Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species

Autores
Arizio, Carla Marcela; Costa Tártara, Sabrina María; Zunino, Ignacio; Manifesto, Maria Marcela
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The anthocyanins are pigments responsible for a wide range of colours in plants, from blue, red and purple, play essential biological roles as well as their genes are evolutionarily conserved. Purple sweet potatoes have anthocyanins as the predominant colour, even though they are present in orange roots masked by carotenoids. Several studies have focused on molecular aspects of anthocyanin genes, mainly in wild Ipomoea species, although the structure and segregation analysis of those genes in sweet potato hexaploid species are still unknown. Based on an “exon-primed intron crossing” (EPIC) approach, fourteen pairs of primers were designed, on fi ve structural anthocyanin genes as candidates. The strategy exploits the Intron Length Polymorphism (ILP) from Candidate Genes (CG), resulting in 93% of successful markers giving scorable and reproducible alleles. The results allowed to defi ne partial structure and sequence of the introns and exons from the selected CG, and to determine patterns of sequence variation. The evaluation of marker dosage and allelic segregations in an Ipomoea batatas (L.) Lam mapping population identifi ed several alleles for linkage analysis. The study validated the utility of ILP-CG markers for genetic diversity and conservation applicability and a successful amplifi cation gradient across wild Ipomoea species validated their transferability
Fil: Arizio, Carla Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Costa Tártara, Sabrina María. Universidad Nacional de Luján; Argentina
Fil: Zunino, Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Agencia de Extensión Rural Mercedes; Argentina
Fil: Manifesto, Maria Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fuente
Anais da Academia Brasileira de Ciencias 94 (3) : e20210672 (2022)
Materia
Anthocyanins
Candidate Genes
Genetic Diversity (as resource)
Genetic Markers
Antocianinas
Genes Candidatos
Diversidad Genética (como recurso)
Ipomoea
Marcadores Genéticos
ILP-CG Markers
Wild Ipomoeas
Marcadores ILP-CG
Ipomoeas Salvajes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/13679
Acceder
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oai_identifier_str oai:localhost:20.500.12123/13679
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network_name_str INTA Digital (INTA)
spelling Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea speciesArizio, Carla MarcelaCosta Tártara, Sabrina MaríaZunino, IgnacioManifesto, Maria MarcelaAnthocyaninsCandidate GenesGenetic Diversity (as resource)Genetic MarkersAntocianinasGenes CandidatosDiversidad Genética (como recurso)IpomoeaMarcadores GenéticosILP-CG MarkersWild IpomoeasMarcadores ILP-CGIpomoeas SalvajesThe anthocyanins are pigments responsible for a wide range of colours in plants, from blue, red and purple, play essential biological roles as well as their genes are evolutionarily conserved. Purple sweet potatoes have anthocyanins as the predominant colour, even though they are present in orange roots masked by carotenoids. Several studies have focused on molecular aspects of anthocyanin genes, mainly in wild Ipomoea species, although the structure and segregation analysis of those genes in sweet potato hexaploid species are still unknown. Based on an “exon-primed intron crossing” (EPIC) approach, fourteen pairs of primers were designed, on fi ve structural anthocyanin genes as candidates. The strategy exploits the Intron Length Polymorphism (ILP) from Candidate Genes (CG), resulting in 93% of successful markers giving scorable and reproducible alleles. The results allowed to defi ne partial structure and sequence of the introns and exons from the selected CG, and to determine patterns of sequence variation. The evaluation of marker dosage and allelic segregations in an Ipomoea batatas (L.) Lam mapping population identifi ed several alleles for linkage analysis. The study validated the utility of ILP-CG markers for genetic diversity and conservation applicability and a successful amplifi cation gradient across wild Ipomoea species validated their transferabilityFil: Arizio, Carla Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; ArgentinaFil: Costa Tártara, Sabrina María. Universidad Nacional de Luján; ArgentinaFil: Zunino, Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Agencia de Extensión Rural Mercedes; ArgentinaFil: Manifesto, Maria Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; ArgentinaAcademia Brasileira de Ciencias2022-12-22T10:22:13Z2022-12-22T10:22:13Z2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/13679https://www.scielo.br/j/aabc/a/c4KdzQ6PfcMs7SzyxHxHdMm/?lang=en0001-37651678-2690https://doi.org/10.1590/0001-3765202220210672Anais da Academia Brasileira de Ciencias 94 (3) : e20210672 (2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:31:02Zoai:localhost:20.500.12123/13679instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:31:02.414INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species
title Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species
spellingShingle Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species
Arizio, Carla Marcela
Anthocyanins
Candidate Genes
Genetic Diversity (as resource)
Genetic Markers
Antocianinas
Genes Candidatos
Diversidad Genética (como recurso)
Ipomoea
Marcadores Genéticos
ILP-CG Markers
Wild Ipomoeas
Marcadores ILP-CG
Ipomoeas Salvajes
title_short Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species
title_full Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species
title_fullStr Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species
title_full_unstemmed Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species
title_sort Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species
dc.creator.none.fl_str_mv Arizio, Carla Marcela
Costa Tártara, Sabrina María
Zunino, Ignacio
Manifesto, Maria Marcela
author Arizio, Carla Marcela
author_facet Arizio, Carla Marcela
Costa Tártara, Sabrina María
Zunino, Ignacio
Manifesto, Maria Marcela
author_role author
author2 Costa Tártara, Sabrina María
Zunino, Ignacio
Manifesto, Maria Marcela
author2_role author
author
author
dc.subject.none.fl_str_mv Anthocyanins
Candidate Genes
Genetic Diversity (as resource)
Genetic Markers
Antocianinas
Genes Candidatos
Diversidad Genética (como recurso)
Ipomoea
Marcadores Genéticos
ILP-CG Markers
Wild Ipomoeas
Marcadores ILP-CG
Ipomoeas Salvajes
topic Anthocyanins
Candidate Genes
Genetic Diversity (as resource)
Genetic Markers
Antocianinas
Genes Candidatos
Diversidad Genética (como recurso)
Ipomoea
Marcadores Genéticos
ILP-CG Markers
Wild Ipomoeas
Marcadores ILP-CG
Ipomoeas Salvajes
dc.description.none.fl_txt_mv The anthocyanins are pigments responsible for a wide range of colours in plants, from blue, red and purple, play essential biological roles as well as their genes are evolutionarily conserved. Purple sweet potatoes have anthocyanins as the predominant colour, even though they are present in orange roots masked by carotenoids. Several studies have focused on molecular aspects of anthocyanin genes, mainly in wild Ipomoea species, although the structure and segregation analysis of those genes in sweet potato hexaploid species are still unknown. Based on an “exon-primed intron crossing” (EPIC) approach, fourteen pairs of primers were designed, on fi ve structural anthocyanin genes as candidates. The strategy exploits the Intron Length Polymorphism (ILP) from Candidate Genes (CG), resulting in 93% of successful markers giving scorable and reproducible alleles. The results allowed to defi ne partial structure and sequence of the introns and exons from the selected CG, and to determine patterns of sequence variation. The evaluation of marker dosage and allelic segregations in an Ipomoea batatas (L.) Lam mapping population identifi ed several alleles for linkage analysis. The study validated the utility of ILP-CG markers for genetic diversity and conservation applicability and a successful amplifi cation gradient across wild Ipomoea species validated their transferability
Fil: Arizio, Carla Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Costa Tártara, Sabrina María. Universidad Nacional de Luján; Argentina
Fil: Zunino, Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Agencia de Extensión Rural Mercedes; Argentina
Fil: Manifesto, Maria Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
description The anthocyanins are pigments responsible for a wide range of colours in plants, from blue, red and purple, play essential biological roles as well as their genes are evolutionarily conserved. Purple sweet potatoes have anthocyanins as the predominant colour, even though they are present in orange roots masked by carotenoids. Several studies have focused on molecular aspects of anthocyanin genes, mainly in wild Ipomoea species, although the structure and segregation analysis of those genes in sweet potato hexaploid species are still unknown. Based on an “exon-primed intron crossing” (EPIC) approach, fourteen pairs of primers were designed, on fi ve structural anthocyanin genes as candidates. The strategy exploits the Intron Length Polymorphism (ILP) from Candidate Genes (CG), resulting in 93% of successful markers giving scorable and reproducible alleles. The results allowed to defi ne partial structure and sequence of the introns and exons from the selected CG, and to determine patterns of sequence variation. The evaluation of marker dosage and allelic segregations in an Ipomoea batatas (L.) Lam mapping population identifi ed several alleles for linkage analysis. The study validated the utility of ILP-CG markers for genetic diversity and conservation applicability and a successful amplifi cation gradient across wild Ipomoea species validated their transferability
publishDate 2022
dc.date.none.fl_str_mv 2022-12-22T10:22:13Z
2022-12-22T10:22:13Z
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/13679
https://www.scielo.br/j/aabc/a/c4KdzQ6PfcMs7SzyxHxHdMm/?lang=en
0001-3765
1678-2690
https://doi.org/10.1590/0001-3765202220210672
url http://hdl.handle.net/20.500.12123/13679
https://www.scielo.br/j/aabc/a/c4KdzQ6PfcMs7SzyxHxHdMm/?lang=en
https://doi.org/10.1590/0001-3765202220210672
identifier_str_mv 0001-3765
1678-2690
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Academia Brasileira de Ciencias
publisher.none.fl_str_mv Academia Brasileira de Ciencias
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciencias 94 (3) : e20210672 (2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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