Evaluation of bradyrhizobia strains isolated from field-grown soybean plants in Argentina as improved inoculants

Autores
Melchiorre, Mariana; De Luca, Marcos Javier; Gonzalez Anta, Gustavo; Suarez, Paola Alejandra; Lopez, Carlos; Lascano, Hernan Ramiro; Racca, Roberto Walter
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bradyrhizobium strains were isolated from nodules obtained from field-grown soybean plants sampled in 12 soybean production locations in Argentina. These fields had been annually cropped with soybean and did not show decreases in yields even though they had been neither N-fertilized nor inoculated for at least the last 5 years. We hypothesized that the isolated strains maintained high competitiveness and efficiency in fixing adequate N2 levels. A set of strains that showed the highest nodular occupancy in each sampling location were assayed for symbiotic performance under greenhouse and field conditions and comparatively evaluated with Bradyrhizobium japonicum E109, the strain officially recommended for inoculant formulation in Argentina. An inoculant pool, formed by four strains obtained from nodules collected from Cañada Rica, developed higher nodular biomass than B. japonicum E 109 in assays carried out in greenhouses under well irrigated conditions. Additionally, neither nodule production nor specific nitrogenase activity decreased with respect to B. japonicum E 109 when plants were drought stressed during 7 days from sowing. The mean yields obtained under field conditions and plotted against the principal component one (CP1) obtained with an additive main effect and multiplicative interaction (AMMI) model showed that the inoculant pool from Cañada Rica had higher contribution to yield than strain E 109, although with lower environmental stability. The inoculant pool from Cañada Rica could be considered an improved inoculant and be used for preliminary assays, to formulate inoculants in Argentina.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Melchiorre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: De Luca, Marcos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Gonzalez Anta, Gustavo. Universidad Nacional del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Suarez, Paola Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Lopez, Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi. Agencia De Extensión Rural Río Primero; Argentina
Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Racca, Roberto Walter. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fuente
Biology and Fertility of Soils 47 : 81–89 (2011)
Materia
Bradyrhizobium japonicum
Soja
Argentina
Biological Nitrogen Fixation
Soybeans
Soja
Fijación Biológica del Nitrógeno
Inoculant
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10934
Acceder
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oai_identifier_str oai:localhost:20.500.12123/10934
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Evaluation of bradyrhizobia strains isolated from field-grown soybean plants in Argentina as improved inoculantsMelchiorre, MarianaDe Luca, Marcos JavierGonzalez Anta, GustavoSuarez, Paola AlejandraLopez, CarlosLascano, Hernan RamiroRacca, Roberto WalterBradyrhizobium japonicumSojaArgentinaBiological Nitrogen FixationSoybeansSojaFijación Biológica del NitrógenoInoculantBradyrhizobium strains were isolated from nodules obtained from field-grown soybean plants sampled in 12 soybean production locations in Argentina. These fields had been annually cropped with soybean and did not show decreases in yields even though they had been neither N-fertilized nor inoculated for at least the last 5 years. We hypothesized that the isolated strains maintained high competitiveness and efficiency in fixing adequate N2 levels. A set of strains that showed the highest nodular occupancy in each sampling location were assayed for symbiotic performance under greenhouse and field conditions and comparatively evaluated with Bradyrhizobium japonicum E109, the strain officially recommended for inoculant formulation in Argentina. An inoculant pool, formed by four strains obtained from nodules collected from Cañada Rica, developed higher nodular biomass than B. japonicum E 109 in assays carried out in greenhouses under well irrigated conditions. Additionally, neither nodule production nor specific nitrogenase activity decreased with respect to B. japonicum E 109 when plants were drought stressed during 7 days from sowing. The mean yields obtained under field conditions and plotted against the principal component one (CP1) obtained with an additive main effect and multiplicative interaction (AMMI) model showed that the inoculant pool from Cañada Rica had higher contribution to yield than strain E 109, although with lower environmental stability. The inoculant pool from Cañada Rica could be considered an improved inoculant and be used for preliminary assays, to formulate inoculants in Argentina.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Melchiorre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: De Luca, Marcos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Gonzalez Anta, Gustavo. Universidad Nacional del Noroeste de la Provincia de Buenos Aires; ArgentinaFil: Suarez, Paola Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Lopez, Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi. Agencia De Extensión Rural Río Primero; ArgentinaFil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Racca, Roberto Walter. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaSpringer2021-12-17T19:40:15Z2021-12-17T19:40:15Z2010-09-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10934https://link.springer.com/article/10.1007/s00374-010-0503-70178-27621432-0789 (online)https://doi.org/10.1007/s00374-010-0503-7Biology and Fertility of Soils 47 : 81–89 (2011)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:30:19Zoai:localhost:20.500.12123/10934instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:30:20.193INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Evaluation of bradyrhizobia strains isolated from field-grown soybean plants in Argentina as improved inoculants
title Evaluation of bradyrhizobia strains isolated from field-grown soybean plants in Argentina as improved inoculants
spellingShingle Evaluation of bradyrhizobia strains isolated from field-grown soybean plants in Argentina as improved inoculants
Melchiorre, Mariana
Bradyrhizobium japonicum
Soja
Argentina
Biological Nitrogen Fixation
Soybeans
Soja
Fijación Biológica del Nitrógeno
Inoculant
title_short Evaluation of bradyrhizobia strains isolated from field-grown soybean plants in Argentina as improved inoculants
title_full Evaluation of bradyrhizobia strains isolated from field-grown soybean plants in Argentina as improved inoculants
title_fullStr Evaluation of bradyrhizobia strains isolated from field-grown soybean plants in Argentina as improved inoculants
title_full_unstemmed Evaluation of bradyrhizobia strains isolated from field-grown soybean plants in Argentina as improved inoculants
title_sort Evaluation of bradyrhizobia strains isolated from field-grown soybean plants in Argentina as improved inoculants
dc.creator.none.fl_str_mv Melchiorre, Mariana
De Luca, Marcos Javier
Gonzalez Anta, Gustavo
Suarez, Paola Alejandra
Lopez, Carlos
Lascano, Hernan Ramiro
Racca, Roberto Walter
author Melchiorre, Mariana
author_facet Melchiorre, Mariana
De Luca, Marcos Javier
Gonzalez Anta, Gustavo
Suarez, Paola Alejandra
Lopez, Carlos
Lascano, Hernan Ramiro
Racca, Roberto Walter
author_role author
author2 De Luca, Marcos Javier
Gonzalez Anta, Gustavo
Suarez, Paola Alejandra
Lopez, Carlos
Lascano, Hernan Ramiro
Racca, Roberto Walter
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Bradyrhizobium japonicum
Soja
Argentina
Biological Nitrogen Fixation
Soybeans
Soja
Fijación Biológica del Nitrógeno
Inoculant
topic Bradyrhizobium japonicum
Soja
Argentina
Biological Nitrogen Fixation
Soybeans
Soja
Fijación Biológica del Nitrógeno
Inoculant
dc.description.none.fl_txt_mv Bradyrhizobium strains were isolated from nodules obtained from field-grown soybean plants sampled in 12 soybean production locations in Argentina. These fields had been annually cropped with soybean and did not show decreases in yields even though they had been neither N-fertilized nor inoculated for at least the last 5 years. We hypothesized that the isolated strains maintained high competitiveness and efficiency in fixing adequate N2 levels. A set of strains that showed the highest nodular occupancy in each sampling location were assayed for symbiotic performance under greenhouse and field conditions and comparatively evaluated with Bradyrhizobium japonicum E109, the strain officially recommended for inoculant formulation in Argentina. An inoculant pool, formed by four strains obtained from nodules collected from Cañada Rica, developed higher nodular biomass than B. japonicum E 109 in assays carried out in greenhouses under well irrigated conditions. Additionally, neither nodule production nor specific nitrogenase activity decreased with respect to B. japonicum E 109 when plants were drought stressed during 7 days from sowing. The mean yields obtained under field conditions and plotted against the principal component one (CP1) obtained with an additive main effect and multiplicative interaction (AMMI) model showed that the inoculant pool from Cañada Rica had higher contribution to yield than strain E 109, although with lower environmental stability. The inoculant pool from Cañada Rica could be considered an improved inoculant and be used for preliminary assays, to formulate inoculants in Argentina.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Melchiorre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: De Luca, Marcos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Gonzalez Anta, Gustavo. Universidad Nacional del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Suarez, Paola Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Lopez, Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi. Agencia De Extensión Rural Río Primero; Argentina
Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Racca, Roberto Walter. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
description Bradyrhizobium strains were isolated from nodules obtained from field-grown soybean plants sampled in 12 soybean production locations in Argentina. These fields had been annually cropped with soybean and did not show decreases in yields even though they had been neither N-fertilized nor inoculated for at least the last 5 years. We hypothesized that the isolated strains maintained high competitiveness and efficiency in fixing adequate N2 levels. A set of strains that showed the highest nodular occupancy in each sampling location were assayed for symbiotic performance under greenhouse and field conditions and comparatively evaluated with Bradyrhizobium japonicum E109, the strain officially recommended for inoculant formulation in Argentina. An inoculant pool, formed by four strains obtained from nodules collected from Cañada Rica, developed higher nodular biomass than B. japonicum E 109 in assays carried out in greenhouses under well irrigated conditions. Additionally, neither nodule production nor specific nitrogenase activity decreased with respect to B. japonicum E 109 when plants were drought stressed during 7 days from sowing. The mean yields obtained under field conditions and plotted against the principal component one (CP1) obtained with an additive main effect and multiplicative interaction (AMMI) model showed that the inoculant pool from Cañada Rica had higher contribution to yield than strain E 109, although with lower environmental stability. The inoculant pool from Cañada Rica could be considered an improved inoculant and be used for preliminary assays, to formulate inoculants in Argentina.
publishDate 2010
dc.date.none.fl_str_mv 2010-09-22
2021-12-17T19:40:15Z
2021-12-17T19:40:15Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10934
https://link.springer.com/article/10.1007/s00374-010-0503-7
0178-2762
1432-0789 (online)
https://doi.org/10.1007/s00374-010-0503-7
url http://hdl.handle.net/20.500.12123/10934
https://link.springer.com/article/10.1007/s00374-010-0503-7
https://doi.org/10.1007/s00374-010-0503-7
identifier_str_mv 0178-2762
1432-0789 (online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Biology and Fertility of Soils 47 : 81–89 (2011)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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