dc.date.none.fl_str_mv 2013-12
2018-04-05T18:11:25Z
2018-04-05T18:11:25Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/2184
https://apsjournals.apsnet.org/doi/pdf/10.1094/MPMI-07-13-0190-R
0894-0282
1943-7706 (online version)
http://dx.doi.org/10.1094/MPMI-07-13-0190-R
url http://hdl.handle.net/20.500.12123/2184
https://apsjournals.apsnet.org/doi/pdf/10.1094/MPMI-07-13-0190-R
http://dx.doi.org/10.1094/MPMI-07-13-0190-R
identifier_str_mv 0894-0282
1943-7706 (online version)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Molecular plant-microbe interactions 26 (12) : 1486-1498. (2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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