Evaluation of immune responses of stabilised SAT2 antigens of foot-and-mouth disease in cattle

Autores
Scott, Katherine A.; Rathogwa, N.M.; Capozzo, Alejandra; Maree, Francois F.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Foot-and-mouth disease (FMD) vaccines with improved stability and less reliant on a cold-chain are needed to improve the longevity of immune responses elicited in animals. This is especially so for serotypes O and SAT2 which are unstable in mildly acidic pH conditions or at elevated temperatures leading to dissociation of the capsid (146S particle) and loss of immunogenicity. Previously, stabilised SAT2 viruses were generated by reverse genetic approaches and assessed in vitro and in vivo with a guinea pig trial. Here we investigated the efficacy and comparative immunological responses of two thermostable and wild-type SAT2 vaccines over 5 months followed by challenge. We assessed humoral immune responses elicited in cattle in terms of total and neutralizing antibodies and IgG1/2 isotyping; and cell-mediated responses of IFN-γ as in vitro markers of protection. Whilst there were significant differences in total and neutralizing antibodies for the vSAT2-93H group compared to other vaccinated groups after the first vaccination, there were no significant differences after the second immunization. Following intra-dermolingual challenge all vaccinated groups were fully protected as determined by the absence of generalized lesions. These results provide proof that two vaccine doses, consisting of SAT2 antigen combined with ISA206B adjuvant, administered 4–6 weeks apart were able to protect animals up to 5 months pv. Additionally, vSAT2-93Y had significantly higher levels of IFN-γ after challenge and had a lower clinical score indicative of better protection compared to other vaccinated groups and the importance of cell mediated responses and antigen stability in protection.
Inst.de Virología
Fil: Scott, Katherine A. Agricultural Research Council. Onderstepoort Veterinary Institute. Transboundary Animal Diseases Programme; Sudáfrica. University of Pretoria. Faculty of Veterinary Science. Department of Veterinary Tropical Diseases; Sudáfrica
Fil: Rathogwa, N.M. Agricultural Research Council. Onderstepoort Veterinary Institute. Transboundary Animal Diseases Programme; Sudáfrica. University of Pretoria. Faculty of Agricultural and Natural Sciences. Department of Microbiology and Plant Pathology; Sudáfrica
Fil: Capozzo, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Maree, Francois F. Agricultural Research Council. Onderstepoort Veterinary Institute. Transboundary Animal Diseases Programme; Sudáfrica. University of Pretoria. Faculty of Agricultural and Natural Sciences. Department of Microbiology and Plant Pathology; Sudáfrica
Fuente
Vaccine 35 (40) : 5426-5433. (September 2017)
Materia
Ganado Bovino
Enfermedades de los Animales
Virus de los Animales
Fiebre Aftosa
Cattle
Animal Diseases
Animal Viruses
Foot and Mouth Disease
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2007
Acceder
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spelling Evaluation of immune responses of stabilised SAT2 antigens of foot-and-mouth disease in cattleScott, Katherine A.Rathogwa, N.M.Capozzo, AlejandraMaree, Francois F.Ganado BovinoEnfermedades de los AnimalesVirus de los AnimalesFiebre AftosaCattleAnimal DiseasesAnimal VirusesFoot and Mouth DiseaseFoot-and-mouth disease (FMD) vaccines with improved stability and less reliant on a cold-chain are needed to improve the longevity of immune responses elicited in animals. This is especially so for serotypes O and SAT2 which are unstable in mildly acidic pH conditions or at elevated temperatures leading to dissociation of the capsid (146S particle) and loss of immunogenicity. Previously, stabilised SAT2 viruses were generated by reverse genetic approaches and assessed in vitro and in vivo with a guinea pig trial. Here we investigated the efficacy and comparative immunological responses of two thermostable and wild-type SAT2 vaccines over 5 months followed by challenge. We assessed humoral immune responses elicited in cattle in terms of total and neutralizing antibodies and IgG1/2 isotyping; and cell-mediated responses of IFN-γ as in vitro markers of protection. Whilst there were significant differences in total and neutralizing antibodies for the vSAT2-93H group compared to other vaccinated groups after the first vaccination, there were no significant differences after the second immunization. Following intra-dermolingual challenge all vaccinated groups were fully protected as determined by the absence of generalized lesions. These results provide proof that two vaccine doses, consisting of SAT2 antigen combined with ISA206B adjuvant, administered 4–6 weeks apart were able to protect animals up to 5 months pv. Additionally, vSAT2-93Y had significantly higher levels of IFN-γ after challenge and had a lower clinical score indicative of better protection compared to other vaccinated groups and the importance of cell mediated responses and antigen stability in protection.Inst.de VirologíaFil: Scott, Katherine A. Agricultural Research Council. Onderstepoort Veterinary Institute. Transboundary Animal Diseases Programme; Sudáfrica. University of Pretoria. Faculty of Veterinary Science. Department of Veterinary Tropical Diseases; SudáfricaFil: Rathogwa, N.M. Agricultural Research Council. Onderstepoort Veterinary Institute. Transboundary Animal Diseases Programme; Sudáfrica. University of Pretoria. Faculty of Agricultural and Natural Sciences. Department of Microbiology and Plant Pathology; SudáfricaFil: Capozzo, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Maree, Francois F. Agricultural Research Council. Onderstepoort Veterinary Institute. Transboundary Animal Diseases Programme; Sudáfrica. University of Pretoria. Faculty of Agricultural and Natural Sciences. Department of Microbiology and Plant Pathology; Sudáfrica2018-03-09T16:33:26Z2018-03-09T16:33:26Z2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/2007https://www.sciencedirect.com/science/article/pii/S0264410X17301664?via%3Dihub0264-410Xhttps://doi.org/10.1016/j.vaccine.2017.02.003Vaccine 35 (40) : 5426-5433. (September 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-18T10:07:05Zoai:localhost:20.500.12123/2007instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-18 10:07:05.499INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Evaluation of immune responses of stabilised SAT2 antigens of foot-and-mouth disease in cattle
title Evaluation of immune responses of stabilised SAT2 antigens of foot-and-mouth disease in cattle
spellingShingle Evaluation of immune responses of stabilised SAT2 antigens of foot-and-mouth disease in cattle
Scott, Katherine A.
Ganado Bovino
Enfermedades de los Animales
Virus de los Animales
Fiebre Aftosa
Cattle
Animal Diseases
Animal Viruses
Foot and Mouth Disease
title_short Evaluation of immune responses of stabilised SAT2 antigens of foot-and-mouth disease in cattle
title_full Evaluation of immune responses of stabilised SAT2 antigens of foot-and-mouth disease in cattle
title_fullStr Evaluation of immune responses of stabilised SAT2 antigens of foot-and-mouth disease in cattle
title_full_unstemmed Evaluation of immune responses of stabilised SAT2 antigens of foot-and-mouth disease in cattle
title_sort Evaluation of immune responses of stabilised SAT2 antigens of foot-and-mouth disease in cattle
dc.creator.none.fl_str_mv Scott, Katherine A.
Rathogwa, N.M.
Capozzo, Alejandra
Maree, Francois F.
author Scott, Katherine A.
author_facet Scott, Katherine A.
Rathogwa, N.M.
Capozzo, Alejandra
Maree, Francois F.
author_role author
author2 Rathogwa, N.M.
Capozzo, Alejandra
Maree, Francois F.
author2_role author
author
author
dc.subject.none.fl_str_mv Ganado Bovino
Enfermedades de los Animales
Virus de los Animales
Fiebre Aftosa
Cattle
Animal Diseases
Animal Viruses
Foot and Mouth Disease
topic Ganado Bovino
Enfermedades de los Animales
Virus de los Animales
Fiebre Aftosa
Cattle
Animal Diseases
Animal Viruses
Foot and Mouth Disease
dc.description.none.fl_txt_mv Foot-and-mouth disease (FMD) vaccines with improved stability and less reliant on a cold-chain are needed to improve the longevity of immune responses elicited in animals. This is especially so for serotypes O and SAT2 which are unstable in mildly acidic pH conditions or at elevated temperatures leading to dissociation of the capsid (146S particle) and loss of immunogenicity. Previously, stabilised SAT2 viruses were generated by reverse genetic approaches and assessed in vitro and in vivo with a guinea pig trial. Here we investigated the efficacy and comparative immunological responses of two thermostable and wild-type SAT2 vaccines over 5 months followed by challenge. We assessed humoral immune responses elicited in cattle in terms of total and neutralizing antibodies and IgG1/2 isotyping; and cell-mediated responses of IFN-γ as in vitro markers of protection. Whilst there were significant differences in total and neutralizing antibodies for the vSAT2-93H group compared to other vaccinated groups after the first vaccination, there were no significant differences after the second immunization. Following intra-dermolingual challenge all vaccinated groups were fully protected as determined by the absence of generalized lesions. These results provide proof that two vaccine doses, consisting of SAT2 antigen combined with ISA206B adjuvant, administered 4–6 weeks apart were able to protect animals up to 5 months pv. Additionally, vSAT2-93Y had significantly higher levels of IFN-γ after challenge and had a lower clinical score indicative of better protection compared to other vaccinated groups and the importance of cell mediated responses and antigen stability in protection.
Inst.de Virología
Fil: Scott, Katherine A. Agricultural Research Council. Onderstepoort Veterinary Institute. Transboundary Animal Diseases Programme; Sudáfrica. University of Pretoria. Faculty of Veterinary Science. Department of Veterinary Tropical Diseases; Sudáfrica
Fil: Rathogwa, N.M. Agricultural Research Council. Onderstepoort Veterinary Institute. Transboundary Animal Diseases Programme; Sudáfrica. University of Pretoria. Faculty of Agricultural and Natural Sciences. Department of Microbiology and Plant Pathology; Sudáfrica
Fil: Capozzo, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Maree, Francois F. Agricultural Research Council. Onderstepoort Veterinary Institute. Transboundary Animal Diseases Programme; Sudáfrica. University of Pretoria. Faculty of Agricultural and Natural Sciences. Department of Microbiology and Plant Pathology; Sudáfrica
description Foot-and-mouth disease (FMD) vaccines with improved stability and less reliant on a cold-chain are needed to improve the longevity of immune responses elicited in animals. This is especially so for serotypes O and SAT2 which are unstable in mildly acidic pH conditions or at elevated temperatures leading to dissociation of the capsid (146S particle) and loss of immunogenicity. Previously, stabilised SAT2 viruses were generated by reverse genetic approaches and assessed in vitro and in vivo with a guinea pig trial. Here we investigated the efficacy and comparative immunological responses of two thermostable and wild-type SAT2 vaccines over 5 months followed by challenge. We assessed humoral immune responses elicited in cattle in terms of total and neutralizing antibodies and IgG1/2 isotyping; and cell-mediated responses of IFN-γ as in vitro markers of protection. Whilst there were significant differences in total and neutralizing antibodies for the vSAT2-93H group compared to other vaccinated groups after the first vaccination, there were no significant differences after the second immunization. Following intra-dermolingual challenge all vaccinated groups were fully protected as determined by the absence of generalized lesions. These results provide proof that two vaccine doses, consisting of SAT2 antigen combined with ISA206B adjuvant, administered 4–6 weeks apart were able to protect animals up to 5 months pv. Additionally, vSAT2-93Y had significantly higher levels of IFN-γ after challenge and had a lower clinical score indicative of better protection compared to other vaccinated groups and the importance of cell mediated responses and antigen stability in protection.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018-03-09T16:33:26Z
2018-03-09T16:33:26Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/2007
https://www.sciencedirect.com/science/article/pii/S0264410X17301664?via%3Dihub
0264-410X
https://doi.org/10.1016/j.vaccine.2017.02.003
url http://hdl.handle.net/20.500.12123/2007
https://www.sciencedirect.com/science/article/pii/S0264410X17301664?via%3Dihub
https://doi.org/10.1016/j.vaccine.2017.02.003
identifier_str_mv 0264-410X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Vaccine 35 (40) : 5426-5433. (September 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.001348

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