Evaluation of bradyrhizobia strains isolated from field-grown soybean plants in Argentina as improved inoculants
- Autores
- Melchiorre, Mariana; De Luca, Marcos Javier; Gonzalez Anta, Gustavo; Suarez, Paola Alejandra; Lopez, Carlos; Lascano, Hernan Ramiro; Racca, Roberto Walter
- Año de publicación
- 2010
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Bradyrhizobium strains were isolated from nodules obtained from field-grown soybean plants sampled in 12 soybean production locations in Argentina. These fields had been annually cropped with soybean and did not show decreases in yields even though they had been neither N-fertilized nor inoculated for at least the last 5 years. We hypothesized that the isolated strains maintained high competitiveness and efficiency in fixing adequate N2 levels. A set of strains that showed the highest nodular occupancy in each sampling location were assayed for symbiotic performance under greenhouse and field conditions and comparatively evaluated with Bradyrhizobium japonicum E109, the strain officially recommended for inoculant formulation in Argentina. An inoculant pool, formed by four strains obtained from nodules collected from Cañada Rica, developed higher nodular biomass than B. japonicum E 109 in assays carried out in greenhouses under well irrigated conditions. Additionally, neither nodule production nor specific nitrogenase activity decreased with respect to B. japonicum E 109 when plants were drought stressed during 7 days from sowing. The mean yields obtained under field conditions and plotted against the principal component one (CP1) obtained with an additive main effect and multiplicative interaction (AMMI) model showed that the inoculant pool from Cañada Rica had higher contribution to yield than strain E 109, although with lower environmental stability. The inoculant pool from Cañada Rica could be considered an improved inoculant and be used for preliminary assays, to formulate inoculants in Argentina.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Melchiorre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: De Luca, Marcos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Gonzalez Anta, Gustavo. Universidad Nacional del Noroeste de la Provincia de Buenos Aires; Argentina
Fil: Suarez, Paola Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Lopez, Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi. Agencia De Extensión Rural Río Primero; Argentina
Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Racca, Roberto Walter. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina - Fuente
- Biology and Fertility of Soils 47 : 81–89 (2011)
- Materia
-
Bradyrhizobium japonicum
Soja
Argentina
Biological Nitrogen Fixation
Soybeans
Soja
Fijación Biológica del Nitrógeno
Inoculant - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/10934
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Evaluation of bradyrhizobia strains isolated from field-grown soybean plants in Argentina as improved inoculantsMelchiorre, MarianaDe Luca, Marcos JavierGonzalez Anta, GustavoSuarez, Paola AlejandraLopez, CarlosLascano, Hernan RamiroRacca, Roberto WalterBradyrhizobium japonicumSojaArgentinaBiological Nitrogen FixationSoybeansSojaFijación Biológica del NitrógenoInoculantBradyrhizobium strains were isolated from nodules obtained from field-grown soybean plants sampled in 12 soybean production locations in Argentina. These fields had been annually cropped with soybean and did not show decreases in yields even though they had been neither N-fertilized nor inoculated for at least the last 5 years. We hypothesized that the isolated strains maintained high competitiveness and efficiency in fixing adequate N2 levels. A set of strains that showed the highest nodular occupancy in each sampling location were assayed for symbiotic performance under greenhouse and field conditions and comparatively evaluated with Bradyrhizobium japonicum E109, the strain officially recommended for inoculant formulation in Argentina. An inoculant pool, formed by four strains obtained from nodules collected from Cañada Rica, developed higher nodular biomass than B. japonicum E 109 in assays carried out in greenhouses under well irrigated conditions. Additionally, neither nodule production nor specific nitrogenase activity decreased with respect to B. japonicum E 109 when plants were drought stressed during 7 days from sowing. The mean yields obtained under field conditions and plotted against the principal component one (CP1) obtained with an additive main effect and multiplicative interaction (AMMI) model showed that the inoculant pool from Cañada Rica had higher contribution to yield than strain E 109, although with lower environmental stability. The inoculant pool from Cañada Rica could be considered an improved inoculant and be used for preliminary assays, to formulate inoculants in Argentina.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Melchiorre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: De Luca, Marcos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Gonzalez Anta, Gustavo. Universidad Nacional del Noroeste de la Provincia de Buenos Aires; ArgentinaFil: Suarez, Paola Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Lopez, Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi. Agencia De Extensión Rural Río Primero; ArgentinaFil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Racca, Roberto Walter. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaSpringer2021-12-17T19:40:15Z2021-12-17T19:40:15Z2010-09-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10934https://link.springer.com/article/10.1007/s00374-010-0503-70178-27621432-0789 (online)https://doi.org/10.1007/s00374-010-0503-7Biology and Fertility of Soils 47 : 81–89 (2011)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:25Zoai:localhost:20.500.12123/10934instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:26.252INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Evaluation of bradyrhizobia strains isolated from field-grown soybean plants in Argentina as improved inoculants |
title |
Evaluation of bradyrhizobia strains isolated from field-grown soybean plants in Argentina as improved inoculants |
spellingShingle |
Evaluation of bradyrhizobia strains isolated from field-grown soybean plants in Argentina as improved inoculants Melchiorre, Mariana Bradyrhizobium japonicum Soja Argentina Biological Nitrogen Fixation Soybeans Soja Fijación Biológica del Nitrógeno Inoculant |
title_short |
Evaluation of bradyrhizobia strains isolated from field-grown soybean plants in Argentina as improved inoculants |
title_full |
Evaluation of bradyrhizobia strains isolated from field-grown soybean plants in Argentina as improved inoculants |
title_fullStr |
Evaluation of bradyrhizobia strains isolated from field-grown soybean plants in Argentina as improved inoculants |
title_full_unstemmed |
Evaluation of bradyrhizobia strains isolated from field-grown soybean plants in Argentina as improved inoculants |
title_sort |
Evaluation of bradyrhizobia strains isolated from field-grown soybean plants in Argentina as improved inoculants |
dc.creator.none.fl_str_mv |
Melchiorre, Mariana De Luca, Marcos Javier Gonzalez Anta, Gustavo Suarez, Paola Alejandra Lopez, Carlos Lascano, Hernan Ramiro Racca, Roberto Walter |
author |
Melchiorre, Mariana |
author_facet |
Melchiorre, Mariana De Luca, Marcos Javier Gonzalez Anta, Gustavo Suarez, Paola Alejandra Lopez, Carlos Lascano, Hernan Ramiro Racca, Roberto Walter |
author_role |
author |
author2 |
De Luca, Marcos Javier Gonzalez Anta, Gustavo Suarez, Paola Alejandra Lopez, Carlos Lascano, Hernan Ramiro Racca, Roberto Walter |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Bradyrhizobium japonicum Soja Argentina Biological Nitrogen Fixation Soybeans Soja Fijación Biológica del Nitrógeno Inoculant |
topic |
Bradyrhizobium japonicum Soja Argentina Biological Nitrogen Fixation Soybeans Soja Fijación Biológica del Nitrógeno Inoculant |
dc.description.none.fl_txt_mv |
Bradyrhizobium strains were isolated from nodules obtained from field-grown soybean plants sampled in 12 soybean production locations in Argentina. These fields had been annually cropped with soybean and did not show decreases in yields even though they had been neither N-fertilized nor inoculated for at least the last 5 years. We hypothesized that the isolated strains maintained high competitiveness and efficiency in fixing adequate N2 levels. A set of strains that showed the highest nodular occupancy in each sampling location were assayed for symbiotic performance under greenhouse and field conditions and comparatively evaluated with Bradyrhizobium japonicum E109, the strain officially recommended for inoculant formulation in Argentina. An inoculant pool, formed by four strains obtained from nodules collected from Cañada Rica, developed higher nodular biomass than B. japonicum E 109 in assays carried out in greenhouses under well irrigated conditions. Additionally, neither nodule production nor specific nitrogenase activity decreased with respect to B. japonicum E 109 when plants were drought stressed during 7 days from sowing. The mean yields obtained under field conditions and plotted against the principal component one (CP1) obtained with an additive main effect and multiplicative interaction (AMMI) model showed that the inoculant pool from Cañada Rica had higher contribution to yield than strain E 109, although with lower environmental stability. The inoculant pool from Cañada Rica could be considered an improved inoculant and be used for preliminary assays, to formulate inoculants in Argentina. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Melchiorre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: De Luca, Marcos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Gonzalez Anta, Gustavo. Universidad Nacional del Noroeste de la Provincia de Buenos Aires; Argentina Fil: Suarez, Paola Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Lopez, Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi. Agencia De Extensión Rural Río Primero; Argentina Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Racca, Roberto Walter. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina |
description |
Bradyrhizobium strains were isolated from nodules obtained from field-grown soybean plants sampled in 12 soybean production locations in Argentina. These fields had been annually cropped with soybean and did not show decreases in yields even though they had been neither N-fertilized nor inoculated for at least the last 5 years. We hypothesized that the isolated strains maintained high competitiveness and efficiency in fixing adequate N2 levels. A set of strains that showed the highest nodular occupancy in each sampling location were assayed for symbiotic performance under greenhouse and field conditions and comparatively evaluated with Bradyrhizobium japonicum E109, the strain officially recommended for inoculant formulation in Argentina. An inoculant pool, formed by four strains obtained from nodules collected from Cañada Rica, developed higher nodular biomass than B. japonicum E 109 in assays carried out in greenhouses under well irrigated conditions. Additionally, neither nodule production nor specific nitrogenase activity decreased with respect to B. japonicum E 109 when plants were drought stressed during 7 days from sowing. The mean yields obtained under field conditions and plotted against the principal component one (CP1) obtained with an additive main effect and multiplicative interaction (AMMI) model showed that the inoculant pool from Cañada Rica had higher contribution to yield than strain E 109, although with lower environmental stability. The inoculant pool from Cañada Rica could be considered an improved inoculant and be used for preliminary assays, to formulate inoculants in Argentina. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-09-22 2021-12-17T19:40:15Z 2021-12-17T19:40:15Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/10934 https://link.springer.com/article/10.1007/s00374-010-0503-7 0178-2762 1432-0789 (online) https://doi.org/10.1007/s00374-010-0503-7 |
url |
http://hdl.handle.net/20.500.12123/10934 https://link.springer.com/article/10.1007/s00374-010-0503-7 https://doi.org/10.1007/s00374-010-0503-7 |
identifier_str_mv |
0178-2762 1432-0789 (online) |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Biology and Fertility of Soils 47 : 81–89 (2011) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
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INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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