Screening methods for isolation of biocontrol epiphytic yeasts against penicillium digitatum in lemons

Autores
Pereyra, Martina María; Diaz, Mariana Andrea; Soliz Santander, Fabricio Fabián; Poehlein, Anja; Meinhardt, Friedhelm; Daniel, Rolf; Dib, Julian Rafael
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Worldwide, the green rot caused by Penicillium digitatum is one of the most aggressive postharvest diseases of lemons. Searching for sustainable alternatives to chemical fungicides, epi-phytic yeasts as potential biocontrol agents were isolated from citrus fruits using a tailor-made selective medium. For disclosing their antagonistic potential against P. digitatum, obtained isolates were subjected to direct screening methods, both in vitro and in vivo. In the course of the primary in vitro screening that comprised dual culture assays, 43 yeast strains displaying antagonistic activities against the pathogen were selected. Subsequently, such strains were subjected to an in vivo screening that consisted of a microscale test, allowing the selection of six yeast strains for further analysis. In the final screening using macroscale in vivo tests, three strains (AcL2, AgL21, and AgL2) displaying the highest efficiencies to control P. digitatum were identified. The protection efficiencies in lemons were 80 (AcL2), 76.7 (AgL21), and 75% (AgL2). Based on sequence analysis of the PCR amplified D1/D2 domains of the 26S rRNA genes, they were identified as representatives of the species Clavispora lusitaniae. Interestingly, the strains exhibited a broad action spectrum among citrus fruits as they were also able to combat the green mold disease in grapefruit and two orange varieties. The direct screening methods applied in this study favored the recovery of effi-cient candidates for application as biological control agents to combat fungal infestations of citrus fruits.
Fil: Pereyra, Martina María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Diaz, Mariana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Soliz Santander, Fabricio Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Poehlein, Anja. Universität Göttingen; Alemania
Fil: Meinhardt, Friedhelm. Westfälische Wilhelms Universität; Alemania
Fil: Daniel, Rolf. Universität Göttingen; Alemania
Fil: Dib, Julian Rafael. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Materia
BIOLOGICAL CONTROL
CITRUS FRUITS
EPIPHYTIC YEASTS
PENICILLIUM DIGITATUM
SCREENING METHODS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/133779
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/133779
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Screening methods for isolation of biocontrol epiphytic yeasts against penicillium digitatum in lemonsPereyra, Martina MaríaDiaz, Mariana AndreaSoliz Santander, Fabricio FabiánPoehlein, AnjaMeinhardt, FriedhelmDaniel, RolfDib, Julian RafaelBIOLOGICAL CONTROLCITRUS FRUITSEPIPHYTIC YEASTSPENICILLIUM DIGITATUMSCREENING METHODShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Worldwide, the green rot caused by Penicillium digitatum is one of the most aggressive postharvest diseases of lemons. Searching for sustainable alternatives to chemical fungicides, epi-phytic yeasts as potential biocontrol agents were isolated from citrus fruits using a tailor-made selective medium. For disclosing their antagonistic potential against P. digitatum, obtained isolates were subjected to direct screening methods, both in vitro and in vivo. In the course of the primary in vitro screening that comprised dual culture assays, 43 yeast strains displaying antagonistic activities against the pathogen were selected. Subsequently, such strains were subjected to an in vivo screening that consisted of a microscale test, allowing the selection of six yeast strains for further analysis. In the final screening using macroscale in vivo tests, three strains (AcL2, AgL21, and AgL2) displaying the highest efficiencies to control P. digitatum were identified. The protection efficiencies in lemons were 80 (AcL2), 76.7 (AgL21), and 75% (AgL2). Based on sequence analysis of the PCR amplified D1/D2 domains of the 26S rRNA genes, they were identified as representatives of the species Clavispora lusitaniae. Interestingly, the strains exhibited a broad action spectrum among citrus fruits as they were also able to combat the green mold disease in grapefruit and two orange varieties. The direct screening methods applied in this study favored the recovery of effi-cient candidates for application as biological control agents to combat fungal infestations of citrus fruits.Fil: Pereyra, Martina María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Diaz, Mariana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Soliz Santander, Fabricio Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Poehlein, Anja. Universität Göttingen; AlemaniaFil: Meinhardt, Friedhelm. Westfälische Wilhelms Universität; AlemaniaFil: Daniel, Rolf. Universität Göttingen; AlemaniaFil: Dib, Julian Rafael. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaMDPI AG2021-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/133779Pereyra, Martina María; Diaz, Mariana Andrea; Soliz Santander, Fabricio Fabián; Poehlein, Anja; Meinhardt, Friedhelm; et al.; Screening methods for isolation of biocontrol epiphytic yeasts against penicillium digitatum in lemons; MDPI AG; Journal of Fungi; 7; 3; 3-2021; 1-132309-608XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2309-608X/7/3/166info:eu-repo/semantics/altIdentifier/doi/10.3390/jof7030166info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:14:14Zoai:ri.conicet.gov.ar:11336/133779instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:14:14.397CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Screening methods for isolation of biocontrol epiphytic yeasts against penicillium digitatum in lemons
title Screening methods for isolation of biocontrol epiphytic yeasts against penicillium digitatum in lemons
spellingShingle Screening methods for isolation of biocontrol epiphytic yeasts against penicillium digitatum in lemons
Pereyra, Martina María
BIOLOGICAL CONTROL
CITRUS FRUITS
EPIPHYTIC YEASTS
PENICILLIUM DIGITATUM
SCREENING METHODS
title_short Screening methods for isolation of biocontrol epiphytic yeasts against penicillium digitatum in lemons
title_full Screening methods for isolation of biocontrol epiphytic yeasts against penicillium digitatum in lemons
title_fullStr Screening methods for isolation of biocontrol epiphytic yeasts against penicillium digitatum in lemons
title_full_unstemmed Screening methods for isolation of biocontrol epiphytic yeasts against penicillium digitatum in lemons
title_sort Screening methods for isolation of biocontrol epiphytic yeasts against penicillium digitatum in lemons
dc.creator.none.fl_str_mv Pereyra, Martina María
Diaz, Mariana Andrea
Soliz Santander, Fabricio Fabián
Poehlein, Anja
Meinhardt, Friedhelm
Daniel, Rolf
Dib, Julian Rafael
author Pereyra, Martina María
author_facet Pereyra, Martina María
Diaz, Mariana Andrea
Soliz Santander, Fabricio Fabián
Poehlein, Anja
Meinhardt, Friedhelm
Daniel, Rolf
Dib, Julian Rafael
author_role author
author2 Diaz, Mariana Andrea
Soliz Santander, Fabricio Fabián
Poehlein, Anja
Meinhardt, Friedhelm
Daniel, Rolf
Dib, Julian Rafael
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOLOGICAL CONTROL
CITRUS FRUITS
EPIPHYTIC YEASTS
PENICILLIUM DIGITATUM
SCREENING METHODS
topic BIOLOGICAL CONTROL
CITRUS FRUITS
EPIPHYTIC YEASTS
PENICILLIUM DIGITATUM
SCREENING METHODS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Worldwide, the green rot caused by Penicillium digitatum is one of the most aggressive postharvest diseases of lemons. Searching for sustainable alternatives to chemical fungicides, epi-phytic yeasts as potential biocontrol agents were isolated from citrus fruits using a tailor-made selective medium. For disclosing their antagonistic potential against P. digitatum, obtained isolates were subjected to direct screening methods, both in vitro and in vivo. In the course of the primary in vitro screening that comprised dual culture assays, 43 yeast strains displaying antagonistic activities against the pathogen were selected. Subsequently, such strains were subjected to an in vivo screening that consisted of a microscale test, allowing the selection of six yeast strains for further analysis. In the final screening using macroscale in vivo tests, three strains (AcL2, AgL21, and AgL2) displaying the highest efficiencies to control P. digitatum were identified. The protection efficiencies in lemons were 80 (AcL2), 76.7 (AgL21), and 75% (AgL2). Based on sequence analysis of the PCR amplified D1/D2 domains of the 26S rRNA genes, they were identified as representatives of the species Clavispora lusitaniae. Interestingly, the strains exhibited a broad action spectrum among citrus fruits as they were also able to combat the green mold disease in grapefruit and two orange varieties. The direct screening methods applied in this study favored the recovery of effi-cient candidates for application as biological control agents to combat fungal infestations of citrus fruits.
Fil: Pereyra, Martina María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Diaz, Mariana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Soliz Santander, Fabricio Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Poehlein, Anja. Universität Göttingen; Alemania
Fil: Meinhardt, Friedhelm. Westfälische Wilhelms Universität; Alemania
Fil: Daniel, Rolf. Universität Göttingen; Alemania
Fil: Dib, Julian Rafael. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
description Worldwide, the green rot caused by Penicillium digitatum is one of the most aggressive postharvest diseases of lemons. Searching for sustainable alternatives to chemical fungicides, epi-phytic yeasts as potential biocontrol agents were isolated from citrus fruits using a tailor-made selective medium. For disclosing their antagonistic potential against P. digitatum, obtained isolates were subjected to direct screening methods, both in vitro and in vivo. In the course of the primary in vitro screening that comprised dual culture assays, 43 yeast strains displaying antagonistic activities against the pathogen were selected. Subsequently, such strains were subjected to an in vivo screening that consisted of a microscale test, allowing the selection of six yeast strains for further analysis. In the final screening using macroscale in vivo tests, three strains (AcL2, AgL21, and AgL2) displaying the highest efficiencies to control P. digitatum were identified. The protection efficiencies in lemons were 80 (AcL2), 76.7 (AgL21), and 75% (AgL2). Based on sequence analysis of the PCR amplified D1/D2 domains of the 26S rRNA genes, they were identified as representatives of the species Clavispora lusitaniae. Interestingly, the strains exhibited a broad action spectrum among citrus fruits as they were also able to combat the green mold disease in grapefruit and two orange varieties. The direct screening methods applied in this study favored the recovery of effi-cient candidates for application as biological control agents to combat fungal infestations of citrus fruits.
publishDate 2021
dc.date.none.fl_str_mv 2021-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/133779
Pereyra, Martina María; Diaz, Mariana Andrea; Soliz Santander, Fabricio Fabián; Poehlein, Anja; Meinhardt, Friedhelm; et al.; Screening methods for isolation of biocontrol epiphytic yeasts against penicillium digitatum in lemons; MDPI AG; Journal of Fungi; 7; 3; 3-2021; 1-13
2309-608X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/133779
identifier_str_mv Pereyra, Martina María; Diaz, Mariana Andrea; Soliz Santander, Fabricio Fabián; Poehlein, Anja; Meinhardt, Friedhelm; et al.; Screening methods for isolation of biocontrol epiphytic yeasts against penicillium digitatum in lemons; MDPI AG; Journal of Fungi; 7; 3; 3-2021; 1-13
2309-608X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2309-608X/7/3/166
info:eu-repo/semantics/altIdentifier/doi/10.3390/jof7030166
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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