DNA and Non-Replicating Human Adenovirus Vectors Expressing VP2 Protein of Infectious Bursal Disease Virus Induce Humoral Immunity in the Murine Model

Autores
Romanutti, Carina; Zanetti, Flavia Adriana
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An eukaryotic expression Plasmid (pXL) and a replica- tion-defective human adenovirus serotype 5 (Ad5) carry- ing the coding sequence of mature VP2 protein from Infec- tious Bursal Disease Virus (IBDV) were constructed and the evaluation of their immunogenicity was carried out in the murine model. These novel recombinant vectors were able to express VP2 protein in cell cultures and to induce IBDV- seroneutralizing antibodies in mice vaccinated with heter- ologous prime-boost regimen. To our knowledge, this is the first report on the obtaining of a recombinant Ad5 vector expressing VP2 protein of IBDV. Other experiments will be performed to evaluate its usefulness in the chicken, natural host of IBDV.
Fil: Romanutti, Carina. Ministerio de Producción y Trabajo. Secretaría de Gobierno de Agroindustria. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Centro de Virología Humana y Animal. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Virología Humana y Animal; Argentina
Fil: Zanetti, Flavia Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina
Materia
IBDV
VP2 PROTEIN
DNA VACCINE
ADENOVIRUS VECTOR VACCINE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/181435
Acceder
id CONICETDig_69b60eff215b33ba1918242348d9b2ca
oai_identifier_str oai:ri.conicet.gov.ar:11336/181435
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling DNA and Non-Replicating Human Adenovirus Vectors Expressing VP2 Protein of Infectious Bursal Disease Virus Induce Humoral Immunity in the Murine ModelRomanutti, CarinaZanetti, Flavia AdrianaIBDVVP2 PROTEINDNA VACCINEADENOVIRUS VECTOR VACCINEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1An eukaryotic expression Plasmid (pXL) and a replica- tion-defective human adenovirus serotype 5 (Ad5) carry- ing the coding sequence of mature VP2 protein from Infec- tious Bursal Disease Virus (IBDV) were constructed and the evaluation of their immunogenicity was carried out in the murine model. These novel recombinant vectors were able to express VP2 protein in cell cultures and to induce IBDV- seroneutralizing antibodies in mice vaccinated with heter- ologous prime-boost regimen. To our knowledge, this is the first report on the obtaining of a recombinant Ad5 vector expressing VP2 protein of IBDV. Other experiments will be performed to evaluate its usefulness in the chicken, natural host of IBDV.Fil: Romanutti, Carina. Ministerio de Producción y Trabajo. Secretaría de Gobierno de Agroindustria. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Centro de Virología Humana y Animal. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Virología Humana y Animal; ArgentinaFil: Zanetti, Flavia Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología "Dr. César Milstein"; ArgentinaMedDocs2021-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/181435Romanutti, Carina; Zanetti, Flavia Adriana; DNA and Non-Replicating Human Adenovirus Vectors Expressing VP2 Protein of Infectious Bursal Disease Virus Induce Humoral Immunity in the Murine Model; MedDocs; Journal of Veterinary Medicine and Animal Sciences; 4; 1; 1-2021; 1054-10582640-1223CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://meddocsonline.org/journal-of-veterinary-medicine-and-animal-sciences-press.html#Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:57:16Zoai:ri.conicet.gov.ar:11336/181435instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:57:17.03CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv DNA and Non-Replicating Human Adenovirus Vectors Expressing VP2 Protein of Infectious Bursal Disease Virus Induce Humoral Immunity in the Murine Model
title DNA and Non-Replicating Human Adenovirus Vectors Expressing VP2 Protein of Infectious Bursal Disease Virus Induce Humoral Immunity in the Murine Model
spellingShingle DNA and Non-Replicating Human Adenovirus Vectors Expressing VP2 Protein of Infectious Bursal Disease Virus Induce Humoral Immunity in the Murine Model
Romanutti, Carina
IBDV
VP2 PROTEIN
DNA VACCINE
ADENOVIRUS VECTOR VACCINE
title_short DNA and Non-Replicating Human Adenovirus Vectors Expressing VP2 Protein of Infectious Bursal Disease Virus Induce Humoral Immunity in the Murine Model
title_full DNA and Non-Replicating Human Adenovirus Vectors Expressing VP2 Protein of Infectious Bursal Disease Virus Induce Humoral Immunity in the Murine Model
title_fullStr DNA and Non-Replicating Human Adenovirus Vectors Expressing VP2 Protein of Infectious Bursal Disease Virus Induce Humoral Immunity in the Murine Model
title_full_unstemmed DNA and Non-Replicating Human Adenovirus Vectors Expressing VP2 Protein of Infectious Bursal Disease Virus Induce Humoral Immunity in the Murine Model
title_sort DNA and Non-Replicating Human Adenovirus Vectors Expressing VP2 Protein of Infectious Bursal Disease Virus Induce Humoral Immunity in the Murine Model
dc.creator.none.fl_str_mv Romanutti, Carina
Zanetti, Flavia Adriana
author Romanutti, Carina
author_facet Romanutti, Carina
Zanetti, Flavia Adriana
author_role author
author2 Zanetti, Flavia Adriana
author2_role author
dc.subject.none.fl_str_mv IBDV
VP2 PROTEIN
DNA VACCINE
ADENOVIRUS VECTOR VACCINE
topic IBDV
VP2 PROTEIN
DNA VACCINE
ADENOVIRUS VECTOR VACCINE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv An eukaryotic expression Plasmid (pXL) and a replica- tion-defective human adenovirus serotype 5 (Ad5) carry- ing the coding sequence of mature VP2 protein from Infec- tious Bursal Disease Virus (IBDV) were constructed and the evaluation of their immunogenicity was carried out in the murine model. These novel recombinant vectors were able to express VP2 protein in cell cultures and to induce IBDV- seroneutralizing antibodies in mice vaccinated with heter- ologous prime-boost regimen. To our knowledge, this is the first report on the obtaining of a recombinant Ad5 vector expressing VP2 protein of IBDV. Other experiments will be performed to evaluate its usefulness in the chicken, natural host of IBDV.
Fil: Romanutti, Carina. Ministerio de Producción y Trabajo. Secretaría de Gobierno de Agroindustria. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Centro de Virología Humana y Animal. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Virología Humana y Animal; Argentina
Fil: Zanetti, Flavia Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencia y Tecnología "Dr. César Milstein". Fundación Pablo Cassará. Instituto de Ciencia y Tecnología "Dr. César Milstein"; Argentina
description An eukaryotic expression Plasmid (pXL) and a replica- tion-defective human adenovirus serotype 5 (Ad5) carry- ing the coding sequence of mature VP2 protein from Infec- tious Bursal Disease Virus (IBDV) were constructed and the evaluation of their immunogenicity was carried out in the murine model. These novel recombinant vectors were able to express VP2 protein in cell cultures and to induce IBDV- seroneutralizing antibodies in mice vaccinated with heter- ologous prime-boost regimen. To our knowledge, this is the first report on the obtaining of a recombinant Ad5 vector expressing VP2 protein of IBDV. Other experiments will be performed to evaluate its usefulness in the chicken, natural host of IBDV.
publishDate 2021
dc.date.none.fl_str_mv 2021-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/181435
Romanutti, Carina; Zanetti, Flavia Adriana; DNA and Non-Replicating Human Adenovirus Vectors Expressing VP2 Protein of Infectious Bursal Disease Virus Induce Humoral Immunity in the Murine Model; MedDocs; Journal of Veterinary Medicine and Animal Sciences; 4; 1; 1-2021; 1054-1058
2640-1223
CONICET Digital
CONICET
url http://hdl.handle.net/11336/181435
identifier_str_mv Romanutti, Carina; Zanetti, Flavia Adriana; DNA and Non-Replicating Human Adenovirus Vectors Expressing VP2 Protein of Infectious Bursal Disease Virus Induce Humoral Immunity in the Murine Model; MedDocs; Journal of Veterinary Medicine and Animal Sciences; 4; 1; 1-2021; 1054-1058
2640-1223
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://meddocsonline.org/journal-of-veterinary-medicine-and-animal-sciences-press.html#X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MedDocs
publisher.none.fl_str_mv MedDocs
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083109307547648
score 13.22299

Publicaciones similares