Characterization of sensitivity of grove and packing house isolates of Penicillium digitatum to pyrimethanil

Autores
Vazquez, Daniel Eduardo; Panozzo, Marina Graciela; Almirón, Nanci Jacqueline; Bello, Fernando; Burdyn, Lourdes; Garran, Sergio Mario
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In most northeast Argentinean citrus packing houses, postharvest fungicide treatments are based onthe use of thiabendazole and imazalil. However, these fungicides have been used in a manner highlyconducive to the selection and proliferation of resistant biotypes of Penicillium digitatum, the main fruitdecay fungus in the area. Recently, a new fungicide, pyrimethanil (PYR), was introduced to control molds.Aims of this study were to determine the baseline sensitivities for PYR against isolates of P. digitatumconsidering its use in the region is not yet widespread and to evaluate the control of the fungus in vivo.One hundred and nine (109) P. digitatum isolates were collected from diseased fruit within citrus groves(43 isolates) and packing houses (66 isolates). EC50was determined for each isolate by measuring colonydiameters on different agar dilutions of the fungicide. The mean EC50value of the green mold isolatescollected from the groves was 0.14 ± 0.03 mg L−1while the mean EC50of those collected from packinghouses was 0.13 ± 0.05 mg L−1. No resistant isolates were found in the field where the fungicide is notused, while one isolate originated from a packing house showed an EC50of 3.40 mg L−1, 26-fold higherthan the mean level. This isolate was collected from lemons stored in cool rooms of a packing house wherePYR had not been used. Fruit decay by sensitive isolates was reduced approximately 80% by PYR appliedat 500–600 mg L−1by immersion for 60 s at room temperature to inoculated oranges and mandarins. Incontrast, the resistant isolate was not controlled by PYR applied at 1000 mg L−1. Thus, the introduction ofPYR applied into packing houses should be done carefully and control strategies should be implementedin order to minimize the development of resistant isolates.
EEA Concordia
Fil: Vazquez, Daniel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Panozzo, Marina Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Almiron, Nanci Jacqueline. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Burdyn, Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Garran, Sergio Mario. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fuente
Postharvest biology and technology 98 : 1–6. (2014)
Materia
Resistencia a los Fungicidas
Penicillium Digitatum
Citrus
Contaminación Biológica
Tecnología Postcosecha
Postharvest Technology
Packaging
Resistance to Fungicides
Empaquetado
Pyrimethanil
Baseline Sensitivity
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2832
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2832
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network_name_str INTA Digital (INTA)
spelling Characterization of sensitivity of grove and packing house isolates of Penicillium digitatum to pyrimethanilVazquez, Daniel EduardoPanozzo, Marina GracielaAlmirón, Nanci JacquelineBello, FernandoBurdyn, LourdesGarran, Sergio MarioResistencia a los FungicidasPenicillium DigitatumCitrusContaminación BiológicaTecnología PostcosechaPostharvest TechnologyPackagingResistance to FungicidesEmpaquetadoPyrimethanilBaseline SensitivityIn most northeast Argentinean citrus packing houses, postharvest fungicide treatments are based onthe use of thiabendazole and imazalil. However, these fungicides have been used in a manner highlyconducive to the selection and proliferation of resistant biotypes of Penicillium digitatum, the main fruitdecay fungus in the area. Recently, a new fungicide, pyrimethanil (PYR), was introduced to control molds.Aims of this study were to determine the baseline sensitivities for PYR against isolates of P. digitatumconsidering its use in the region is not yet widespread and to evaluate the control of the fungus in vivo.One hundred and nine (109) P. digitatum isolates were collected from diseased fruit within citrus groves(43 isolates) and packing houses (66 isolates). EC50was determined for each isolate by measuring colonydiameters on different agar dilutions of the fungicide. The mean EC50value of the green mold isolatescollected from the groves was 0.14 ± 0.03 mg L−1while the mean EC50of those collected from packinghouses was 0.13 ± 0.05 mg L−1. No resistant isolates were found in the field where the fungicide is notused, while one isolate originated from a packing house showed an EC50of 3.40 mg L−1, 26-fold higherthan the mean level. This isolate was collected from lemons stored in cool rooms of a packing house wherePYR had not been used. Fruit decay by sensitive isolates was reduced approximately 80% by PYR appliedat 500–600 mg L−1by immersion for 60 s at room temperature to inoculated oranges and mandarins. Incontrast, the resistant isolate was not controlled by PYR applied at 1000 mg L−1. Thus, the introduction ofPYR applied into packing houses should be done carefully and control strategies should be implementedin order to minimize the development of resistant isolates.EEA ConcordiaFil: Vazquez, Daniel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Panozzo, Marina Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Almiron, Nanci Jacqueline. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Burdyn, Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Garran, Sergio Mario. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina2018-07-19T18:10:14Z2018-07-19T18:10:14Z2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/28320925-5214https://doi.org/10.1016/j.postharvbio.2014.06.012Postharvest biology and technology 98 : 1–6. (2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-12-18T09:01:00Zoai:localhost:20.500.12123/2832instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-12-18 09:01:00.915INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Characterization of sensitivity of grove and packing house isolates of Penicillium digitatum to pyrimethanil
title Characterization of sensitivity of grove and packing house isolates of Penicillium digitatum to pyrimethanil
spellingShingle Characterization of sensitivity of grove and packing house isolates of Penicillium digitatum to pyrimethanil
Vazquez, Daniel Eduardo
Resistencia a los Fungicidas
Penicillium Digitatum
Citrus
Contaminación Biológica
Tecnología Postcosecha
Postharvest Technology
Packaging
Resistance to Fungicides
Empaquetado
Pyrimethanil
Baseline Sensitivity
title_short Characterization of sensitivity of grove and packing house isolates of Penicillium digitatum to pyrimethanil
title_full Characterization of sensitivity of grove and packing house isolates of Penicillium digitatum to pyrimethanil
title_fullStr Characterization of sensitivity of grove and packing house isolates of Penicillium digitatum to pyrimethanil
title_full_unstemmed Characterization of sensitivity of grove and packing house isolates of Penicillium digitatum to pyrimethanil
title_sort Characterization of sensitivity of grove and packing house isolates of Penicillium digitatum to pyrimethanil
dc.creator.none.fl_str_mv Vazquez, Daniel Eduardo
Panozzo, Marina Graciela
Almirón, Nanci Jacqueline
Bello, Fernando
Burdyn, Lourdes
Garran, Sergio Mario
author Vazquez, Daniel Eduardo
author_facet Vazquez, Daniel Eduardo
Panozzo, Marina Graciela
Almirón, Nanci Jacqueline
Bello, Fernando
Burdyn, Lourdes
Garran, Sergio Mario
author_role author
author2 Panozzo, Marina Graciela
Almirón, Nanci Jacqueline
Bello, Fernando
Burdyn, Lourdes
Garran, Sergio Mario
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Resistencia a los Fungicidas
Penicillium Digitatum
Citrus
Contaminación Biológica
Tecnología Postcosecha
Postharvest Technology
Packaging
Resistance to Fungicides
Empaquetado
Pyrimethanil
Baseline Sensitivity
topic Resistencia a los Fungicidas
Penicillium Digitatum
Citrus
Contaminación Biológica
Tecnología Postcosecha
Postharvest Technology
Packaging
Resistance to Fungicides
Empaquetado
Pyrimethanil
Baseline Sensitivity
dc.description.none.fl_txt_mv In most northeast Argentinean citrus packing houses, postharvest fungicide treatments are based onthe use of thiabendazole and imazalil. However, these fungicides have been used in a manner highlyconducive to the selection and proliferation of resistant biotypes of Penicillium digitatum, the main fruitdecay fungus in the area. Recently, a new fungicide, pyrimethanil (PYR), was introduced to control molds.Aims of this study were to determine the baseline sensitivities for PYR against isolates of P. digitatumconsidering its use in the region is not yet widespread and to evaluate the control of the fungus in vivo.One hundred and nine (109) P. digitatum isolates were collected from diseased fruit within citrus groves(43 isolates) and packing houses (66 isolates). EC50was determined for each isolate by measuring colonydiameters on different agar dilutions of the fungicide. The mean EC50value of the green mold isolatescollected from the groves was 0.14 ± 0.03 mg L−1while the mean EC50of those collected from packinghouses was 0.13 ± 0.05 mg L−1. No resistant isolates were found in the field where the fungicide is notused, while one isolate originated from a packing house showed an EC50of 3.40 mg L−1, 26-fold higherthan the mean level. This isolate was collected from lemons stored in cool rooms of a packing house wherePYR had not been used. Fruit decay by sensitive isolates was reduced approximately 80% by PYR appliedat 500–600 mg L−1by immersion for 60 s at room temperature to inoculated oranges and mandarins. Incontrast, the resistant isolate was not controlled by PYR applied at 1000 mg L−1. Thus, the introduction ofPYR applied into packing houses should be done carefully and control strategies should be implementedin order to minimize the development of resistant isolates.
EEA Concordia
Fil: Vazquez, Daniel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Panozzo, Marina Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Almiron, Nanci Jacqueline. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Burdyn, Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Garran, Sergio Mario. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
description In most northeast Argentinean citrus packing houses, postharvest fungicide treatments are based onthe use of thiabendazole and imazalil. However, these fungicides have been used in a manner highlyconducive to the selection and proliferation of resistant biotypes of Penicillium digitatum, the main fruitdecay fungus in the area. Recently, a new fungicide, pyrimethanil (PYR), was introduced to control molds.Aims of this study were to determine the baseline sensitivities for PYR against isolates of P. digitatumconsidering its use in the region is not yet widespread and to evaluate the control of the fungus in vivo.One hundred and nine (109) P. digitatum isolates were collected from diseased fruit within citrus groves(43 isolates) and packing houses (66 isolates). EC50was determined for each isolate by measuring colonydiameters on different agar dilutions of the fungicide. The mean EC50value of the green mold isolatescollected from the groves was 0.14 ± 0.03 mg L−1while the mean EC50of those collected from packinghouses was 0.13 ± 0.05 mg L−1. No resistant isolates were found in the field where the fungicide is notused, while one isolate originated from a packing house showed an EC50of 3.40 mg L−1, 26-fold higherthan the mean level. This isolate was collected from lemons stored in cool rooms of a packing house wherePYR had not been used. Fruit decay by sensitive isolates was reduced approximately 80% by PYR appliedat 500–600 mg L−1by immersion for 60 s at room temperature to inoculated oranges and mandarins. Incontrast, the resistant isolate was not controlled by PYR applied at 1000 mg L−1. Thus, the introduction ofPYR applied into packing houses should be done carefully and control strategies should be implementedin order to minimize the development of resistant isolates.
publishDate 2014
dc.date.none.fl_str_mv 2014
2018-07-19T18:10:14Z
2018-07-19T18:10:14Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/2832
0925-5214
https://doi.org/10.1016/j.postharvbio.2014.06.012
url http://hdl.handle.net/20.500.12123/2832
https://doi.org/10.1016/j.postharvbio.2014.06.012
identifier_str_mv 0925-5214
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Postharvest biology and technology 98 : 1–6. (2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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