VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display

Autores
Peralta, Andrea Veronica; Maroniche, Guillermo Andrés; Alfonso, Victoria; Molinari, Maria Paula; Taboga, Oscar Alberto
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The Foot-and-mouth disease virus (FMDV) causes important economical losses in livestock farming. In order to develop a novel subunit vaccine against FMDV, we constructed recombinant baculoviruses that display the protein VP1 of FMDV on their surface, with either polar (fused to gp64) or nonpolar (fused to anchor membrane from VSV-G protein) distribution. Insect cells infected with the different recombinant baculoviruses expressed VP1 fusion protein to high levels. However, the recombinant VP1 protein was not carried by budded virions. Subcellular localization of VP1 revealed that the trafficking of the fusion protein to the cell plasma membrane was impaired. Our results suggest that VP1 contains cryptic domains that interfere with protein secretion and subsequent incorporation into budded baculoviruses.
Instituto de Biotecnología
Fil: Peralta, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Maroniche, Guillermo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alfonso, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Molinari, Maria Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Virus Research 175 (1) : 87-90 (July 2013)
Materia
Enfermedades de los Animales
Virus Fiebre Aftosa
Fiebre Aftosa
Ganado
Baculovirus
Animal Diseases
Aphthovirus
Foot and Mouth Disease
Livestock
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4355
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4355
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network_name_str INTA Digital (INTA)
spelling VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface displayPeralta, Andrea VeronicaMaroniche, Guillermo AndrésAlfonso, VictoriaMolinari, Maria PaulaTaboga, Oscar AlbertoEnfermedades de los AnimalesVirus Fiebre AftosaFiebre AftosaGanadoBaculovirusAnimal DiseasesAphthovirusFoot and Mouth DiseaseLivestockThe Foot-and-mouth disease virus (FMDV) causes important economical losses in livestock farming. In order to develop a novel subunit vaccine against FMDV, we constructed recombinant baculoviruses that display the protein VP1 of FMDV on their surface, with either polar (fused to gp64) or nonpolar (fused to anchor membrane from VSV-G protein) distribution. Insect cells infected with the different recombinant baculoviruses expressed VP1 fusion protein to high levels. However, the recombinant VP1 protein was not carried by budded virions. Subcellular localization of VP1 revealed that the trafficking of the fusion protein to the cell plasma membrane was impaired. Our results suggest that VP1 contains cryptic domains that interfere with protein secretion and subsequent incorporation into budded baculoviruses.Instituto de BiotecnologíaFil: Peralta, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Maroniche, Guillermo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alfonso, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Molinari, Maria Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2019-01-30T12:12:29Z2019-01-30T12:12:29Z2013-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0168170213001123http://hdl.handle.net/20.500.12123/43550168-1702https://doi.org/10.1016/j.virusres.2013.03.018Virus Research 175 (1) : 87-90 (July 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:33Zoai:localhost:20.500.12123/4355instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:34.218INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display
title VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display
spellingShingle VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display
Peralta, Andrea Veronica
Enfermedades de los Animales
Virus Fiebre Aftosa
Fiebre Aftosa
Ganado
Baculovirus
Animal Diseases
Aphthovirus
Foot and Mouth Disease
Livestock
title_short VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display
title_full VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display
title_fullStr VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display
title_full_unstemmed VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display
title_sort VP1 protein of Foot-and-mouth disease virus (FMDV) impairs baculovirus surface display
dc.creator.none.fl_str_mv Peralta, Andrea Veronica
Maroniche, Guillermo Andrés
Alfonso, Victoria
Molinari, Maria Paula
Taboga, Oscar Alberto
author Peralta, Andrea Veronica
author_facet Peralta, Andrea Veronica
Maroniche, Guillermo Andrés
Alfonso, Victoria
Molinari, Maria Paula
Taboga, Oscar Alberto
author_role author
author2 Maroniche, Guillermo Andrés
Alfonso, Victoria
Molinari, Maria Paula
Taboga, Oscar Alberto
author2_role author
author
author
author
dc.subject.none.fl_str_mv Enfermedades de los Animales
Virus Fiebre Aftosa
Fiebre Aftosa
Ganado
Baculovirus
Animal Diseases
Aphthovirus
Foot and Mouth Disease
Livestock
topic Enfermedades de los Animales
Virus Fiebre Aftosa
Fiebre Aftosa
Ganado
Baculovirus
Animal Diseases
Aphthovirus
Foot and Mouth Disease
Livestock
dc.description.none.fl_txt_mv The Foot-and-mouth disease virus (FMDV) causes important economical losses in livestock farming. In order to develop a novel subunit vaccine against FMDV, we constructed recombinant baculoviruses that display the protein VP1 of FMDV on their surface, with either polar (fused to gp64) or nonpolar (fused to anchor membrane from VSV-G protein) distribution. Insect cells infected with the different recombinant baculoviruses expressed VP1 fusion protein to high levels. However, the recombinant VP1 protein was not carried by budded virions. Subcellular localization of VP1 revealed that the trafficking of the fusion protein to the cell plasma membrane was impaired. Our results suggest that VP1 contains cryptic domains that interfere with protein secretion and subsequent incorporation into budded baculoviruses.
Instituto de Biotecnología
Fil: Peralta, Andrea Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Maroniche, Guillermo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alfonso, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Molinari, Maria Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The Foot-and-mouth disease virus (FMDV) causes important economical losses in livestock farming. In order to develop a novel subunit vaccine against FMDV, we constructed recombinant baculoviruses that display the protein VP1 of FMDV on their surface, with either polar (fused to gp64) or nonpolar (fused to anchor membrane from VSV-G protein) distribution. Insect cells infected with the different recombinant baculoviruses expressed VP1 fusion protein to high levels. However, the recombinant VP1 protein was not carried by budded virions. Subcellular localization of VP1 revealed that the trafficking of the fusion protein to the cell plasma membrane was impaired. Our results suggest that VP1 contains cryptic domains that interfere with protein secretion and subsequent incorporation into budded baculoviruses.
publishDate 2013
dc.date.none.fl_str_mv 2013-07
2019-01-30T12:12:29Z
2019-01-30T12:12:29Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0168170213001123
http://hdl.handle.net/20.500.12123/4355
0168-1702
https://doi.org/10.1016/j.virusres.2013.03.018
url https://www.sciencedirect.com/science/article/pii/S0168170213001123
http://hdl.handle.net/20.500.12123/4355
https://doi.org/10.1016/j.virusres.2013.03.018
identifier_str_mv 0168-1702
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Virus Research 175 (1) : 87-90 (July 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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