Genomic diversity of phages infecting probiotic strains of Lactobacillus paracasei

Autores
Mercanti, Diego Javier; Rousseau, Geneviève M.; Capra, María Luján; Quiberoni, Andrea del Lujan; Tremblay, Denise M.; Labrie, Simon J.; Moineau, Sylvain
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Strains of the Lactobacillus casei group have been extensively studied because some are used as probiotics in foods. Conversely, their phages have received much less attention. We analyzed the complete genome sequences of five L. paracasei temperate phages: CL1, CL2, iLp84, iLp1308, and iA2. Only phage iA2 could not replicate in an indicator strain. The genome lengths ranged from 34,155 bp (iA2) to 39,474 bp (CL1). Phages iA2 and iLp1308 (34,176 bp) possess the smallest genomes reported, thus far, for phages of the L. casei group. The GC contents of the five phage genomes ranged from 44.8 to 45.6%. As observed with many other phages, their genomes were organized as follows: Genes coding for DNA packaging, morphogenesis, lysis, lysogeny, and replication. Phages CL1, CL2, and iLp1308 are highly related to each other. Phage iLp84 was also related to these three phages, but the similarities were limited to gene products involved in DNA packaging and structural proteins. Genomic fragments of phages CL1, CL2, iLp1308, and iLp84 were found in several genomes of L. casei strains. Prophage iA2 is unrelated to these four phages, but almost all of its genome was found in at least four L. casei strains. Overall, these phages are distinct from previously characterized Lactobacillus phages. Our results highlight the diversity of L. casei phages and indicate frequent DNA exchanges between phages and their hosts.
Fil: Mercanti, Diego Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Rousseau, Geneviève M.. Faculté des Sciences et de Génie; Canadá. Universite Laval; Francia
Fil: Capra, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Tremblay, Denise M.. Faculté des Sciences et de Génie; Canadá. Universite Laval; Francia
Fil: Labrie, Simon J.. Faculté des Sciences et de Génie; Canadá. Universite Laval; Francia
Fil: Moineau, Sylvain. Faculté des Sciences et de Génie; Canadá. Universite Laval; Francia
Materia
BACTERIOPHAGES
LACTOBACILLUS
GENOME ANALYSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/50576
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/50576
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genomic diversity of phages infecting probiotic strains of Lactobacillus paracaseiMercanti, Diego JavierRousseau, Geneviève M.Capra, María LujánQuiberoni, Andrea del LujanTremblay, Denise M.Labrie, Simon J.Moineau, SylvainBACTERIOPHAGESLACTOBACILLUSGENOME ANALYSIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Strains of the Lactobacillus casei group have been extensively studied because some are used as probiotics in foods. Conversely, their phages have received much less attention. We analyzed the complete genome sequences of five L. paracasei temperate phages: CL1, CL2, iLp84, iLp1308, and iA2. Only phage iA2 could not replicate in an indicator strain. The genome lengths ranged from 34,155 bp (iA2) to 39,474 bp (CL1). Phages iA2 and iLp1308 (34,176 bp) possess the smallest genomes reported, thus far, for phages of the L. casei group. The GC contents of the five phage genomes ranged from 44.8 to 45.6%. As observed with many other phages, their genomes were organized as follows: Genes coding for DNA packaging, morphogenesis, lysis, lysogeny, and replication. Phages CL1, CL2, and iLp1308 are highly related to each other. Phage iLp84 was also related to these three phages, but the similarities were limited to gene products involved in DNA packaging and structural proteins. Genomic fragments of phages CL1, CL2, iLp1308, and iLp84 were found in several genomes of L. casei strains. Prophage iA2 is unrelated to these four phages, but almost all of its genome was found in at least four L. casei strains. Overall, these phages are distinct from previously characterized Lactobacillus phages. Our results highlight the diversity of L. casei phages and indicate frequent DNA exchanges between phages and their hosts.Fil: Mercanti, Diego Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Rousseau, Geneviève M.. Faculté des Sciences et de Génie; Canadá. Universite Laval; FranciaFil: Capra, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Tremblay, Denise M.. Faculté des Sciences et de Génie; Canadá. Universite Laval; FranciaFil: Labrie, Simon J.. Faculté des Sciences et de Génie; Canadá. Universite Laval; FranciaFil: Moineau, Sylvain. Faculté des Sciences et de Génie; Canadá. Universite Laval; FranciaAmerican Society for Microbiology2015-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/50576Mercanti, Diego Javier; Rousseau, Geneviève M.; Capra, María Luján; Quiberoni, Andrea del Lujan; Tremblay, Denise M.; et al.; Genomic diversity of phages infecting probiotic strains of Lactobacillus paracasei; American Society for Microbiology; Applied And Environmental Microbiology; 82; 1; 10-2015; 95-1050099-2240CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.02723-15info:eu-repo/semantics/altIdentifier/url/http://aem.asm.org/content/82/1/95info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:33:47Zoai:ri.conicet.gov.ar:11336/50576instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:33:47.36CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genomic diversity of phages infecting probiotic strains of Lactobacillus paracasei
title Genomic diversity of phages infecting probiotic strains of Lactobacillus paracasei
spellingShingle Genomic diversity of phages infecting probiotic strains of Lactobacillus paracasei
Mercanti, Diego Javier
BACTERIOPHAGES
LACTOBACILLUS
GENOME ANALYSIS
title_short Genomic diversity of phages infecting probiotic strains of Lactobacillus paracasei
title_full Genomic diversity of phages infecting probiotic strains of Lactobacillus paracasei
title_fullStr Genomic diversity of phages infecting probiotic strains of Lactobacillus paracasei
title_full_unstemmed Genomic diversity of phages infecting probiotic strains of Lactobacillus paracasei
title_sort Genomic diversity of phages infecting probiotic strains of Lactobacillus paracasei
dc.creator.none.fl_str_mv Mercanti, Diego Javier
Rousseau, Geneviève M.
Capra, María Luján
Quiberoni, Andrea del Lujan
Tremblay, Denise M.
Labrie, Simon J.
Moineau, Sylvain
author Mercanti, Diego Javier
author_facet Mercanti, Diego Javier
Rousseau, Geneviève M.
Capra, María Luján
Quiberoni, Andrea del Lujan
Tremblay, Denise M.
Labrie, Simon J.
Moineau, Sylvain
author_role author
author2 Rousseau, Geneviève M.
Capra, María Luján
Quiberoni, Andrea del Lujan
Tremblay, Denise M.
Labrie, Simon J.
Moineau, Sylvain
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BACTERIOPHAGES
LACTOBACILLUS
GENOME ANALYSIS
topic BACTERIOPHAGES
LACTOBACILLUS
GENOME ANALYSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Strains of the Lactobacillus casei group have been extensively studied because some are used as probiotics in foods. Conversely, their phages have received much less attention. We analyzed the complete genome sequences of five L. paracasei temperate phages: CL1, CL2, iLp84, iLp1308, and iA2. Only phage iA2 could not replicate in an indicator strain. The genome lengths ranged from 34,155 bp (iA2) to 39,474 bp (CL1). Phages iA2 and iLp1308 (34,176 bp) possess the smallest genomes reported, thus far, for phages of the L. casei group. The GC contents of the five phage genomes ranged from 44.8 to 45.6%. As observed with many other phages, their genomes were organized as follows: Genes coding for DNA packaging, morphogenesis, lysis, lysogeny, and replication. Phages CL1, CL2, and iLp1308 are highly related to each other. Phage iLp84 was also related to these three phages, but the similarities were limited to gene products involved in DNA packaging and structural proteins. Genomic fragments of phages CL1, CL2, iLp1308, and iLp84 were found in several genomes of L. casei strains. Prophage iA2 is unrelated to these four phages, but almost all of its genome was found in at least four L. casei strains. Overall, these phages are distinct from previously characterized Lactobacillus phages. Our results highlight the diversity of L. casei phages and indicate frequent DNA exchanges between phages and their hosts.
Fil: Mercanti, Diego Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Rousseau, Geneviève M.. Faculté des Sciences et de Génie; Canadá. Universite Laval; Francia
Fil: Capra, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Tremblay, Denise M.. Faculté des Sciences et de Génie; Canadá. Universite Laval; Francia
Fil: Labrie, Simon J.. Faculté des Sciences et de Génie; Canadá. Universite Laval; Francia
Fil: Moineau, Sylvain. Faculté des Sciences et de Génie; Canadá. Universite Laval; Francia
description Strains of the Lactobacillus casei group have been extensively studied because some are used as probiotics in foods. Conversely, their phages have received much less attention. We analyzed the complete genome sequences of five L. paracasei temperate phages: CL1, CL2, iLp84, iLp1308, and iA2. Only phage iA2 could not replicate in an indicator strain. The genome lengths ranged from 34,155 bp (iA2) to 39,474 bp (CL1). Phages iA2 and iLp1308 (34,176 bp) possess the smallest genomes reported, thus far, for phages of the L. casei group. The GC contents of the five phage genomes ranged from 44.8 to 45.6%. As observed with many other phages, their genomes were organized as follows: Genes coding for DNA packaging, morphogenesis, lysis, lysogeny, and replication. Phages CL1, CL2, and iLp1308 are highly related to each other. Phage iLp84 was also related to these three phages, but the similarities were limited to gene products involved in DNA packaging and structural proteins. Genomic fragments of phages CL1, CL2, iLp1308, and iLp84 were found in several genomes of L. casei strains. Prophage iA2 is unrelated to these four phages, but almost all of its genome was found in at least four L. casei strains. Overall, these phages are distinct from previously characterized Lactobacillus phages. Our results highlight the diversity of L. casei phages and indicate frequent DNA exchanges between phages and their hosts.
publishDate 2015
dc.date.none.fl_str_mv 2015-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/50576
Mercanti, Diego Javier; Rousseau, Geneviève M.; Capra, María Luján; Quiberoni, Andrea del Lujan; Tremblay, Denise M.; et al.; Genomic diversity of phages infecting probiotic strains of Lactobacillus paracasei; American Society for Microbiology; Applied And Environmental Microbiology; 82; 1; 10-2015; 95-105
0099-2240
CONICET Digital
CONICET
url http://hdl.handle.net/11336/50576
identifier_str_mv Mercanti, Diego Javier; Rousseau, Geneviève M.; Capra, María Luján; Quiberoni, Andrea del Lujan; Tremblay, Denise M.; et al.; Genomic diversity of phages infecting probiotic strains of Lactobacillus paracasei; American Society for Microbiology; Applied And Environmental Microbiology; 82; 1; 10-2015; 95-105
0099-2240
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.02723-15
info:eu-repo/semantics/altIdentifier/url/http://aem.asm.org/content/82/1/95
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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