Papaya ringspot virus W infecting Luffa aegyptiaca in Cuba

Autores
Cabrera Mederos, Dariel; Giolitti, Fabian; Nome Docampo, Claudia; Bejerman, Nicolas Esteban; Portal, Orelvis
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Luffa aegyptiaca (sponge gourd) plants showing severe leaf mosaic and deformation symptoms were observed in Villa Clara, Cuba. Electron microscopy observations of leaf dip preparations revealed flexuous filamentous particles, which were identified as Papaya ringspot virus (PRSV) by ELISA test. PRSV was mechanically transmitted to healthy Cucurbita moschata plants. Sequence analysis of the RT-PCR product obtained from the capsid protein gene showed highest identity with other PRSV isolates from United States of America, Australia, Venezuela, Brazil, India and Cuba, ranging between 93.2–96.5% and 93.4–98.1% for nucleotide and amino acid sequences, respectively. Phylogenetic analysis showed two main clusters. Cluster I included isolates from the Americas-Australia group and India, including sponge gourd isolate, while the cluster II included isolates from China, Thailand and Taiwan. To our knowledge, this is the first report of PRSV-W infecting sponge gourd in Cuba.
Instituto de Patología Vegetal
Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Central “Marta Abreu” de Las Villas. Facultad de Ciencias Agropecuarias. Departamento de Agronomía; Cuba
Fil. Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Portal, Orelvis. Universidad Central “Marta Abreu” de Las Villas. Facultad de Ciencias Agropecuarias. Departamento de Biología; Cuba
Fuente
Australasian plant disease notes 12 : 5. (December 2017)
Materia
Papayas
Virus de las Plantas
Plant Viruses
Luffa Aegyptica
Papaya Ringspot Virus
Virus de la Mancha de Anillo de la Papaya
Sponge Gourd
Cuba
Sequencing
Secuenciación
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3647
Acceder
id INTADig_c56e17ed9a332939d99bbbfe9a134fb3
oai_identifier_str oai:localhost:20.500.12123/3647
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Papaya ringspot virus W infecting Luffa aegyptiaca in CubaCabrera Mederos, DarielGiolitti, FabianNome Docampo, ClaudiaBejerman, Nicolas EstebanPortal, OrelvisPapayasVirus de las PlantasPlant VirusesLuffa AegypticaPapaya Ringspot VirusVirus de la Mancha de Anillo de la PapayaSponge GourdCubaSequencingSecuenciaciónLuffa aegyptiaca (sponge gourd) plants showing severe leaf mosaic and deformation symptoms were observed in Villa Clara, Cuba. Electron microscopy observations of leaf dip preparations revealed flexuous filamentous particles, which were identified as Papaya ringspot virus (PRSV) by ELISA test. PRSV was mechanically transmitted to healthy Cucurbita moschata plants. Sequence analysis of the RT-PCR product obtained from the capsid protein gene showed highest identity with other PRSV isolates from United States of America, Australia, Venezuela, Brazil, India and Cuba, ranging between 93.2–96.5% and 93.4–98.1% for nucleotide and amino acid sequences, respectively. Phylogenetic analysis showed two main clusters. Cluster I included isolates from the Americas-Australia group and India, including sponge gourd isolate, while the cluster II included isolates from China, Thailand and Taiwan. To our knowledge, this is the first report of PRSV-W infecting sponge gourd in Cuba.Instituto de Patología VegetalFil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Central “Marta Abreu” de Las Villas. Facultad de Ciencias Agropecuarias. Departamento de Agronomía; CubaFil. Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Portal, Orelvis. Universidad Central “Marta Abreu” de Las Villas. Facultad de Ciencias Agropecuarias. Departamento de Biología; CubaSpringer2018-10-19T14:57:42Z2018-10-19T14:57:42Z2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/3647https://link.springer.com/article/10.1007%2Fs13314-017-0229-6#citeas1833-928X (Online)https://doi.org/10.1007/s13314-017-0229-6Australasian plant disease notes 12 : 5. (December 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengCuba (nation)info:eu-repo/semantics/restrictedAccess2025-09-11T10:22:47Zoai:localhost:20.500.12123/3647instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-11 10:22:48.225INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Papaya ringspot virus W infecting Luffa aegyptiaca in Cuba
title Papaya ringspot virus W infecting Luffa aegyptiaca in Cuba
spellingShingle Papaya ringspot virus W infecting Luffa aegyptiaca in Cuba
Cabrera Mederos, Dariel
Papayas
Virus de las Plantas
Plant Viruses
Luffa Aegyptica
Papaya Ringspot Virus
Virus de la Mancha de Anillo de la Papaya
Sponge Gourd
Cuba
Sequencing
Secuenciación
title_short Papaya ringspot virus W infecting Luffa aegyptiaca in Cuba
title_full Papaya ringspot virus W infecting Luffa aegyptiaca in Cuba
title_fullStr Papaya ringspot virus W infecting Luffa aegyptiaca in Cuba
title_full_unstemmed Papaya ringspot virus W infecting Luffa aegyptiaca in Cuba
title_sort Papaya ringspot virus W infecting Luffa aegyptiaca in Cuba
dc.creator.none.fl_str_mv Cabrera Mederos, Dariel
Giolitti, Fabian
Nome Docampo, Claudia
Bejerman, Nicolas Esteban
Portal, Orelvis
author Cabrera Mederos, Dariel
author_facet Cabrera Mederos, Dariel
Giolitti, Fabian
Nome Docampo, Claudia
Bejerman, Nicolas Esteban
Portal, Orelvis
author_role author
author2 Giolitti, Fabian
Nome Docampo, Claudia
Bejerman, Nicolas Esteban
Portal, Orelvis
author2_role author
author
author
author
dc.subject.none.fl_str_mv Papayas
Virus de las Plantas
Plant Viruses
Luffa Aegyptica
Papaya Ringspot Virus
Virus de la Mancha de Anillo de la Papaya
Sponge Gourd
Cuba
Sequencing
Secuenciación
topic Papayas
Virus de las Plantas
Plant Viruses
Luffa Aegyptica
Papaya Ringspot Virus
Virus de la Mancha de Anillo de la Papaya
Sponge Gourd
Cuba
Sequencing
Secuenciación
dc.description.none.fl_txt_mv Luffa aegyptiaca (sponge gourd) plants showing severe leaf mosaic and deformation symptoms were observed in Villa Clara, Cuba. Electron microscopy observations of leaf dip preparations revealed flexuous filamentous particles, which were identified as Papaya ringspot virus (PRSV) by ELISA test. PRSV was mechanically transmitted to healthy Cucurbita moschata plants. Sequence analysis of the RT-PCR product obtained from the capsid protein gene showed highest identity with other PRSV isolates from United States of America, Australia, Venezuela, Brazil, India and Cuba, ranging between 93.2–96.5% and 93.4–98.1% for nucleotide and amino acid sequences, respectively. Phylogenetic analysis showed two main clusters. Cluster I included isolates from the Americas-Australia group and India, including sponge gourd isolate, while the cluster II included isolates from China, Thailand and Taiwan. To our knowledge, this is the first report of PRSV-W infecting sponge gourd in Cuba.
Instituto de Patología Vegetal
Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Central “Marta Abreu” de Las Villas. Facultad de Ciencias Agropecuarias. Departamento de Agronomía; Cuba
Fil. Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Portal, Orelvis. Universidad Central “Marta Abreu” de Las Villas. Facultad de Ciencias Agropecuarias. Departamento de Biología; Cuba
description Luffa aegyptiaca (sponge gourd) plants showing severe leaf mosaic and deformation symptoms were observed in Villa Clara, Cuba. Electron microscopy observations of leaf dip preparations revealed flexuous filamentous particles, which were identified as Papaya ringspot virus (PRSV) by ELISA test. PRSV was mechanically transmitted to healthy Cucurbita moschata plants. Sequence analysis of the RT-PCR product obtained from the capsid protein gene showed highest identity with other PRSV isolates from United States of America, Australia, Venezuela, Brazil, India and Cuba, ranging between 93.2–96.5% and 93.4–98.1% for nucleotide and amino acid sequences, respectively. Phylogenetic analysis showed two main clusters. Cluster I included isolates from the Americas-Australia group and India, including sponge gourd isolate, while the cluster II included isolates from China, Thailand and Taiwan. To our knowledge, this is the first report of PRSV-W infecting sponge gourd in Cuba.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
2018-10-19T14:57:42Z
2018-10-19T14:57:42Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/3647
https://link.springer.com/article/10.1007%2Fs13314-017-0229-6#citeas
1833-928X (Online)
https://doi.org/10.1007/s13314-017-0229-6
url http://hdl.handle.net/20.500.12123/3647
https://link.springer.com/article/10.1007%2Fs13314-017-0229-6#citeas
https://doi.org/10.1007/s13314-017-0229-6
identifier_str_mv 1833-928X (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Cuba (nation)
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Australasian plant disease notes 12 : 5. (December 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1842975473117691904
score 12.993085

Publicaciones similares