First report of Papaya ringspot virus infecting Carica papaya in Argentina

Autores
Cabrera Mederos, Dariel; Dal Zotto, Angélica; Portal, Orelvis; Giolitti, Fabián José
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Papaya plants (Carica papaya L.) with severe leaf mosaic and deformation symptoms were observed and collected in the Formosa, Corrientes and Misiones provinces of northern Argentina. These symptoms were similar to those induced by Papaya ringspot virus (PRSV-P) (Tripathi et al., 2008). Electron microscopy observations of leaf dip preparations from symptomatic papaya leaves revealed typical flexuous potyvirus particles of ca. 800×12 nm (Francki et al., 1985). Papaya leaf extracts from affected plants were mechanically inoculated on healthy papaya plants, which showed typical symptoms of the disease. The presence of the virus in symptomatic plants was tested and confirmed by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), using a specific PRSV antiserum (Agdia, Elkhart, IN). Total RNA was extracted using RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and tested by reverse transcription-polymerase chain reaction (RT-PCR) with specific PRSV coat protein gene primers: BoCP_Fwd 5?-TCCAAGAATGAAGCTGTGGACGCTGGTT-3? and BoCP_Rev 5?-TYAGTTGCGCATACCCAGGAGAGAGT-3?. The RT-PCR amplicons of the expected size were purified and directly sequenced in both directions at Macrogen Inc. (Seoul, Korea). BLASTn analysis of the sequenced fragments (828 bp) (GenBank accession Nos. KX385113 to KX385116) showed 97 to 98% nucleotide sequence identity with previously reported PRSV-P isolates from Brazil (AF344642, JQ755427, JQ755424). To the best of our knowledge, this is the first report of the occurrence of PRSV in papaya plants in Argentina. Due to the devastating effects of PRSV in papaya (Tripathi et al., 2008) and the recent increase of cultivation area in northern Argentina, it becomes necessary to implement management strategies to control this disease and avoid the introduction of the virus into new production areas.
Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dal Zotto, Angélica. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Portal, Orelvis. Universidad Central ´´marta Abreu´´ de Las Villas; Cuba
Fil: Giolitti, Fabián José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Materia
Papaya ringspot virus
Carica papaya
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/180082
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/180082
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling First report of Papaya ringspot virus infecting Carica papaya in ArgentinaCabrera Mederos, DarielDal Zotto, AngélicaPortal, OrelvisGiolitti, Fabián JoséPapaya ringspot virusCarica papayahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Papaya plants (Carica papaya L.) with severe leaf mosaic and deformation symptoms were observed and collected in the Formosa, Corrientes and Misiones provinces of northern Argentina. These symptoms were similar to those induced by Papaya ringspot virus (PRSV-P) (Tripathi et al., 2008). Electron microscopy observations of leaf dip preparations from symptomatic papaya leaves revealed typical flexuous potyvirus particles of ca. 800×12 nm (Francki et al., 1985). Papaya leaf extracts from affected plants were mechanically inoculated on healthy papaya plants, which showed typical symptoms of the disease. The presence of the virus in symptomatic plants was tested and confirmed by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), using a specific PRSV antiserum (Agdia, Elkhart, IN). Total RNA was extracted using RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and tested by reverse transcription-polymerase chain reaction (RT-PCR) with specific PRSV coat protein gene primers: BoCP_Fwd 5?-TCCAAGAATGAAGCTGTGGACGCTGGTT-3? and BoCP_Rev 5?-TYAGTTGCGCATACCCAGGAGAGAGT-3?. The RT-PCR amplicons of the expected size were purified and directly sequenced in both directions at Macrogen Inc. (Seoul, Korea). BLASTn analysis of the sequenced fragments (828 bp) (GenBank accession Nos. KX385113 to KX385116) showed 97 to 98% nucleotide sequence identity with previously reported PRSV-P isolates from Brazil (AF344642, JQ755427, JQ755424). To the best of our knowledge, this is the first report of the occurrence of PRSV in papaya plants in Argentina. Due to the devastating effects of PRSV in papaya (Tripathi et al., 2008) and the recent increase of cultivation area in northern Argentina, it becomes necessary to implement management strategies to control this disease and avoid the introduction of the virus into new production areas.Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Dal Zotto, Angélica. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Portal, Orelvis. Universidad Central ´´marta Abreu´´ de Las Villas; CubaFil: Giolitti, Fabián José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaEdizioni Ets2016-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/180082Cabrera Mederos, Dariel; Dal Zotto, Angélica; Portal, Orelvis; Giolitti, Fabián José; First report of Papaya ringspot virus infecting Carica papaya in Argentina; Edizioni Ets; Journal of Plant Pathology; 98; 3; 7-2016; 687-6871125-4653CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://sipav.org/main/jpp/index.php/jpp/issue/view/149info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:25:42Zoai:ri.conicet.gov.ar:11336/180082instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:25:42.34CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First report of Papaya ringspot virus infecting Carica papaya in Argentina
title First report of Papaya ringspot virus infecting Carica papaya in Argentina
spellingShingle First report of Papaya ringspot virus infecting Carica papaya in Argentina
Cabrera Mederos, Dariel
Papaya ringspot virus
Carica papaya
title_short First report of Papaya ringspot virus infecting Carica papaya in Argentina
title_full First report of Papaya ringspot virus infecting Carica papaya in Argentina
title_fullStr First report of Papaya ringspot virus infecting Carica papaya in Argentina
title_full_unstemmed First report of Papaya ringspot virus infecting Carica papaya in Argentina
title_sort First report of Papaya ringspot virus infecting Carica papaya in Argentina
dc.creator.none.fl_str_mv Cabrera Mederos, Dariel
Dal Zotto, Angélica
Portal, Orelvis
Giolitti, Fabián José
author Cabrera Mederos, Dariel
author_facet Cabrera Mederos, Dariel
Dal Zotto, Angélica
Portal, Orelvis
Giolitti, Fabián José
author_role author
author2 Dal Zotto, Angélica
Portal, Orelvis
Giolitti, Fabián José
author2_role author
author
author
dc.subject.none.fl_str_mv Papaya ringspot virus
Carica papaya
topic Papaya ringspot virus
Carica papaya
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Papaya plants (Carica papaya L.) with severe leaf mosaic and deformation symptoms were observed and collected in the Formosa, Corrientes and Misiones provinces of northern Argentina. These symptoms were similar to those induced by Papaya ringspot virus (PRSV-P) (Tripathi et al., 2008). Electron microscopy observations of leaf dip preparations from symptomatic papaya leaves revealed typical flexuous potyvirus particles of ca. 800×12 nm (Francki et al., 1985). Papaya leaf extracts from affected plants were mechanically inoculated on healthy papaya plants, which showed typical symptoms of the disease. The presence of the virus in symptomatic plants was tested and confirmed by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), using a specific PRSV antiserum (Agdia, Elkhart, IN). Total RNA was extracted using RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and tested by reverse transcription-polymerase chain reaction (RT-PCR) with specific PRSV coat protein gene primers: BoCP_Fwd 5?-TCCAAGAATGAAGCTGTGGACGCTGGTT-3? and BoCP_Rev 5?-TYAGTTGCGCATACCCAGGAGAGAGT-3?. The RT-PCR amplicons of the expected size were purified and directly sequenced in both directions at Macrogen Inc. (Seoul, Korea). BLASTn analysis of the sequenced fragments (828 bp) (GenBank accession Nos. KX385113 to KX385116) showed 97 to 98% nucleotide sequence identity with previously reported PRSV-P isolates from Brazil (AF344642, JQ755427, JQ755424). To the best of our knowledge, this is the first report of the occurrence of PRSV in papaya plants in Argentina. Due to the devastating effects of PRSV in papaya (Tripathi et al., 2008) and the recent increase of cultivation area in northern Argentina, it becomes necessary to implement management strategies to control this disease and avoid the introduction of the virus into new production areas.
Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dal Zotto, Angélica. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Portal, Orelvis. Universidad Central ´´marta Abreu´´ de Las Villas; Cuba
Fil: Giolitti, Fabián José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
description Papaya plants (Carica papaya L.) with severe leaf mosaic and deformation symptoms were observed and collected in the Formosa, Corrientes and Misiones provinces of northern Argentina. These symptoms were similar to those induced by Papaya ringspot virus (PRSV-P) (Tripathi et al., 2008). Electron microscopy observations of leaf dip preparations from symptomatic papaya leaves revealed typical flexuous potyvirus particles of ca. 800×12 nm (Francki et al., 1985). Papaya leaf extracts from affected plants were mechanically inoculated on healthy papaya plants, which showed typical symptoms of the disease. The presence of the virus in symptomatic plants was tested and confirmed by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), using a specific PRSV antiserum (Agdia, Elkhart, IN). Total RNA was extracted using RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and tested by reverse transcription-polymerase chain reaction (RT-PCR) with specific PRSV coat protein gene primers: BoCP_Fwd 5?-TCCAAGAATGAAGCTGTGGACGCTGGTT-3? and BoCP_Rev 5?-TYAGTTGCGCATACCCAGGAGAGAGT-3?. The RT-PCR amplicons of the expected size were purified and directly sequenced in both directions at Macrogen Inc. (Seoul, Korea). BLASTn analysis of the sequenced fragments (828 bp) (GenBank accession Nos. KX385113 to KX385116) showed 97 to 98% nucleotide sequence identity with previously reported PRSV-P isolates from Brazil (AF344642, JQ755427, JQ755424). To the best of our knowledge, this is the first report of the occurrence of PRSV in papaya plants in Argentina. Due to the devastating effects of PRSV in papaya (Tripathi et al., 2008) and the recent increase of cultivation area in northern Argentina, it becomes necessary to implement management strategies to control this disease and avoid the introduction of the virus into new production areas.
publishDate 2016
dc.date.none.fl_str_mv 2016-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/180082
Cabrera Mederos, Dariel; Dal Zotto, Angélica; Portal, Orelvis; Giolitti, Fabián José; First report of Papaya ringspot virus infecting Carica papaya in Argentina; Edizioni Ets; Journal of Plant Pathology; 98; 3; 7-2016; 687-687
1125-4653
CONICET Digital
CONICET
url http://hdl.handle.net/11336/180082
identifier_str_mv Cabrera Mederos, Dariel; Dal Zotto, Angélica; Portal, Orelvis; Giolitti, Fabián José; First report of Papaya ringspot virus infecting Carica papaya in Argentina; Edizioni Ets; Journal of Plant Pathology; 98; 3; 7-2016; 687-687
1125-4653
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://sipav.org/main/jpp/index.php/jpp/issue/view/149
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Edizioni Ets
publisher.none.fl_str_mv Edizioni Ets
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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