Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle

Autores
Soria, Ivana; Quattrocchi, Valeria; Langellotti, Cecilia Ana; Gammella, Mariela; Digiacomo, Sebastian; Garcia de la Torre, Beatriz; Andreu, David; Montoya, Maria; Sobrino, Francisco; Blanco, Esther; Zamorano, Patricia Ines
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136–154)] linked to a T-cell epitope [3A(21–35)] of FMDV confers protection to type O FMDV challenge in pigs. Herein we show in cattle that novel dendrimeric peptides bearing a T-cell epitope [VP1(21–40] and two or four copies of a B-cell epitope [VP1(135–160)] from type O1 Campos FMDV (termed B2T and B4T, respectively) elicited FMDV specific immune responses to similar levels to a commercial vaccine. Animals were challenged with FMDV and 100% of vaccinated cattle with B2T or B4T were protected to podal generalization. Moreover, bovines immunized with B4T were completely protected (with no clinical signs) against FMDV challenge after three vaccine doses, which was associated with titers of viral neutralizing antibodies in serum higher than those of B2T group (p< 0.05) and levels of opsonic antibodies similar to those of animals immunized with one dose of FMDV commercial vaccine. Bovines vaccinated with both dendrimeric peptides presented high levels of IgG1 anti FMDV in sera and in mucosa. When IgA in nasal secretions was measured, 20% or 40% of the animals in B2T or B4T groups respectively, showed anti-FMDV IgA titers. In addition, B2T and B4T peptides evoked similar consistent T cell responses, being recognized in vitro by lymphocytes from most of the immunized cattle in the proliferation assay, and from all animals in the IFN-γ production assay. Taken together, these results support the potential of dendrimers B2T or B4T in cattle as a highly valuable, cost-effective FMDV candidate vaccine with DIVA potential.
Fil: Soria, Ivana. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gammella, Mariela. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Digiacomo, Sebastian. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Garcia de la Torre, Beatriz. Universitat Pompeu Fabra; España
Fil: Andreu, David. Universitat Pompeu Fabra; España
Fil: Montoya, Maria. The Pirbright Institute; Reino Unido
Fil: Sobrino, Francisco. Centro de Biología Molecular “Severo Ochoa”; España
Fil: Blanco, Esther. Centro de Investigación en Sanidad Animal; España
Fil: Zamorano, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Materia
DENDRIMERIC PEPTIDES
PROTECTION
CATTLE
FOOT-AND-MOUTH DISEASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41182
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/41182
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattleSoria, IvanaQuattrocchi, ValeriaLangellotti, Cecilia AnaGammella, MarielaDigiacomo, SebastianGarcia de la Torre, BeatrizAndreu, DavidMontoya, MariaSobrino, FranciscoBlanco, EstherZamorano, Patricia InesDENDRIMERIC PEPTIDESPROTECTIONCATTLEFOOT-AND-MOUTH DISEASEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136–154)] linked to a T-cell epitope [3A(21–35)] of FMDV confers protection to type O FMDV challenge in pigs. Herein we show in cattle that novel dendrimeric peptides bearing a T-cell epitope [VP1(21–40] and two or four copies of a B-cell epitope [VP1(135–160)] from type O1 Campos FMDV (termed B2T and B4T, respectively) elicited FMDV specific immune responses to similar levels to a commercial vaccine. Animals were challenged with FMDV and 100% of vaccinated cattle with B2T or B4T were protected to podal generalization. Moreover, bovines immunized with B4T were completely protected (with no clinical signs) against FMDV challenge after three vaccine doses, which was associated with titers of viral neutralizing antibodies in serum higher than those of B2T group (p< 0.05) and levels of opsonic antibodies similar to those of animals immunized with one dose of FMDV commercial vaccine. Bovines vaccinated with both dendrimeric peptides presented high levels of IgG1 anti FMDV in sera and in mucosa. When IgA in nasal secretions was measured, 20% or 40% of the animals in B2T or B4T groups respectively, showed anti-FMDV IgA titers. In addition, B2T and B4T peptides evoked similar consistent T cell responses, being recognized in vitro by lymphocytes from most of the immunized cattle in the proliferation assay, and from all animals in the IFN-γ production assay. Taken together, these results support the potential of dendrimers B2T or B4T in cattle as a highly valuable, cost-effective FMDV candidate vaccine with DIVA potential.Fil: Soria, Ivana. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gammella, Mariela. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaFil: Digiacomo, Sebastian. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaFil: Garcia de la Torre, Beatriz. Universitat Pompeu Fabra; EspañaFil: Andreu, David. Universitat Pompeu Fabra; EspañaFil: Montoya, Maria. The Pirbright Institute; Reino UnidoFil: Sobrino, Francisco. Centro de Biología Molecular “Severo Ochoa”; EspañaFil: Blanco, Esther. Centro de Investigación en Sanidad Animal; EspañaFil: Zamorano, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; ArgentinaPublic Library of Science2017-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41182Soria, Ivana; Quattrocchi, Valeria; Langellotti, Cecilia Ana; Gammella, Mariela; Digiacomo, Sebastian; et al.; Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle; Public Library of Science; Plos One; 12; 9; 9-2017; 1-16; e01851841932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0185184info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0185184info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:38:37Zoai:ri.conicet.gov.ar:11336/41182instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:38:37.828CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
title Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
spellingShingle Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
Soria, Ivana
DENDRIMERIC PEPTIDES
PROTECTION
CATTLE
FOOT-AND-MOUTH DISEASE
title_short Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
title_full Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
title_fullStr Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
title_full_unstemmed Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
title_sort Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle
dc.creator.none.fl_str_mv Soria, Ivana
Quattrocchi, Valeria
Langellotti, Cecilia Ana
Gammella, Mariela
Digiacomo, Sebastian
Garcia de la Torre, Beatriz
Andreu, David
Montoya, Maria
Sobrino, Francisco
Blanco, Esther
Zamorano, Patricia Ines
author Soria, Ivana
author_facet Soria, Ivana
Quattrocchi, Valeria
Langellotti, Cecilia Ana
Gammella, Mariela
Digiacomo, Sebastian
Garcia de la Torre, Beatriz
Andreu, David
Montoya, Maria
Sobrino, Francisco
Blanco, Esther
Zamorano, Patricia Ines
author_role author
author2 Quattrocchi, Valeria
Langellotti, Cecilia Ana
Gammella, Mariela
Digiacomo, Sebastian
Garcia de la Torre, Beatriz
Andreu, David
Montoya, Maria
Sobrino, Francisco
Blanco, Esther
Zamorano, Patricia Ines
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DENDRIMERIC PEPTIDES
PROTECTION
CATTLE
FOOT-AND-MOUTH DISEASE
topic DENDRIMERIC PEPTIDES
PROTECTION
CATTLE
FOOT-AND-MOUTH DISEASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136–154)] linked to a T-cell epitope [3A(21–35)] of FMDV confers protection to type O FMDV challenge in pigs. Herein we show in cattle that novel dendrimeric peptides bearing a T-cell epitope [VP1(21–40] and two or four copies of a B-cell epitope [VP1(135–160)] from type O1 Campos FMDV (termed B2T and B4T, respectively) elicited FMDV specific immune responses to similar levels to a commercial vaccine. Animals were challenged with FMDV and 100% of vaccinated cattle with B2T or B4T were protected to podal generalization. Moreover, bovines immunized with B4T were completely protected (with no clinical signs) against FMDV challenge after three vaccine doses, which was associated with titers of viral neutralizing antibodies in serum higher than those of B2T group (p< 0.05) and levels of opsonic antibodies similar to those of animals immunized with one dose of FMDV commercial vaccine. Bovines vaccinated with both dendrimeric peptides presented high levels of IgG1 anti FMDV in sera and in mucosa. When IgA in nasal secretions was measured, 20% or 40% of the animals in B2T or B4T groups respectively, showed anti-FMDV IgA titers. In addition, B2T and B4T peptides evoked similar consistent T cell responses, being recognized in vitro by lymphocytes from most of the immunized cattle in the proliferation assay, and from all animals in the IFN-γ production assay. Taken together, these results support the potential of dendrimers B2T or B4T in cattle as a highly valuable, cost-effective FMDV candidate vaccine with DIVA potential.
Fil: Soria, Ivana. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gammella, Mariela. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Digiacomo, Sebastian. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
Fil: Garcia de la Torre, Beatriz. Universitat Pompeu Fabra; España
Fil: Andreu, David. Universitat Pompeu Fabra; España
Fil: Montoya, Maria. The Pirbright Institute; Reino Unido
Fil: Sobrino, Francisco. Centro de Biología Molecular “Severo Ochoa”; España
Fil: Blanco, Esther. Centro de Investigación en Sanidad Animal; España
Fil: Zamorano, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires; Argentina
description Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals. A synthetic vaccine candidate consisting of dendrimeric peptides harbouring two copies of a B-epitope [VP1(136–154)] linked to a T-cell epitope [3A(21–35)] of FMDV confers protection to type O FMDV challenge in pigs. Herein we show in cattle that novel dendrimeric peptides bearing a T-cell epitope [VP1(21–40] and two or four copies of a B-cell epitope [VP1(135–160)] from type O1 Campos FMDV (termed B2T and B4T, respectively) elicited FMDV specific immune responses to similar levels to a commercial vaccine. Animals were challenged with FMDV and 100% of vaccinated cattle with B2T or B4T were protected to podal generalization. Moreover, bovines immunized with B4T were completely protected (with no clinical signs) against FMDV challenge after three vaccine doses, which was associated with titers of viral neutralizing antibodies in serum higher than those of B2T group (p< 0.05) and levels of opsonic antibodies similar to those of animals immunized with one dose of FMDV commercial vaccine. Bovines vaccinated with both dendrimeric peptides presented high levels of IgG1 anti FMDV in sera and in mucosa. When IgA in nasal secretions was measured, 20% or 40% of the animals in B2T or B4T groups respectively, showed anti-FMDV IgA titers. In addition, B2T and B4T peptides evoked similar consistent T cell responses, being recognized in vitro by lymphocytes from most of the immunized cattle in the proliferation assay, and from all animals in the IFN-γ production assay. Taken together, these results support the potential of dendrimers B2T or B4T in cattle as a highly valuable, cost-effective FMDV candidate vaccine with DIVA potential.
publishDate 2017
dc.date.none.fl_str_mv 2017-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41182
Soria, Ivana; Quattrocchi, Valeria; Langellotti, Cecilia Ana; Gammella, Mariela; Digiacomo, Sebastian; et al.; Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle; Public Library of Science; Plos One; 12; 9; 9-2017; 1-16; e0185184
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41182
identifier_str_mv Soria, Ivana; Quattrocchi, Valeria; Langellotti, Cecilia Ana; Gammella, Mariela; Digiacomo, Sebastian; et al.; Dendrimeric peptides can confer protection against foot-and-mouth disease virus in cattle; Public Library of Science; Plos One; 12; 9; 9-2017; 1-16; e0185184
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0185184
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0185184
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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