TuMV infection alters the regulation of miR168/AGO1 and miR403/AGO2 systems in Arabidopsis

Autores
Manacorda, Carlos Augusto; Tasselli, Sabrina; Marano, María Rosa; Asurmendi, Sebastian
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plant argonaute (AGO) proteins—chiefly AGO1 and 2—restrict viral infections. AGO1/2 also participate in developmental processes and are tightly regulated by microRNAs. Researchers have conducted extensive studies on the regulatory loop involving miR168/AGO1 in viral infections, though comparatively less attention has been given to the miR403/AGO2 system. Here, we simultaneously studied both regulatory systems in Arabidopsis plants infected with turnip mosaic virus (TuMV). TuMV simultaneously altered both miR168 and miR403 precursors as well as their mature forms at medium to late stages of infection. While TuMV decreased miRNA precursor molecules, it induced the overaccumulation of mature miRNA forms, without evidence of concomitant transcriptional alteration. The AGO1 protein remained at basal levels, whereas the AGO2 protein overaccumulated. The application of exogenous salicylic acid (SA) in healthy plants resulted in elevated AGO2 mRNA levels. Conversely, this hormone did not induce any significant changes in either AGO1 mRNA levels or those of miRs 168 and 403. This response is coherent with previous results, which showed enhanced levels of SA under TuMV infection and the partially differential sensitivity that AGO proteins have against this defense hormone. Our results also highlight the key role of AGO2 in leaves as an antiviral molecule and demonstrate the different responsiveness of the AGO1/miR168 and AGO2/miR403 systems regarding TuMV infection and SA response. Taken together, the results presented here are in line with previous reports studying abiotic and biotic impacts on microRNA biogenesis and AGO-dependent antiviral defense and further expand the knowledge of the miR403/AGO2 regulatory system.
Instituto de Biotecnología
Fil: Manacorda, Carlos Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Manacorda, Carlos Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tasselli, Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tasselli, Sabrina. Universidad Nacional de Rosario (UNR). Facultad de Ciencias Bioquímicas y Farmaceúticas. Instituto de Biología Molecular y Celular de Rosario (IBR); Argentina
Fil: Marano, María Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marano, María Rosa. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR); Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Plant Molecular Biology Reporter : 1-12 (Published: 29 April 2024)
Materia
Arabidopsis thaliana
Argonautidae
Viroses
MicroRNA
Infection
Virosis
MicroARN
Infección
Turnip Mosaic Virus
Virus del Mosaico del Nabo
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/18265
Acceder
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oai_identifier_str oai:localhost:20.500.12123/18265
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling TuMV infection alters the regulation of miR168/AGO1 and miR403/AGO2 systems in ArabidopsisManacorda, Carlos AugustoTasselli, SabrinaMarano, María RosaAsurmendi, SebastianArabidopsis thalianaArgonautidaeVirosesMicroRNAInfectionVirosisMicroARNInfecciónTurnip Mosaic VirusVirus del Mosaico del NaboPlant argonaute (AGO) proteins—chiefly AGO1 and 2—restrict viral infections. AGO1/2 also participate in developmental processes and are tightly regulated by microRNAs. Researchers have conducted extensive studies on the regulatory loop involving miR168/AGO1 in viral infections, though comparatively less attention has been given to the miR403/AGO2 system. Here, we simultaneously studied both regulatory systems in Arabidopsis plants infected with turnip mosaic virus (TuMV). TuMV simultaneously altered both miR168 and miR403 precursors as well as their mature forms at medium to late stages of infection. While TuMV decreased miRNA precursor molecules, it induced the overaccumulation of mature miRNA forms, without evidence of concomitant transcriptional alteration. The AGO1 protein remained at basal levels, whereas the AGO2 protein overaccumulated. The application of exogenous salicylic acid (SA) in healthy plants resulted in elevated AGO2 mRNA levels. Conversely, this hormone did not induce any significant changes in either AGO1 mRNA levels or those of miRs 168 and 403. This response is coherent with previous results, which showed enhanced levels of SA under TuMV infection and the partially differential sensitivity that AGO proteins have against this defense hormone. Our results also highlight the key role of AGO2 in leaves as an antiviral molecule and demonstrate the different responsiveness of the AGO1/miR168 and AGO2/miR403 systems regarding TuMV infection and SA response. Taken together, the results presented here are in line with previous reports studying abiotic and biotic impacts on microRNA biogenesis and AGO-dependent antiviral defense and further expand the knowledge of the miR403/AGO2 regulatory system.Instituto de BiotecnologíaFil: Manacorda, Carlos Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Manacorda, Carlos Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tasselli, Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tasselli, Sabrina. Universidad Nacional de Rosario (UNR). Facultad de Ciencias Bioquímicas y Farmaceúticas. Instituto de Biología Molecular y Celular de Rosario (IBR); ArgentinaFil: Marano, María Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Marano, María Rosa. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR); ArgentinaFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer2024-06-26T10:09:33Z2024-06-26T10:09:33Z2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/18265https://link.springer.com/article/10.1007/s11105-024-01461-61556-469Xhttps://doi.org/10.1007/s11105-024-01461-6Plant Molecular Biology Reporter : 1-12 (Published: 29 April 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L06-I116, Implementación de tecnologías y nuevas estrategias preventivas y terapéuticas para el desarrollo sustentable y eficiente de la producción animal en el marco de Una Saludinfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:36Zoai:localhost:20.500.12123/18265instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:37.181INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv TuMV infection alters the regulation of miR168/AGO1 and miR403/AGO2 systems in Arabidopsis
title TuMV infection alters the regulation of miR168/AGO1 and miR403/AGO2 systems in Arabidopsis
spellingShingle TuMV infection alters the regulation of miR168/AGO1 and miR403/AGO2 systems in Arabidopsis
Manacorda, Carlos Augusto
Arabidopsis thaliana
Argonautidae
Viroses
MicroRNA
Infection
Virosis
MicroARN
Infección
Turnip Mosaic Virus
Virus del Mosaico del Nabo
title_short TuMV infection alters the regulation of miR168/AGO1 and miR403/AGO2 systems in Arabidopsis
title_full TuMV infection alters the regulation of miR168/AGO1 and miR403/AGO2 systems in Arabidopsis
title_fullStr TuMV infection alters the regulation of miR168/AGO1 and miR403/AGO2 systems in Arabidopsis
title_full_unstemmed TuMV infection alters the regulation of miR168/AGO1 and miR403/AGO2 systems in Arabidopsis
title_sort TuMV infection alters the regulation of miR168/AGO1 and miR403/AGO2 systems in Arabidopsis
dc.creator.none.fl_str_mv Manacorda, Carlos Augusto
Tasselli, Sabrina
Marano, María Rosa
Asurmendi, Sebastian
author Manacorda, Carlos Augusto
author_facet Manacorda, Carlos Augusto
Tasselli, Sabrina
Marano, María Rosa
Asurmendi, Sebastian
author_role author
author2 Tasselli, Sabrina
Marano, María Rosa
Asurmendi, Sebastian
author2_role author
author
author
dc.subject.none.fl_str_mv Arabidopsis thaliana
Argonautidae
Viroses
MicroRNA
Infection
Virosis
MicroARN
Infección
Turnip Mosaic Virus
Virus del Mosaico del Nabo
topic Arabidopsis thaliana
Argonautidae
Viroses
MicroRNA
Infection
Virosis
MicroARN
Infección
Turnip Mosaic Virus
Virus del Mosaico del Nabo
dc.description.none.fl_txt_mv Plant argonaute (AGO) proteins—chiefly AGO1 and 2—restrict viral infections. AGO1/2 also participate in developmental processes and are tightly regulated by microRNAs. Researchers have conducted extensive studies on the regulatory loop involving miR168/AGO1 in viral infections, though comparatively less attention has been given to the miR403/AGO2 system. Here, we simultaneously studied both regulatory systems in Arabidopsis plants infected with turnip mosaic virus (TuMV). TuMV simultaneously altered both miR168 and miR403 precursors as well as their mature forms at medium to late stages of infection. While TuMV decreased miRNA precursor molecules, it induced the overaccumulation of mature miRNA forms, without evidence of concomitant transcriptional alteration. The AGO1 protein remained at basal levels, whereas the AGO2 protein overaccumulated. The application of exogenous salicylic acid (SA) in healthy plants resulted in elevated AGO2 mRNA levels. Conversely, this hormone did not induce any significant changes in either AGO1 mRNA levels or those of miRs 168 and 403. This response is coherent with previous results, which showed enhanced levels of SA under TuMV infection and the partially differential sensitivity that AGO proteins have against this defense hormone. Our results also highlight the key role of AGO2 in leaves as an antiviral molecule and demonstrate the different responsiveness of the AGO1/miR168 and AGO2/miR403 systems regarding TuMV infection and SA response. Taken together, the results presented here are in line with previous reports studying abiotic and biotic impacts on microRNA biogenesis and AGO-dependent antiviral defense and further expand the knowledge of the miR403/AGO2 regulatory system.
Instituto de Biotecnología
Fil: Manacorda, Carlos Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Manacorda, Carlos Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tasselli, Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tasselli, Sabrina. Universidad Nacional de Rosario (UNR). Facultad de Ciencias Bioquímicas y Farmaceúticas. Instituto de Biología Molecular y Celular de Rosario (IBR); Argentina
Fil: Marano, María Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marano, María Rosa. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR); Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Plant argonaute (AGO) proteins—chiefly AGO1 and 2—restrict viral infections. AGO1/2 also participate in developmental processes and are tightly regulated by microRNAs. Researchers have conducted extensive studies on the regulatory loop involving miR168/AGO1 in viral infections, though comparatively less attention has been given to the miR403/AGO2 system. Here, we simultaneously studied both regulatory systems in Arabidopsis plants infected with turnip mosaic virus (TuMV). TuMV simultaneously altered both miR168 and miR403 precursors as well as their mature forms at medium to late stages of infection. While TuMV decreased miRNA precursor molecules, it induced the overaccumulation of mature miRNA forms, without evidence of concomitant transcriptional alteration. The AGO1 protein remained at basal levels, whereas the AGO2 protein overaccumulated. The application of exogenous salicylic acid (SA) in healthy plants resulted in elevated AGO2 mRNA levels. Conversely, this hormone did not induce any significant changes in either AGO1 mRNA levels or those of miRs 168 and 403. This response is coherent with previous results, which showed enhanced levels of SA under TuMV infection and the partially differential sensitivity that AGO proteins have against this defense hormone. Our results also highlight the key role of AGO2 in leaves as an antiviral molecule and demonstrate the different responsiveness of the AGO1/miR168 and AGO2/miR403 systems regarding TuMV infection and SA response. Taken together, the results presented here are in line with previous reports studying abiotic and biotic impacts on microRNA biogenesis and AGO-dependent antiviral defense and further expand the knowledge of the miR403/AGO2 regulatory system.
publishDate 2024
dc.date.none.fl_str_mv 2024-06-26T10:09:33Z
2024-06-26T10:09:33Z
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/18265
https://link.springer.com/article/10.1007/s11105-024-01461-6
1556-469X
https://doi.org/10.1007/s11105-024-01461-6
url http://hdl.handle.net/20.500.12123/18265
https://link.springer.com/article/10.1007/s11105-024-01461-6
https://doi.org/10.1007/s11105-024-01461-6
identifier_str_mv 1556-469X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L06-I116, Implementación de tecnologías y nuevas estrategias preventivas y terapéuticas para el desarrollo sustentable y eficiente de la producción animal en el marco de Una Salud
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Plant Molecular Biology Reporter : 1-12 (Published: 29 April 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
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