Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle

Autores
Soria, Ivana; Quattrocchi, Valeria; Langellotti, Cecilia Ana; Gammella, Mariela; Di Giacomo, Sebastián; Garcia de la Torre, Beatriz; Andreu, David; Montoya, Maria; Sobrino, Francisco; Blanco, Esther; Zamorano, Patricia Ines
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136–154)] linked to a T-cell epitope [3A(21–35)] of FMDV confers protection to type O FMDV challenge in pigs. Herein we show in cattle that novel dendrimeric peptides bearing a T-cell epitope [VP1(21–40] and two or four copies of a B-cell epitope [VP1(135–160)] from type O1 Campos FMDV (termed B2T and B4T, respectively) elicited FMDV specific immune responses to similar levels to a commercial vaccine. Animals were challenged with FMDV and 100% of vaccinated cattle with B2T or B4T were protected to podal generalization. Moreover, bovines immunized with B4T were completely protected (with no clinical signs) against FMDV challenge after three vaccine doses, which was associated with titers of viral neutralizing antibodies in serum higher than those of B2T group (p< 0.05) and levels of opsonic antibodies similar to those of animals immunized with one dose of FMDV commercial vaccine. Bovines vaccinated with both dendrimeric peptides presented high levels of IgG1 anti FMDV in sera and in mucosa. When IgA in nasal secretions was measured, 20% or 40% of the animals in B2T or B4T groups respectively, showed anti-FMDV IgA titers. In addition, B2T and B4T peptides evoked similar consistent T cell responses, being recognized in vitro by lymphocytes from most of the immunized cattle in the proliferation assay, and from all animals in the IFN-γ production assay. Taken together, these results support the potential of dendrimers B2T or B4T in cattle as a highly valuable, cost-effective FMDV candidate vaccine with DIVA potential.
Instituto de Virología
Fil: Soria, Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gammella, Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Di Giacomo, Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Garcia de la Torre, Beatriz. Universitat Pompeu-Fabra. Departament de Ciencies Experimentals i de la Salut; España
Fil: Andreu, David. Universitat Pompeu-Fabra. Departament de Ciencies Experimentals i de la Salut; España
Fil: Montoya, María. Pirbright Institute; Gran Bretaña
Fil: Sobrino, Francisco. Universidad Autónoma de Madrid. Centro de Biología Molecular “Severo Ochoa"; España
Fil: Blanco, Esther. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Centro de Investigación en Sanidad Animal; España
Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
PLoS ONE 12 (9) : e0185184 (2017)
Materia
Enfermedades de los Animales
Virus de los Animales
Ganado Bovino
Fiebre Aftosa
Péptidos
Animal Diseases
Animal Viruses
Cattle
Foot and Mouth Disease
Peptides
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2006
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oai_identifier_str oai:localhost:20.500.12123/2006
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repository_id_str l
network_name_str INTA Digital (INTA)
spelling Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattleSoria, IvanaQuattrocchi, ValeriaLangellotti, Cecilia AnaGammella, MarielaDi Giacomo, SebastiánGarcia de la Torre, BeatrizAndreu, DavidMontoya, MariaSobrino, FranciscoBlanco, EstherZamorano, Patricia InesEnfermedades de los AnimalesVirus de los AnimalesGanado BovinoFiebre AftosaPéptidosAnimal DiseasesAnimal VirusesCattleFoot and Mouth DiseasePeptidesFoot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136–154)] linked to a T-cell epitope [3A(21–35)] of FMDV confers protection to type O FMDV challenge in pigs. Herein we show in cattle that novel dendrimeric peptides bearing a T-cell epitope [VP1(21–40] and two or four copies of a B-cell epitope [VP1(135–160)] from type O1 Campos FMDV (termed B2T and B4T, respectively) elicited FMDV specific immune responses to similar levels to a commercial vaccine. Animals were challenged with FMDV and 100% of vaccinated cattle with B2T or B4T were protected to podal generalization. Moreover, bovines immunized with B4T were completely protected (with no clinical signs) against FMDV challenge after three vaccine doses, which was associated with titers of viral neutralizing antibodies in serum higher than those of B2T group (p< 0.05) and levels of opsonic antibodies similar to those of animals immunized with one dose of FMDV commercial vaccine. Bovines vaccinated with both dendrimeric peptides presented high levels of IgG1 anti FMDV in sera and in mucosa. When IgA in nasal secretions was measured, 20% or 40% of the animals in B2T or B4T groups respectively, showed anti-FMDV IgA titers. In addition, B2T and B4T peptides evoked similar consistent T cell responses, being recognized in vitro by lymphocytes from most of the immunized cattle in the proliferation assay, and from all animals in the IFN-γ production assay. Taken together, these results support the potential of dendrimers B2T or B4T in cattle as a highly valuable, cost-effective FMDV candidate vaccine with DIVA potential.Instituto de VirologíaFil: Soria, Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gammella, Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Di Giacomo, Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Garcia de la Torre, Beatriz. Universitat Pompeu-Fabra. Departament de Ciencies Experimentals i de la Salut; EspañaFil: Andreu, David. Universitat Pompeu-Fabra. Departament de Ciencies Experimentals i de la Salut; EspañaFil: Montoya, María. Pirbright Institute; Gran BretañaFil: Sobrino, Francisco. Universidad Autónoma de Madrid. Centro de Biología Molecular “Severo Ochoa"; EspañaFil: Blanco, Esther. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Centro de Investigación en Sanidad Animal; EspañaFil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina2018-03-09T15:54:33Z2018-03-09T15:54:33Z2017-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/2006http://journals.plos.org/plosone/article?id=10.1371/journal.pone.01851841932-6203https://doi.org/10.1371/journal.pone.0185184PLoS ONE 12 (9) : e0185184 (2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:29:06Zoai:localhost:20.500.12123/2006instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:06.877INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
title Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
spellingShingle Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
Soria, Ivana
Enfermedades de los Animales
Virus de los Animales
Ganado Bovino
Fiebre Aftosa
Péptidos
Animal Diseases
Animal Viruses
Cattle
Foot and Mouth Disease
Peptides
title_short Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
title_full Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
title_fullStr Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
title_full_unstemmed Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
title_sort Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
dc.creator.none.fl_str_mv Soria, Ivana
Quattrocchi, Valeria
Langellotti, Cecilia Ana
Gammella, Mariela
Di Giacomo, Sebastián
Garcia de la Torre, Beatriz
Andreu, David
Montoya, Maria
Sobrino, Francisco
Blanco, Esther
Zamorano, Patricia Ines
author Soria, Ivana
author_facet Soria, Ivana
Quattrocchi, Valeria
Langellotti, Cecilia Ana
Gammella, Mariela
Di Giacomo, Sebastián
Garcia de la Torre, Beatriz
Andreu, David
Montoya, Maria
Sobrino, Francisco
Blanco, Esther
Zamorano, Patricia Ines
author_role author
author2 Quattrocchi, Valeria
Langellotti, Cecilia Ana
Gammella, Mariela
Di Giacomo, Sebastián
Garcia de la Torre, Beatriz
Andreu, David
Montoya, Maria
Sobrino, Francisco
Blanco, Esther
Zamorano, Patricia Ines
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Enfermedades de los Animales
Virus de los Animales
Ganado Bovino
Fiebre Aftosa
Péptidos
Animal Diseases
Animal Viruses
Cattle
Foot and Mouth Disease
Peptides
topic Enfermedades de los Animales
Virus de los Animales
Ganado Bovino
Fiebre Aftosa
Péptidos
Animal Diseases
Animal Viruses
Cattle
Foot and Mouth Disease
Peptides
dc.description.none.fl_txt_mv Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136–154)] linked to a T-cell epitope [3A(21–35)] of FMDV confers protection to type O FMDV challenge in pigs. Herein we show in cattle that novel dendrimeric peptides bearing a T-cell epitope [VP1(21–40] and two or four copies of a B-cell epitope [VP1(135–160)] from type O1 Campos FMDV (termed B2T and B4T, respectively) elicited FMDV specific immune responses to similar levels to a commercial vaccine. Animals were challenged with FMDV and 100% of vaccinated cattle with B2T or B4T were protected to podal generalization. Moreover, bovines immunized with B4T were completely protected (with no clinical signs) against FMDV challenge after three vaccine doses, which was associated with titers of viral neutralizing antibodies in serum higher than those of B2T group (p< 0.05) and levels of opsonic antibodies similar to those of animals immunized with one dose of FMDV commercial vaccine. Bovines vaccinated with both dendrimeric peptides presented high levels of IgG1 anti FMDV in sera and in mucosa. When IgA in nasal secretions was measured, 20% or 40% of the animals in B2T or B4T groups respectively, showed anti-FMDV IgA titers. In addition, B2T and B4T peptides evoked similar consistent T cell responses, being recognized in vitro by lymphocytes from most of the immunized cattle in the proliferation assay, and from all animals in the IFN-γ production assay. Taken together, these results support the potential of dendrimers B2T or B4T in cattle as a highly valuable, cost-effective FMDV candidate vaccine with DIVA potential.
Instituto de Virología
Fil: Soria, Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gammella, Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Di Giacomo, Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Garcia de la Torre, Beatriz. Universitat Pompeu-Fabra. Departament de Ciencies Experimentals i de la Salut; España
Fil: Andreu, David. Universitat Pompeu-Fabra. Departament de Ciencies Experimentals i de la Salut; España
Fil: Montoya, María. Pirbright Institute; Gran Bretaña
Fil: Sobrino, Francisco. Universidad Autónoma de Madrid. Centro de Biología Molecular “Severo Ochoa"; España
Fil: Blanco, Esther. Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria. Centro de Investigación en Sanidad Animal; España
Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136–154)] linked to a T-cell epitope [3A(21–35)] of FMDV confers protection to type O FMDV challenge in pigs. Herein we show in cattle that novel dendrimeric peptides bearing a T-cell epitope [VP1(21–40] and two or four copies of a B-cell epitope [VP1(135–160)] from type O1 Campos FMDV (termed B2T and B4T, respectively) elicited FMDV specific immune responses to similar levels to a commercial vaccine. Animals were challenged with FMDV and 100% of vaccinated cattle with B2T or B4T were protected to podal generalization. Moreover, bovines immunized with B4T were completely protected (with no clinical signs) against FMDV challenge after three vaccine doses, which was associated with titers of viral neutralizing antibodies in serum higher than those of B2T group (p< 0.05) and levels of opsonic antibodies similar to those of animals immunized with one dose of FMDV commercial vaccine. Bovines vaccinated with both dendrimeric peptides presented high levels of IgG1 anti FMDV in sera and in mucosa. When IgA in nasal secretions was measured, 20% or 40% of the animals in B2T or B4T groups respectively, showed anti-FMDV IgA titers. In addition, B2T and B4T peptides evoked similar consistent T cell responses, being recognized in vitro by lymphocytes from most of the immunized cattle in the proliferation assay, and from all animals in the IFN-γ production assay. Taken together, these results support the potential of dendrimers B2T or B4T in cattle as a highly valuable, cost-effective FMDV candidate vaccine with DIVA potential.
publishDate 2017
dc.date.none.fl_str_mv 2017-09
2018-03-09T15:54:33Z
2018-03-09T15:54:33Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/2006
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0185184
1932-6203
https://doi.org/10.1371/journal.pone.0185184
url http://hdl.handle.net/20.500.12123/2006
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0185184
https://doi.org/10.1371/journal.pone.0185184
identifier_str_mv 1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv PLoS ONE 12 (9) : e0185184 (2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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