New enzyme-linked immunoassay for the detection of specific antibodies against multiple Leptospira serogroups in bovine sera

Autores
Martinez, Mara Leila; Rodriguez, Marcelo A.; Irazu, Lucía E.; Romero, Graciela Noemi; Saraullo, Vanina Rosa; Watanabe, Olivia; Hamer, Micaela; Grune Loffler, Sylvia; Samartino, Luis Ernesto; Brihuega, Bibiana Felicitas
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Leptospirosis is a zoonotic disease with worldwide endemicity in Argentina, it is a significant public health problem in low-income populations. Bovine leptospirosis is a serious economic problem for cattle production, causing abortions, reduced milk yield, mortality in calves and decreased daily weight gain. We developed an enzyme-linked immunosorbent assay (ELISA) with sonicated Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni M 20. We evaluated its performance for the detection of specific antibodies against multiple Leptospira serogroups in bovine. The microscopic agglutination test (MAT) was used as the gold standard. The performance of this ELISA was evaluated with a panel of sera (118 MAT confirmed positive and 97 MAT negative). The overall sensitivity was close to 85.6 % and the specificity was 83.5 %, according to the MAT reference method. Analytical specificity of the IgG-ELISA was evaluated using 50 bovine serum samples from animals showing serum antibodies against other pathogens that cause abortion in bovine, such as Brucella sp., Neospora sp. and Bovine Viral Diarrhea (BVD), and no cross-reaction was observed. This IgG-ELISA can be an alternative to the MAT for diagnosis of leptospiral infection in bovine.
Instituto de Patobiología
Fil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Rodriguez, Marcelo A. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos Malbrán”. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Irazu, Lucía E. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos Malbrán”. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Romero, Graciela Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Watanabe, Olivia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Grune Loffler, Sylvia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Samartino, Luis Ernesto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romero, Graciela Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Watanabe, Olivia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Grune Loffler, Sylvia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Samartino, Luis Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Comparative immunology, microbiology and infectious ddiseases 75 : 101609. (Abril 2021)
Materia
ELISA
Bovinae
Leptospira
Leptospirosis
Diagnóstico
Aborto
Enzimas
Técnicas Inmunológicas
Anticuerpos
Diagnosis
Abortion
Enzymes
Immunological Techniques
Antibodies
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/8652
Acceder
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oai_identifier_str oai:localhost:20.500.12123/8652
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling New enzyme-linked immunoassay for the detection of specific antibodies against multiple Leptospira serogroups in bovine seraMartinez, Mara LeilaRodriguez, Marcelo A.Irazu, Lucía E.Romero, Graciela NoemiSaraullo, Vanina RosaWatanabe, OliviaHamer, MicaelaGrune Loffler, SylviaSamartino, Luis ErnestoBrihuega, Bibiana FelicitasELISABovinaeLeptospiraLeptospirosisDiagnósticoAbortoEnzimasTécnicas InmunológicasAnticuerposDiagnosisAbortionEnzymesImmunological TechniquesAntibodiesLeptospirosis is a zoonotic disease with worldwide endemicity in Argentina, it is a significant public health problem in low-income populations. Bovine leptospirosis is a serious economic problem for cattle production, causing abortions, reduced milk yield, mortality in calves and decreased daily weight gain. We developed an enzyme-linked immunosorbent assay (ELISA) with sonicated Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni M 20. We evaluated its performance for the detection of specific antibodies against multiple Leptospira serogroups in bovine. The microscopic agglutination test (MAT) was used as the gold standard. The performance of this ELISA was evaluated with a panel of sera (118 MAT confirmed positive and 97 MAT negative). The overall sensitivity was close to 85.6 % and the specificity was 83.5 %, according to the MAT reference method. Analytical specificity of the IgG-ELISA was evaluated using 50 bovine serum samples from animals showing serum antibodies against other pathogens that cause abortion in bovine, such as Brucella sp., Neospora sp. and Bovine Viral Diarrhea (BVD), and no cross-reaction was observed. This IgG-ELISA can be an alternative to the MAT for diagnosis of leptospiral infection in bovine.Instituto de PatobiologíaFil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.Fil: Rodriguez, Marcelo A. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos Malbrán”. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Irazu, Lucía E. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos Malbrán”. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Romero, Graciela Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.Fil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.Fil: Watanabe, Olivia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.Fil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.Fil: Grune Loffler, Sylvia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.Fil: Samartino, Luis Ernesto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Romero, Graciela Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Watanabe, Olivia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Grune Loffler, Sylvia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Samartino, Luis Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2021-01-26T15:01:16Z2021-01-26T15:01:16Z2021-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8652https://www.sciencedirect.com/science/article/abs/pii/S01479571210000111878-1667https://doi.org/10.1016/j.cimid.2021.101609Comparative immunology, microbiology and infectious ddiseases 75 : 101609. (Abril 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:59Zoai:localhost:20.500.12123/8652instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:30:00.199INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv New enzyme-linked immunoassay for the detection of specific antibodies against multiple Leptospira serogroups in bovine sera
title New enzyme-linked immunoassay for the detection of specific antibodies against multiple Leptospira serogroups in bovine sera
spellingShingle New enzyme-linked immunoassay for the detection of specific antibodies against multiple Leptospira serogroups in bovine sera
Martinez, Mara Leila
ELISA
Bovinae
Leptospira
Leptospirosis
Diagnóstico
Aborto
Enzimas
Técnicas Inmunológicas
Anticuerpos
Diagnosis
Abortion
Enzymes
Immunological Techniques
Antibodies
title_short New enzyme-linked immunoassay for the detection of specific antibodies against multiple Leptospira serogroups in bovine sera
title_full New enzyme-linked immunoassay for the detection of specific antibodies against multiple Leptospira serogroups in bovine sera
title_fullStr New enzyme-linked immunoassay for the detection of specific antibodies against multiple Leptospira serogroups in bovine sera
title_full_unstemmed New enzyme-linked immunoassay for the detection of specific antibodies against multiple Leptospira serogroups in bovine sera
title_sort New enzyme-linked immunoassay for the detection of specific antibodies against multiple Leptospira serogroups in bovine sera
dc.creator.none.fl_str_mv Martinez, Mara Leila
Rodriguez, Marcelo A.
Irazu, Lucía E.
Romero, Graciela Noemi
Saraullo, Vanina Rosa
Watanabe, Olivia
Hamer, Micaela
Grune Loffler, Sylvia
Samartino, Luis Ernesto
Brihuega, Bibiana Felicitas
author Martinez, Mara Leila
author_facet Martinez, Mara Leila
Rodriguez, Marcelo A.
Irazu, Lucía E.
Romero, Graciela Noemi
Saraullo, Vanina Rosa
Watanabe, Olivia
Hamer, Micaela
Grune Loffler, Sylvia
Samartino, Luis Ernesto
Brihuega, Bibiana Felicitas
author_role author
author2 Rodriguez, Marcelo A.
Irazu, Lucía E.
Romero, Graciela Noemi
Saraullo, Vanina Rosa
Watanabe, Olivia
Hamer, Micaela
Grune Loffler, Sylvia
Samartino, Luis Ernesto
Brihuega, Bibiana Felicitas
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ELISA
Bovinae
Leptospira
Leptospirosis
Diagnóstico
Aborto
Enzimas
Técnicas Inmunológicas
Anticuerpos
Diagnosis
Abortion
Enzymes
Immunological Techniques
Antibodies
topic ELISA
Bovinae
Leptospira
Leptospirosis
Diagnóstico
Aborto
Enzimas
Técnicas Inmunológicas
Anticuerpos
Diagnosis
Abortion
Enzymes
Immunological Techniques
Antibodies
dc.description.none.fl_txt_mv Leptospirosis is a zoonotic disease with worldwide endemicity in Argentina, it is a significant public health problem in low-income populations. Bovine leptospirosis is a serious economic problem for cattle production, causing abortions, reduced milk yield, mortality in calves and decreased daily weight gain. We developed an enzyme-linked immunosorbent assay (ELISA) with sonicated Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni M 20. We evaluated its performance for the detection of specific antibodies against multiple Leptospira serogroups in bovine. The microscopic agglutination test (MAT) was used as the gold standard. The performance of this ELISA was evaluated with a panel of sera (118 MAT confirmed positive and 97 MAT negative). The overall sensitivity was close to 85.6 % and the specificity was 83.5 %, according to the MAT reference method. Analytical specificity of the IgG-ELISA was evaluated using 50 bovine serum samples from animals showing serum antibodies against other pathogens that cause abortion in bovine, such as Brucella sp., Neospora sp. and Bovine Viral Diarrhea (BVD), and no cross-reaction was observed. This IgG-ELISA can be an alternative to the MAT for diagnosis of leptospiral infection in bovine.
Instituto de Patobiología
Fil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Rodriguez, Marcelo A. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos Malbrán”. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Irazu, Lucía E. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos Malbrán”. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Romero, Graciela Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Watanabe, Olivia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Grune Loffler, Sylvia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Samartino, Luis Ernesto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina.
Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romero, Graciela Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Watanabe, Olivia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Grune Loffler, Sylvia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Samartino, Luis Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Leptospirosis is a zoonotic disease with worldwide endemicity in Argentina, it is a significant public health problem in low-income populations. Bovine leptospirosis is a serious economic problem for cattle production, causing abortions, reduced milk yield, mortality in calves and decreased daily weight gain. We developed an enzyme-linked immunosorbent assay (ELISA) with sonicated Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni M 20. We evaluated its performance for the detection of specific antibodies against multiple Leptospira serogroups in bovine. The microscopic agglutination test (MAT) was used as the gold standard. The performance of this ELISA was evaluated with a panel of sera (118 MAT confirmed positive and 97 MAT negative). The overall sensitivity was close to 85.6 % and the specificity was 83.5 %, according to the MAT reference method. Analytical specificity of the IgG-ELISA was evaluated using 50 bovine serum samples from animals showing serum antibodies against other pathogens that cause abortion in bovine, such as Brucella sp., Neospora sp. and Bovine Viral Diarrhea (BVD), and no cross-reaction was observed. This IgG-ELISA can be an alternative to the MAT for diagnosis of leptospiral infection in bovine.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-26T15:01:16Z
2021-01-26T15:01:16Z
2021-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/8652
https://www.sciencedirect.com/science/article/abs/pii/S0147957121000011
1878-1667
https://doi.org/10.1016/j.cimid.2021.101609
url http://hdl.handle.net/20.500.12123/8652
https://www.sciencedirect.com/science/article/abs/pii/S0147957121000011
https://doi.org/10.1016/j.cimid.2021.101609
identifier_str_mv 1878-1667
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Comparative immunology, microbiology and infectious ddiseases 75 : 101609. (Abril 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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