Detection of ovine antibody to Brucella ovis by Indirect Enzyme Immunoassay

Autores
Nielsen, Klaus; Smith, P.; Yu, W. L.; Rojas, X.; Perez, B.; Conde, Sandra Beatriz; Samartino, Luis Ernesto; Robles, Carlos Alejandro
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Because some batch-to-batch variation in the preparation of rough lipopolysaccharide (RLPS) from Brucella ovis has been experienced, several protocols were tested to establish the most reliable method for detection of antibody in indirect enzyme immunoassay. An early version of the assay gave a performance index (PI ¼ sum of optimum percent sensitivity and percent specificity, determined by receiver operator characteristic analysis) of 198.6. This assay used RLPS from B. ovis as the antigen and a monoclonal antibody specific for bovine IgG1 heavy chain-enzyme conjugate for detection. This was not repeatable using other batches of antigen. Newer versions of the assay generally had decreased sensitivity values, giving PIs of 193. Use of a recombinant protein A/G-enzyme conjugate did not improve the PI (PI ¼ 190), giving reduced specificity and higher sensitivity. The final version used B. abortus RB51 RLPS as the antigen and protein A/G-enzyme conjugate for detection, giving a PI of 197. Because of the batch uniformity of the B. abortus RB51 RLPS and the versatility of the protein A/G-enzyme conjugate, the latter version appears to be the most useful for diagnostic serology.
Estación Experimental Agropecuaria Bariloche
Fil: Nielsen, Klaus. Canadian Food Inspection Agency. Ottawa Laboratories; Canadá
Fil: Smith, P. Canadian Food Inspection Agency. Ottawa Laboratories; Canadá
Fil: Yu, W. L. Canadian Food Inspection Agency. Ottawa Laboratories; Canadá
Fil: Rojas, X. Universidad Austral de Chile. Instituto de Microbiología; Chile
Fil: Perez, B. Servicio Agrícola y Ganadero; Chile
Fil: Conde, Sandra Beatriz. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Agencia de Extensión Rural Lobos; Argentina
Fil: Samartino, Luis Ernesto. Instituto Nacional de Tecnologia Agropecuaria (INTA); Argentina
Fil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Sanidad Animal; Argentina
Fuente
Journal of Immunoassay and Immunochemistry 28 : 243-250 (2007)
Materia
Anticuerpos
Brucelosis
Brucella
Brucella Ovis
Técnicas Inmunológicas
Antibodies
Brucellosis
Immunological Techniques
Inmunoensayo
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/12414
Acceder
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oai_identifier_str oai:localhost:20.500.12123/12414
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Detection of ovine antibody to Brucella ovis by Indirect Enzyme ImmunoassayNielsen, KlausSmith, P.Yu, W. L.Rojas, X.Perez, B.Conde, Sandra BeatrizSamartino, Luis ErnestoRobles, Carlos AlejandroAnticuerposBrucelosisBrucellaBrucella OvisTécnicas InmunológicasAntibodiesBrucellosisImmunological TechniquesInmunoensayoBecause some batch-to-batch variation in the preparation of rough lipopolysaccharide (RLPS) from Brucella ovis has been experienced, several protocols were tested to establish the most reliable method for detection of antibody in indirect enzyme immunoassay. An early version of the assay gave a performance index (PI ¼ sum of optimum percent sensitivity and percent specificity, determined by receiver operator characteristic analysis) of 198.6. This assay used RLPS from B. ovis as the antigen and a monoclonal antibody specific for bovine IgG1 heavy chain-enzyme conjugate for detection. This was not repeatable using other batches of antigen. Newer versions of the assay generally had decreased sensitivity values, giving PIs of 193. Use of a recombinant protein A/G-enzyme conjugate did not improve the PI (PI ¼ 190), giving reduced specificity and higher sensitivity. The final version used B. abortus RB51 RLPS as the antigen and protein A/G-enzyme conjugate for detection, giving a PI of 197. Because of the batch uniformity of the B. abortus RB51 RLPS and the versatility of the protein A/G-enzyme conjugate, the latter version appears to be the most useful for diagnostic serology.Estación Experimental Agropecuaria BarilocheFil: Nielsen, Klaus. Canadian Food Inspection Agency. Ottawa Laboratories; CanadáFil: Smith, P. Canadian Food Inspection Agency. Ottawa Laboratories; CanadáFil: Yu, W. L. Canadian Food Inspection Agency. Ottawa Laboratories; CanadáFil: Rojas, X. Universidad Austral de Chile. Instituto de Microbiología; ChileFil: Perez, B. Servicio Agrícola y Ganadero; ChileFil: Conde, Sandra Beatriz. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Agencia de Extensión Rural Lobos; ArgentinaFil: Samartino, Luis Ernesto. Instituto Nacional de Tecnologia Agropecuaria (INTA); ArgentinaFil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Sanidad Animal; ArgentinaTaylor & Francis Group2022-07-27T10:56:32Z2022-07-27T10:56:32Z2007info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/12414https://www.tandfonline.com/doi/abs/10.1080/153218107014547891532-18191532-4230https://doi.org/10.1080/15321810701454789Journal of Immunoassay and Immunochemistry 28 : 243-250 (2007)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:38Zoai:localhost:20.500.12123/12414instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:38.652INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Detection of ovine antibody to Brucella ovis by Indirect Enzyme Immunoassay
title Detection of ovine antibody to Brucella ovis by Indirect Enzyme Immunoassay
spellingShingle Detection of ovine antibody to Brucella ovis by Indirect Enzyme Immunoassay
Nielsen, Klaus
Anticuerpos
Brucelosis
Brucella
Brucella Ovis
Técnicas Inmunológicas
Antibodies
Brucellosis
Immunological Techniques
Inmunoensayo
title_short Detection of ovine antibody to Brucella ovis by Indirect Enzyme Immunoassay
title_full Detection of ovine antibody to Brucella ovis by Indirect Enzyme Immunoassay
title_fullStr Detection of ovine antibody to Brucella ovis by Indirect Enzyme Immunoassay
title_full_unstemmed Detection of ovine antibody to Brucella ovis by Indirect Enzyme Immunoassay
title_sort Detection of ovine antibody to Brucella ovis by Indirect Enzyme Immunoassay
dc.creator.none.fl_str_mv Nielsen, Klaus
Smith, P.
Yu, W. L.
Rojas, X.
Perez, B.
Conde, Sandra Beatriz
Samartino, Luis Ernesto
Robles, Carlos Alejandro
author Nielsen, Klaus
author_facet Nielsen, Klaus
Smith, P.
Yu, W. L.
Rojas, X.
Perez, B.
Conde, Sandra Beatriz
Samartino, Luis Ernesto
Robles, Carlos Alejandro
author_role author
author2 Smith, P.
Yu, W. L.
Rojas, X.
Perez, B.
Conde, Sandra Beatriz
Samartino, Luis Ernesto
Robles, Carlos Alejandro
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Anticuerpos
Brucelosis
Brucella
Brucella Ovis
Técnicas Inmunológicas
Antibodies
Brucellosis
Immunological Techniques
Inmunoensayo
topic Anticuerpos
Brucelosis
Brucella
Brucella Ovis
Técnicas Inmunológicas
Antibodies
Brucellosis
Immunological Techniques
Inmunoensayo
dc.description.none.fl_txt_mv Because some batch-to-batch variation in the preparation of rough lipopolysaccharide (RLPS) from Brucella ovis has been experienced, several protocols were tested to establish the most reliable method for detection of antibody in indirect enzyme immunoassay. An early version of the assay gave a performance index (PI ¼ sum of optimum percent sensitivity and percent specificity, determined by receiver operator characteristic analysis) of 198.6. This assay used RLPS from B. ovis as the antigen and a monoclonal antibody specific for bovine IgG1 heavy chain-enzyme conjugate for detection. This was not repeatable using other batches of antigen. Newer versions of the assay generally had decreased sensitivity values, giving PIs of 193. Use of a recombinant protein A/G-enzyme conjugate did not improve the PI (PI ¼ 190), giving reduced specificity and higher sensitivity. The final version used B. abortus RB51 RLPS as the antigen and protein A/G-enzyme conjugate for detection, giving a PI of 197. Because of the batch uniformity of the B. abortus RB51 RLPS and the versatility of the protein A/G-enzyme conjugate, the latter version appears to be the most useful for diagnostic serology.
Estación Experimental Agropecuaria Bariloche
Fil: Nielsen, Klaus. Canadian Food Inspection Agency. Ottawa Laboratories; Canadá
Fil: Smith, P. Canadian Food Inspection Agency. Ottawa Laboratories; Canadá
Fil: Yu, W. L. Canadian Food Inspection Agency. Ottawa Laboratories; Canadá
Fil: Rojas, X. Universidad Austral de Chile. Instituto de Microbiología; Chile
Fil: Perez, B. Servicio Agrícola y Ganadero; Chile
Fil: Conde, Sandra Beatriz. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Agencia de Extensión Rural Lobos; Argentina
Fil: Samartino, Luis Ernesto. Instituto Nacional de Tecnologia Agropecuaria (INTA); Argentina
Fil: Robles, Carlos Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Sanidad Animal; Argentina
description Because some batch-to-batch variation in the preparation of rough lipopolysaccharide (RLPS) from Brucella ovis has been experienced, several protocols were tested to establish the most reliable method for detection of antibody in indirect enzyme immunoassay. An early version of the assay gave a performance index (PI ¼ sum of optimum percent sensitivity and percent specificity, determined by receiver operator characteristic analysis) of 198.6. This assay used RLPS from B. ovis as the antigen and a monoclonal antibody specific for bovine IgG1 heavy chain-enzyme conjugate for detection. This was not repeatable using other batches of antigen. Newer versions of the assay generally had decreased sensitivity values, giving PIs of 193. Use of a recombinant protein A/G-enzyme conjugate did not improve the PI (PI ¼ 190), giving reduced specificity and higher sensitivity. The final version used B. abortus RB51 RLPS as the antigen and protein A/G-enzyme conjugate for detection, giving a PI of 197. Because of the batch uniformity of the B. abortus RB51 RLPS and the versatility of the protein A/G-enzyme conjugate, the latter version appears to be the most useful for diagnostic serology.
publishDate 2007
dc.date.none.fl_str_mv 2007
2022-07-27T10:56:32Z
2022-07-27T10:56:32Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/12414
https://www.tandfonline.com/doi/abs/10.1080/15321810701454789
1532-1819
1532-4230
https://doi.org/10.1080/15321810701454789
url http://hdl.handle.net/20.500.12123/12414
https://www.tandfonline.com/doi/abs/10.1080/15321810701454789
https://doi.org/10.1080/15321810701454789
identifier_str_mv 1532-1819
1532-4230
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis Group
publisher.none.fl_str_mv Taylor & Francis Group
dc.source.none.fl_str_mv Journal of Immunoassay and Immunochemistry 28 : 243-250 (2007)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1844619167289835520
score 12.891075

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