New validated eucalyptus SSR markers located in candidate genes involved in growth and plant development

Autores
Acuña, Cintia Vanesa; Rivas, Juan Gabriel; Aguirre, Natalia Cristina; Villalba, Pamela Victoria; Martinez, Maria Carolina; Garcia, Martin Nahuel; Hopp, Horacio Esteban; Marcucci Poltri, Susana Noemi
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aim of study: To validate and characterize new microsatellites or Simple Sequence Repeats (SSR) markers, located within genomic transcribed sequences related to growth and plant developmental traits, in Eucalyptus species. Area of study: Eucalyptus species from different Australian origins planted in Argentina. Materials and methods: In total, 134 SSR in 129 candidate genes (CG-SSR) involved in plant development were selected and physically mapped to the E. grandis reference genome by bioinformatic tools. Experimental validation and polymorphism analysis were performed on 48 individuals from E. grandis and interspecific hybrids (E. grandis x E. camaldulensis; E. grandis x E. tereticornis), E. globulus, E. maidenii, E. dunnii and E. benthamii. Main results: 131 out of 134 CG-SSR were mapped on the 11 chromosomes of E. grandis reference genome. Most of the 134 analyzed SSR (> 75%) were positively amplified and 39 were polymorphic in at least one species. A search of annotated genes within a 25 kbp up and downstream region of each SSR location retrieved 773 genes of interest. Research highlights: The new validated and characterized CG-SSR are potentially suitable for comparative QTL mapping, molecular marker-assisted breeding (MAB) and population genetic studies across different species within Symphyomyrtus subgenus.
Instituto de Biotecnología
Fil: Acuña, Cintia Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Acuña, Cintia Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rivas, Juan Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Rivas, Juan Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Aguirre, Natalia Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Aguirre, Natalia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Villalba, Pamela Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Villalba, Pamela Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martinez, Maria Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Martinez, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Garcia, Martin Nahuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Garcia, Martin Nahuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Hopp, Horacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hopp, Horacio Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Marcucci Poltri, Susana Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Marcucci Poltri, Susana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Forest Systems 29 (3) : eSC08 (2020)
Materia
Eucalyptus
Microsatélites
Crecimiento
Microsatellites
Growth
Genetic Markers
Marcadores Genéticos
Candidate Genes
Plant Development
Genes Candidatos
Desarrollo de Plantas
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/12133
Acceder
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oai_identifier_str oai:localhost:20.500.12123/12133
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network_name_str INTA Digital (INTA)
spelling New validated eucalyptus SSR markers located in candidate genes involved in growth and plant developmentAcuña, Cintia VanesaRivas, Juan GabrielAguirre, Natalia CristinaVillalba, Pamela VictoriaMartinez, Maria CarolinaGarcia, Martin NahuelHopp, Horacio EstebanMarcucci Poltri, Susana NoemiEucalyptusMicrosatélitesCrecimientoMicrosatellitesGrowthGenetic MarkersMarcadores GenéticosCandidate GenesPlant DevelopmentGenes CandidatosDesarrollo de PlantasAim of study: To validate and characterize new microsatellites or Simple Sequence Repeats (SSR) markers, located within genomic transcribed sequences related to growth and plant developmental traits, in Eucalyptus species. Area of study: Eucalyptus species from different Australian origins planted in Argentina. Materials and methods: In total, 134 SSR in 129 candidate genes (CG-SSR) involved in plant development were selected and physically mapped to the E. grandis reference genome by bioinformatic tools. Experimental validation and polymorphism analysis were performed on 48 individuals from E. grandis and interspecific hybrids (E. grandis x E. camaldulensis; E. grandis x E. tereticornis), E. globulus, E. maidenii, E. dunnii and E. benthamii. Main results: 131 out of 134 CG-SSR were mapped on the 11 chromosomes of E. grandis reference genome. Most of the 134 analyzed SSR (> 75%) were positively amplified and 39 were polymorphic in at least one species. A search of annotated genes within a 25 kbp up and downstream region of each SSR location retrieved 773 genes of interest. Research highlights: The new validated and characterized CG-SSR are potentially suitable for comparative QTL mapping, molecular marker-assisted breeding (MAB) and population genetic studies across different species within Symphyomyrtus subgenus.Instituto de BiotecnologíaFil: Acuña, Cintia Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Acuña, Cintia Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rivas, Juan Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Rivas, Juan Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Aguirre, Natalia Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Aguirre, Natalia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Villalba, Pamela Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Villalba, Pamela Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Martinez, Maria Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Martinez, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Garcia, Martin Nahuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Garcia, Martin Nahuel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Hopp, Horacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hopp, Horacio Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Marcucci Poltri, Susana Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Marcucci Poltri, Susana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaInstituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España)2022-06-22T10:17:48Z2022-06-22T10:17:48Z2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/12133https://revistas.inia.es/index.php/fs/article/view/170742171-9845https://doi.org/10.5424/fs/2020293-17074Forest Systems 29 (3) : eSC08 (2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNFOR-1104064/AR./Aplicación de herramientas moleculares para el uso y la conservación de la diversidad genética forestal.info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:35Zoai:localhost:20.500.12123/12133instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:36.09INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv New validated eucalyptus SSR markers located in candidate genes involved in growth and plant development
title New validated eucalyptus SSR markers located in candidate genes involved in growth and plant development
spellingShingle New validated eucalyptus SSR markers located in candidate genes involved in growth and plant development
Acuña, Cintia Vanesa
Eucalyptus
Microsatélites
Crecimiento
Microsatellites
Growth
Genetic Markers
Marcadores Genéticos
Candidate Genes
Plant Development
Genes Candidatos
Desarrollo de Plantas
title_short New validated eucalyptus SSR markers located in candidate genes involved in growth and plant development
title_full New validated eucalyptus SSR markers located in candidate genes involved in growth and plant development
title_fullStr New validated eucalyptus SSR markers located in candidate genes involved in growth and plant development
title_full_unstemmed New validated eucalyptus SSR markers located in candidate genes involved in growth and plant development
title_sort New validated eucalyptus SSR markers located in candidate genes involved in growth and plant development
dc.creator.none.fl_str_mv Acuña, Cintia Vanesa
Rivas, Juan Gabriel
Aguirre, Natalia Cristina
Villalba, Pamela Victoria
Martinez, Maria Carolina
Garcia, Martin Nahuel
Hopp, Horacio Esteban
Marcucci Poltri, Susana Noemi
author Acuña, Cintia Vanesa
author_facet Acuña, Cintia Vanesa
Rivas, Juan Gabriel
Aguirre, Natalia Cristina
Villalba, Pamela Victoria
Martinez, Maria Carolina
Garcia, Martin Nahuel
Hopp, Horacio Esteban
Marcucci Poltri, Susana Noemi
author_role author
author2 Rivas, Juan Gabriel
Aguirre, Natalia Cristina
Villalba, Pamela Victoria
Martinez, Maria Carolina
Garcia, Martin Nahuel
Hopp, Horacio Esteban
Marcucci Poltri, Susana Noemi
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Eucalyptus
Microsatélites
Crecimiento
Microsatellites
Growth
Genetic Markers
Marcadores Genéticos
Candidate Genes
Plant Development
Genes Candidatos
Desarrollo de Plantas
topic Eucalyptus
Microsatélites
Crecimiento
Microsatellites
Growth
Genetic Markers
Marcadores Genéticos
Candidate Genes
Plant Development
Genes Candidatos
Desarrollo de Plantas
dc.description.none.fl_txt_mv Aim of study: To validate and characterize new microsatellites or Simple Sequence Repeats (SSR) markers, located within genomic transcribed sequences related to growth and plant developmental traits, in Eucalyptus species. Area of study: Eucalyptus species from different Australian origins planted in Argentina. Materials and methods: In total, 134 SSR in 129 candidate genes (CG-SSR) involved in plant development were selected and physically mapped to the E. grandis reference genome by bioinformatic tools. Experimental validation and polymorphism analysis were performed on 48 individuals from E. grandis and interspecific hybrids (E. grandis x E. camaldulensis; E. grandis x E. tereticornis), E. globulus, E. maidenii, E. dunnii and E. benthamii. Main results: 131 out of 134 CG-SSR were mapped on the 11 chromosomes of E. grandis reference genome. Most of the 134 analyzed SSR (> 75%) were positively amplified and 39 were polymorphic in at least one species. A search of annotated genes within a 25 kbp up and downstream region of each SSR location retrieved 773 genes of interest. Research highlights: The new validated and characterized CG-SSR are potentially suitable for comparative QTL mapping, molecular marker-assisted breeding (MAB) and population genetic studies across different species within Symphyomyrtus subgenus.
Instituto de Biotecnología
Fil: Acuña, Cintia Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Acuña, Cintia Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rivas, Juan Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Rivas, Juan Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Aguirre, Natalia Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Aguirre, Natalia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Villalba, Pamela Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Villalba, Pamela Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martinez, Maria Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Martinez, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Garcia, Martin Nahuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Garcia, Martin Nahuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Hopp, Horacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hopp, Horacio Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Marcucci Poltri, Susana Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Marcucci Poltri, Susana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Aim of study: To validate and characterize new microsatellites or Simple Sequence Repeats (SSR) markers, located within genomic transcribed sequences related to growth and plant developmental traits, in Eucalyptus species. Area of study: Eucalyptus species from different Australian origins planted in Argentina. Materials and methods: In total, 134 SSR in 129 candidate genes (CG-SSR) involved in plant development were selected and physically mapped to the E. grandis reference genome by bioinformatic tools. Experimental validation and polymorphism analysis were performed on 48 individuals from E. grandis and interspecific hybrids (E. grandis x E. camaldulensis; E. grandis x E. tereticornis), E. globulus, E. maidenii, E. dunnii and E. benthamii. Main results: 131 out of 134 CG-SSR were mapped on the 11 chromosomes of E. grandis reference genome. Most of the 134 analyzed SSR (> 75%) were positively amplified and 39 were polymorphic in at least one species. A search of annotated genes within a 25 kbp up and downstream region of each SSR location retrieved 773 genes of interest. Research highlights: The new validated and characterized CG-SSR are potentially suitable for comparative QTL mapping, molecular marker-assisted breeding (MAB) and population genetic studies across different species within Symphyomyrtus subgenus.
publishDate 2020
dc.date.none.fl_str_mv 2020
2022-06-22T10:17:48Z
2022-06-22T10:17:48Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/12133
https://revistas.inia.es/index.php/fs/article/view/17074
2171-9845
https://doi.org/10.5424/fs/2020293-17074
url http://hdl.handle.net/20.500.12123/12133
https://revistas.inia.es/index.php/fs/article/view/17074
https://doi.org/10.5424/fs/2020293-17074
identifier_str_mv 2171-9845
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNFOR-1104064/AR./Aplicación de herramientas moleculares para el uso y la conservación de la diversidad genética forestal.
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España)
publisher.none.fl_str_mv Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España)
dc.source.none.fl_str_mv Forest Systems 29 (3) : eSC08 (2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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