Use of the c-terminal coding region of Ligb for the molecular diagnosis (Ligbct PCR) of bovine leptospirosis in serum
- Autores
- Saraullo, Vanina Rosa; Hamer, Micaela; Esteban, Micaela; Sanchez, Maria Cristina; Brihuega, Bibiana Felicitas; Martinez, Mara Leila
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Poster
Argentina is an endemic country with epidemic outbreaks of leptospirosis. In lives tock, reproductive failures are knowing. A recent study considers that Leptospira is the second most common abortigenic pathogen and is responsible for more than US$ 7 million of losses in the cattle and dairy industry, respectively (1). In this context, it is essential to have sensitive and specific tools in order to effectively control the disease the spread. Molecular tools are widely used in different biological samples In this work, LigBct PCR which amplifies a 756 bp region of the C-terminal end of the conserved LigB protein (2) (ligbct) was used to detect leptospiral DNA in bovine serum. We used 134 bovine sera from the Leptospirosis Laboratory of INTA Castelar (OMSA Reference Laboratory) which arrived with a suspected diagnosis of Leptospirosis. All sera were classified by MAT (Microagglutination Test) as positive (n: 89) or non-de tectable ND (n: 45) with a cut-off point equal to 1/200. LigBct PCR was performed according to the protocol standardized by Saraullo et al (3). LigBct PCR was able to detect leptospiral DNA in 78.65% of positive sera, and in 42.22% of sera classified as ND by MAT. In the face of positive titers by MAT, remanent leptospiral DNA in the bloodstream can still be detected by LigBct PCR. However, leptospiral DNA was also detected in 42.22% of samples where antibodies were ND by MAT. High MAT titers infer infection as opposed to lower titers or ND, often without visible symptomato logy. Animals may not present symptoms compatible with leptospirosis, have low or undetectable titers with TMA, but be carriers and disseminate the bacteria through urine infecting the environment, other animals, and humans; therefore, the present technique could be serve to detect carrier animals. It is considered that LigBct PCR could be a complementary tool to detect leptospiral DNA in serum. The development of new diagnostic methods is of utmost importance in order to carry out an ade quate diagnosis of leptospirosis in Argentina and thus contribute to the reduction of the great economic losses associated with this important zoonotic disease.
Instituto de Patobiología
Fil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Esteban, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Esteban, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sanchez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Sanchez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brihuega, Bibiana Felicitas. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias. Instituto de Investigación en Veterinaria; Argentina
Fil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina
Fil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martinez, Mara Leila. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias. Instituto de Investigación en Veterinaria; Argentina - Fuente
- The 13th Conference of the International Leptospirosis Society and The 4th Meeting of the European Leptospirosis and other Roden-Born Haemorrhagic fevers Society, Brussels, 2-4 September 2024
- Materia
-
Laboratory Diagnosis
Leptospirosis
Cattle
Immunoenzyme Techniques
Diagnóstico de Laboratorio
Ganado Bovino
Técnicas Inmunoenzimáticas
PCR
Argentina - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
.jpg)
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/20485
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Use of the c-terminal coding region of Ligb for the molecular diagnosis (Ligbct PCR) of bovine leptospirosis in serumSaraullo, Vanina RosaHamer, MicaelaEsteban, MicaelaSanchez, Maria CristinaBrihuega, Bibiana FelicitasMartinez, Mara LeilaLaboratory DiagnosisLeptospirosisCattleImmunoenzyme TechniquesDiagnóstico de LaboratorioGanado BovinoTécnicas InmunoenzimáticasPCRArgentinaPosterArgentina is an endemic country with epidemic outbreaks of leptospirosis. In lives tock, reproductive failures are knowing. A recent study considers that Leptospira is the second most common abortigenic pathogen and is responsible for more than US$ 7 million of losses in the cattle and dairy industry, respectively (1). In this context, it is essential to have sensitive and specific tools in order to effectively control the disease the spread. Molecular tools are widely used in different biological samples In this work, LigBct PCR which amplifies a 756 bp region of the C-terminal end of the conserved LigB protein (2) (ligbct) was used to detect leptospiral DNA in bovine serum. We used 134 bovine sera from the Leptospirosis Laboratory of INTA Castelar (OMSA Reference Laboratory) which arrived with a suspected diagnosis of Leptospirosis. All sera were classified by MAT (Microagglutination Test) as positive (n: 89) or non-de tectable ND (n: 45) with a cut-off point equal to 1/200. LigBct PCR was performed according to the protocol standardized by Saraullo et al (3). LigBct PCR was able to detect leptospiral DNA in 78.65% of positive sera, and in 42.22% of sera classified as ND by MAT. In the face of positive titers by MAT, remanent leptospiral DNA in the bloodstream can still be detected by LigBct PCR. However, leptospiral DNA was also detected in 42.22% of samples where antibodies were ND by MAT. High MAT titers infer infection as opposed to lower titers or ND, often without visible symptomato logy. Animals may not present symptoms compatible with leptospirosis, have low or undetectable titers with TMA, but be carriers and disseminate the bacteria through urine infecting the environment, other animals, and humans; therefore, the present technique could be serve to detect carrier animals. It is considered that LigBct PCR could be a complementary tool to detect leptospiral DNA in serum. The development of new diagnostic methods is of utmost importance in order to carry out an ade quate diagnosis of leptospirosis in Argentina and thus contribute to the reduction of the great economic losses associated with this important zoonotic disease.Instituto de PatobiologíaFil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; ArgentinaFil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; ArgentinaFil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Esteban, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; ArgentinaFil: Esteban, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sanchez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; ArgentinaFil: Sanchez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; ArgentinaFil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Brihuega, Bibiana Felicitas. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias. Instituto de Investigación en Veterinaria; ArgentinaFil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; ArgentinaFil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Martinez, Mara Leila. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias. Instituto de Investigación en Veterinaria; ArgentinaInternational Leptospirosis Society2024-12-05T11:08:51Z2024-12-05T11:08:51Z2024-09info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfhttp://hdl.handle.net/20.500.12123/20485The 13th Conference of the International Leptospirosis Society and The 4th Meeting of the European Leptospirosis and other Roden-Born Haemorrhagic fevers Society, Brussels, 2-4 September 2024reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:50:47Zoai:localhost:20.500.12123/20485instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:50:48.199INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
| dc.title.none.fl_str_mv |
Use of the c-terminal coding region of Ligb for the molecular diagnosis (Ligbct PCR) of bovine leptospirosis in serum |
| title |
Use of the c-terminal coding region of Ligb for the molecular diagnosis (Ligbct PCR) of bovine leptospirosis in serum |
| spellingShingle |
Use of the c-terminal coding region of Ligb for the molecular diagnosis (Ligbct PCR) of bovine leptospirosis in serum Saraullo, Vanina Rosa Laboratory Diagnosis Leptospirosis Cattle Immunoenzyme Techniques Diagnóstico de Laboratorio Ganado Bovino Técnicas Inmunoenzimáticas PCR Argentina |
| title_short |
Use of the c-terminal coding region of Ligb for the molecular diagnosis (Ligbct PCR) of bovine leptospirosis in serum |
| title_full |
Use of the c-terminal coding region of Ligb for the molecular diagnosis (Ligbct PCR) of bovine leptospirosis in serum |
| title_fullStr |
Use of the c-terminal coding region of Ligb for the molecular diagnosis (Ligbct PCR) of bovine leptospirosis in serum |
| title_full_unstemmed |
Use of the c-terminal coding region of Ligb for the molecular diagnosis (Ligbct PCR) of bovine leptospirosis in serum |
| title_sort |
Use of the c-terminal coding region of Ligb for the molecular diagnosis (Ligbct PCR) of bovine leptospirosis in serum |
| dc.creator.none.fl_str_mv |
Saraullo, Vanina Rosa Hamer, Micaela Esteban, Micaela Sanchez, Maria Cristina Brihuega, Bibiana Felicitas Martinez, Mara Leila |
| author |
Saraullo, Vanina Rosa |
| author_facet |
Saraullo, Vanina Rosa Hamer, Micaela Esteban, Micaela Sanchez, Maria Cristina Brihuega, Bibiana Felicitas Martinez, Mara Leila |
| author_role |
author |
| author2 |
Hamer, Micaela Esteban, Micaela Sanchez, Maria Cristina Brihuega, Bibiana Felicitas Martinez, Mara Leila |
| author2_role |
author author author author author |
| dc.subject.none.fl_str_mv |
Laboratory Diagnosis Leptospirosis Cattle Immunoenzyme Techniques Diagnóstico de Laboratorio Ganado Bovino Técnicas Inmunoenzimáticas PCR Argentina |
| topic |
Laboratory Diagnosis Leptospirosis Cattle Immunoenzyme Techniques Diagnóstico de Laboratorio Ganado Bovino Técnicas Inmunoenzimáticas PCR Argentina |
| dc.description.none.fl_txt_mv |
Poster Argentina is an endemic country with epidemic outbreaks of leptospirosis. In lives tock, reproductive failures are knowing. A recent study considers that Leptospira is the second most common abortigenic pathogen and is responsible for more than US$ 7 million of losses in the cattle and dairy industry, respectively (1). In this context, it is essential to have sensitive and specific tools in order to effectively control the disease the spread. Molecular tools are widely used in different biological samples In this work, LigBct PCR which amplifies a 756 bp region of the C-terminal end of the conserved LigB protein (2) (ligbct) was used to detect leptospiral DNA in bovine serum. We used 134 bovine sera from the Leptospirosis Laboratory of INTA Castelar (OMSA Reference Laboratory) which arrived with a suspected diagnosis of Leptospirosis. All sera were classified by MAT (Microagglutination Test) as positive (n: 89) or non-de tectable ND (n: 45) with a cut-off point equal to 1/200. LigBct PCR was performed according to the protocol standardized by Saraullo et al (3). LigBct PCR was able to detect leptospiral DNA in 78.65% of positive sera, and in 42.22% of sera classified as ND by MAT. In the face of positive titers by MAT, remanent leptospiral DNA in the bloodstream can still be detected by LigBct PCR. However, leptospiral DNA was also detected in 42.22% of samples where antibodies were ND by MAT. High MAT titers infer infection as opposed to lower titers or ND, often without visible symptomato logy. Animals may not present symptoms compatible with leptospirosis, have low or undetectable titers with TMA, but be carriers and disseminate the bacteria through urine infecting the environment, other animals, and humans; therefore, the present technique could be serve to detect carrier animals. It is considered that LigBct PCR could be a complementary tool to detect leptospiral DNA in serum. The development of new diagnostic methods is of utmost importance in order to carry out an ade quate diagnosis of leptospirosis in Argentina and thus contribute to the reduction of the great economic losses associated with this important zoonotic disease. Instituto de Patobiología Fil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Esteban, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Esteban, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sanchez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Sanchez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Brihuega, Bibiana Felicitas. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias. Instituto de Investigación en Veterinaria; Argentina Fil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria. Laboratorio de Leptospirosis; Argentina Fil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Martinez, Mara Leila. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias. Instituto de Investigación en Veterinaria; Argentina |
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Poster |
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2024 |
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eng |
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International Leptospirosis Society |
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The 13th Conference of the International Leptospirosis Society and The 4th Meeting of the European Leptospirosis and other Roden-Born Haemorrhagic fevers Society, Brussels, 2-4 September 2024 reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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