In vivo and in vitro antagonism of Streptomyces sp. RO3 against Penicillium digitatum and Geotrichum candidum

Autores
Maldonado, Maria Cristina; Orosco, Carlos Exequiel Francisco; Gordillo, Maria; Navarro, Antonio Roberto
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Streptomyces strains may control postharvest lemon fruit diseases caused by Penicillium digitatum and Geotrichum candidum. Four Streptomyces strains (RO3, MC3, Alem and CE1) were tested and RO3 was the strain with the highest antagonistic activity. Streptomyces RO3 showed a maximum biomass value (2.15 g/L) after 72 h incubation in yeast extract, malt extract, glucose (YMG) medium; in starch casein medium the highest value was 0.477 g/L at 120 h incubation. The metabolites produced in YMG medium showed the maximum inhibition against G. candidum and P. digitatum (19.64 and 62.12% respectively). The inhibitory activity of metabolites decreased after autoclaving. RO3 metabolites have molecular mass higher than 2000 and they have fungicidal action mode against P. digitatum. In vivo assays, RO3 metabolites with wax, totally controlled lemon diseases (mainly sour rot and green mold). This is the first study on Streptomyces to control postharvest lemon diseases.
Fil: Maldonado, Maria Cristina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Orosco, Carlos Exequiel Francisco. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Gordillo, Maria. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Navarro, Antonio Roberto. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Materia
Streptomyces
Geotrichum candidum
metabolites, lemon diseases
Penicillium digitatum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/77469
Acceder
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network_name_str CONICET Digital (CONICET)
spelling In vivo and in vitro antagonism of Streptomyces sp. RO3 against Penicillium digitatum and Geotrichum candidumMaldonado, Maria CristinaOrosco, Carlos Exequiel FranciscoGordillo, MariaNavarro, Antonio RobertoStreptomycesGeotrichum candidummetabolites, lemon diseasesPenicillium digitatumhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Streptomyces strains may control postharvest lemon fruit diseases caused by Penicillium digitatum and Geotrichum candidum. Four Streptomyces strains (RO3, MC3, Alem and CE1) were tested and RO3 was the strain with the highest antagonistic activity. Streptomyces RO3 showed a maximum biomass value (2.15 g/L) after 72 h incubation in yeast extract, malt extract, glucose (YMG) medium; in starch casein medium the highest value was 0.477 g/L at 120 h incubation. The metabolites produced in YMG medium showed the maximum inhibition against G. candidum and P. digitatum (19.64 and 62.12% respectively). The inhibitory activity of metabolites decreased after autoclaving. RO3 metabolites have molecular mass higher than 2000 and they have fungicidal action mode against P. digitatum. In vivo assays, RO3 metabolites with wax, totally controlled lemon diseases (mainly sour rot and green mold). This is the first study on Streptomyces to control postharvest lemon diseases.Fil: Maldonado, Maria Cristina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Orosco, Carlos Exequiel Francisco. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Gordillo, Maria. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Navarro, Antonio Roberto. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; ArgentinaAcademic Journals2010-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/77469Maldonado, Maria Cristina; Orosco, Carlos Exequiel Francisco; Gordillo, Maria; Navarro, Antonio Roberto; In vivo and in vitro antagonism of Streptomyces sp. RO3 against Penicillium digitatum and Geotrichum candidum; Academic Journals; African Journal Of Microbiology Research; 4; 22; 11-2010; 2451-24561996-0808CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.5897/AJMRinfo:eu-repo/semantics/altIdentifier/url/https://academicjournals.org/journal/AJMR/article-abstract/BFE312E15694info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:07:16Zoai:ri.conicet.gov.ar:11336/77469instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:07:16.468CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vivo and in vitro antagonism of Streptomyces sp. RO3 against Penicillium digitatum and Geotrichum candidum
title In vivo and in vitro antagonism of Streptomyces sp. RO3 against Penicillium digitatum and Geotrichum candidum
spellingShingle In vivo and in vitro antagonism of Streptomyces sp. RO3 against Penicillium digitatum and Geotrichum candidum
Maldonado, Maria Cristina
Streptomyces
Geotrichum candidum
metabolites, lemon diseases
Penicillium digitatum
title_short In vivo and in vitro antagonism of Streptomyces sp. RO3 against Penicillium digitatum and Geotrichum candidum
title_full In vivo and in vitro antagonism of Streptomyces sp. RO3 against Penicillium digitatum and Geotrichum candidum
title_fullStr In vivo and in vitro antagonism of Streptomyces sp. RO3 against Penicillium digitatum and Geotrichum candidum
title_full_unstemmed In vivo and in vitro antagonism of Streptomyces sp. RO3 against Penicillium digitatum and Geotrichum candidum
title_sort In vivo and in vitro antagonism of Streptomyces sp. RO3 against Penicillium digitatum and Geotrichum candidum
dc.creator.none.fl_str_mv Maldonado, Maria Cristina
Orosco, Carlos Exequiel Francisco
Gordillo, Maria
Navarro, Antonio Roberto
author Maldonado, Maria Cristina
author_facet Maldonado, Maria Cristina
Orosco, Carlos Exequiel Francisco
Gordillo, Maria
Navarro, Antonio Roberto
author_role author
author2 Orosco, Carlos Exequiel Francisco
Gordillo, Maria
Navarro, Antonio Roberto
author2_role author
author
author
dc.subject.none.fl_str_mv Streptomyces
Geotrichum candidum
metabolites, lemon diseases
Penicillium digitatum
topic Streptomyces
Geotrichum candidum
metabolites, lemon diseases
Penicillium digitatum
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Streptomyces strains may control postharvest lemon fruit diseases caused by Penicillium digitatum and Geotrichum candidum. Four Streptomyces strains (RO3, MC3, Alem and CE1) were tested and RO3 was the strain with the highest antagonistic activity. Streptomyces RO3 showed a maximum biomass value (2.15 g/L) after 72 h incubation in yeast extract, malt extract, glucose (YMG) medium; in starch casein medium the highest value was 0.477 g/L at 120 h incubation. The metabolites produced in YMG medium showed the maximum inhibition against G. candidum and P. digitatum (19.64 and 62.12% respectively). The inhibitory activity of metabolites decreased after autoclaving. RO3 metabolites have molecular mass higher than 2000 and they have fungicidal action mode against P. digitatum. In vivo assays, RO3 metabolites with wax, totally controlled lemon diseases (mainly sour rot and green mold). This is the first study on Streptomyces to control postharvest lemon diseases.
Fil: Maldonado, Maria Cristina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Orosco, Carlos Exequiel Francisco. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Gordillo, Maria. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Navarro, Antonio Roberto. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
description Streptomyces strains may control postharvest lemon fruit diseases caused by Penicillium digitatum and Geotrichum candidum. Four Streptomyces strains (RO3, MC3, Alem and CE1) were tested and RO3 was the strain with the highest antagonistic activity. Streptomyces RO3 showed a maximum biomass value (2.15 g/L) after 72 h incubation in yeast extract, malt extract, glucose (YMG) medium; in starch casein medium the highest value was 0.477 g/L at 120 h incubation. The metabolites produced in YMG medium showed the maximum inhibition against G. candidum and P. digitatum (19.64 and 62.12% respectively). The inhibitory activity of metabolites decreased after autoclaving. RO3 metabolites have molecular mass higher than 2000 and they have fungicidal action mode against P. digitatum. In vivo assays, RO3 metabolites with wax, totally controlled lemon diseases (mainly sour rot and green mold). This is the first study on Streptomyces to control postharvest lemon diseases.
publishDate 2010
dc.date.none.fl_str_mv 2010-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/77469
Maldonado, Maria Cristina; Orosco, Carlos Exequiel Francisco; Gordillo, Maria; Navarro, Antonio Roberto; In vivo and in vitro antagonism of Streptomyces sp. RO3 against Penicillium digitatum and Geotrichum candidum; Academic Journals; African Journal Of Microbiology Research; 4; 22; 11-2010; 2451-2456
1996-0808
CONICET Digital
CONICET
url http://hdl.handle.net/11336/77469
identifier_str_mv Maldonado, Maria Cristina; Orosco, Carlos Exequiel Francisco; Gordillo, Maria; Navarro, Antonio Roberto; In vivo and in vitro antagonism of Streptomyces sp. RO3 against Penicillium digitatum and Geotrichum candidum; Academic Journals; African Journal Of Microbiology Research; 4; 22; 11-2010; 2451-2456
1996-0808
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.5897/AJMR
info:eu-repo/semantics/altIdentifier/url/https://academicjournals.org/journal/AJMR/article-abstract/BFE312E15694
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Journals
publisher.none.fl_str_mv Academic Journals
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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