First report of Papaya ringspot virus infecting Carica papaya in Argentina

Autores
Cabrera Mederos, Dariel; Dal Zotto, Angelica; Galdeano, E.; Portal, O.; Giolitti, Fabian
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Papaya plants (Carica papaya L.) with severe leaf mosaic and deformation symptoms were observed and collected in the Formosa, Corrientes and Misiones provinces of northern Argentina. These symptoms were similar to those induced by Papaya ringspot virus (PRSV-P) (Tripathi et al., 2008). Electron microscopy observations of leaf dip preparations from symptomatic papaya leaves revealed typical flexuous potyvirus particles of ca. 800×12 nm (Francki et al., 1985). Papaya leaf extracts from affected plants were mechanically inoculated on healthy papaya plants, which showed typical symptoms of the disease. The presence of the virus in symptomatic plants was tested and confirmed by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), using a specific PRSV antiserum (Agdia, Elkhart, IN). Total RNA was extracted using RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and tested by reverse transcription-polymerase chain reaction (RT-PCR) with specific PRSV coat protein gene primers: BoCP_Fwd 5’-TCCAAGAATGAAGCTGTGGACGCTGGTT-3’ and BoCP_Rev 5’-TYAGTTGCGCATACCCAGGAGAGAGT-3’. The RT-PCR amplicons of the expected size were purified and directly sequenced in both directions at Macrogen Inc. (Seoul, Korea). BLASTn analysis of the sequenced fragments (828 bp) (GenBank accession Nos. KX385113 to KX385116) showed 97 to 98% nucleotide sequence identity with previously reported PRSV-P isolates from Brazil (AF344642, JQ755427, JQ755424). To the best of our knowledge, this is the first report of the occurrence of PRSV in papaya plants in Argentina. Due to the devastating effects of PRSV in papaya (Tripathi et al., 2008) and the recent increase of cultivation area in northern Argentina, it becomes necessary to implement management strategies to control this disease and avoid the introduction of the virus into new production areas.
Instituto de Patología Vegetal
Fil: Cabrera Mederos, Dariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Dal Zotto, Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Galdeano, Ernestina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina
Fil: Galdeano, Ernestina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina
Fil: Portal, O. Universidad Central “Marta Abreu” de Las Villas. Facultad de Ciencias Agropecuarias; Cuba
Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fuente
Journal of Plant Pathology 98 (3) : 687 (2016)
Materia
Carica Papaya
Argentina
Carica Papaya
Potyvirus
Virus
Viruses
Papaya Ringspot Virus
Papayas
Papaya
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/9708
Acceder
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oai_identifier_str oai:localhost:20.500.12123/9708
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network_name_str INTA Digital (INTA)
spelling First report of Papaya ringspot virus infecting Carica papaya in ArgentinaCabrera Mederos, DarielDal Zotto, AngelicaGaldeano, E.Portal, O.Giolitti, FabianCarica PapayaArgentinaCarica PapayaPotyvirusVirusVirusesPapaya Ringspot VirusPapayasPapayaPapaya plants (Carica papaya L.) with severe leaf mosaic and deformation symptoms were observed and collected in the Formosa, Corrientes and Misiones provinces of northern Argentina. These symptoms were similar to those induced by Papaya ringspot virus (PRSV-P) (Tripathi et al., 2008). Electron microscopy observations of leaf dip preparations from symptomatic papaya leaves revealed typical flexuous potyvirus particles of ca. 800×12 nm (Francki et al., 1985). Papaya leaf extracts from affected plants were mechanically inoculated on healthy papaya plants, which showed typical symptoms of the disease. The presence of the virus in symptomatic plants was tested and confirmed by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), using a specific PRSV antiserum (Agdia, Elkhart, IN). Total RNA was extracted using RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and tested by reverse transcription-polymerase chain reaction (RT-PCR) with specific PRSV coat protein gene primers: BoCP_Fwd 5’-TCCAAGAATGAAGCTGTGGACGCTGGTT-3’ and BoCP_Rev 5’-TYAGTTGCGCATACCCAGGAGAGAGT-3’. The RT-PCR amplicons of the expected size were purified and directly sequenced in both directions at Macrogen Inc. (Seoul, Korea). BLASTn analysis of the sequenced fragments (828 bp) (GenBank accession Nos. KX385113 to KX385116) showed 97 to 98% nucleotide sequence identity with previously reported PRSV-P isolates from Brazil (AF344642, JQ755427, JQ755424). To the best of our knowledge, this is the first report of the occurrence of PRSV in papaya plants in Argentina. Due to the devastating effects of PRSV in papaya (Tripathi et al., 2008) and the recent increase of cultivation area in northern Argentina, it becomes necessary to implement management strategies to control this disease and avoid the introduction of the virus into new production areas.Instituto de Patología VegetalFil: Cabrera Mederos, Dariel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Dal Zotto, Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Galdeano, Ernestina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; ArgentinaFil: Galdeano, Ernestina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; ArgentinaFil: Portal, O. Universidad Central “Marta Abreu” de Las Villas. Facultad de Ciencias Agropecuarias; CubaFil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaDipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università di Bari2021-07-01T12:16:17Z2021-07-01T12:16:17Z2016-06-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9708http://www.sipav.org/main/jpp/index.php/jpp/article/view/36911125-46532239-7264 (online)http://dx.doi.org/10.4454/JPP.V98I3.017Journal of Plant Pathology 98 (3) : 687 (2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNPV-1135022/AR./Identificación y desarrollo de protocolos para la detección de patógenos de importancia agrícola.info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:15Zoai:localhost:20.500.12123/9708instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:16.076INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv First report of Papaya ringspot virus infecting Carica papaya in Argentina
title First report of Papaya ringspot virus infecting Carica papaya in Argentina
spellingShingle First report of Papaya ringspot virus infecting Carica papaya in Argentina
Cabrera Mederos, Dariel
Carica Papaya
Argentina
Carica Papaya
Potyvirus
Virus
Viruses
Papaya Ringspot Virus
Papayas
Papaya
title_short First report of Papaya ringspot virus infecting Carica papaya in Argentina
title_full First report of Papaya ringspot virus infecting Carica papaya in Argentina
title_fullStr First report of Papaya ringspot virus infecting Carica papaya in Argentina
title_full_unstemmed First report of Papaya ringspot virus infecting Carica papaya in Argentina
title_sort First report of Papaya ringspot virus infecting Carica papaya in Argentina
dc.creator.none.fl_str_mv Cabrera Mederos, Dariel
Dal Zotto, Angelica
Galdeano, E.
Portal, O.
Giolitti, Fabian
author Cabrera Mederos, Dariel
author_facet Cabrera Mederos, Dariel
Dal Zotto, Angelica
Galdeano, E.
Portal, O.
Giolitti, Fabian
author_role author
author2 Dal Zotto, Angelica
Galdeano, E.
Portal, O.
Giolitti, Fabian
author2_role author
author
author
author
dc.subject.none.fl_str_mv Carica Papaya
Argentina
Carica Papaya
Potyvirus
Virus
Viruses
Papaya Ringspot Virus
Papayas
Papaya
topic Carica Papaya
Argentina
Carica Papaya
Potyvirus
Virus
Viruses
Papaya Ringspot Virus
Papayas
Papaya
dc.description.none.fl_txt_mv Papaya plants (Carica papaya L.) with severe leaf mosaic and deformation symptoms were observed and collected in the Formosa, Corrientes and Misiones provinces of northern Argentina. These symptoms were similar to those induced by Papaya ringspot virus (PRSV-P) (Tripathi et al., 2008). Electron microscopy observations of leaf dip preparations from symptomatic papaya leaves revealed typical flexuous potyvirus particles of ca. 800×12 nm (Francki et al., 1985). Papaya leaf extracts from affected plants were mechanically inoculated on healthy papaya plants, which showed typical symptoms of the disease. The presence of the virus in symptomatic plants was tested and confirmed by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), using a specific PRSV antiserum (Agdia, Elkhart, IN). Total RNA was extracted using RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and tested by reverse transcription-polymerase chain reaction (RT-PCR) with specific PRSV coat protein gene primers: BoCP_Fwd 5’-TCCAAGAATGAAGCTGTGGACGCTGGTT-3’ and BoCP_Rev 5’-TYAGTTGCGCATACCCAGGAGAGAGT-3’. The RT-PCR amplicons of the expected size were purified and directly sequenced in both directions at Macrogen Inc. (Seoul, Korea). BLASTn analysis of the sequenced fragments (828 bp) (GenBank accession Nos. KX385113 to KX385116) showed 97 to 98% nucleotide sequence identity with previously reported PRSV-P isolates from Brazil (AF344642, JQ755427, JQ755424). To the best of our knowledge, this is the first report of the occurrence of PRSV in papaya plants in Argentina. Due to the devastating effects of PRSV in papaya (Tripathi et al., 2008) and the recent increase of cultivation area in northern Argentina, it becomes necessary to implement management strategies to control this disease and avoid the introduction of the virus into new production areas.
Instituto de Patología Vegetal
Fil: Cabrera Mederos, Dariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Dal Zotto, Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Galdeano, Ernestina. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina
Fil: Galdeano, Ernestina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina
Fil: Portal, O. Universidad Central “Marta Abreu” de Las Villas. Facultad de Ciencias Agropecuarias; Cuba
Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
description Papaya plants (Carica papaya L.) with severe leaf mosaic and deformation symptoms were observed and collected in the Formosa, Corrientes and Misiones provinces of northern Argentina. These symptoms were similar to those induced by Papaya ringspot virus (PRSV-P) (Tripathi et al., 2008). Electron microscopy observations of leaf dip preparations from symptomatic papaya leaves revealed typical flexuous potyvirus particles of ca. 800×12 nm (Francki et al., 1985). Papaya leaf extracts from affected plants were mechanically inoculated on healthy papaya plants, which showed typical symptoms of the disease. The presence of the virus in symptomatic plants was tested and confirmed by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), using a specific PRSV antiserum (Agdia, Elkhart, IN). Total RNA was extracted using RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and tested by reverse transcription-polymerase chain reaction (RT-PCR) with specific PRSV coat protein gene primers: BoCP_Fwd 5’-TCCAAGAATGAAGCTGTGGACGCTGGTT-3’ and BoCP_Rev 5’-TYAGTTGCGCATACCCAGGAGAGAGT-3’. The RT-PCR amplicons of the expected size were purified and directly sequenced in both directions at Macrogen Inc. (Seoul, Korea). BLASTn analysis of the sequenced fragments (828 bp) (GenBank accession Nos. KX385113 to KX385116) showed 97 to 98% nucleotide sequence identity with previously reported PRSV-P isolates from Brazil (AF344642, JQ755427, JQ755424). To the best of our knowledge, this is the first report of the occurrence of PRSV in papaya plants in Argentina. Due to the devastating effects of PRSV in papaya (Tripathi et al., 2008) and the recent increase of cultivation area in northern Argentina, it becomes necessary to implement management strategies to control this disease and avoid the introduction of the virus into new production areas.
publishDate 2016
dc.date.none.fl_str_mv 2016-06-20
2021-07-01T12:16:17Z
2021-07-01T12:16:17Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/9708
http://www.sipav.org/main/jpp/index.php/jpp/article/view/3691
1125-4653
2239-7264 (online)
http://dx.doi.org/10.4454/JPP.V98I3.017
url http://hdl.handle.net/20.500.12123/9708
http://www.sipav.org/main/jpp/index.php/jpp/article/view/3691
http://dx.doi.org/10.4454/JPP.V98I3.017
identifier_str_mv 1125-4653
2239-7264 (online)
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNPV-1135022/AR./Identificación y desarrollo de protocolos para la detección de patógenos de importancia agrícola.
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università di Bari
publisher.none.fl_str_mv Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università di Bari
dc.source.none.fl_str_mv Journal of Plant Pathology 98 (3) : 687 (2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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