TuMV triggers stomatal closure but reduces drought tolerance in Arabidopsis

Autores
Manacorda, Carlos Augusto; Gudesblat, Gustavo; Sutka, Moira; Alemano, Sergio Gabriel; Peluso, Franco; Oricchio, Patricio; Baroli, Irene; Asurmendi, Sebastian
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Compatible plant viral infections are a common cause of agricultural losses worldwide. Characterization of the physiological responses controlling plant water management under combined stresses is of great interest in the current climate change scenario. We studied the outcome of TuMV infection on stomatal closure and water balance, hormonal balance and drought tolerance in Arabidopsis. TuMV infection reduced stomatal aperture concomitantly with diminished gas exchange rate, daily water consumption and rosette initial dehydration rate. Infected plants overaccumulated salicylic acid and abscisic acid and showed altered expression levels of key ABA homeostasis genes including biosynthesis and catabolism. Also the expression of ABA signalling gene ABI2 was induced and ABCG40 (which imports ABA into guard cells) was highly induced upon infection. Hypermorfic abi2-1 mutant plants, but no other ABA or SA biosynthetic, signalling or degradation mutants tested abolished both stomatal closure and low stomatal conductance phenotypes caused by TuMV. Notwithstanding lower relative water loss during infection, plants simultaneously subjected to drought and viral stresses showed higher mortality rates than mock-inoculated drought stressed controls, alongside downregulation of drought-responsive gene RD29A. Our findings indicate that despite stomatal closure triggered by TuMV, additional phenomena diminish drought tolerance upon infection.
Instituto de Biotecnología
Fil: Manacorda, Carlos Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Manacorda, Carlos Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gudesblat, Gustavo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biociencias, Biotecnología y Biología Translacional. Departamento de Fisiología, Biología Molecular y Celular “Profesor Héctor Maldonado”; Argentina
Fil: Sutka, Moira. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad, Biología Experimental y Aplicada. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Sutka, Moira. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alemano, Sergio. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Laboratorio de Fisiología Vegetal; Argentina
Fil: Alemano, Sergio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Peluso, Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Clima y Agua; Argentina
Fil: Oricchio, Patricio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Clima y Agua; Argentina
Fil: Baroli, Irene. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad, Biología Experimental y Aplicada. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Baroli, Irene. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Plant, Cell and Environment 44 (5) : 1399-1416 (May 2021)
Materia
ABA
Arabidopsis
Salicylic Acids
Stomatal Conductance
Drought Tolerance
Ácido Salicílico
Conductancia Estomática
Tolerancia a la Sequia
Turnip Mosaic Virus
Virus del Mosaico del Nabo
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/23063
Acceder
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oai_identifier_str oai:localhost:20.500.12123/23063
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling TuMV triggers stomatal closure but reduces drought tolerance in ArabidopsisManacorda, Carlos AugustoGudesblat, GustavoSutka, MoiraAlemano, Sergio GabrielPeluso, FrancoOricchio, PatricioBaroli, IreneAsurmendi, SebastianABAArabidopsisSalicylic AcidsStomatal ConductanceDrought ToleranceÁcido SalicílicoConductancia EstomáticaTolerancia a la SequiaTurnip Mosaic VirusVirus del Mosaico del NaboCompatible plant viral infections are a common cause of agricultural losses worldwide. Characterization of the physiological responses controlling plant water management under combined stresses is of great interest in the current climate change scenario. We studied the outcome of TuMV infection on stomatal closure and water balance, hormonal balance and drought tolerance in Arabidopsis. TuMV infection reduced stomatal aperture concomitantly with diminished gas exchange rate, daily water consumption and rosette initial dehydration rate. Infected plants overaccumulated salicylic acid and abscisic acid and showed altered expression levels of key ABA homeostasis genes including biosynthesis and catabolism. Also the expression of ABA signalling gene ABI2 was induced and ABCG40 (which imports ABA into guard cells) was highly induced upon infection. Hypermorfic abi2-1 mutant plants, but no other ABA or SA biosynthetic, signalling or degradation mutants tested abolished both stomatal closure and low stomatal conductance phenotypes caused by TuMV. Notwithstanding lower relative water loss during infection, plants simultaneously subjected to drought and viral stresses showed higher mortality rates than mock-inoculated drought stressed controls, alongside downregulation of drought-responsive gene RD29A. Our findings indicate that despite stomatal closure triggered by TuMV, additional phenomena diminish drought tolerance upon infection.Instituto de BiotecnologíaFil: Manacorda, Carlos Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Manacorda, Carlos Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gudesblat, Gustavo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biociencias, Biotecnología y Biología Translacional. Departamento de Fisiología, Biología Molecular y Celular “Profesor Héctor Maldonado”; ArgentinaFil: Sutka, Moira. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad, Biología Experimental y Aplicada. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Sutka, Moira. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alemano, Sergio. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Laboratorio de Fisiología Vegetal; ArgentinaFil: Alemano, Sergio. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Peluso, Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Clima y Agua; ArgentinaFil: Oricchio, Patricio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Clima y Agua; ArgentinaFil: Baroli, Irene. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad, Biología Experimental y Aplicada. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Baroli, Irene. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley2025-07-17T13:38:58Z2025-07-17T13:38:58Z2021-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/23063https://onlinelibrary.wiley.com/doi/10.1111/pce.140241365-3040https://doi.org/10.1111/pce.14024Plant, Cell and Environment 44 (5) : 1399-1416 (May 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E6-I116-001, Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrialinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:51:11Zoai:localhost:20.500.12123/23063instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:51:11.722INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv TuMV triggers stomatal closure but reduces drought tolerance in Arabidopsis
title TuMV triggers stomatal closure but reduces drought tolerance in Arabidopsis
spellingShingle TuMV triggers stomatal closure but reduces drought tolerance in Arabidopsis
Manacorda, Carlos Augusto
ABA
Arabidopsis
Salicylic Acids
Stomatal Conductance
Drought Tolerance
Ácido Salicílico
Conductancia Estomática
Tolerancia a la Sequia
Turnip Mosaic Virus
Virus del Mosaico del Nabo
title_short TuMV triggers stomatal closure but reduces drought tolerance in Arabidopsis
title_full TuMV triggers stomatal closure but reduces drought tolerance in Arabidopsis
title_fullStr TuMV triggers stomatal closure but reduces drought tolerance in Arabidopsis
title_full_unstemmed TuMV triggers stomatal closure but reduces drought tolerance in Arabidopsis
title_sort TuMV triggers stomatal closure but reduces drought tolerance in Arabidopsis
dc.creator.none.fl_str_mv Manacorda, Carlos Augusto
Gudesblat, Gustavo
Sutka, Moira
Alemano, Sergio Gabriel
Peluso, Franco
Oricchio, Patricio
Baroli, Irene
Asurmendi, Sebastian
author Manacorda, Carlos Augusto
author_facet Manacorda, Carlos Augusto
Gudesblat, Gustavo
Sutka, Moira
Alemano, Sergio Gabriel
Peluso, Franco
Oricchio, Patricio
Baroli, Irene
Asurmendi, Sebastian
author_role author
author2 Gudesblat, Gustavo
Sutka, Moira
Alemano, Sergio Gabriel
Peluso, Franco
Oricchio, Patricio
Baroli, Irene
Asurmendi, Sebastian
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ABA
Arabidopsis
Salicylic Acids
Stomatal Conductance
Drought Tolerance
Ácido Salicílico
Conductancia Estomática
Tolerancia a la Sequia
Turnip Mosaic Virus
Virus del Mosaico del Nabo
topic ABA
Arabidopsis
Salicylic Acids
Stomatal Conductance
Drought Tolerance
Ácido Salicílico
Conductancia Estomática
Tolerancia a la Sequia
Turnip Mosaic Virus
Virus del Mosaico del Nabo
dc.description.none.fl_txt_mv Compatible plant viral infections are a common cause of agricultural losses worldwide. Characterization of the physiological responses controlling plant water management under combined stresses is of great interest in the current climate change scenario. We studied the outcome of TuMV infection on stomatal closure and water balance, hormonal balance and drought tolerance in Arabidopsis. TuMV infection reduced stomatal aperture concomitantly with diminished gas exchange rate, daily water consumption and rosette initial dehydration rate. Infected plants overaccumulated salicylic acid and abscisic acid and showed altered expression levels of key ABA homeostasis genes including biosynthesis and catabolism. Also the expression of ABA signalling gene ABI2 was induced and ABCG40 (which imports ABA into guard cells) was highly induced upon infection. Hypermorfic abi2-1 mutant plants, but no other ABA or SA biosynthetic, signalling or degradation mutants tested abolished both stomatal closure and low stomatal conductance phenotypes caused by TuMV. Notwithstanding lower relative water loss during infection, plants simultaneously subjected to drought and viral stresses showed higher mortality rates than mock-inoculated drought stressed controls, alongside downregulation of drought-responsive gene RD29A. Our findings indicate that despite stomatal closure triggered by TuMV, additional phenomena diminish drought tolerance upon infection.
Instituto de Biotecnología
Fil: Manacorda, Carlos Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Manacorda, Carlos Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gudesblat, Gustavo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biociencias, Biotecnología y Biología Translacional. Departamento de Fisiología, Biología Molecular y Celular “Profesor Héctor Maldonado”; Argentina
Fil: Sutka, Moira. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad, Biología Experimental y Aplicada. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Sutka, Moira. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alemano, Sergio. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Laboratorio de Fisiología Vegetal; Argentina
Fil: Alemano, Sergio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Peluso, Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Clima y Agua; Argentina
Fil: Oricchio, Patricio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Clima y Agua; Argentina
Fil: Baroli, Irene. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad, Biología Experimental y Aplicada. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Baroli, Irene. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Compatible plant viral infections are a common cause of agricultural losses worldwide. Characterization of the physiological responses controlling plant water management under combined stresses is of great interest in the current climate change scenario. We studied the outcome of TuMV infection on stomatal closure and water balance, hormonal balance and drought tolerance in Arabidopsis. TuMV infection reduced stomatal aperture concomitantly with diminished gas exchange rate, daily water consumption and rosette initial dehydration rate. Infected plants overaccumulated salicylic acid and abscisic acid and showed altered expression levels of key ABA homeostasis genes including biosynthesis and catabolism. Also the expression of ABA signalling gene ABI2 was induced and ABCG40 (which imports ABA into guard cells) was highly induced upon infection. Hypermorfic abi2-1 mutant plants, but no other ABA or SA biosynthetic, signalling or degradation mutants tested abolished both stomatal closure and low stomatal conductance phenotypes caused by TuMV. Notwithstanding lower relative water loss during infection, plants simultaneously subjected to drought and viral stresses showed higher mortality rates than mock-inoculated drought stressed controls, alongside downregulation of drought-responsive gene RD29A. Our findings indicate that despite stomatal closure triggered by TuMV, additional phenomena diminish drought tolerance upon infection.
publishDate 2021
dc.date.none.fl_str_mv 2021-05
2025-07-17T13:38:58Z
2025-07-17T13:38:58Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/23063
https://onlinelibrary.wiley.com/doi/10.1111/pce.14024
1365-3040
https://doi.org/10.1111/pce.14024
url http://hdl.handle.net/20.500.12123/23063
https://onlinelibrary.wiley.com/doi/10.1111/pce.14024
https://doi.org/10.1111/pce.14024
identifier_str_mv 1365-3040
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E6-I116-001, Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrial
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Plant, Cell and Environment 44 (5) : 1399-1416 (May 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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