Biological and Genomic Characterisation of Curtobacterium flaccumfaciens Infecting Wheat

Autores
Fernandez, Franco Daniel; Martino, Julia Andrea; Pozzi, Elizabeth Alicia; Alberione, Enrique Javier; Tolocka, Patricia; Marquez, Nathalie; Rodriguez, Ana Valeria; Conci, Luis Rogelio; Alemandri, Vanina Maria
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Several phytopathogenic bacteria have been reported worldwide affecting wheat (Triticum aestivum L.), causing significant yield losses. Over the past 5–6 years, symptoms resembling bacterial mosaic, characterised by small yellow lesions with indefinite margins scattered across the leaf, have been observed in wheat plants in one of the main producing areas in Argentina, with an incidence of 10% to 20%. This emerging disease has raised concern among producers and technicians. In this study, a bacterium was isolated from symptomatic seeds, and biochemical, molecular, and genomic techniques were used to identify the causal agent. Pathogenicity tests were conducted on wheat and soybean plants through leaf infiltration, demonstrating that the strain caused disease in wheat but not in soybean. Using a combination of MALDI-TOF MS, the API 20 NE test, 16S rRNA gene, and whole-genome sequencing confirmed the pathogen as Curtobacterium flaccumfaciens. Phylogenomic and genome-to-genome comparisons showed the strain sequenced grouped within the type strain sequence of C. poinsettiae CFB 2403. To our knowledge, this is the first report of Curtobacterium flaccumfaciens as a pathogen on wheat in Argentina. This research contributes significantly to understanding this phytosanitary problem with detailed information on the pathogen, using advanced technology and new reclassifications of pathogenic bacteria, which will allow the development and implementation of effective management strategies in the future.
Instituto de Patología Vegetal
Fil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Fernandez, Franco Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Martino, Julia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Martino, Julia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Pozzi, Elizabeth Alicia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina.
Fil: Alberione, Enrique Javier. Instituto Nacional de Tecnología Agropecuaria (INTA).Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Tolocka, Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Tolocka, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Marquez, Nathalie. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Marquez, Nathalie. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Rodriguez, Ana Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina
Fil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Conci, Luis Rogelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Alemandri, Vanina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Alemandri, Vanina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fuente
Journal of Phytopathology 173 (2): e70049. (First published: 02 March 2025 )
Materia
Cereals
Genomes
Seeds
Wheat
Pathogenic Bacteria
Cereal
Genoma
Semilla
Trigo
Bacteria Patógena
Bacterial Mosaic
Curtobacterium flaccumfaciens
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/21578
Acceder
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oai_identifier_str oai:localhost:20.500.12123/21578
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Biological and Genomic Characterisation of Curtobacterium flaccumfaciens Infecting WheatFernandez, Franco DanielMartino, Julia AndreaPozzi, Elizabeth AliciaAlberione, Enrique JavierTolocka, PatriciaMarquez, NathalieRodriguez, Ana ValeriaConci, Luis RogelioAlemandri, Vanina MariaCerealsGenomesSeedsWheatPathogenic BacteriaCerealGenomaSemillaTrigoBacteria PatógenaBacterial MosaicCurtobacterium flaccumfaciensSeveral phytopathogenic bacteria have been reported worldwide affecting wheat (Triticum aestivum L.), causing significant yield losses. Over the past 5–6 years, symptoms resembling bacterial mosaic, characterised by small yellow lesions with indefinite margins scattered across the leaf, have been observed in wheat plants in one of the main producing areas in Argentina, with an incidence of 10% to 20%. This emerging disease has raised concern among producers and technicians. In this study, a bacterium was isolated from symptomatic seeds, and biochemical, molecular, and genomic techniques were used to identify the causal agent. Pathogenicity tests were conducted on wheat and soybean plants through leaf infiltration, demonstrating that the strain caused disease in wheat but not in soybean. Using a combination of MALDI-TOF MS, the API 20 NE test, 16S rRNA gene, and whole-genome sequencing confirmed the pathogen as Curtobacterium flaccumfaciens. Phylogenomic and genome-to-genome comparisons showed the strain sequenced grouped within the type strain sequence of C. poinsettiae CFB 2403. To our knowledge, this is the first report of Curtobacterium flaccumfaciens as a pathogen on wheat in Argentina. This research contributes significantly to understanding this phytosanitary problem with detailed information on the pathogen, using advanced technology and new reclassifications of pathogenic bacteria, which will allow the development and implementation of effective management strategies in the future.Instituto de Patología VegetalFil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Fernandez, Franco Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Martino, Julia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Martino, Julia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Pozzi, Elizabeth Alicia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina.Fil: Alberione, Enrique Javier. Instituto Nacional de Tecnología Agropecuaria (INTA).Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Tolocka, Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Tolocka, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Marquez, Nathalie. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Marquez, Nathalie. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Rodriguez, Ana Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; ArgentinaFil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Conci, Luis Rogelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Alemandri, Vanina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Alemandri, Vanina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaWiley2025-03-07T09:58:08Z2025-03-07T09:58:08Z2025-02-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21578https://onlinelibrary.wiley.com/doi/10.1111/jph.700490931-17851439-0434 (online)https://doi.org/10.1111/jph.70049Journal of Phytopathology 173 (2): e70049. (First published: 02 March 2025 )reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L03-I084, Estreses bióticos y abióticos en plantas. Estudios fisiológicos y patológicos para el diseño de estrategias de mejoramiento y manejoinfo:eu-repograntAgreement/INTA/2023-PD-L01-I083, Determinación de los mecanismos moleculares que rigen las interacciones entre plantas y patógenos y desarrollo de herramientas para contribuir al control de enfermedadesinfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:32:11Zoai:localhost:20.500.12123/21578instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:32:12.081INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Biological and Genomic Characterisation of Curtobacterium flaccumfaciens Infecting Wheat
title Biological and Genomic Characterisation of Curtobacterium flaccumfaciens Infecting Wheat
spellingShingle Biological and Genomic Characterisation of Curtobacterium flaccumfaciens Infecting Wheat
Fernandez, Franco Daniel
Cereals
Genomes
Seeds
Wheat
Pathogenic Bacteria
Cereal
Genoma
Semilla
Trigo
Bacteria Patógena
Bacterial Mosaic
Curtobacterium flaccumfaciens
title_short Biological and Genomic Characterisation of Curtobacterium flaccumfaciens Infecting Wheat
title_full Biological and Genomic Characterisation of Curtobacterium flaccumfaciens Infecting Wheat
title_fullStr Biological and Genomic Characterisation of Curtobacterium flaccumfaciens Infecting Wheat
title_full_unstemmed Biological and Genomic Characterisation of Curtobacterium flaccumfaciens Infecting Wheat
title_sort Biological and Genomic Characterisation of Curtobacterium flaccumfaciens Infecting Wheat
dc.creator.none.fl_str_mv Fernandez, Franco Daniel
Martino, Julia Andrea
Pozzi, Elizabeth Alicia
Alberione, Enrique Javier
Tolocka, Patricia
Marquez, Nathalie
Rodriguez, Ana Valeria
Conci, Luis Rogelio
Alemandri, Vanina Maria
author Fernandez, Franco Daniel
author_facet Fernandez, Franco Daniel
Martino, Julia Andrea
Pozzi, Elizabeth Alicia
Alberione, Enrique Javier
Tolocka, Patricia
Marquez, Nathalie
Rodriguez, Ana Valeria
Conci, Luis Rogelio
Alemandri, Vanina Maria
author_role author
author2 Martino, Julia Andrea
Pozzi, Elizabeth Alicia
Alberione, Enrique Javier
Tolocka, Patricia
Marquez, Nathalie
Rodriguez, Ana Valeria
Conci, Luis Rogelio
Alemandri, Vanina Maria
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cereals
Genomes
Seeds
Wheat
Pathogenic Bacteria
Cereal
Genoma
Semilla
Trigo
Bacteria Patógena
Bacterial Mosaic
Curtobacterium flaccumfaciens
topic Cereals
Genomes
Seeds
Wheat
Pathogenic Bacteria
Cereal
Genoma
Semilla
Trigo
Bacteria Patógena
Bacterial Mosaic
Curtobacterium flaccumfaciens
dc.description.none.fl_txt_mv Several phytopathogenic bacteria have been reported worldwide affecting wheat (Triticum aestivum L.), causing significant yield losses. Over the past 5–6 years, symptoms resembling bacterial mosaic, characterised by small yellow lesions with indefinite margins scattered across the leaf, have been observed in wheat plants in one of the main producing areas in Argentina, with an incidence of 10% to 20%. This emerging disease has raised concern among producers and technicians. In this study, a bacterium was isolated from symptomatic seeds, and biochemical, molecular, and genomic techniques were used to identify the causal agent. Pathogenicity tests were conducted on wheat and soybean plants through leaf infiltration, demonstrating that the strain caused disease in wheat but not in soybean. Using a combination of MALDI-TOF MS, the API 20 NE test, 16S rRNA gene, and whole-genome sequencing confirmed the pathogen as Curtobacterium flaccumfaciens. Phylogenomic and genome-to-genome comparisons showed the strain sequenced grouped within the type strain sequence of C. poinsettiae CFB 2403. To our knowledge, this is the first report of Curtobacterium flaccumfaciens as a pathogen on wheat in Argentina. This research contributes significantly to understanding this phytosanitary problem with detailed information on the pathogen, using advanced technology and new reclassifications of pathogenic bacteria, which will allow the development and implementation of effective management strategies in the future.
Instituto de Patología Vegetal
Fil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Fernandez, Franco Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Martino, Julia Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Martino, Julia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Pozzi, Elizabeth Alicia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina.
Fil: Alberione, Enrique Javier. Instituto Nacional de Tecnología Agropecuaria (INTA).Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Tolocka, Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Tolocka, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Marquez, Nathalie. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Marquez, Nathalie. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Rodriguez, Ana Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina
Fil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Conci, Luis Rogelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Alemandri, Vanina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Alemandri, Vanina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
description Several phytopathogenic bacteria have been reported worldwide affecting wheat (Triticum aestivum L.), causing significant yield losses. Over the past 5–6 years, symptoms resembling bacterial mosaic, characterised by small yellow lesions with indefinite margins scattered across the leaf, have been observed in wheat plants in one of the main producing areas in Argentina, with an incidence of 10% to 20%. This emerging disease has raised concern among producers and technicians. In this study, a bacterium was isolated from symptomatic seeds, and biochemical, molecular, and genomic techniques were used to identify the causal agent. Pathogenicity tests were conducted on wheat and soybean plants through leaf infiltration, demonstrating that the strain caused disease in wheat but not in soybean. Using a combination of MALDI-TOF MS, the API 20 NE test, 16S rRNA gene, and whole-genome sequencing confirmed the pathogen as Curtobacterium flaccumfaciens. Phylogenomic and genome-to-genome comparisons showed the strain sequenced grouped within the type strain sequence of C. poinsettiae CFB 2403. To our knowledge, this is the first report of Curtobacterium flaccumfaciens as a pathogen on wheat in Argentina. This research contributes significantly to understanding this phytosanitary problem with detailed information on the pathogen, using advanced technology and new reclassifications of pathogenic bacteria, which will allow the development and implementation of effective management strategies in the future.
publishDate 2025
dc.date.none.fl_str_mv 2025-03-07T09:58:08Z
2025-03-07T09:58:08Z
2025-02-20
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/21578
https://onlinelibrary.wiley.com/doi/10.1111/jph.70049
0931-1785
1439-0434 (online)
https://doi.org/10.1111/jph.70049
url http://hdl.handle.net/20.500.12123/21578
https://onlinelibrary.wiley.com/doi/10.1111/jph.70049
https://doi.org/10.1111/jph.70049
identifier_str_mv 0931-1785
1439-0434 (online)
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L03-I084, Estreses bióticos y abióticos en plantas. Estudios fisiológicos y patológicos para el diseño de estrategias de mejoramiento y manejo
info:eu-repograntAgreement/INTA/2023-PD-L01-I083, Determinación de los mecanismos moleculares que rigen las interacciones entre plantas y patógenos y desarrollo de herramientas para contribuir al control de enfermedades
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Journal of Phytopathology 173 (2): e70049. (First published: 02 March 2025 )
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.712165

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