Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with abovine rotavirus VP6 experimental vaccine

Autores
Gonzalez, Diego; Rimondi, Agustina; Perez Aguirreburialde, Maria Sol; Mozgovoj, Marina Valeria; Bellido, Demian; Wigdorovitz, Andres; Dus Santos, Maria Jose
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In a previous work, VP6 recombinant protein was produced using baculovirus system and it was evaluated in a colostrum-deprived calf model. This vaccine was able to protect calves against viral challenge without inducing neutralizing antibodies (NAb), suggesting that another immunological effectors were involved in the protection observed. In this work, groups of cows (n = 4) were immunized in the last third of gestation with a bovine rotavirus (BRV) experimental vaccine and with a VP6 subunit vaccine. At birth, colostrums from vaccinated and non-vaccinated cows were processed and viable colostral mononuclear cells were obtained. With the purpose of determining the cytokine patterns generated by cells from immune secretions (colostrums and milk), a relative quantification by real time PCR was standardized. Quantitative real time PCR (qPCR) was used to determine transcript levels of IL-4, IL-6, IL-10, IL-12, IFN-γ and IFN-α from these cells. Colostral and milk mononuclear cells expressed a different cytokine transcript expression pattern regarding the vaccine used. These results demonstrated that the colostral cellular population was active and could exert its action influencing the final immune response.
Instituto de Virología
Fil: Gonzalez, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Rimondi, Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Perez Aguirreburualde, Maria Sol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Bellido, Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Dus Santos, Maria Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fuente
Research in veterinary science 95 (2) : 703-708. (Octubre 2013)
Materia
Gene Expression
Colostrum
PCR
Cytokines
Cow Milk
Synthetic Vaccines
Expresión Génica
Calostro
Citoquinas
Leche de Vaca
Vacuna Sintética
VP6 Subunit Vaccine
Polymerase Chain Reaction
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3579
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3579
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network_name_str INTA Digital (INTA)
spelling Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with abovine rotavirus VP6 experimental vaccineGonzalez, DiegoRimondi, AgustinaPerez Aguirreburialde, Maria SolMozgovoj, Marina ValeriaBellido, DemianWigdorovitz, AndresDus Santos, Maria JoseGene ExpressionColostrumPCRCytokinesCow MilkSynthetic VaccinesExpresión GénicaCalostroCitoquinasLeche de VacaVacuna SintéticaVP6 Subunit VaccinePolymerase Chain ReactionIn a previous work, VP6 recombinant protein was produced using baculovirus system and it was evaluated in a colostrum-deprived calf model. This vaccine was able to protect calves against viral challenge without inducing neutralizing antibodies (NAb), suggesting that another immunological effectors were involved in the protection observed. In this work, groups of cows (n = 4) were immunized in the last third of gestation with a bovine rotavirus (BRV) experimental vaccine and with a VP6 subunit vaccine. At birth, colostrums from vaccinated and non-vaccinated cows were processed and viable colostral mononuclear cells were obtained. With the purpose of determining the cytokine patterns generated by cells from immune secretions (colostrums and milk), a relative quantification by real time PCR was standardized. Quantitative real time PCR (qPCR) was used to determine transcript levels of IL-4, IL-6, IL-10, IL-12, IFN-γ and IFN-α from these cells. Colostral and milk mononuclear cells expressed a different cytokine transcript expression pattern regarding the vaccine used. These results demonstrated that the colostral cellular population was active and could exert its action influencing the final immune response.Instituto de VirologíaFil: Gonzalez, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Rimondi, Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Perez Aguirreburualde, Maria Sol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Bellido, Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Dus Santos, Maria Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaElsevier2018-10-10T18:24:48Z2018-10-10T18:24:48Z2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0034528813001161http://hdl.handle.net/20.500.12123/35790034-5288http://dx.doi.org/10.1016/j.rvsc.2013.03.016Research in veterinary science 95 (2) : 703-708. (Octubre 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:19Zoai:localhost:20.500.12123/3579instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:20.124INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with abovine rotavirus VP6 experimental vaccine
title Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with abovine rotavirus VP6 experimental vaccine
spellingShingle Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with abovine rotavirus VP6 experimental vaccine
Gonzalez, Diego
Gene Expression
Colostrum
PCR
Cytokines
Cow Milk
Synthetic Vaccines
Expresión Génica
Calostro
Citoquinas
Leche de Vaca
Vacuna Sintética
VP6 Subunit Vaccine
Polymerase Chain Reaction
title_short Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with abovine rotavirus VP6 experimental vaccine
title_full Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with abovine rotavirus VP6 experimental vaccine
title_fullStr Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with abovine rotavirus VP6 experimental vaccine
title_full_unstemmed Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with abovine rotavirus VP6 experimental vaccine
title_sort Quantitation of cytokine gene expression by real time PCR in bovine milk and colostrum cells from cows immunized with abovine rotavirus VP6 experimental vaccine
dc.creator.none.fl_str_mv Gonzalez, Diego
Rimondi, Agustina
Perez Aguirreburialde, Maria Sol
Mozgovoj, Marina Valeria
Bellido, Demian
Wigdorovitz, Andres
Dus Santos, Maria Jose
author Gonzalez, Diego
author_facet Gonzalez, Diego
Rimondi, Agustina
Perez Aguirreburialde, Maria Sol
Mozgovoj, Marina Valeria
Bellido, Demian
Wigdorovitz, Andres
Dus Santos, Maria Jose
author_role author
author2 Rimondi, Agustina
Perez Aguirreburialde, Maria Sol
Mozgovoj, Marina Valeria
Bellido, Demian
Wigdorovitz, Andres
Dus Santos, Maria Jose
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Gene Expression
Colostrum
PCR
Cytokines
Cow Milk
Synthetic Vaccines
Expresión Génica
Calostro
Citoquinas
Leche de Vaca
Vacuna Sintética
VP6 Subunit Vaccine
Polymerase Chain Reaction
topic Gene Expression
Colostrum
PCR
Cytokines
Cow Milk
Synthetic Vaccines
Expresión Génica
Calostro
Citoquinas
Leche de Vaca
Vacuna Sintética
VP6 Subunit Vaccine
Polymerase Chain Reaction
dc.description.none.fl_txt_mv In a previous work, VP6 recombinant protein was produced using baculovirus system and it was evaluated in a colostrum-deprived calf model. This vaccine was able to protect calves against viral challenge without inducing neutralizing antibodies (NAb), suggesting that another immunological effectors were involved in the protection observed. In this work, groups of cows (n = 4) were immunized in the last third of gestation with a bovine rotavirus (BRV) experimental vaccine and with a VP6 subunit vaccine. At birth, colostrums from vaccinated and non-vaccinated cows were processed and viable colostral mononuclear cells were obtained. With the purpose of determining the cytokine patterns generated by cells from immune secretions (colostrums and milk), a relative quantification by real time PCR was standardized. Quantitative real time PCR (qPCR) was used to determine transcript levels of IL-4, IL-6, IL-10, IL-12, IFN-γ and IFN-α from these cells. Colostral and milk mononuclear cells expressed a different cytokine transcript expression pattern regarding the vaccine used. These results demonstrated that the colostral cellular population was active and could exert its action influencing the final immune response.
Instituto de Virología
Fil: Gonzalez, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Rimondi, Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Perez Aguirreburualde, Maria Sol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Bellido, Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Dus Santos, Maria Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
description In a previous work, VP6 recombinant protein was produced using baculovirus system and it was evaluated in a colostrum-deprived calf model. This vaccine was able to protect calves against viral challenge without inducing neutralizing antibodies (NAb), suggesting that another immunological effectors were involved in the protection observed. In this work, groups of cows (n = 4) were immunized in the last third of gestation with a bovine rotavirus (BRV) experimental vaccine and with a VP6 subunit vaccine. At birth, colostrums from vaccinated and non-vaccinated cows were processed and viable colostral mononuclear cells were obtained. With the purpose of determining the cytokine patterns generated by cells from immune secretions (colostrums and milk), a relative quantification by real time PCR was standardized. Quantitative real time PCR (qPCR) was used to determine transcript levels of IL-4, IL-6, IL-10, IL-12, IFN-γ and IFN-α from these cells. Colostral and milk mononuclear cells expressed a different cytokine transcript expression pattern regarding the vaccine used. These results demonstrated that the colostral cellular population was active and could exert its action influencing the final immune response.
publishDate 2013
dc.date.none.fl_str_mv 2013-10
2018-10-10T18:24:48Z
2018-10-10T18:24:48Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0034528813001161
http://hdl.handle.net/20.500.12123/3579
0034-5288
http://dx.doi.org/10.1016/j.rvsc.2013.03.016
url https://www.sciencedirect.com/science/article/pii/S0034528813001161
http://hdl.handle.net/20.500.12123/3579
http://dx.doi.org/10.1016/j.rvsc.2013.03.016
identifier_str_mv 0034-5288
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Research in veterinary science 95 (2) : 703-708. (Octubre 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1846143506131714048
score 12.712165

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