Soybean - nodulating strains with low intrinsic competitiveness for nodulation, good symbiotic performance, and stress - tolerance isolated from soybean - cropped soils in Argentin...

• Autores: Iturralde, Esteban T.; Covelli, Julieta M.; Alvarez, Florencia; Pérez Giménez, Julieta; Arrese Igor, Cesar; Lodeiro, Aníbal R.
• Año de publicación: 2019
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Frontiers in Microbiology; Vol.10; Article 1061; https://www.frontiersin.org/
• Materia: BRADYRHIZOBIUM; ALLOCHTHONOUS POPULATION; N2 - FIXATION; INOCULANT; NODULATION
• Institución: Universidad de Buenos Aires. Facultad de Agronomía

Fil: Iturralde, Esteban T. Universidad Nacional de La Plata (UNLP). Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular (IBBM). La Plata, Buenos Aires, Argentina.
Fil: Iturralde, Esteban T. CONICET. CCT. La Plata, Buenos Aires, Argentina.
Fil: Covelli, Julieta M. Universidad Nacional de La Plata (UNLP). Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular (IBBM). La Plata, Buenos Aires, Argentina.
Fil: Covelli, Julieta M. CONICET. CCT. La Plata, Buenos Aires, Argentina.
Fil: Covelli, Julieta M. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Bernal, Buenos Aires, Argentina.
Fil: Alvarez, Florencia. Universidad Nacional de La Plata (UNLP). Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular (IBBM). La Plata, Buenos Aires, Argentina.
Fil: Alvarez, Florencia. CONICET. CCT. La Plata, Buenos Aires, Argentina.
Fil: Alvarez, Florencia. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Fil: Alvarez, Florencia. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Fil: Pérez Giménez, Julieta. Universidad Nacional de La Plata (UNLP). Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular (IBBM). La Plata, Buenos Aires, Argentina.
Fil: Pérez Giménez, Julieta. CONICET. CCT. La Plata, Buenos Aires, Argentina.
Fil: Arrese Igor, Cesar. Universidad Pública de Navarra. Institute for Multidisciplinary Research in Applied Biology (IMAB). Pamplona, Spain.
Fil: Lodeiro, Aníbal R. Universidad Nacional de La Plata (UNLP). Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular (IBBM). La Plata, Buenos Aires, Argentina.
Fil: Lodeiro, Aníbal R. CONICET. CCT. La Plata, Buenos Aires, Argentina.
Soybean is the most important oilseed in the world, cropped in 120–130 million hectares each year. The three most important soybean producers are Argentina, Brazil, and United States, where soybean crops are routinely inoculated with symbiotic N2-fixing Bradyrhizobium spp. This extended inoculation gave rise to soybeannodulating allochthonous populations (SNAPs) that compete against new inoculant for nodulation, thus impairing yield responses. Competitiveness depends on intrinsic factors contributed by genotype, extrinsic ones determined by growth and environmental conditions, and strain persistence in the soil. To assess these factors in Argentinean SNAPs, we studied 58 isolates from five sites of the main soybean cropping area. BOX-A1R DNA fingerprint distributed these isolates in 10 clades that paralleled the pHs of their original soils. By contrast, reference Bradyrhizobium spp. strains, including those used as soybean-inoculants, were confined to a single clade. More detailed characterization of a subset of 11 SNAP-isolates revealed that five were Bradyrhizobium japonicum, two Bradyrhizobium elkanii, two Rhizobium radiobacter (formerly Agrobacterium tumefaciens), one Bradyrhizobium diazoefficiens, and one Paenibacillus glycanilyticus which did not nodulate when inoculated alone, and therefore was excluded from further characterization. The remaining subset of 10 SNAPisolates was used for deeper characterization. All SNAP-isolates were luminum- and heat-tolerant, and most of them were glyphosate-tolerant. Meanwhile, inoculant strains tested were sensitive to aluminum and glyphosate. In addition, all SNAP-isolates were motile to different degrees. Only three SNAP-isolates were deficient for N2-fixation, and none was intrinsically more competitive than the inoculant strain. These results are in contrast to the general belief that rhizobia from soil populations evolved as intrinsically more competitive for nodulation and less N2-fixing effective than inoculants strains. Shoot:root ratios, both as dry biomass and as total N, were highly correlated with leaf ureide contents, and therefore may be easy indicators of N2-fixing performance, suggesting that highly effective N2-fixing and well-adapted strains may be readily selected from SNAPs. In addition, intrinsic competitiveness of the inoculants strains seems already optimized against SNAP strains, and therefore our efforts to improve nodules occupation by inoculated strains should focus on the optimization of extrinsic competitiveness factors, such as inoculant formulation and inoculation technology.
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