An inoculant Bradyrhizobium strain with increased motility improves yield of soybean crops

Autores
Colla, Delfina; Brignoli, Damián; Iturralde, Esteban Tomás; Covelli, Julieta Mariana; Althabegoiti, María Julia; Perticari, Alejandro; Lodeiro, Aníbal Roberto
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Poster y resumen
Soybean production is very important in Argentina, where more than 20 million hectares are sowed annually with this crop. Since soybean plants possess a very high N-demand, it is crucial that this agricultural activity is developed in a sustainable way because otherwise, this crop could deplete Nnutrition from the soils, leading to erosion, compaction, and flooding. Soybean roots are nodulated by Bradyrhizobium spp., which may fix atmospheric N2 in symbiosis with the plant, thus contributing to keep the N-status of the soil. For this reason and due to their low cost, Bradyrhizobium spp. are widely used in inoculants for soybean crops. However, the efficiency of inoculants is low due to the competition exerted by bradyrhizobia resident in the soil. Among factors that affect the competition for nodulation is the self-propelled motility of the rhizobia. Previously, we developed an artificial selection method to obtain bradyrhizobial strains with higher motility. B. japonicum E109 is the strain recommend by INTA for inoculants production. Therefore, we used E109 to increase its motility, and hereby we obtained the derived B. japonicum E109 m+ strain. This strain possesses 50% more motility than its parental strain in semisolid agar medium, and has the same growth kinetics as the wild type, ruling out the possibility that the increased spreading of E109 m+ in semisolid agar be due to faster growth. B. diazoefficiens have two flagella systems, one subpolar and another lateral, characterized by flagellins of different molecular weights. The subpolar flagellum has constitutive expression and the lateral is inducible with L-arabinose as carbon source, but not with D-mannitol as carbon source. However, E109 m+ expressed both flagella with D-mannitol, as observed with SDS-PAGE of purified flagellins, in agreement with previous results obtained with B. diazoefficiens USDA 110. B. japonicum E109 m+ nodulated soybean and after that, bacteria recovered from nodules maintained the higher motility phenotype. Field trials were performed to estimate yield when the soybean plots were inoculated with E109 m+ or the E109 parental strain. Experiments were carried out in San Antonio de Areco, Province of Buenos Aires, in a soil with a competitive resident Bradyrhizobium spp. population, employing a randomized complete block design that included uninoculated controls. Grain yields were compared by ANOVA, which indicated that inoculation with E109 m+ led to significantly higher yield than inoculation with E109 wild type. Our results suggested that inoculation of soybean with improved motility strains could increase soybean yield by enhancing competition for nodulation in a sustainable way.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Colla, Delfina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Brignoli, Damián.Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Iturralde, Esteban Tomás.Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Covelli, Julieta Mariana. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Bioquímica, Microbiología e Interacciones Biológicas en el Suelo; Argentina
Fil: Althabegoiti, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Perticari, Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Lodeiro, Aníbal Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Lodeiro, Aníbal Roberto. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Laboratorio de Genética; Argentina
Fuente
V Congreso Argentino de Microbiología Agrícola y Ambiental (Virtual), 15 al 17 septiembre de 2021
Materia
Bradyrhizobium
Bradyrhizobium japonicum
Inoculación
Soja
Crops
Inoculation
Soybeans
Cultivos
Inoculantes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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spelling An inoculant Bradyrhizobium strain with increased motility improves yield of soybean cropsColla, DelfinaBrignoli, DamiánIturralde, Esteban TomásCovelli, Julieta MarianaAlthabegoiti, María JuliaPerticari, AlejandroLodeiro, Aníbal RobertoBradyrhizobiumBradyrhizobium japonicumInoculaciónSojaCropsInoculationSoybeansCultivosInoculantesPoster y resumenSoybean production is very important in Argentina, where more than 20 million hectares are sowed annually with this crop. Since soybean plants possess a very high N-demand, it is crucial that this agricultural activity is developed in a sustainable way because otherwise, this crop could deplete Nnutrition from the soils, leading to erosion, compaction, and flooding. Soybean roots are nodulated by Bradyrhizobium spp., which may fix atmospheric N2 in symbiosis with the plant, thus contributing to keep the N-status of the soil. For this reason and due to their low cost, Bradyrhizobium spp. are widely used in inoculants for soybean crops. However, the efficiency of inoculants is low due to the competition exerted by bradyrhizobia resident in the soil. Among factors that affect the competition for nodulation is the self-propelled motility of the rhizobia. Previously, we developed an artificial selection method to obtain bradyrhizobial strains with higher motility. B. japonicum E109 is the strain recommend by INTA for inoculants production. Therefore, we used E109 to increase its motility, and hereby we obtained the derived B. japonicum E109 m+ strain. This strain possesses 50% more motility than its parental strain in semisolid agar medium, and has the same growth kinetics as the wild type, ruling out the possibility that the increased spreading of E109 m+ in semisolid agar be due to faster growth. B. diazoefficiens have two flagella systems, one subpolar and another lateral, characterized by flagellins of different molecular weights. The subpolar flagellum has constitutive expression and the lateral is inducible with L-arabinose as carbon source, but not with D-mannitol as carbon source. However, E109 m+ expressed both flagella with D-mannitol, as observed with SDS-PAGE of purified flagellins, in agreement with previous results obtained with B. diazoefficiens USDA 110. B. japonicum E109 m+ nodulated soybean and after that, bacteria recovered from nodules maintained the higher motility phenotype. Field trials were performed to estimate yield when the soybean plots were inoculated with E109 m+ or the E109 parental strain. Experiments were carried out in San Antonio de Areco, Province of Buenos Aires, in a soil with a competitive resident Bradyrhizobium spp. population, employing a randomized complete block design that included uninoculated controls. Grain yields were compared by ANOVA, which indicated that inoculation with E109 m+ led to significantly higher yield than inoculation with E109 wild type. Our results suggested that inoculation of soybean with improved motility strains could increase soybean yield by enhancing competition for nodulation in a sustainable way.Instituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Colla, Delfina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Brignoli, Damián.Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Iturralde, Esteban Tomás.Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Covelli, Julieta Mariana. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Bioquímica, Microbiología e Interacciones Biológicas en el Suelo; ArgentinaFil: Althabegoiti, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Perticari, Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Lodeiro, Aníbal Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Lodeiro, Aníbal Roberto. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Laboratorio de Genética; ArgentinaAsociación Argentina de Microbiología2023-02-14T10:34:14Z2023-02-14T10:34:14Z2021-09-15info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfhttp://hdl.handle.net/20.500.12123/13960V Congreso Argentino de Microbiología Agrícola y Ambiental (Virtual), 15 al 17 septiembre de 2021reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:49:43Zoai:localhost:20.500.12123/13960instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:44.269INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv An inoculant Bradyrhizobium strain with increased motility improves yield of soybean crops
title An inoculant Bradyrhizobium strain with increased motility improves yield of soybean crops
spellingShingle An inoculant Bradyrhizobium strain with increased motility improves yield of soybean crops
Colla, Delfina
Bradyrhizobium
Bradyrhizobium japonicum
Inoculación
Soja
Crops
Inoculation
Soybeans
Cultivos
Inoculantes
title_short An inoculant Bradyrhizobium strain with increased motility improves yield of soybean crops
title_full An inoculant Bradyrhizobium strain with increased motility improves yield of soybean crops
title_fullStr An inoculant Bradyrhizobium strain with increased motility improves yield of soybean crops
title_full_unstemmed An inoculant Bradyrhizobium strain with increased motility improves yield of soybean crops
title_sort An inoculant Bradyrhizobium strain with increased motility improves yield of soybean crops
dc.creator.none.fl_str_mv Colla, Delfina
Brignoli, Damián
Iturralde, Esteban Tomás
Covelli, Julieta Mariana
Althabegoiti, María Julia
Perticari, Alejandro
Lodeiro, Aníbal Roberto
author Colla, Delfina
author_facet Colla, Delfina
Brignoli, Damián
Iturralde, Esteban Tomás
Covelli, Julieta Mariana
Althabegoiti, María Julia
Perticari, Alejandro
Lodeiro, Aníbal Roberto
author_role author
author2 Brignoli, Damián
Iturralde, Esteban Tomás
Covelli, Julieta Mariana
Althabegoiti, María Julia
Perticari, Alejandro
Lodeiro, Aníbal Roberto
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Bradyrhizobium
Bradyrhizobium japonicum
Inoculación
Soja
Crops
Inoculation
Soybeans
Cultivos
Inoculantes
topic Bradyrhizobium
Bradyrhizobium japonicum
Inoculación
Soja
Crops
Inoculation
Soybeans
Cultivos
Inoculantes
dc.description.none.fl_txt_mv Poster y resumen
Soybean production is very important in Argentina, where more than 20 million hectares are sowed annually with this crop. Since soybean plants possess a very high N-demand, it is crucial that this agricultural activity is developed in a sustainable way because otherwise, this crop could deplete Nnutrition from the soils, leading to erosion, compaction, and flooding. Soybean roots are nodulated by Bradyrhizobium spp., which may fix atmospheric N2 in symbiosis with the plant, thus contributing to keep the N-status of the soil. For this reason and due to their low cost, Bradyrhizobium spp. are widely used in inoculants for soybean crops. However, the efficiency of inoculants is low due to the competition exerted by bradyrhizobia resident in the soil. Among factors that affect the competition for nodulation is the self-propelled motility of the rhizobia. Previously, we developed an artificial selection method to obtain bradyrhizobial strains with higher motility. B. japonicum E109 is the strain recommend by INTA for inoculants production. Therefore, we used E109 to increase its motility, and hereby we obtained the derived B. japonicum E109 m+ strain. This strain possesses 50% more motility than its parental strain in semisolid agar medium, and has the same growth kinetics as the wild type, ruling out the possibility that the increased spreading of E109 m+ in semisolid agar be due to faster growth. B. diazoefficiens have two flagella systems, one subpolar and another lateral, characterized by flagellins of different molecular weights. The subpolar flagellum has constitutive expression and the lateral is inducible with L-arabinose as carbon source, but not with D-mannitol as carbon source. However, E109 m+ expressed both flagella with D-mannitol, as observed with SDS-PAGE of purified flagellins, in agreement with previous results obtained with B. diazoefficiens USDA 110. B. japonicum E109 m+ nodulated soybean and after that, bacteria recovered from nodules maintained the higher motility phenotype. Field trials were performed to estimate yield when the soybean plots were inoculated with E109 m+ or the E109 parental strain. Experiments were carried out in San Antonio de Areco, Province of Buenos Aires, in a soil with a competitive resident Bradyrhizobium spp. population, employing a randomized complete block design that included uninoculated controls. Grain yields were compared by ANOVA, which indicated that inoculation with E109 m+ led to significantly higher yield than inoculation with E109 wild type. Our results suggested that inoculation of soybean with improved motility strains could increase soybean yield by enhancing competition for nodulation in a sustainable way.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Colla, Delfina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Brignoli, Damián.Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Iturralde, Esteban Tomás.Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Covelli, Julieta Mariana. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Bioquímica, Microbiología e Interacciones Biológicas en el Suelo; Argentina
Fil: Althabegoiti, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Perticari, Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Lodeiro, Aníbal Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Lodeiro, Aníbal Roberto. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Laboratorio de Genética; Argentina
description Poster y resumen
publishDate 2021
dc.date.none.fl_str_mv 2021-09-15
2023-02-14T10:34:14Z
2023-02-14T10:34:14Z
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/publishedVersion
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dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/13960
url http://hdl.handle.net/20.500.12123/13960
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Asociación Argentina de Microbiología
publisher.none.fl_str_mv Asociación Argentina de Microbiología
dc.source.none.fl_str_mv V Congreso Argentino de Microbiología Agrícola y Ambiental (Virtual), 15 al 17 septiembre de 2021
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
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