Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species
- Autores
- Arizio, Carla Marcela; Costa Tártara, Sabrina María; Zunino, Ignacio; Manifesto, Maria Marcela
- Año de publicación
- 2022
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The anthocyanins are pigments responsible for a wide range of colours in plants, from blue, red and purple, play essential biological roles as well as their genes are evolutionarily conserved. Purple sweet potatoes have anthocyanins as the predominant colour, even though they are present in orange roots masked by carotenoids. Several studies have focused on molecular aspects of anthocyanin genes, mainly in wild Ipomoea species, although the structure and segregation analysis of those genes in sweet potato hexaploid species are still unknown. Based on an “exon-primed intron crossing” (EPIC) approach, fourteen pairs of primers were designed, on fi ve structural anthocyanin genes as candidates. The strategy exploits the Intron Length Polymorphism (ILP) from Candidate Genes (CG), resulting in 93% of successful markers giving scorable and reproducible alleles. The results allowed to defi ne partial structure and sequence of the introns and exons from the selected CG, and to determine patterns of sequence variation. The evaluation of marker dosage and allelic segregations in an Ipomoea batatas (L.) Lam mapping population identifi ed several alleles for linkage analysis. The study validated the utility of ILP-CG markers for genetic diversity and conservation applicability and a successful amplifi cation gradient across wild Ipomoea species validated their transferability
Fil: Arizio, Carla Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Costa Tártara, Sabrina María. Universidad Nacional de Luján; Argentina
Fil: Zunino, Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Agencia de Extensión Rural Mercedes; Argentina
Fil: Manifesto, Maria Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina - Fuente
- Anais da Academia Brasileira de Ciencias 94 (3) : e20210672 (2022)
- Materia
-
Anthocyanins
Candidate Genes
Genetic Diversity (as resource)
Genetic Markers
Antocianinas
Genes Candidatos
Diversidad Genética (como recurso)
Ipomoea
Marcadores Genéticos
ILP-CG Markers
Wild Ipomoeas
Marcadores ILP-CG
Ipomoeas Salvajes - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/13679
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Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea speciesArizio, Carla MarcelaCosta Tártara, Sabrina MaríaZunino, IgnacioManifesto, Maria MarcelaAnthocyaninsCandidate GenesGenetic Diversity (as resource)Genetic MarkersAntocianinasGenes CandidatosDiversidad Genética (como recurso)IpomoeaMarcadores GenéticosILP-CG MarkersWild IpomoeasMarcadores ILP-CGIpomoeas SalvajesThe anthocyanins are pigments responsible for a wide range of colours in plants, from blue, red and purple, play essential biological roles as well as their genes are evolutionarily conserved. Purple sweet potatoes have anthocyanins as the predominant colour, even though they are present in orange roots masked by carotenoids. Several studies have focused on molecular aspects of anthocyanin genes, mainly in wild Ipomoea species, although the structure and segregation analysis of those genes in sweet potato hexaploid species are still unknown. Based on an “exon-primed intron crossing” (EPIC) approach, fourteen pairs of primers were designed, on fi ve structural anthocyanin genes as candidates. The strategy exploits the Intron Length Polymorphism (ILP) from Candidate Genes (CG), resulting in 93% of successful markers giving scorable and reproducible alleles. The results allowed to defi ne partial structure and sequence of the introns and exons from the selected CG, and to determine patterns of sequence variation. The evaluation of marker dosage and allelic segregations in an Ipomoea batatas (L.) Lam mapping population identifi ed several alleles for linkage analysis. The study validated the utility of ILP-CG markers for genetic diversity and conservation applicability and a successful amplifi cation gradient across wild Ipomoea species validated their transferabilityFil: Arizio, Carla Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; ArgentinaFil: Costa Tártara, Sabrina María. Universidad Nacional de Luján; ArgentinaFil: Zunino, Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Agencia de Extensión Rural Mercedes; ArgentinaFil: Manifesto, Maria Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; ArgentinaAcademia Brasileira de Ciencias2022-12-22T10:22:13Z2022-12-22T10:22:13Z2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/13679https://www.scielo.br/j/aabc/a/c4KdzQ6PfcMs7SzyxHxHdMm/?lang=en0001-37651678-2690https://doi.org/10.1590/0001-3765202220210672Anais da Academia Brasileira de Ciencias 94 (3) : e20210672 (2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:50Zoai:localhost:20.500.12123/13679instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:50.483INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species |
title |
Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species |
spellingShingle |
Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species Arizio, Carla Marcela Anthocyanins Candidate Genes Genetic Diversity (as resource) Genetic Markers Antocianinas Genes Candidatos Diversidad Genética (como recurso) Ipomoea Marcadores Genéticos ILP-CG Markers Wild Ipomoeas Marcadores ILP-CG Ipomoeas Salvajes |
title_short |
Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species |
title_full |
Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species |
title_fullStr |
Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species |
title_full_unstemmed |
Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species |
title_sort |
Development of anthocyanin markers: gene mapping, genomic analysis and genetic diversity studies in Ipomoea species |
dc.creator.none.fl_str_mv |
Arizio, Carla Marcela Costa Tártara, Sabrina María Zunino, Ignacio Manifesto, Maria Marcela |
author |
Arizio, Carla Marcela |
author_facet |
Arizio, Carla Marcela Costa Tártara, Sabrina María Zunino, Ignacio Manifesto, Maria Marcela |
author_role |
author |
author2 |
Costa Tártara, Sabrina María Zunino, Ignacio Manifesto, Maria Marcela |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Anthocyanins Candidate Genes Genetic Diversity (as resource) Genetic Markers Antocianinas Genes Candidatos Diversidad Genética (como recurso) Ipomoea Marcadores Genéticos ILP-CG Markers Wild Ipomoeas Marcadores ILP-CG Ipomoeas Salvajes |
topic |
Anthocyanins Candidate Genes Genetic Diversity (as resource) Genetic Markers Antocianinas Genes Candidatos Diversidad Genética (como recurso) Ipomoea Marcadores Genéticos ILP-CG Markers Wild Ipomoeas Marcadores ILP-CG Ipomoeas Salvajes |
dc.description.none.fl_txt_mv |
The anthocyanins are pigments responsible for a wide range of colours in plants, from blue, red and purple, play essential biological roles as well as their genes are evolutionarily conserved. Purple sweet potatoes have anthocyanins as the predominant colour, even though they are present in orange roots masked by carotenoids. Several studies have focused on molecular aspects of anthocyanin genes, mainly in wild Ipomoea species, although the structure and segregation analysis of those genes in sweet potato hexaploid species are still unknown. Based on an “exon-primed intron crossing” (EPIC) approach, fourteen pairs of primers were designed, on fi ve structural anthocyanin genes as candidates. The strategy exploits the Intron Length Polymorphism (ILP) from Candidate Genes (CG), resulting in 93% of successful markers giving scorable and reproducible alleles. The results allowed to defi ne partial structure and sequence of the introns and exons from the selected CG, and to determine patterns of sequence variation. The evaluation of marker dosage and allelic segregations in an Ipomoea batatas (L.) Lam mapping population identifi ed several alleles for linkage analysis. The study validated the utility of ILP-CG markers for genetic diversity and conservation applicability and a successful amplifi cation gradient across wild Ipomoea species validated their transferability Fil: Arizio, Carla Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Costa Tártara, Sabrina María. Universidad Nacional de Luján; Argentina Fil: Zunino, Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Agencia de Extensión Rural Mercedes; Argentina Fil: Manifesto, Maria Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina |
description |
The anthocyanins are pigments responsible for a wide range of colours in plants, from blue, red and purple, play essential biological roles as well as their genes are evolutionarily conserved. Purple sweet potatoes have anthocyanins as the predominant colour, even though they are present in orange roots masked by carotenoids. Several studies have focused on molecular aspects of anthocyanin genes, mainly in wild Ipomoea species, although the structure and segregation analysis of those genes in sweet potato hexaploid species are still unknown. Based on an “exon-primed intron crossing” (EPIC) approach, fourteen pairs of primers were designed, on fi ve structural anthocyanin genes as candidates. The strategy exploits the Intron Length Polymorphism (ILP) from Candidate Genes (CG), resulting in 93% of successful markers giving scorable and reproducible alleles. The results allowed to defi ne partial structure and sequence of the introns and exons from the selected CG, and to determine patterns of sequence variation. The evaluation of marker dosage and allelic segregations in an Ipomoea batatas (L.) Lam mapping population identifi ed several alleles for linkage analysis. The study validated the utility of ILP-CG markers for genetic diversity and conservation applicability and a successful amplifi cation gradient across wild Ipomoea species validated their transferability |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-22T10:22:13Z 2022-12-22T10:22:13Z 2022 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/13679 https://www.scielo.br/j/aabc/a/c4KdzQ6PfcMs7SzyxHxHdMm/?lang=en 0001-3765 1678-2690 https://doi.org/10.1590/0001-3765202220210672 |
url |
http://hdl.handle.net/20.500.12123/13679 https://www.scielo.br/j/aabc/a/c4KdzQ6PfcMs7SzyxHxHdMm/?lang=en https://doi.org/10.1590/0001-3765202220210672 |
identifier_str_mv |
0001-3765 1678-2690 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Academia Brasileira de Ciencias |
publisher.none.fl_str_mv |
Academia Brasileira de Ciencias |
dc.source.none.fl_str_mv |
Anais da Academia Brasileira de Ciencias 94 (3) : e20210672 (2022) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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1844619172674273280 |
score |
12.559606 |